深圳杨梅坑海域造礁石珊瑚共生藻分类和遗传多样性研究          下载免费PDF全文

陈文静  佟飞  陈丕茂  朱爱意  张露  李丹丹  龙鑫玲 《应用海洋学学报》2019,38(2):284-291

通过核糖体ITS(Internal Transcribed Spacer)的核苷酸序列研究了深圳杨梅坑海域20种47个造礁石珊瑚样本的共生藻。通过ITS序列分析,与GenBank上的4种不同的共生藻构建Neighbor-Joining聚类树,进行石珊瑚共生藻分类和遗传多样性分析。结果表明,该海域的造礁石珊瑚共生藻属于2种不同的种类(亚系群),19个样品属于C1亚系群共生藻,1个样品属于C15亚系群共生藻,C1和C15两个亚系群共生藻之间的遗传距离为0. 01。将深圳杨梅坑海域得到的ITS序列与NCBI数据库上的福建东山海域、广东徐闻地区的C1系共生藻进行比对,只有深圳杨梅坑海域藻类样本平均(A+T)碱基含量为49. 4%,(A+T)含量小于(G+C)含量。构建Neighbor-Joining聚类树表明,深圳杨梅坑海域石珊瑚共生藻与福建东山海域的亲缘关系较近,而与广东徐闻地区的亲缘关系较远,地理隔离是主要的因素。  相似文献   

河道洪水演进数值模拟及其在GIS平台上的可视化   总被引：1，自引：0，他引：1  

吴迪军  孙海燕  黄全义  李静兰 《测绘科学》2009,34(6)

针对公共安全应急平台中灾害预测预警和应急处置的快速反应需求,建立了基于圣维南方程的河道洪水演进的水动力学模型。采用库朗格式的特征线方法对基本方程进行数值求解,得出水位和流量沿河道的时空变化关系,在此基础上进一步研究了基于GIS的洪水演进可视化方法。最后,通过天然河道模拟实例验证了所建模型的可靠性和实用性。  相似文献   

Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>     

王昌留  张士璀  宿烽  王磊  李红岩 《中国海洋湖沼学报》2009,27(1):69-73

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)  相似文献   

In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)     

WANG Yinan  ZHAN Wenbin  XING Jing  JIANG Yousheng 《海洋学报(英文版)》2008,27(2):126-132

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.  相似文献   

轨道平顺性与测量精度   总被引：1，自引：0，他引：1  

葛忠土 《地理空间信息》2009,7(4):133-136

从轨道平顺性验收标准性切入，提出轨道平顺性对控制网的精度要求；指出轨道平顺性与控制点间的相对精度相关，与控制测量网垂直于线路的的横向误差及高程相关。  相似文献   

Distribution characteristics of heavy metals in surface sediments and core sediments of the Shenzhen Bay in Guangdong Province, China   总被引：1，自引：1，他引：0  

ZUO Ping  WANG Yaping  MIN Fengyang  CHENG Jun 《海洋学报(英文版)》2009,28(6):53-60

The concentration of zinc, chromium, cadmium, nickel, lead and copper was measured on both surface sediments and core sediments in the Shenzhen Bay. The average contents of all metals have exceeded the background values in the coastal zone of Guangdong Province. Cadmium is a heavy pollution matter which indicates a strong pollution level according to the ecological risk coefficient method. The ecological risk index of the whole area is 299 which belongs to middle and strong level. The area with strong pollution level is the near shore area closing to the inside of Shenzhen Bay, Shakou area is in the middle level while the weak pollution level occurs in Shenzhenhe Estuary according to ecological risk index. The four sediment cores can be divided into two parts based on lead-210 dating, sediments size analysis and economic development period in surrounding areas since 1950 with strong human intervention. The sediment size, deposition rate and human activities have positive relationship with the contents of heavy metals which directly impact the pollution level of heavy metals in sediments in Shenzhen Bay.  相似文献   

济南张夏组灰岩岩溶地下水供水潜力探讨   总被引：1，自引：1，他引：0       下载免费PDF全文

韩祥银  韩亮  付春升  方庆海  王集宁  刘广哲 《山东国土资源》2008,24(12)

济南南部广布巨厚张夏组灰岩,其中赋存优质丰富的岩溶地下水。出露众多泉水,其流量为800～8000m3/d。该文通过简述济南地区张夏组灰岩岩溶地下水的赋存条件与水文地质特征,概略计算与评价其资源量,论证开发利用这一新水源的可行性,以期为济南供水与保泉开辟新途径。  相似文献   

离散位场数据格网化方法探讨   总被引：1，自引：0，他引：1  

方杨 《测绘信息与工程》2009,34(6):46-48

针对均衡异常数据,比较分析了连续曲率张力样条方法、最小曲率方法和Shepard方法,结果表明张力样条方法解的全局效果较好。  相似文献   

低端掌上机在控制测量中的前景分析          下载免费PDF全文

张战伟  马艳艳  易天阳 《山东国土资源》2008,24(7)

论述了开发低端电子手簿的可行性，介绍了HP200LX掌上机的基本特点，实用汉字库的建立原理和使用，软件界面设计的基本原则和具体做法，以及手簿的功能和特点，提出了中文电子手簿设计及开发的思路。  相似文献   

利用南极走航观测评估卫星遥感海表面温度   总被引：3，自引：1，他引：2  

李明  张占海  刘骥平  孙波  吴辉碇  李娜 《海洋学报》2008,30(3):16-27

利用1989-2005年间南极走航观测的海表面温度,对目前3个主要的卫星反演的SST产品AVHRR(Advanced Very High Resolution Radiometer),TMI(TRMM Microwave Imager)和AMSR-E(Advanced Microwave Scanning Radiometer for the Earth Observing System)进行了较为系统的评估,并着重检验了它们在南大洋的准确性.结果表明,AVHRR SST比观测数据偏冷,白天的偏差为-0.12℃,夜晚的偏差为-0.04℃,而且南大洋的冷偏差更为显著.TMI SST比观测数据明显偏暖,白天的偏差为0.48℃,夜晚的偏差为0.57℃,其温差ΔT受37GHz风速影响,在强风速(>6m/s)下这种影响仍然存在.AMSR-ESST比观测数据偏暖,白天的偏差为0.34℃,夜晚的偏差为0.27℃,而且南大洋的暖偏差相对较大.AMSR-E SST温差受水汽影响,并在南大洋随着水汽的增加而增加.通过进一步比较微波(AMSR-E和TMI)和红外(AVHRR)遥感的SST在2004年北半球冬季(即南半球夏季)的差别,发现微波遥感在热带(15°S-15°N)和南大洋区域(45°S以南)比红外遥感偏暖,而且在南大洋区域的偏差相对较大,相反在北半球中纬度区域(15°~40°N)偏冷.AMSR-E与AVHRR SST的温差,从白天到夜晚有减小的趋势,而TMI与AVHRR SST的温差无明显的变化.  相似文献