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101.
探讨活性氧(reactive oxygen species,ROS)在温石棉激活线粒体通路诱导A549凋亡中的作用。采用不同浓度温石棉粉尘染毒人肺上皮细胞(A549)后,MTT法检测细胞存活率,流式细胞术检测ROS抑制剂N-乙酰半胱氨酸(N-acetyl-L-cysteine,NAC)预处理前后的细胞凋亡率、胞内ROS水平及线粒体膜电位,Western Blot法检测各组细胞半胱天冬氨酸蛋白酶3(caspase-3)、p53蛋白表达水平。实验结果显示,温石棉可降低A549细胞存活率,呈现时间-剂量效应关系;NAC可显著减少胞内ROS水平、阻止线粒体膜电位下降,并下调caspase-3、p53蛋白的表达水平,抑制部分细胞凋亡的发生。表明ROS可通过诱导p53蛋白过表达,激活线粒体凋亡通路,诱导A549细胞发生凋亡。 相似文献
102.
Seasonal variations of phytoplankton diversity in the Coleroon coastal waters, southeast coast of India 总被引:1,自引:0,他引:1
The results of an investigation carried out during June 2005 to May 2007 on physico-chemical parameters,species composition and community structure of phytoplankton including Chlorophyll a(Chl-a) at the Coleroon coastal waters(Southeast coast of India) are reported.Air and surface water temperatures(°C) varied from 25.1 to 30.1 and from 24.5 to 28.5 respectively.Salinity values varied from 6 to 28.5 and the pH ranged between 7.0 and 8.3.Variation in dissolved oxygen content was from 3.1 to 7.5 mg/dm-3 while the light extinction coefficient values(LEC) ranged between 3.1 and 10.1 cm.The ranges of inorganic nutrients(μmol/dm-3) viz.,nitrate,nitrite,phosphate and silicate were 10.1-23.4,1.2-8.9,0.2-3.1 and 55-125,respectively.The ranges of Chlorophyll a(μg/dm-3) values was 2.0-7.5.Presently,124 phytoplankton species representing different classes viz:Bacillariophyceae(77),Dinophyceae(19),Cyanophyceae(15),Chlorophyceae(10) and Chrysophyceae(3) were recorded.The phytoplankton cell abundance varied from 0.290 to 111.662 cells/cm-3,with peak diversity(3.38 bits/ind.) during summer season.The maximum abundance was found during summer season coinciding with the stable hydrographical conditions.The seasonal distribution and abundance of phytoplankton are discussed in relation to hydrographical parameters.Canonical Correspondence Analysis(CCA) was applied in this paper for discriminating environmental factors having effect on phytoplankton community at species level.Coleroon coastal water is subjected to long term fluctuations in physico-chemical parameters depending upon the seasonal tidal amplitude and freshwater influx resulting in a continuous exchange of organic,inorganic,plant and animal matters. 相似文献
103.
胶州湾中部海域秋、冬季大型无脊椎动物群落结构及多样性研究 总被引:4,自引:1,他引:3
根据2008年9月~2009年2月在胶州湾中部海域进行的逐月定点底拖网调查,对该海域秋、冬季大型无脊椎动物群落结构及多样性变化进行了初步研究.结果表明,胶州湾秋、冬季共捕获大型无脊椎动物60种,其中甲壳动物和软体动物是主要的无脊椎动物类群;优势种组成具有较明显的月间变化,主要为口虾蛄Oratosquilla oratoria、短蛸Octopus ocellatus、脊腹褐虾Crangon affinis等湾内定居性种类及洄游性较小的种类.Margalef种类丰富度指数范围为1.998~4.669,自9月起逐月递减,11月达到最低值,随后又逐月升高,2月达到最高值;Shannon-Wiener多样性指数和Pielou均匀度指数范围分别为1.325~2.500和0.422~0.693,逐月变化趋势与物种丰富度指数基本一致.多样性指数与海域温度、盐度和深度等环境因子均无显著性相关.多元统计分析表明,胶州湾中部海域秋、冬季大型无脊椎动物群落可划分为2个时间组群;组群间群落种类组成结构差异不显著. 相似文献
104.
W. Lindsay Whitlow 《Marine Ecology》2010,31(3):418-430
Many studies on invasive species show reduced native densities, but few studies measure trait‐mediated effects as mechanisms for changes in native growth rates and population dynamics. Where native prey face invasive predators, mechanisms for phenotypic change include selective predation, or induced behavioral or morphological plasticity. Invasive green crabs, Carcinus maenas, have contributed to declines in native soft‐shell clams, Mya arenaria, in coastal New England, USA. We tested the hypothesis that clam ability to detect chemical cues from predators or damaged conspecifics would induce greater burrowing depth as a refuge from invasive crabs, and greater burrowing would require increased siphon growth. To determine how crab predation affected clam survivorship and phenotypic traits in the field, clams in exclosure, open, and crab enclosure plots were compared. Crab predation reduced clam density, and surviving clams were deeper and larger, with longer siphons. To determine whether the mechanism for these results was selective predation or induced plasticity, phenotypes were compared between clams exposed to chemical cues from crab predation and clams exposed to seawater in laboratory and field experiments. In response to crab predation cues, clams burrowed deeper, with longer siphons and greater siphon mass. Overall, crab predation removed clams with shorter siphons at shallow depths, and crab predation cues induced greater burrowing depths and longer siphons. Longer siphons and greater siphon mass of deeper clams suggests clams may allocate energy to siphon growth in response to crabs. By determining native behavior and morphological changes in response to an invasive predator, this study adds to our understanding of mechanisms for invasive impacts and illustrates the utility of measuring trait‐mediated effects to investigate predator–prey dynamics. 相似文献
105.
2009年3月,采用水下照相及摄像方法,对海南昌江沿岸海域的石珊瑚物种多样性、分布及覆盖度进行了调查.结合历史资料,共发现石珊瑚10科15属28种和1未定种.其中鹿角珊瑚属(Acropora)、角蜂巢珊瑚属(Favites)、叶状珊瑚属(Lobophyllia)等为优势类群,常见分布珊瑚种类有角孔珊瑚(Gonioporasp.)、标准蜂巢珊瑚(Favia speciosa)、五边角蜂巢珊瑚(Favites pentagona)、多孔鹿角珊瑚(Acropora millepora)、十字牡丹珊瑚(Pavona decussata)、伞房叶状珊瑚(Lobophyllia corymbosa)等.石珊瑚主要分布于海尾镇三联村以南海域,覆盖率最高为12%.本次调查在海南昌江沿岸海域记录的珊瑚种类数多于以前的记录,但少于海南南岸的三亚鹿回头,也少于北部的广西涠洲岛、广东徐闻、香港等海域,随着调查次数的增加,预计会有更多的种类被发现.该海域是北部湾珊瑚幼虫交流的中间过渡区,对珊瑚礁的保护与恢复具有重要意义. 相似文献
106.
T. Y. Lee J. C. Huang L. Y. Liao C. S. Tzeng C. H. Yang P. K. Kalita C. P. Tung 《水文研究》2012,26(24):3635-3644
Reducing or stabilizing the stream temperature of ChiChiaWan Creek is a crucial work for Formosan Landlocked Salmon because ChiChiaWan Creek is the only one habitat for this endangered species. Planting trees in the riparian zone would be one of the alternatives. The purpose of this study was to evaluate the effects of several planting strategies on daily maximum stream temperature along the river. The results showed the effective vegetative shading angles should be more than 50° along ChiChiaWan Creek to reduce the direct solar radiation heating effectively. Upstream planting with 70° vegetative shading angle could be the most effective way among all the scenarios. However, this planting strategy could not improve the worst situations in summer because of the large solar elevation angles. The upstream planting in ChiChiaWan Creek was strongly recommended because the canopies could be easier to extend to totally cover the narrow width of river producing the most effective shades. Practicing the upstream planting with 90° vegetative shading angle can increase more than 1 km long suitable habitats for the endangered Salmon in summer. Alternatively, the west‐side planting scenario was the second effective way for temperature reduction. Our result provided a useful suggestion for the authorities in charge of saving the Formosan Landlocked Salmon, particularly under the stress of global warming. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
107.
108.
中国西北C-3植物的碳同位素组成与年降雨量关系初探 总被引:2,自引:0,他引:2
本文对不同年降雨量环境下生长的藜、独行菜、魁蓟和平车前这4种常见C-3植物的δ13C进行了分析,发现这4种C-3植物的δ13C组成都表现出随年降雨量减少而变重的趋势,其中藜和独行菜的碳同位素组成对降水变化的响应相对要较其它2种植物强烈得多。藜、独行菜和魁蓟的δ13C组成与年降雨量有显著的线性关系,平车前碳同位素组成与年降雨量没有显著的相关性,表明藜、独行菜和魁蓟的δ13C组成可作为年降雨量的替代性指标,平车前的δ13C组成不能作为年降水的替代性指标。 相似文献
109.
110.
应用大气光化学模式研究了日间影响NOx光化学转化率的主要物理化学因子.探讨了在不同NMHC/NOx比值时,NOx光化学特征及其转化产物的变化规律.结果表明,影响NOx转化率的主要因子是阳光辐射强度和NMHC/NOx比值.但在NMHC/NOx比值很低时,光强的增加并不能显著提高NOx转化率.温度和初始臭氧浓度对NOx转化率的影响次之.相对湿度对NOx转化率的影响较小.在不同NMHC/NOx比值下,NOx转化特征和产物有很大区别.NMHC/NOx比值高时,产物中PAN>HNO3.NMHC/NOx比值中(低)时,产物主要是HNO3,PAN等有机氮不到10%(1%).最后初步比较了模拟和观测的NOy组成. 相似文献