Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

H. Jonsson  K. Viken Sandnes  D. Schiedek  R. Schneider  B. E. Grsvik  A. Goksyr 《Marine environmental research》2004,58(2-5):655

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.  相似文献   

Influence of Current Width Variation on the Annual Mean Transport of the East Sakhalin Current: A Simple Model     

Yoichi?ShimadaEmail author  Atsushi?Kubokawa  Kay?I.?Ohshima 《Journal of Oceanography》2005,61(5):913-920

Recent observations suggest that the annual mean southward transport of the East Sakhalin Current (ESC) is significantly larger than the annual mean Sverdrup transport. Motivated by this observational result, transport of a western boundary current has been investigated using a simple numerical model with a western slope. This transport is defined as the instantaneous barotropic transport integrated from the western boundary to the offshore point where the barotropic velocity vanishes. The model, forced by seasonally varying wind stress, exhibits an annual mean of the western boundary current transport that is larger than that of the Sverdrup transport, as observed. The southward transport from October to March in the model nearly equals the instantaneous Sverdrup transport, while the southward transport from April to September decreases slowly. Although the Sverdrup transport in July vanishes, the southward transport in summer nearly maintains the annual mean Sverdrup transport, because the barotropic Rossby wave cannot intrude on the western slope. This summer transport causes the larger annual mean. Although there are some uncertainties in the estimation of the Sverdrup transport in the Sea of Okhotsk, the seasonal variation of the southward transport in the model is qualitatively similar to the observations.  相似文献   

Study on short-range numerical forecasting of ocean current in the East China Sea——Ⅰ Basic problems of ocean current forecasting and structure of the models     

Zhao Jinping  Chen Zhongyong  Shi Maochong 《海洋学报(英文版)》1993,12(3):335-345

Ocean current forecasting is still in explorative stage of study. In the study, we face some problems that have not been met before. The solving of these problems has become fundamental premise for realizing the ocean current forecasting. In the present paper are discussed in depth the physical essence for such basic problems as the predictability of ocean current, the predictable currents, the dynamical basis for studying respectively the tidal current and circulation, the necessity of boundary model, the models on regions with different scales and their link. The foundations and plans to solve the problems are demonstrated. Finally a set of operational numerical forecasting system for ocean current is proposed.  相似文献   

Deep sea navigation techniques     

K. G. Robertson 《Marine Geophysical Researches》1990,12(1-2):3-8

Accurate navigation forms an essential part of all research at sea and the deep ocean imposes it's own unique problems. This chapter discusses several of the techniques in current use on the research vessels of the Natural Environment Research Council (NERC), concentrating on those systems which provide global navigation facilities, as opposed to the more localised, coastal aids. Whilst most of the systems rely on surface propagation of radio waves, the use of acoustics and sea-bed mapping instruments constitute accurate alternatives for some sub-sea applications.  相似文献   

三维斜压模式对冬季南海环流的数值计算   总被引：7，自引：0，他引：7  

游小宝  李荣凤  张铭  曾庆存 《海洋学报》2001,23(6):1-10

用一个三维、自由表面、原始方程模式对南海环流进行了计算.计算结果表明:黑潮在巴士海峡以西呈一反气旋弯曲流动路径,有一相对高温高盐的水舌从巴士海峡伸入南海,表明有部分黑潮水侵入南海.冬季南海的一些观测事实在模式结果里得到了很好的反映,像冬季逆风向东北方向流动的“南海暖流”和一些中尺度涡旋.同时还分析了巴士海峡沿120.75°E断面的流速和盐度的垂直结构,并同观测结果进行了比较.根据模式结果,我们还进一步讨论了“南海暖流”的形成和驱动机制.  相似文献   

Atmospheric input of trace elements to the western Pacific Ocean and the Kuroshio ocean area     

Qian Fenlan  Yu Hongjian  Lan Youchang  Chen Zhi  Zhou Mingyu  Farn Parungo  Wu Peiming 《海洋学报(英文版)》1996,15(1):69-84

ＡｔｍｏｓｐｈｅｒｉｃｉｎｐｕｔｏｆｔｒａｃｅｅｌｅｍｅｎｔｓｔｏｔｈｅｗｅｓｔｅｒｎＰａｃｉｆｉｃＯｃｅａｎａｎｄｔｈｅＫｕｒｏｓｈｉｏｏｃｅａｎａｒｅａ￥ＱｉａｎＦｅｎｌａｎ；ＹｕＨｏｎｇｊｉａｎ；ＬａｎＹｏｕｃｈａｎｇ；ＣｈｅｎＺｈｉ；Ｚｈｏｕ...  相似文献   

黄、东海环流的数值研究Ⅲ——正压环流的数值模拟     

刘桂梅  孙文心  雷坤  江文胜 《中国海洋大学学报(自然科学版)》2002,32(1):1-8

依据黄、东海环流的的动力学模型 ,运用“流速分解法”对黄、东海正压环流进行了数值模拟。计算结果表明冬季黄海正压环流主要受风应力影响 ,基本形态为黄海暖流由济州岛西南进入南黄海中部 ,其东西两侧分别为两支向南流动的沿岸流 ;夏季主要受到潮致体力的影响 ,为一逆时针涡旋。东海环流主要是边界力作用驱动的结果 ,东海黑潮、台湾暖流和对马暖流较稳定。冬季风应力对东海环流表层流场有消弱作用 ,在夏季则有一定增强作用。  相似文献   

闽江河口潮流数值模拟     

梁金焰 《台湾海峡》1997,16(1):1-9

应用边界拟合坐标系统，解决了闽江河口河道曲折汊道多造成的计算方向与河底走向交角过大的难点；针对闽江河口长宽比相差较大的特点，移植了通常用于河道计算的沿纵向求解大尺度矩阵问题转化为沿横向小尺度矩阵求逆的水流方程求解方法，采用全隐差分格式，使闽江口数模网格步长最小控制在50m左右，时步长达3min，潮位、流场与实测拟合良好。  相似文献   

Impact of Two "El Niño" Events of Different Intensity on the Hypoxic Soft Bottom Macrobenthos off the Central Peruvian wast     

Juan  Tarazona  Wolf E.  Arntz Elba  Canahuire 《Marine Ecology》1996,17(1-3):425-446

Abstract. Monthly changes in the community structure of hypoxic soft-bottom macrobenthos have been studied at a station at 34 m depth in Ancón Bay (Peru) before and during two El Niño (EN) events. Of these events, 1982-83 is considered the strongest, and 1991–93 one of the most prolonged in the 20th century. On the oceanographic scale, EN 1982–83 ranges as "very strong", whereas EN 1991–93 ranges as "moderate".
The thermal anomalies at the station during EN 1982–83 (+ 7.8 °C) were almost twice those of EN 1991–93 (+ 4.1 °C). However, the community changes were not in all cases proportional to the extent of warming. Species numbers increased in a similar way (up to 24 species from near zero in each of the two events), but maximum faunal density was five times higher, and mean biomass was twice as high in 1982–83 compared with the event a decade later. Species diversity was slightly higher during EN 1982–83, whereas successional and trophic changes occurred on a broader scale during EN 1991–93. On the whole, the impact of the long-lasting event on the small soft-bottom macrofauna was not much weaker than that of the exceptionally strong event.
The authors discuss the mechanisms which may be responsible for the differences and similarities encountered in the benthic community dynamics during these two events. Both in the unusually strong and the unusually long EN, the community revealed a pattern of early biological response several months ahead of the onset of local warming which marks the official begin of EN. The question is addressed to what extent the increase of certain parameters in the benthic community could be used for predictive purposes.  相似文献   

The Formation and Circulation of the Intermediate Water in the Japan Sea   总被引：1，自引：0，他引：1  

Jong-Hwan Yoon  Hideyuki Kawamura 《Journal of Oceanography》2002,58(1):197-211

In order to clarify the formation and circulation of the Japan/East Sea Intermediate Water (JESIW) and the Upper portion of the Japan Sea Proper Water (UJSPW), numerical experiments have been carried out using a 3-D ocean circulation model. The UJSPW is formed in the region southeast off Vladivostok between 41°N and 42°N west of 136°E. Taking the coastal orography near Vladivostok into account, the formation of the UJSPW results from the deep water convection in winter which is generated by the orchestration of fresh water supplied from the Amur River and saline water from the Tsushima Warm Current under very cold conditions. The UJSPW formed is advected by the current at depth near the bottom of the convection and penetrates into the layer below the JESIW. The origin of the JESIW is the low salinity coastal water along the Russian coast originated by the fresh water from the Amur River. The coastal low salinity water is advected by the current system in the northwestern Japan Sea and penetrates into the subsurface below the Tsushima Warm Current region forming a subsurface salinity minimum layer. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献