海带雌性原生质体的分离与培养     

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(S1):126-129

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。  相似文献   

Distribution of As, Cr and V species in the Po-Adriatic mixing area, (Italy)   总被引：1，自引：0，他引：1  

M. Pettine  D. Mastroianni  M. Camusso  L. Guzzi  W. Martinotti 《Marine Chemistry》1997,58(3-4)

The distribution of dissolved and participate concentrations of As, Cr and V was investigated in the mixing area of the Po river in five surveys over the period March 1992-March 1995. The data obtained indicate noticeable contamination of coastal waters of the northern Adriatic with Cr but not As or V. Total dissolved concentrations for As and V were comparable with oceanic levels but were higher by a factor of 4 for Cr. These results suggest either low anthropogenic inputs of As and V, from terrigenous and atmospheric sources, or efficient removal mechanisms. The investigated metals had low reactivity during freshwater-seawater mixing and the distribution of concentrations in the dissolved and solid phases suggests that the dilution process is the main factor controlling the transport of these metals to the sea in low-to-medium solid transport conditions. Further investigations are required to elucidate the behavior of these metals during high solid transport conditions. Significant changes in the speciation of As occurred during the transition between the two end-member matrices. In the coastal waters of the northern Adriatic, the arsenite fraction in winter, and the arsenite and dimethylarsinate fractions in summer increased with respect to riverine waters. Changes in the speciation of chromium were minor, but also showed a small increase in reduced species in marine waters.  相似文献   

Determination of technical efficiency of fisheries by stochastic frontier models: a case on the Gulf of Cadiz (Spain)     

Garcia del Hoyo  Juan Jose; Castilla Espino  David; Jimenez Toribio  Ramon 《ICES Journal of Marine Science》2004,61(3):416-421

  相似文献   

基于矢量信号处理的水声定位系统   总被引：2，自引：1，他引：2  

宋新见殷冬梅  惠俊英 《海洋工程》2003,21(3):110-114

将传统的水声定位系统与矢量水听器相结合，设计了一种全新的轻便型长基线被动水声定位系统。介绍了系统的组成和工作原理，并结合近年来出现的矢量信号处理技术，设计了新的实时信号处理软件。经湖试和海试，系统的可行性得到了初步的验证。  相似文献   

A simple method for comparing age-length keys reveals significant regional differences within a single stock of haddock (Melanogrammus aeglefinus)     

Gerritsen  Hans D.; McGrath  David; Lordan  Colm 《ICES Journal of Marine Science》2006,63(6):1096-1100

  相似文献   

福建闽南沿海养殖贝类体中砷含量的分布   总被引：9，自引：0，他引：9  

钟硕良 《海洋学报》2005,27(6):116-122

通过2003~2004年对福建闽南沿海5种养殖贝类体砷含量的调查监测,对养殖贝类体中砷含量的分布进行了探讨.福建闽南沿海5种养殖贝类体总砷含量为0.65×10-6~4.80×10-6,总平均值为2.09×10-6,其中有机砷和无机砷占总砷含量的百分比分别为61.6%~98.2%和2.3%~38.4%,平均值分别为88.8%和11.2%.在相同养殖区,养殖贝类体总砷平均含量的种间分布差异较小.滩涂养殖底栖贝类体内总砷平均含量总体上高于浅海筏式养殖贝类体的平均含量.福建闽南沿海5种养殖贝类体内总砷含量在泉州湾、湄州湾、东山湾和九龙江口高,在诏安湾和围头湾低,在其余各湾相近.5种养殖贝类体内无机砷的污染指数和总平均值分别为0.05~0.76和0.23,总砷的污染指数和总平均值分别为0.06~0.48和0.21,总体符合国家食品卫生质量标准.养殖贝类体中砷含量与体长之间总体成正相关.  相似文献   

Modelling the relationship between sexual reproduction and rhizome growth in Posidonia oceanica (L.) Delile     

Sebastiano Calvo  Gianfranco Lovison  Maria Pirrotta  Germana Di Maida  Agostino Tomasello  & Mariangela Sciandra 《Marine Ecology》2006,27(4):361-371

The relationship between flowering and growth performance of Posidonia oceanica (L.) Delile in meadows distributed along the south‐eastern coast of Sicily (Italy) was investigated by means of a statistical model (generalized linear mixed model) combined with the lepidochronological analysis. Over a 28‐year period, 67 floral stalk remains were observed. The highest flowering index was recorded in lepidochronological year 1998 (10.1%) and the Inflorescence Frequency per age showed a clear decrease corresponding to 15‐year‐old shoots. The sexual reproductive event had positive effects on rhizome elongation (cm year^?1) and leaf production (no. leaves year^?1) in the same flowering year, whilst no effect on the rhizome production (mg year^?1) was observed. Rhizome growth variables showed significant negative lagged responses in the two years following flowering. On the whole, we calculated that the effect exerted by flowering, in terms of loss on rhizome elongation and production, was about 27% and 38%, respectively. Although it has been demonstrated that recovery from the stress induced by sexual reproduction is limited to the two years after flowering, the magnitude of the reproductive cost may become quite considerable especially in comparison with the whole lifespan of individual shoots.  相似文献   

Analysis of non-linear relationships between catch per unit effort and abundance in a tuna purse-seine fishery simulated with artificial neural networks   总被引：3，自引：0，他引：3  

Gaertner  Daniel; Dreyfus-Leon  Michel 《ICES Journal of Marine Science》2004,61(5):812-820

  相似文献   

Influence of Rhodolith-Forming Species and Growth-Form on Associated Fauna of Rhodolith Beds in the Central-West Gulf of California, México     

Gustavo Hinojosa-Arango  & Rafael Riosmena-Rodríguez 《Marine Ecology》2004,25(2):109-127

Abstract.  Rhodoliths provide a stable and three-dimensional habitat to which other seaweeds and invertebrates can attach. Although ecological factors affecting rhodolith beds have been studied, little is known about the effect of rhodolith species and growth-form on associated fauna. Experiments were conducted at three rhodolith beds in the central-west Gulf of California. Faunal abundance differed significantly in relation to rhodolith-forming species, but no significant differences were observed between different growth-forms. Rhodolith structure differs between the species Lithophyllum margaritae and Neogoniolithon trichotomum , and the combination of structure differences and rhodolith abundances may be responsible of the significant differences in faunal abundance and richness. Crustaceans, polychaetes and molluscs were the most important taxa in all three rhodolith beds. The amphipod species Pontogeneia nasa and the cnidarian Aiptasia sp. were dominant in both rhodolith beds, El Requesón and Isla Coyote, in Bahía Concepción. The Isla Coronados rhodolith bed was dominated by an unidentified harpacticoid copepod (Copepoda sp.1). Rhodolith species is more important than growth-form in determining abundance and richness of the associated fauna. Nevertheless, factors such as wave motion, depth, bioturbation and others should be considered when studying organisms associated with rhodolith beds.  相似文献   

Reproductive biology of scad, Trachurus mediterraneus (Teleostei, Carangidae), from the Gulf of Trieste     

Viette  Michele; Giulianini  Piero Giulio; Ferrero  Enrico Antonio 《ICES Journal of Marine Science》1997,54(2):267-272

  相似文献