九孔鲍BMP-2基因cDNA克隆及表达分析     

艾加林  栗志民  刘建勇  申玉春 《海洋科学》2018,42(8):107-115

为探究BMP-2基因在九孔鲍(Haliotisdiversicolorsupertexta)中的功能,采用cDNA末端快速扩增(RACE)技术从九孔鲍外套膜中获得了BMP-2基因cDNA全长,并用实时荧光定量PCR(qRT-PCR)检测了BMP-2基因在各组织和发育时期的表达水平。结果表明:九孔鲍BMP-2基因cDNA全长2572bp,其中5′非编码区(5′UTR)123bp, 3′非编码区(3′UTR)1150bp,开放阅读框(ORF)为1299 bp,编码432个氨基酸,其分子质量为48.59ku,理论等电点(pI)为9.84;具有N端信号肽(1-39 aa)、TGF-β前肽区域(63-294 aa)和TGF-β成熟肽区域(331-432 aa),以及蛋白酶水解位点RLRR (272-275 aa)和7个保守的半胱氨酸残基,符合TGF-β超家族蛋白典型结构特征。系统进化树结果显示九孔鲍BMP-2基因和耳鲍聚为一支。qRT-PCR结果表明BMP-2基因在九孔鲍的6个组织中均有表达,在足、右侧壳肌、外套膜及肝脏中显著高表达;在检测的7个发育时期均表达,其中在受精卵、4细胞、8细胞、原肠胚、稚鲍时期表达量显著高于卵、幼鲍时期。研究表明BMP-2基因可能在九孔鲍贝壳形成中具有重要作用。  相似文献   

潮流界以下河段航道整治水位确定方法研究——以长江江阴以下河段为例     

徐元  贾雨少 《海洋工程》2018,36(2):64-72

在总结分析现有整治水位确定方法的基础上,根据潮流界以下河段的水沙运动特性,本文提出一种基于输沙能力的航道整治水位确定方法。考虑上游来水、下游潮汐为独立事件,统计潮流界以下河段上游来水、下游潮汐不同等级组合出现的频率,采用数学模型计算相应组合下河段沿程的潮位、流速过程,以流速四次方代表水流的输沙能力,统计不同潮位等级对应的综合净输沙能力,确定最大综合净输沙能力对应的水位为(最优)航道整治水位。以长江下游白茆沙水道和福姜沙水道为例,计算了所在河段的航道整治水位,并探讨了起动流速对整治水位计算的影响和最高整治水位概念对工程的意义。  相似文献   

Population connectivity of an overexploited coastal fish,Argyrosomus coronus (Sciaenidae), in an ocean-warming hotspot     

R Henriques  WM Potts  CV Santos  WHH Sauer  PW Shaw 《African Journal of Marine Science》2018,40(1):13-24

The West Coast dusky kob Argyrosomus coronus is a commercially exploited fish with a distribution confined to the Angola–Benguela Frontal Zone (ABFZ) of the southeastern Atlantic Ocean. A previous study revealed that during a recent period of local warming the species extended its distribution into Namibian waters, where it hybridised with the resident and congeneric Argyrosomus inodorus. Environmental changes are a major threat to marine biodiversity and when combined with overfishing have the potential to accelerate the decline of species. However, little is known regarding the evolutionary history and population structure of A. coronus across the ABFZ. We investigated genetic diversity, population structure and historical demographic changes using mtDNA control region sequences and genotypes at six nuclear microsatellite loci, from 180 individuals. A single, genetically homogeneous population was indicated across the distributional range of A. coronus (φ_ST = 0.041, F_ST = 0.000, D = 0.000; p > 0.05). These findings imply that the oceanographic features within the ABFZ do not appear to significantly influence population connectivity in A. coronus, which simplifies management of the species. However, reconstruction of the demographic history points to a close link between the evolutionary history of A. coronus and the environmental characteristics of the ABFZ. This outcome suggests the species’ vulnerability to the rapid environmental changes being observed across this region, and highlights a pressing need for transboundary management to mitigate the impacts of climate change in this global hotspot of seawater temperature changes.  相似文献   

银纳米杆高温熔化断裂弛豫性能的原子级模拟研究     

卢敏  许卫兵  刘维清  侯春菊  刘志勇 《海洋学报》2010,32(9):6377-6383

采用分子动力学模拟方法,研究了不同长度银纳米杆在不同温度弛豫过程中的结构演变过程.结果表明:银纳米杆存在一与杆长相关的临界熔断温度,该临界熔断温度随杆长增加而显著降低.当温度大于熔点而小于临界熔断温度时,体系形成一个高度无序的球形团簇,而温度大于临界熔断温度时,体系则熔断成两个球形团簇.并给出了银纳米杆的产生该熔断现象的机理.  相似文献   

原子掺杂对单元材料结晶能力的影响     

彭坤  明辰  叶祥熙  张文献  庄军  宁西京 《海洋学报》2010,32(10):7245-7251

2009年我们建立了一个凝结势模型用以预测材料形成单晶体的能力,表明单元体材料(Ni,Al,Cu,Ar,Mg)的结晶能力随凝结势的增大而单调增强.本文将凝结势模型应用于二元材料体系,并结合分子动力学模拟研究了6% Al原子掺杂对于Ni单晶材料结晶能力的影响.模拟结果发现,Al元素的掺杂会大大减弱Ni单晶的结晶能力,在此基础上提出了二元材料体系凝结势的定义,表明凝结势模型可广泛应用于预测二元体材料的结晶能力.  相似文献   

Accurate ab initio study of low-lying electronic states of phosphorus nitride radical     

王杰敏  孙金锋  施德恒 《海洋学报(英文版)》2010,29(11)

This paper employs the highly accurate valence internally contracted multireference configuration interaction method to investigate the potential energy curves (PECs) for the ground state (X¹Σ⁺) and two low-lying excited states (A¹Π and D¹Δ of phosphorus nitride (PN) radical with the correlation-consistent basis set, aug-cc-pV6Z, in the valence range. Relativistic effects are considered in these calculations. The spectroscopic constants of the X¹Σ⁺ and A¹Π states are calculated based on the PECs, and the results are in good accord with the available experimental data. The first 30 vibrational states for the A¹Π state and the first 40 vibrational states for the A¹Π state are determined when J=0. For each vibrational state, molecular constants G(υ), B(υ) and D(υ) are also attained.  相似文献   

LMWOA (low molecular weight organic acid) exudation by salt marsh plants: Natural variation and response to Cu contamination     

Ana P. Mucha  C. Marisa R. Almeida  Adriano A. Bordalo  M. Teresa S.D. Vasconcelos 《Estuarine, Coastal and Shelf Science》2010

This work aimed to evaluate, in vitro, the capability of roots of two salt marsh plants to release low molecular weight organic acids (LMWOAs) and to ascertain whether Cu contamination would stimulate or not organic acids exudation. The sea rush Juncus maritimus and the sea-club rush Scirpus maritimus, both from the lower Douro river estuary (NW Portugal), were used. Plants were collected seasonally, four times a year in 2004, during low tide. After sampling, plant roots were washed for removal of adherent particles and immersed for 2 h in a solution that matched salinity (3) and pH (7.5) of the pore water from the same location to obtain plant exudates. In one of the seasons, similar experiments were carried out but spiking the solution with different amounts of Cu in order to embrace the range between 0 and 1600 nM. In the final solutions as well as in sediment pore water LMWOAs were determined by high performance liquid chromatography. Plants were able to release, in a short period of time, relatively high amounts of LMWOAs (oxalate, citrate, malate, malonate, and succinate). In the sediment pore water oxalate, succinate and acetate were also detected. Therefore, plant roots probably contributed to the presence of some of these organic compounds in pore water. Exudation differed between the plant species and also showed some seasonally variation, particularly for S. maritimus. The release of oxalate by J. maritimus increased with Cu increase in the media. However, exudation of the other LMWOAs did not seem to be stimulated by Cu contamination in the media. This fact is compatible with the existence of alternative internal mechanisms for Cu detoxification, as denoted by the fact that in media contaminated with Cu both plant species accumulated relatively high amounts (29–83%) of the initially dissolved Cu. This study expands our knowledge on the contribution of globally dominant salt marsh plants to the release of LMWOAs into the environment.  相似文献   

Institutions as facilities for change?: A study of the coherence between political regulations and innovations within the pelagic fisheries sector in Norway     

Stig-Erik Jakobsen  Bernt Aarset 《Marine Policy》2010

Political regulation has traditionally been regarded as antagonistic to innovation, whereas the removal of regulations has been perceived as a precondition for innovations. This paper proposes an alternative position and claim that regulations can have various connections with the innovation process. Regulations can obviously restrict innovations, but they can also facilitate such processes. Systematic empirical research on the relation between political-administrative regulation and innovation has been moderate, and the debate has been highly anecdotal. This article presents an empirical study on how political-administrative regulation within the pelagic fisheries sector in Norway affects innovation. The study confirms that regulations had varied influences on the innovation process, and documents that actor's appraisals of regulations were affected by whether a given regulation is beneficial for the firms and their competitive position or not. Finally, the paper corroborate that the implementation of regulations can promote innovation in firms by providing them with deeper insights into their production processes and to encourage a more intense focus on development and improvement. Implementation of regulations also implies learning, which can increase the firms’ abilities to innovate as well as their insight into the necessity of such practices. The analysis is based on survey data from processors and vessels in the pelagic sector.  相似文献   

珠江磨刀门水道咸潮上溯加剧的原因   总被引：1，自引：0，他引：1  

韩志远  田向平  刘峰 《海洋学研究》2010,28(2):52-59

对磨刀门水道不同年份地形资料和多年枯季潮位资料进行了对比分析,结果表明:大规模的河床采砂,引起磨刀门上游河段河床大幅下切,河道深泓平均加深0.59~2.25 m,河槽容积平均增加8.91×106~12.11×106m3,使该河段枯季潮差增大,进潮量增加,潮汐动力增强,导致咸水界向上游河段推移;口门围垦整治后,磨刀门口门区由众多岛屿环抱而成的内海区变成人工导堤控制的"一主一支"两条水道,水域面积减少了80%,河槽容积减少了40%,深槽趋于顺直加深,使口门区的"调淡"作用消失,这是咸潮上溯的重要原因;根据多年水文频率分析,发生严重咸潮灾害时,西、北江枯季流量并非都是枯水流量,因此枯季上游来水量变化并不是近年来咸潮灾害加剧的主要原因。  相似文献   

环境规制对京津冀工业发展的门槛效应     

王磊  王琰琰  张宇 《地域研究与开发》2019,38(1):132-137

  相似文献