裙带菜配子体基因工程选择标记的研究   总被引：1，自引：1，他引：0  

张亦陈  高江涛  张喆  姜鹏  秦松 《海洋科学》2007,31(12):64-68

研究了裙带菜(Undaria pinnatifida)配子体对除草剂草丁膦的敏感性以及温度对草丁膦处理效果的影响。结果表明,裙带菜配子体对草丁膦敏感;统计学分析得出了草丁膦在不同温度条件下处理裙带菜配子体的半致死浓度(LD50)及95%可信限;温差达到10℃时,相同处理时间各组内不同处理温度样品的LD50之间呈现显著差异,第6天的LD50由10℃组的15.78 mg/L降为20℃组的11.56 mg/L和25℃组的10.33 mg/L。研究发现可以尝试采用草丁膦作为裙带菜配子体遗传转化的选择试剂。  相似文献   

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency     

Aibin Zhan  Zhenmin Bao  Xiaoli Hu  Wei Lu  Jingjie Hu 《中国海洋大学学报(英文版)》2009,8(2):161-165

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.  相似文献   

广西合浦海草床生态系统服务功能价值评估   总被引：3，自引：0，他引：3  

韩秋影  黄小平  施平  张景平 《海洋通报(英文版)》2008,10(1):87-96

海草床生态系统是生物多样性丰富和生产力高的近岸海洋生态系统,本文以广西合浦海草床为例,结合实地调查、已有的研究成果和当地统计资料,综合运用生态经济学、资源经济学等基本理论和方法,对该地区海草生态系统的服务功能进行了价值评估。结果表明2005年该地区海草生态系统的服务功能价值为6．29×105元／a·ha,其中间接利用价值最大,为4．47×105元／a·ha,占总经济价值的70．97％;其次为非利用价值,为1．54x105元／a·ha,占总经济价值的24．52％;最少的是直接利用价值为2．84×104元／a·ha,仅占总经济价值的4．51％。  相似文献   

浅层物探技术在近海灾害地质与工程地质调查中的应用     

王舒畋 《海洋地质译丛》2008,(1):6-12

随着海洋资源的开发与滨岸工程的建设,越来越多地遇到海洋地质灾害的影响与危害。为了避免并降低海洋地质灾害的影响程度,应开展海洋灾害地质与工程地质调查。二十多年来的实践经验表明,若采用以高分辨率地震为主,配合浅地层剖面、旁侧声纳、测深及磁力测量等综合浅层物探技术,对于了解海洋地质灾害因素与海底浅部工程地质特性等方面都能取得良好的效果,这是一种既经济又较快速地提供成果资料的好方法。  相似文献   

钱塘江强潮河段江道缩窄治理研究   总被引：1，自引：0，他引：1  

陈来华  潘存鸿 《海洋工程》2008,26(2):96-102

对钱塘江强潮河段江道缩窄治理进行研究。首先,回顾治理方案的比选,江道全线缩窄时河轴线线路和河道宽度的确定原则,以及不同河段的指数放宽率。然后,介绍了在涌潮汹涌、滩涂淤坍变化剧烈的强潮河段,实施筑堤所采取的工程措施的步序与要点。最后,分析论证了大规模江道缩窄治理后河床、潮汐、洪水位及涌潮高度等特征值的变化。可供进一步深入理论研究和工程施工参考运用。  相似文献   

A traversing system to measure bottom boundary layer hydraulic properties   总被引：1，自引：0，他引：1  

Ayub Ali  Charles J. Lemckert   《Estuarine, Coastal and Shelf Science》2009,83(4):425-433

This study describes a new convenient and robust system developed to measure benthic boundary layer properties, with emphasis placed on the determination of bed shear stress and roughness height distribution within estuarine systems by using velocity measurements. This system consisted of a remotely operated motorised traverser that allowed a single ADV to collect data between 0 and 1 m above the bed. As a case study, we applied the proposed traversing system to investigate bottom boundary layer (BBL) hydraulic properties within Coombabah Creek, Queensland, Australia. Four commonly-employed techniques: (1) Log-Profile (LP); (2) Reynolds stress (RS); (3) Turbulent Kinetic Energy (TKE); and (4) Inertial Dissipation (ID) used to estimate bed shear stresses from velocity measurements were compared. Bed shear stresses estimated with these four methods agreed reasonably well; of these, the LP method was found to be most useful and reliable. Additionally, the LP method permits the calculation of roughness height, which the other three methods do not. An average value of bed shear stress of 0.46 N/m², roughness height of 4.3 mm, and drag coefficient of 0.0054 were observed within Coombabah Creek. Results are consistent with that reported for several other silty bed estuaries.  相似文献   

Estimation of the wave-related ripple characteristics and induced bed shear stress   总被引：1，自引：0，他引：1  

Benoît Camenen   《Estuarine, Coastal and Shelf Science》2009,84(4):553-564

A large data set on ripples was collected and examined. A set of new formulas for the prediction of the ripple characteristics is proposed with an emphasis on the disappearance of the ripples. The ripple wavelength was observed to be proportional to the bottom wave excursion but also to be a function of the grain-related Shields parameter and wave period parameter introduced by Mogridge et al. (1994). The ripple steepness was found to be nearly constant for orbital ripples, and with a sharp decrease for suborbital ripples. Two empirical functions are added including the effects of the critical Shields parameters (inception of transport and inception of sheet flow), i.e. giving the boundaries for the ripple existence's domain. The proposed formulas yield better prediction capabilities compared to the previously published formulas, especially when ripples are washed out. The effect of the ripple characteristics on the roughness height and the calculation of the bed shear stress is also discussed. It appeared that the bed shear stress calculation is more sensitive to the empirical coefficient a_r introduced in the estimation of the ripple-induced roughness height or to the limits of existence of the ripples than the ripple characteristics themselves.  相似文献   

海洋动床物理模型试验地形制作技术   总被引：2，自引：2，他引：0  

刘自力  刘学海  纪育强  马小兵  于凯本  毕忠野 《海岸工程》2007,26(3):42-46

试验模型地形制作是物模试验的一个基本工作,模型制作的好坏直接关系到实验的精度.模型试验之前,需要根据相似理论,选取合适的材料,按照一定的几何比尺进行模型的制作.通过2个具体工程作为实例说明了模型制作的过程、制作的技术性问题和制作经验.  相似文献   

黄颡鱼(Pelteobagrus eupogon)不同生态地理分布群体遗传多样性的微卫星分析   总被引：1，自引：0，他引：1  

李大宇  殷倩茜  侯宁  孙效文  梁利群 《海洋与湖沼》2009,40(4):460-469

利用40个黄颡鱼微卫星分子标记对吉林省月亮湖野生黄颡鱼(YL,30尾)、四川野生黄颡鱼(SC,30尾)、黑龙江省哈尔滨市松花江段黄颡鱼(SH,18尾)、湖北省荆州市长湖野生黄颡鱼子代(YY,30尾)及其亲本(QQ,30尾)、天津市换新国家级原良种场黄颡鱼1号(TJ,30尾)6个黄颡鱼群体的观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)和有效等位基因数(Ae)等进行了遗传检测,根据基因频率计算遗传相似系数和Nei氏标准遗传距离,x2检验估计Hardy-weinberg平衡,采用近交系数(FST)和基因流(Nm)分析群体的遗传分化及其来源.同时,使用PHYLIP3.63软件绘制基于Nei氏标准遗传距离的UPGMA进化树.6个群体共检测到5042个扩增片段,长度在150-650bp,40个基因座扩增出等位基因数从1-8个不等,共计143个等位基因,平均每个基因座扩增得到3.575个等位基因.结果显示:(1)6个黄颡鱼群体的多态性指标均适中,PIC在0.00-0.79之间,平均值为0.40、0.37、0.35、0.39、0.35和0.19,有效等位基因数(Ae)1.00-5.44个不等,平均有效等位基因数依次为2.05、2.30、1.93、2.07、1.93和1.47,无偏期望杂合度(He)在0.00-0.83之间,平均值为0.45、0.46、0.36、0.46、0.41和0.23;(2)遗传相似系数YY与QQ遗传相似度最高(0.9947),TJ与SC遗传相似系数最低(0.4846),聚类结果与地理分布呈一定相关性.  相似文献   

Numerical modeling of nonlinear water waves over heterogeneous porous beds   总被引：1，自引：0，他引：1  

Eric C. Cruz  Qin Chen   《Ocean Engineering》2007,34(8-9):1303-1321

The transformation of nonlinear water waves over porous beds is studied by applying a numerical model based on Chen's [2006. Fully nonlinear Boussinesq-type equations for waves and currents over porous beds. Journal of Engineering Mechanics, 132:2, 220–230] Boussinesq-type equations for highly nonlinear waves on permeable beds. The numerical model uses a high-order time-marching solution and fourth-order finite-difference schemes for discretization of first-order spatial derivatives to obtain a computational accuracy consistent with the model equations. By forcing the wave celerity and spatial porous-damping rate of the linearized model to match the exact linear theory for horizontal porous bed over a prescribed range of relative depths, the values of the model parameters are optimally determined. Numerical simulations of the damped wave propagation over finite-thickness porous layer demonstrate the accuracy of both the numerical model and governing equations, which have been shown by prior theoretical analyses to be accurate for both nominal and thick porous layers. These simulations also elucidate on the significance of the higher-order porous-damping terms and the influence of the hydraulic parameters. Application of the model to the simulation of the wave field around a laboratory-scale submerged porous mound provides a measure of its capability, as well as useful insight into the scaling of the porous-resistance coefficients. For application to heterogeneous porous beds, the assumption of weak spatial variation of the porous resistance is examined using truncated forms of the governing equations. The results indicate that the complete set of Boussinesq-type equations is applicable to porous beds of nonhomogeneous makeup.  相似文献