假单胞菌(Pseudomonas sp.cn4902)磷酸甘油磷酸酯酶基因克隆与鉴定     

刘广发  张会永  谢金镇 《海洋与湖沼》2005,36(4):313-318

采用随机克隆、功能筛选、逐次排除和同源比较的基因克隆新策略进行克隆假单胞菌(Pseudomonas sp．cn4902)磷酸甘油磷酸酯酶基因的研究。结果表明，该结构基因长819bp，与铜绿假单胞菌的磷酸甘油磷酸酯酶基因的核苷酸一致性达61．5％，氨基酸同源性为56．2％。该基因已输入GenBank数据库，收录号AF348165。将该基因转化大肠杆菌，受体菌在含NaCl 1．0mol／L的培养基中甘油含量升高2．9倍，最终菌浓度提高3．6倍。可见这是一个与生物耐盐性相关的主基因，以其转化、培育耐盐农作物的前景十分光明。  相似文献   

Depth- and current-induced effects on wave propagation into the Altamaha River Estuary, Georgia     

KiRyong Kang  Daniela Di Iorio   《Estuarine, Coastal and Shelf Science》2006,66(3-4):395-408

A study of sea surface wave propagation and its energy deformation was carried out using field observations and numerical experiments over a region spanning the midshelf of the South Atlantic Bight (SAB) to the Altamaha River Estuary, GA. Wave heights on the shelf region correlate with the wind observations and directional observations show that most of the wave energy is incident from the easterly direction. Comparing midshelf and inner shelf wave heights during a time when there was no wind and hence no wave development led to an estimation of wave energy dissipation due to bottom friction with corresponding wave dissipation factor of 0.07 for the gently sloping continental shelf of the SAB. After interacting with the shoaling region of the Altamaha River, the wave energy within the estuary becomes periodic in time showing wave energy during flood to high water phase of the tide and very little wave energy during ebb to low water. This periodic modulation inside the estuary is a direct result of enhanced depth and current-induced wave breaking that occurs at the ebb shoaling region surrounding the Altamaha River mouth at longitude 81.23°W. Modelling results with STWAVE showed that depth-induced wave breaking is more important during the low water phase of the tide than current-induced wave breaking during the ebb phase of the tide. During the flood to high water phase of the tide, wave energy propagates into the estuary. Measurements of the significant wave height within the estuary showed a maximum wave height difference of 0.4 m between the slack high water (SHW) and slack low water (SLW). In this shallow environment these wave–current interactions lead to an apparent bottom roughness that is increased from typical hydraulic roughness values, leading to an enhanced bottom friction coefficient.  相似文献   

A Numerical Study on the Structure and Dynamics of Turbulence in Oscillatory Bottom Layer Flow over A Flat or Rippled Bed     

白玉川  吴朝安黄涛 《中国海洋工程》2006,20(1):45-60

1 .IntroductionUnder the influence of surface waves ,sandripples often appear on beaches . Whenthe amplitudeof water oscillationis sufficientlylarge ,vortices are formed onthe lee of every sand ripple crest . A-mongthese vortices ,the most important are t…  相似文献   

Sugar and Lipid Components in Sea Foam near Kelp Beds     

Branko  Velimirov 《Marine Ecology》1982,3(2):97-107

Abstract. A component analysis of the sugar and lipid fraction in foam layers around kelp beds on the South African west coast showed the following trends: In the residue of foam drainage of both 12 h and 120 h old foam the sugar fraction is represented by mannose (33–41 %), galactose (4–30%), fucose (3–29%) and glucose (19–26%). With the exception of fucose, the same components could be found in foam filtration. In the lipid fraction the main fatty acids are palmitic (23.3–27.3%), oleic (14.0–5.5%) and timnodonic acid (11.2–9.1%). Selacholeinic acid was only found in 12 h old foam while lignoceric acid could not be located in foam filtration. Against expectation mannitol was not identified in quantifiable amounts in foam samples although it is the dominant component of kelp mucilage, whose presence is of major importance for foam formation. Hypotheses explaining the lack of mannitol are forwarded. Component sugar and lipid content for 11 of foam was calculated and estimation of sugar and lipid content in an average foam patch (volume of 30001) is given. The importance of foam formation as a mechanism for binding and transfer of energy in the marine environment is emphasized.  相似文献   

水面皮层破坏-复原过程的实验室模拟研究     

吴永森  吴隆业 《海洋与湖沼》2001,32(6):605-611

依据水面红外发射和红外遥感测温原理，采用HDG－高灵敏度红外测温仪和常规测量仪器相结合的方法，在实验室空气稳定条件下，模拟测得了水面皮层破坏－复原（重建）的热力过程和气－水温差对水面皮层复原过程的影响，获得了大量的测量数据。数据分析表明，当气－水温差从3．0℃变为11．5℃时，水面皮层破坏可导致皮温增量从气－水温差3．0℃时的0．15℃变到11．5℃时的0．45℃，并发现恢复时间与气－水温差呈负线性关系。  相似文献   

The impact of salt water inflows on the distribution of polycyclic aromatic hydrocarbons in the deep water of the Baltic Sea     

Gesine Witt  Wolfgang Matthus 《Marine Chemistry》2001,74(4):246

Salt-water inflows into the Baltic Sea are important events for renewing the deep and bottom waters of the deep basins of the Baltic Sea. These events occur only at irregular intervals. The last strong event was in January 1993 followed by minor inflows in winter 1993/1994. As a result of these inflows, the deep water of the central Baltic basins was completely renewed.Based on extensive observations of polycyclic aromatic hydrocarbons (PAHs) in water, fluffy layer material and surface sediments between 1992 and 1998, the transformation of PAHs and the modification of their distribution in the Baltic deep water is discussed in connection with the spreading of the inflowing highly saline and oxygen-rich water along its pathway from the sills into the central basins. In the course of the inflows in 1993/1994, the PAH concentration in the deep water of the different basins increased significantly. The concentrations were elevated, at least by a factor of 2 and as much as seven to eight times (for the four-ring PAHs) compared to the previous and the following years. Two hypotheses for the causes were discussed: the inflowing salt water may have entrained more highly polluted surface water in the western Baltic Sea, or it may have entrained contaminated fluffy layer material or sediment particles along the route of transport.  相似文献   

Full-range equation for wave boundary layer thickness     

Ahmad Sana  Hitoshi Tanaka 《Coastal Engineering》2007

Full-range equation covering all the flow regimes in a wave boundary layer is proposed for the boundary layer thickness. The results are compared with the available experimental data and good agreement has been found. In case of wave boundary layers, there are three definitions of boundary layer thickness in use. Therefore, the full-range equation is derived for three of the definitions. The findings of this study may be useful in calculating suspended sediment transport in coastal environments and studying wave–current combined motion.  相似文献   

Nature of the connection between the Northern and Southern Zechstein Basins across the Mid North Sea High     

M.K. Jenyon  P.M. Cresswell  J.C.M. Taylor 《Marine and Petroleum Geology》1984,1(4):355-363

The seismic expression of a salt-filled channel which cuts across the Mid North Sea High in Quadrant 37 is described, with features interpreted as being produced by salt-edge dissolution forming both eastern and western margins of the channel. The apparent half-graben nature of the channel is shown to be only superficial, and due to complex faulting associated with, but not defining, its western margin. The shallower faulting here is a Mesozoic to early Tertiary growth fault related to local dissolution of Zechstein salt. The dissolution effect appears in turn to have been localized by the presence of a deeper fault that was already downthrown to the east in Zechstein times, when it seems to have limited the eastward progradation of Zechstein shelf carbonates and anhydrites, and had probably ceased to move significantly before the onset of the Late Cimmerian erosional phase. The origin of this arcuate fault is tentatively ascribed to subsidence around a granite batholith. Zechstein salt originally spread some distance to the east and west of the channel; it was dissolved from the edges inwards, mainly before the Late Cretaceous, giving rise to a thicker Mesozoic sequence on parts of the flanks of the channel than in the middle. Besides providing an interesting structural case history, the features described have implications regarding Zechstein sedimentation, reservoir potential, the tectonic history of the North Sea, and the nature of the Mid North Sea High itself.  相似文献   

中国北方盐田和盐湖高盐水域藻类的初步研究   总被引：3，自引：0，他引：3  

马志珍 《海洋与湖沼》1995,26(3):317-322

于１９８６年３月－１９９０年９月，对中国北方一些盐田和盐湖的高盐水域（盐度６１．０－３２０．０）中的藻类（主要是浮游藻）进行调查。在青岛东风盐场和天津汉沽盐场每月２次定点采集藻类标本水样７２份以及随机在山西解地、青海尕海，新疆达板城盐湖和巴里坤湖采集水样５份。鉴定出蓝藻５种，绿藻８种，硅藻８种。结果表明，均是典型的耐盐或喜盐种类，但其种的多样与水体的盐度呈负相关，藻类种群生物量以春季和秋季最为丰富  相似文献   

0220号热带风暴(米克拉)特征分析     

李孟植 《海洋预报》2003,20(3):7-11

南海是热带气旋活动较为频繁的地区之一。该地区因其独特的地理环境和复杂的热带大气环流系统，在热带气旋预报工作上存在一定的困难。本文在分析一些气象图表资料的基础上，对2002年第20号热带风暴(米克拉)的形成、发展及消亡进行了探讨和研究。主要从形成的背景环流，气旋的结构等方面入手，总结出一些该气旋所具备韵特有流场，从而为以后的南海自生热带气旋预报工作积累一定经验。  相似文献