EFFECT OF LIGHT INTENSITY ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS     

梁英麦康森  孙世春于道展 《中国海洋湖沼学报》2001,19(3):249-254

The effect of light intensity ( 1500 Ix and 5000 Ix) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211 ), Phaeodactylum tricornutum (Bl14, Bl18 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated.The total lipids of B13, Bl14, and B211 grown at 5000 Ix were lower than those grown at 1500 Ix. No evident changes were observed in Bl18, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14:0, 16:0, 16:l(n- 7) and 20:5(n-3) . Cylindrothecafusiformis had high percentage of 20:4n - 6 (9.2 - 10.9% ) . The total polyunsaturated fatty acid /rl all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.  相似文献   

圆盘固相萃取富集-气相色谱法测定地表水中有机氯和有机磷农药   总被引：4，自引：2，他引：2  

何淼  饶竹 《岩矿测试》2008,27(1):12-16

采用环境友好的圆盘固相萃取新技术富集水体中有机氯农药和有机磷农药,分别用微池电子捕获检测器(μECD)和火焰光度检测器(FPD)气相色谱法检测,实现了水中有机氯和有机磷农药残留物的测定。结果表明,16种有机氯农药的平均回收率为64.7%~102%,精密度(RSD,n=6)为2.9%~15%;13种有机磷农药的平均回收率为65.9%~104%,精密度(RSD,n=6)为1.7%~17%。方法快速、灵敏、低污染,可用于水体中多种有机氯农药和有机磷农药的残留分析。  相似文献   

Phaeocystis globosa与Phaeocystis antarctica叶绿体psbA基因的比较   总被引：3，自引：0，他引：3       下载免费PDF全文

杨泽民  章群  谢数涛  韩博平  吕颂辉  Hodgkiss 《海洋科学》2005,29(1):24-28

测定了2株球形棕囊藻Phaeocystis globosa P1、P2的psbA基因序列。发现得到的2个序列完全相同。以P2序列对比分析了P．globosa和P．antarctica的psbA基因在DNA序列、氮基酸序列和RNA二级结构上的差异性，发现2种棕囊藻psbA基因DNA序列和氨基酸序列非常保守，无插入／缺失，其核苷酸和氨基酸变异率分别为1．88％和1．13％。与核基因核苷酸的碱基替换不同，psbA基因核苷酸的碱基替换主要发生在密码子的第1位上。且不引起氨基酸的变化，引起氨基酸变化的碱基替换都发生在密码子的第2位和第3位上。在RNA二级结构上两序列的1～4茎环结构完全相同，表现出明显的棕囊藻属的特异性，其它结构区域差异较大。种间差异表现明显。由于psbA基因DNA序列和氨基酸序列非常保守，可能不适宜棕囊藻属的系统发育分析。但其RNA二级结构可能对于棕囊藻的分子分类有一定的参考价值。  相似文献   

Control strategies for the Clam Wave Energy Device   总被引：1，自引：0，他引：1  

Martin Greenhow  Jay H. Rosen  Mark Reed 《Applied Ocean Research》1984,6(4):197-206

A promising wave energy device being currently investigated is the ‘clam’. The clam extracts energy by pumping air through a specially designed (Wells) turbine. Although operation of the Wells turbine does not require a rectified air flow, some additional control will be necessary to optimize the phase of the clam motion for good efficiencies. An examination of the equation of motion in the time domain suggests the possibility of phase control by mechanical, power take-off, or pneumatic latching. Latching can be shown to increase the efficiency of the device in the longer wavelengths of the wave spectrum, i.e. those of high incident wave power. Equivalently latching could be used to keep the device efficiency high while reducing its size, possibly resulting in cheaper power extraction.  相似文献   

海带膳食纤维的提取与功能性试验   总被引：2，自引：1，他引：2  

李来好  陈培基  李刘冬  杨贤庆  吴燕燕  刁石强  薛长湖 《中国海洋大学学报(自然科学版)》2003,33(5):687-694

采用酶技术和化学处理相结合的方法 ,研究了海带膳食纤维的工艺技术 ,筛选出海带膳食纤维提取和漂白的最佳工艺条件 ,提取出海带膳食纤维产品 ,并进行人体的功能性试验。结果表明 ,海带膳食纤维的产率达 2 7.3% ,颜色为类白色 ,膳食纤维含量达 73.3% ,钙含量 7.5% ,膨胀力为55m L/ g,持水力为 2 650 % ;海带膳食纤维的功能性优于小麦麸皮膳食纤维 ,对便秘患者具有良好的疗效。  相似文献   

海水中234Th的超低水平液闪谱仪测定   总被引：2，自引：1，他引：2  

李鸿宾  黄奕普  陈敏  刘广山  邱雨生 《台湾海峡》2004,23(2):199-207

本文提出了利用超低水平液闪谱仪测定海水中234Th的方法.海水经氢氧化铁吸附共沉淀富集后,接着用阴离子交换和TBP/煤油萃取进行Th同位素的分离与纯化.对一系列测定条件进行了详细的研究,提出了测定海水中234Th的适宜程序,即在含有234Th和产额示踪剂230Th的纯化后的5mol/dm3HNO3溶液中加入TBP/煤油进行萃取,然后用0.1mol/dm3HNO3反萃取,后者是先用契伦柯夫计数法测量234Th(通过234mPa),后加入闪烁液Hisafe 3用α/β模式测量α放射体230Th.对于α和β放射体液闪谱仪的计数效率分别为100%和55.7%±2.7%.234Th的化学回收率和总探测效率分别为70%～80%和30%～45%.该法测定海水中的234Th快速、简便和高效.  相似文献   

Response of the barrier island coastal region of southwestern Nigeria to climate and non-climate forcing     

IB Danladi  M Gül  E Ateş 《African Journal of Marine Science》2020,42(1):43-51

Despite threats emanating from the influence of climate and non-climate forcing on the barrier island coastal region of southwestern Nigeria, the extent of the coastal erosion is poorly understood. We report evidence of coastal erosion and sediment accumulation in the region over a 34-year period (1973–2017), using Landsat imagery at intervals of approximately six years. Landsat image corrections and various water-extraction algorithms were used to systematically delineate coastal erosion and accumulation in the area. The region was subdivided into western and eastern subregions separated by Lagos Harbour. In the west, erosion took place during the periods 1973–1979, 1979–1984, 1990–1999 and 2005–2011, whereas in the east, erosion occurred during 1973–1979, 1990–1999 and 1999–2005. Coastal sediment accumulation occurred in the east during 1979–1984, 1984–1990, 2005–2011 and 2011–2017, whereas gains in the west occurred during 1984–1990, 1999–2005 and 2011–2017. The study revealed substantial net erosion of 1 228.1 ha in the region as a whole, over the full period. Sediment accumulation accompanying the coastal erosion appears to be linked to longshore drift. Erosion between 1973 and 2011 was probably attributable to climate change (storms and tidal conditions), longshore drift, the inflow and outflow of water at Lagos Harbour, coastal morphology and, possibly, human impacts. However, the coastal changes between 2011 and 2017 were more obviously associated with human activities, such as development of the Eko Atlantic construction project. Coastal surveillance, together with the use of environmentally sensitive protective measures, could possibly help to reduce coastal erosion in the region. Careful coastal management practices, including artificial nourishing and the installation of resilient structures (e.g. seawalls), should be undertaken to protect human settlements that are already at risk from sea-level rise.  相似文献   

对虾养殖生态系中有机碳的初步研究本研究由   总被引：1，自引：0，他引：1  

游奎  马甡 《中国海洋大学学报(自然科学版)》2002,32(1):51-55

对于围隔养殖对虾生态系中有机碳的变动进行了研究 ,结果表明 :溶解有机碳 (DOC)含量波动在 5 .2 99～ 13.39mg/ L之间 ,平均为 8.5 3mg/ L± 2 .2 5 mg/ L;颗粒有机碳 (POC)含量波动在0 .6 5～ 6 .6 3mg/ L之间 ,平均为 3.2 5 mg/ L± 1.76 mg/ L;总有机碳 (TOC)含量波动在 6 .92～ 2 0 .0 2mg/ L之间 ,平均为 11.78mg/ L± 3.82 mg/ L ;其中 DOC∶ POC∶ TOC为 0 .72∶ 0 .2 8∶ 1。各种有机碳组分的含量明显高于自然海水中各种有机碳的含量 ;各种有机碳组分的含量在养殖期间总体上呈上升的趋势 ;其变化与 DCOD的变化呈显著线性正相关的关系。  相似文献   

Phylogenetic analysis of Pectinidae(Bivalvia) based on the ribosomal DNA internal transcribed spacer region     

HUANG Xiaoting  BI Ke  HU Jingjie  HU Xiaoli  BAO Zhenmin 《海洋学报(英文版)》2007,26(6):83-90

The ribosomal DNA internal transcribed spacer (ITS) region is a useful genomic region for understanding evolutionary and genetic relationships. In the current study, the molecular phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) was performed using the nucleotide sequences of the nuclear ITS region in nine species of this family. The sequences were obtained from the scallop species Argopecten irradians, Mizuhopecten yessoensis, Amusium pleuronectes and Mimachlamys nobilis, and compared with the published sequences of Aequipecten opercularis, Chlamys farreri, C. distorta, M. varia, Pecten maximus, and an outgroup species Perna viridis. The molecular phylogenetic tree was constructed by the neighbor-joining and maximum parsimony methods. Phylogenetic analysis based on ITS1, ITS2, or their combination always yielded trees of similar topology. The results support the morphological classifications of bivalve and are nearly consistent with classification of two subfamilies (Chlamydinae and Pectininae) formulated by Waller. However, A. irradians, together with A. opercularis made up of genera Amusium, evidences that they may belong to the subfamily Pectinidae. The data are incompatible with the conclusion of Waller who placed them in Chlamydinae by morphological characteristics. These results provide new insights into the evolutionary relationships among scallop species and contribute to the improvement of existing classification systems.  相似文献   

富钴结壳分布的分形特征   总被引：8，自引：0，他引：8  

何高文 《海洋地质与第四纪地质》2001,21(1):89-92

利用我国在西太平洋国际海底两个海山获得的富钴结壳资料进行分形研究后发现，富钴结壳的分布具有变维分形分布的特点，结壳丰度呈二阶累计和分形分布，结壳厚度呈多阶不定累计和分形分布，通过计算得到了它们各自的分维数，进而对两个海山的结壳资源进行了初步评价。  相似文献