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81.
Eight marine-derived fungi that were previously selected for their abilities to decolorize RBBR dye were subjected to pyrene and benzo[a]pyrene degradation. The fungus Aspergillus sclerotiorum CBMAI 849 showed the best performance with regard to pyrene (99.7%) and benzo[a]pyrene (76.6%) depletion after 8 and 16 days, respectively. Substantial amounts of benzo[a]pyrene (>50.0%) depletion were also achieved by Mucor racemosus CBMAI 847. Therefore, these two fungal strains were subjected to metabolism evaluation using the HPLC-DAD-MS technique. The results showed that A. sclerotiorum CBMAI 849 and M. racemosus CBMAI 847 were able to metabolize pyrene to the corresponding pyrenylsulfate and were able to metabolize benzo[a]pyrene to benzo[a]pyrenylsulfate, suggesting that the mechanism of hydroxylation is mediated by a cytochrome P-450 monooxygenase, followed by conjugation with sulfate ions. Because these fungi were adapted to the marine environment, the strains that were used in the present study are considered to be attractive targets for the bioremediation of saline environments, such as ocean and marine sediments that are contaminated by PAHs. 相似文献
82.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants in the environment and are derived from both man-made and natural resources. The present study is focused on the degradation of PAHs by a halotolerant bacterial strain under saline conditions. The bacterial strain VA1 was isolated from a PAH-degrading consortium that was enriched from marine water samples that were collected from different sites at Chennai, India. In the present study, a clearing zone formed on PAH-amended mineral salt agar media confirmed the utilization of PAH by the bacterial strain VA1. The results show that the strain VA1 was able to degrade anthracene (88%), phenanthrene (98%), naphthalene (90%), fluorene (97%), pyrene (84%), benzo(k)fluoranthene (57%) and benzo(e)pyrene (50%) at a 30 g/L NaCl concentration. The present study reveals that the VA1 strain was able to degrade PAHs in petroleum wastewater under saline conditions. The promising PAH-degrading halotolerant bacterial strain, VA1, was identified as Ochrobactrum sp. using biochemical and molecular techniques. 相似文献
83.
Rhodococcus sp. P14 was isolated from crude oil-contaminated sediments. This strain was capable of utilizing three to five rings polycyclic aromatic hydrocarbons (PAHs) including phenanthrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) as a sole carbon and energy source. After cultivated with 50mg/L of each PAH, strain P14 removed 43% Phe, 34% Pyr and 30% BaP in 30 d. Four different hydroxyphenanthrene products derived from Phe by strain P14 (1,2,3,4-hydroxyphenanthrene) were detected using SPME-GC-MS. Strain P14 also was capable of degrading mineral oil with n-alkanes of C17 to C21 carbon chain length. Compared with glucose-grown cells, PAHs-grown cells had decreased contents of shorter-chain length fatty acids (≤ C16:0), increased contents of C18:0, Me-C19:0 and disappeared odd-number carbon chain fatty acids. The contents of unsaturated C19:1, Me-C19:0 increased and C18:0 decreased in mineral oil-grown cells. At the same time, the strain P14 tended to float when cultivated in mineral oil-supplemented liquid medium. The degradation capability of P14 to alkane and PAHs and its floating characteristics will be very helpful for future's application in oil-spill bioremediation. 相似文献
84.
Barbora Topinkova Kamil Nesetril Josef Datel Ondrej Nol Petr Hosl 《Hydrogeology Journal》2007,15(5):961-976
The geochemical processes, water–rock interactions and stable isotopes distribution (δ13C of DIC and δ18O and δ34S of \({\text{SO}}^{{{\text{2 - }}}}_{{\text{4}}} \)) were investigated in the gasoline-contaminated aquifer at the Hnevice site, 50 km northwest of Prague, Czech Republic. Diesel, gasoline and oil leaks originate from a large fuel storage area causing heavy contamination of the saturated and unsaturated zones in an area of about 0.7 km2. Groundwater investigations were conducted using five multilevel sampler wells with emphasis on redox parameters and degradation by-products and a solid-phase study focused on iron speciation and determination of principal and secondary minerals. Based on the study of groundwater and solid-phase geochemistry, four different geochemical zones were described. Zone I is thought to be background consisting of an aerobic aquifer and the absence of reduced species in significant concentrations. Zone II is situated in the plume core with methanogenic, sulphate and iron-reducing conditions accompanied by ankerite and kutnahorite precipitates and significant depletion of the oxidation capacity of the aquifer. Zone III is a mixing (corona) zone, situated at the fringe of the plume with high biodegradation rates and Fe(III)-precipitants. In zone IV, reoxidation of Fe(II) minerals (with e.g. the occurrence of psilomelane and cornelite) is typical. 相似文献
85.
E. Denecke 《洁净——土壤、空气、水》1997,25(6):311-318
Evaluation of Long-term Measurements Concerning the Aerobic Degradation Performance of the Subsoil Passage of a Water Catchment at the Lower Rhine The magnitude of the biodegradation of organics during bank filtration was investigated and exemplary quantified at a drinking water catchment in the lower river Rhine area. The catchment is situated at an undercut slope where the Rhine has a steady flow. The observed segment of the quarternary aquifer was free of iron and manganese and showed even under anaerobic conditions no heterotrophic denitrification. This facilitated the assignment and the balance of oxygen consumption and the determination of inorganic carbon due to the metabolic activity of heterotrophic organisms. In particular the progressive curve of the base neutralization capacity up to pH 8.2 of the bank filtered water proved to be a good measure for the biological activity in the river bottom. In the months from summer to autumn there was an increased biological activity. The observed oxygen consumptions could be attributed to the biodegradation of dissolved organic matter by 35 to 45% only. Obviously the particulate organic matter after the rise in temperature during summer takes part in a substantial manner considered little until now. Today the Rhine is regarded as a largely rehabilitated river with respect to oxygen consuming pollutions. The observations demonstrate, that in such flowing waters the particulate organic matter, which should mainly consist of withered organisms, may have a dominant influence on the concentration of oxygen in the bank-filtered water in summertimes. 相似文献
86.
Alexander Chakhmakhchev Masaru Suzuki Amane Waseda Kuniaki Takayama 《Organic Geochemistry》1997,27(7-8)
Siliceous sourced Tertiary oils from the Circum-Pacific area of Japan, Russia and the U.S.A. have a heavy carbon isotope composition, monomodal n-alkane distributions, and nearly identical regular sterane compositions with a predominance of C27 homologues. These are consistent with open marine depositional environments dominated by diatomaceous organic matter. However, a number of alkane and biomarker parameters such as Pr/Ph, CPI, relative concentration of 28,30-bisnorhopane, and the C35/C34 homohopane ratio indicate more oxic depositional environments for the source rocks of Japan and Russia. In contrast to the California Monterey Formation sourced oils, petroleums with low maturity levels from the North Sakhalin basin, Russia and the Akita basin, Japan have lower concentrations of asphaltenes and sulphur and are characterized by higher API gravities. A correlation of extractable organic matter from source rocks vs the least matured petroleums demonstrates that oil expulsion in siliceous shales of the Akita basin occurs at a maturity level corresponding to Ro≥0.65%, which is in the range of the conventional oil window (Ro = 0.6−1.1%). 相似文献
87.
Field observations on the variability of crude oil impact on indigenous hydrocarbon-degrading bacteria from sub-Antarctic intertidal sediments 总被引：3，自引：0，他引：3
Oil pollution of the oceans has been a problem ever since man began to use fossil fuels. Biodegradation by naturally occurring populations of micro-organisms is a major mechanism for the removal of petroleum from the environment. To examine the effects of crude oil pollution on intertidal bacteria, we repeated the same contamination experiments on nine different sub-Antarctic intertidal beaches using specifically built enclosures (PVC pipe, 15 cm in inner diameter and 30 cm in height). Despite the pristine environmental conditions, significant numbers of indigenous hydrocarbon-degrading bacteria were observed in all the studied beaches. Introduction of oil into these previously oil-free environments resulted in several orders of magnitude of increase in hydrocarbon-degrading micro-organisms within a few days in some of the studied sites but has no obvious effects on two others. The physical environment of the bacterial assemblage seems to play a major role in the biodegradation capacities. After 3 months of contamination, both remaining oil concentrations and biodegradation indexes differ strongly between the different stations. Thus, chemical and biological parameters reveal a strong heterogeneity of biodegradation capacities between the different sites. 相似文献
88.
Eighteen models with different levels of complexity for representing sorption, mass transfer, and biodegradation are used to simulate the biodegradation of toluene (primary substrate) and TCE (cometabolic substrate). The simulations are conducted for hypothetical completely mixed systems of various scenarios with regard to sorbent, microbial composition, and solute concentrations. The purpose of the suite of simulations is to investigate the sensitivity of different modeling approaches in simulating the bio-attenuation of co-existing solutes in sorbent-water systems. The sensitivity of results to the modeling approach depends on the biogeochemical conditions of the system. For example, the results are insensitive to the type of sorption model in systems with low sorption strength and slow biodegradation rates, and insensitive to the biodegradation rate model if mass transfer controlled. Differences among model results are generally greater when evaluated in terms of total mass removal rather than aqueous phase concentration reduction. The fate of the cometabolite is more sensitive to the proper consideration of co-solute effects than is the fate of the primary substrate. For a given system, graphical comparison of a characteristic mass transfer rate coefficient (αmt) versus a characteristic biodegradation rate coefficient (αbio) provides an indication of how sensitivity to the different processes may be expected to change with time and can guide the selection of an appropriate level of model complexity. 相似文献
89.
90.
Mao-Cheng Deng Jing Li Fu-Rui Liang Meisheng Yi Xiao-Ming Xu Jian-Ping Yuan Juan Peng Chou-Fei Wu Jiang-Hai Wang 《Marine pollution bulletin》2014
Microorganisms play an important role in the biodegradation of petroleum contaminants, which have attracted great concern due to their persistent toxicity and difficult biodegradation. In this paper, a novel hydrocarbon-degrading bacterium HZ01 was isolated from the crude oil-contaminated seawater at the Daya Bay, South China Sea, and identified as Achromobacter sp. Under the conditions of pH 7.0, NaCl 3% (w/v), temperature 28 °C and rotary speed 150 rpm, its degradability of the total n-alkanes reached up to 96.6% after 10 days of incubation for the evaporated diesel oil. Furthermore, Achromobacter sp. HZ01 could effectively utilize polycyclic aromatic hydrocarbons (PAHs) as its sole carbon source, and could remove anthracene, phenanthrene and pyrence about 29.8%, 50.6% and 38.4% respectively after 30 days of incubation. Therefore, Achromobacter sp. HZ01 may employed as an excellent degrader to develop one cost-effective and eco-friendly method for the bioremediation of marine environments polluted by crude oil. 相似文献