Elevational patterns of abundant and rare bacterial diversity and composition in mountain streams in the southeast of the Tibetan Plateau     

Keshao LIU  TANDong YAO  Yongqin LIU  Baiqing XU  Anyi HU  Yuying CHEN 《中国科学:地球科学(英文版)》2019,(5):853-862

Elevational gradients are powerful ‘natural laboratory' for testing the responses of microbes to geophysical influences. Microbial communities are normally composed of a few abundant and many rare taxa. Abundant and rare taxa play different ecological roles in kinds of environments, but how their diversity and composition patterns response to elevation gradients is still poorly elucidated. In this study, we investigated the elevational patterns of abundant and rare bacterial diversity and composition in a mountain stream from 712 to 3435 m at Gangrigabu Mountain on the Tibetan Plateau, China. Our results revealed abundant and rare bacteria had similar decreasing elevation trend of alpha diversity, and both of them showed a significant elevational distance-decay relationship. However, the turnover rate of the elevational distance-decay of rare bacteria was higher than that of abundant bacteria. The species-abundance distribution patterns of rare taxonomic composition were associated with the elevational gradient, while most of abundant bacterial clades did not display any relationships with elevation.Our results suggested that rare bacteria were more sensitive to changes in elevation gradient.  相似文献   

Can be the environmental and biotic factors responsible for macroinvertebrate communities’ alterations in Portuguese alpine ponds?     

《Limnologica》2020

Alpine ponds are shallow waterbodies formed in alpine zones characterized by usual pristine conditions, however subject to harsh conditions that enable the emergence of unique communities with high conservational value. Therefore, it is important to gather information about these ecosystems and understand the changes in the biota. This work aimed to use Portugal’s alpine ponds as a case of study in assessed the macroinvertebrates communities in the Mediterranean ecozone. To fulfill this goal, we aimed to understand the alterations on macroinvertebrates community and how are they modulated by environmental variables. Five alpine ponds in Serra da Estrela (Portugal) were studied for one year. Macroinvertebrate communities and physical and chemical water parameters were determined in June/16, October/16, and April/17. Water chemistry values were similar to other studied lentic waterbodies in alpine zones in Europe, with slightly acidic water and low conductivity. Although accentuated macroinvertebrates differences between ponds, no significant differences in diversity were recorded, indeed the main differences recorded were influenced by ponds habitat types and environmental variables. Canonical Correspondence Analysis showed differences in the communities between ponds, namely the high influence of several physical and chemical parameters (conductivity, phosphate content, and pH) in the macroinvertebrates community composition. Indeed, Spearman’s correlation revealed significant interaction in diversity, abundance and richness of macroinvertebrates and several physical and chemical parameters (pH, dissolved oxygen and nitrates concentration).  相似文献   

Abschätzung der Eignung einer molekularbiologischen Methode zur Charakterisierung der Grundwasserbiozönose     

B. Kuhlmann  B. Kilb  G. Preuß 《洁净——土壤、空气、水》2000,28(5):250-255

Assessing the Suitability of a Molecularbiological Method To Characterise the Microbial Populations in Groundwater A molecularbiological technique was used to characterise the bacterial community structure of groundwater habitats. This method consists of the isolation of bacterial DNA from the samples, amplification of 16S rDNA by PCR (polymerase chain reaction), and separation of the amplified DNA by DGGE (denaturing gradient gel electrophoresis). By using more specific primer combinations in the PCR instead of universal eubacterial primers, also groups of microorganisms (Proteobacteria, sulfate reducer, Archaea) were determined. The resulting DGGE patterns that reflect the microbial diversity are compared and differences or similarities evaluated. In the present studies, groundwater from different sites (bank filtrate, artificially recharged groundwater, and natural groundwater) and with changing redox milieus (aerobic, anaerobic) were investigated as well as the solid aquifer material. Besides, samples were taken from the different stages of artificial groundwater recharge, i.e., from surface water to the drain tile. Samples from groundwater derived from sites with different hydrogeochemical or hydrological conditions like bank filtrate and recharged groundwater revealed great differences in DGGE patterns indicating a characteristic species composition in these habitats, while samples taken at different times from the same groundwater showed only small seasonal variations. Clearly different patterns were also found for groundwater and the adjacent solid material as well as for anaerobic and aerobic groundwaters. Looking at artificial groundwater recharge, almost identical patterns were found in raw water and samples from gravel and sand filtration. DGGE patterns from the resulting groundwater indicated a total change in community structure during underground passage. By using group specific primers, Desulfovibrionaceae, Desulfobacteriaceae, and Archaea could be detected in anaerobic groundwaters.The molecularbiological approach described here gives an increasingly comprehensive and more precise picture of the microbial population of different environments. It is especially suitable to compare the community structure from different habitats or to analyse changes for example due to environmental stress at the same site.  相似文献   

Local and regional species diversity of benthic Isopoda (Crustacea) in the deep Gulf of Mexico     

George D.F. Wilson   《Deep Sea Research Part II: Topical Studies in Oceanography》2008,55(24-26):2634

Recent studies of deep-sea faunas considered the influence of mid-domain models in the distribution of species diversity and richness with depth. In this paper, I show that separating local diversity from regional species richness in benthic isopods clarifies mid-domain effects in the distribution of isopods in the Gulf of Mexico. Deviations from the randomised implied species ranges can be informative to understanding general patterns within the Gulf of Mexico. The isopods from the GoMB study contained 135 species, with a total of 156 species including those from an earlier study. More than 60 species may be new to science. Most families of deep-sea isopods (suborder Asellota) were present, although some were extremely rare. The isopod family Desmosomatidae dominated the samples, and one species of Macrostylis (Macrostylidae) was found in many samples. Species richness for samples pooled within sites ranged from 1 to 52 species. Because species in pooled samples were highly correlated with individuals, species diversity was compared across sites using the expected species estimator (n=15 individuals, ES₁₅). Six depth transects had idiosyncratic patterns of ES₁₅, and transects with the greatest short-range variation in topography, such as basins and canyons, had the greatest short-range disparity. Basins on the deep slope did not have a consistent influence (i.e., relatively higher or lower than surrounding areas) on the comparative species diversity. ES₁₅ of all transects together showed a weak mid-domain effect, peaking around 1200–1500 m, with low values at the shallowest and deepest samples (Sigsbee Abyssal Plain); no longitudinal (east–west) pattern was found. The regional species pool was analyzed by summing the implied ranges of all species. The species ranges in aggregate did not have significant patterns across longitudes, and many species had broad depth ranges, suggesting that the isopod fauna of the Gulf of Mexico is well dispersed. The summed ranges, as expected, had strong mid-domain patterns, contrasting with the local species richness estimates. The longitudinal ranges closely matched a randomized pattern (species ranges placed randomly, 1000 iterations), with significant deviations in the east attributable to lower sampling effort. The depth pattern, however, deviated from the mid-domain model, with a bimodal peak displaced nearly 500 m shallower than the mode of the randomized distribution. The deviations from random expectation were significantly positive above 1600 m and negative below 2000 m, with the result that regional species richness peaked between 800 and 1200 m, and decreased rapidly at deeper depths. The highest species richness intervals corresponded to the number of individuals collected. Residuals from a regression of the deviations on individual numbers, however, still deviated from the randomized pattern. In this declining depth-diversity pattern, the Gulf of Mexico resembles other partially enclosed basins, such as the Norwegian Sea, known to have suffered geologically recent extinction events. This displaced diversity pattern and broad depth ranges implicate ongoing re-colonization of the deeper parts of the Gulf of Mexico. The Sigsbee Abyssal Plain sites could be depauperate for historical reasons (e.g., one or more extinction events) rather than ongoing ecological reasons (e.g., low food supply).  相似文献   

Altitude and adaptation: A study of geography and ethnic division     

《Applied geography (Sevenoaks, England)》2013

In this paper we investigate the influence of geography on ethnic division by drawing evidence from Tibetan ethnic regions in China. Using a novel township name index that identifies the ethnolinguistic origin of each township name, our empirical findings first show that regions with Han Chinese settlement in the past also witness higher concentration of Han population today. We also show that townships located at higher altitudes have less Han concentration. This altitude effect can be both indirect and direct; we find that the indirect effect through historical settlement is small compared to the direct physiological effect through altitude illness. The challenging environment of the plateau region acts as a physiological hindrance only for Han Chinese, and the natural separation continues to persist between the two groups.  相似文献   

曼氏无针乌贼（Sepiellamaindroni）ISSR体系优化及养殖群体遗传多样性分析     

徐梅英  叶莹莹  郭宝英  祁鹏志  吴常文 《海洋与湖沼》2011,42(4):538-542

采用ISSR-PCR技术对曼氏无针乌贼基因组DNA进行扩增,筛选分析了ISSR—PCR扩增时的主要影响因子,包括Mg^2＋浓度、引物浓度、dNTPs浓度、Taq酶浓度以及退火温度,并用8条ISSR引物对舟山养殖群体进行了遗传多样性分析,最终确立的适用于曼氏无针乌贼ISSR扩增的25μl反应体系为：Mg2＋1．5mmol...  相似文献   

鄱阳湖荷溪退田还湖湿地区域鸟类多样性及其保护对策     

张敬皆 《湿地科学与管理》2011,7(4):22-24

调查了鄱阳湖荷溪区域湿地鸟类资源,并对164种鸟类的种类组成、区系特点、珍稀濒危性、鸟类季节性、生态分布进行了分析,针对湿地鸟类保护中存在的问题,提出了保护对策。  相似文献   

Net primary productivity and species diversity of herbaceous vegetation in banj-oak (Quercus leucotrichophora A. Camus) forest in Kumaun Himalaya, India     

Mukesh JOSHI  Y.S.RAWAT 《山地科学学报》2011,8(6):787-793

Net primary productivity and species diversity of herbaceous vegetation of banj-oak (Quercus leucotrichophora A.Camus) forest in Kumaun Himalaya,India were analyzed.Across different growth forms (tall forbs,short forbs,cushion and spreading forbs,grasses),short forbs were most dominant component during rainy season (IVI=152) and winter season (IVI=167) and grasses during the winter season (IVI=148).Maximum above-ground production occurred during rainy season (132.5 g m-2) and minimum during winter season (2.8 g m-2).Below-ground production was maximum (85.9 g m-2) during winter season and minimum (14.9 g m-2) during summer season.Annual net shoot production was 150 g m-2 and below-ground production was 138 g m-2.Of the total input 61 % was channeled to above-ground parts and 39% to below-ground parts.  相似文献   

辽宁营口大辽河口水鸟多样性及季节动态     

曾娅杰  贾亦飞 《湿地科学与管理》2022,18(1):18-22

营口市大辽河口湿地是东亚-澳大利西亚迁飞区水鸟的重要栖息地。2019年,采用样线法和定点观察法,对大辽河口湿地水鸟进行监测,运用指数分析方法研究湿地水鸟多样性。结果表明:共记录到湿地水鸟5目10科45种230355只,其中,鸻形目29种,国家Ⅰ级保护动物2种,国家Ⅱ级保护动物5种,IUCN濒危物种2种,易危物种2种,近危物种2种,个体数量超过全球数量1%标准的有13种。从居留型来看,以旅鸟为主,其个体数量占水鸟个体总数的90.06%。大辽河口湿地水鸟多样性季节差异明显,春季的水鸟个体数量最高,8月的水鸟多样性指数最高,3月的水鸟均匀度指数最高。营口大辽河口湿地水鸟多样性高,种群数量大,已达到国际重要湿地的标准,应尽快纳入国家湿地保护体系。  相似文献   

Macrobenthic associations in a South Atlantic Brazilian enclosed bay: the historical influence of shrimp trawling     

de Almeida TC  Vivan JM 《Marine pollution bulletin》2011,62(10):2190-2198

This study characterized the macrobenthos of Tijucas Bay and related the distribution and dominance patterns with historical trawling activities. Fifteen sampling sites were established in the bay and were sampled in summer and winter for the characterization of the macrobenthos. To evaluate the trawling effects, three passages were established at each site with control and impacted areas analyzed before and after trawl-net action in impacted area. Seasonal differences occurred in macrobenthos, possibly influenced by rainfall where the relative water level of the Tijucas River was directly associated with the discharge of freshwater and particulate matter. The associations identified in the bay were distinct in comparison with the Zimbros Embayment. The trawling assessment showed no significant variation in the macrobenthos after the trawl, possibly due to decades of shrimp exploitation in the bay. Trawling and instable environmental conditions inside the bay make the establishment of a stable macrobenthic community difficult.  相似文献