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151.
S. W. A. Naqvi T. Yoshinari J. A. Brandes A. H. Devol D. A. Jayakumar P. V. Narvekar M. A. Altabet L. A. Codispoti 《Journal of Earth System Science》1998,107(4):367-378
Measurements of15N/14N in dissolved molecular nitrogen (N2), nitrate (NO
3
−
) and nitrous oxide (N2O) and18O/16O in N2O [expressed as δ15N and δ18O, relative to atmospheric N2 and oxygen (O2), respectively] have been made in water column at several locations in the Arabian Sea, a region with one of the thickest
and most intense O2 minima observed in the open ocean. Microbially-mediated reduction of NO
3
−
to N2 (denitrification) in the oxygen minimum zone (OMZ) appears to greatly affect the natural isotopic abundances. The δ15N of NO
3
−
increases from 6‰ in deep waters (2500 m) to 15‰ within the core of the denitrifying layer (250–350 m); the δ15N of N2 concurrently decreases from 0.6‰ to 0.20‰ Values of the isotopic fractionation factor (ε) during denitrification estimated
using simple advection-reaction and diffusion-reaction models are 22‰ and 25‰, respectively. A strong decrease in δ15N of NO
3
−
is observed from ∼ 200m (> 11‰) to 80m (∼ 6‰); this is attributed to the input of isotopically light nitrogen through nitrogen
fixation. Isotopic analysis of N2O reveals extremely large enrichments of both15N and18O within the OMZ, presumably due to the preferential reduction of lighter N2O to N2. However, isotopically light N2O is observed to accumulate in high concentrations above the OMZ indicating that the N2O emitted to the atmosphere from this region cannot be very heavy. The isotope data from the intense upwelling zone off the
southwest coast of India, where some of the highest concentrations of N2O ever found at the sea surface are observed, show moderate depletion of15N, but slight enrichment of18O relative to air. These results suggest that the ocean-atmosphere exchange cannot counter inputs of heavier isotopes (particularly18O) associated with the stratospheric back flux, as proposed by previous workers. This calls for additional sources and/or
sinks of N2O in the atmosphere. Also, the N2O isotope data cannot be explained by production through either nitrification or denitrification, suggesting a possible coupling
between the two processes as an important mechanism of N2O production. 相似文献
152.
为获得反硝化脱氮效率较好的菌株,实验从海水螺旋藻培养体系中分离获得一株嗜碱兼性好氧反硝化菌, 通过观察细菌形态以及16S rRNA基因序列的同源性分析, 鉴定该菌株为海杆菌属, 命名为Marinobacter sp. B3。为明确该海杆菌的反硝化性能及氮转化途径, 研究开展了溶解氧(DO), 碳氮摩尔比(C/N), pH和温度等不同单因素对反硝化性能影响实验和氮平衡实验。单因素影响实验结果表明, 当硝酸钾(KNO3)作为唯一氮源, NO3--N的初始浓度为100 mg/L, 盐度32, 振荡速度为150 r/min (初始DO质量浓度是5.6 mg/L), C/N=10, pH=8.0±0.2, 温度为35 °C时, 可获得最大脱氮效果。氮平衡实验结果得出, 在好氧环境下, 有20.11%的NO3--N转化为胞内氮, 5.58 mg/L的NO3--N转化为其他形态(NO2--N、NO4+-N和有机氮), 74.72%转化为N2释放; 厌氧环境下, 有26.65%的NO3--N转化为胞内氮, 72.86%的NO3--N转化为气态产物释放。最终实验结果表明, Marinobactersp. B3在好氧和厌氧条件下, 48 h对NO3--N的去除率分别为99.89%和93.80%, 具有较好的反硝化脱氮能力, 且在好氧条件下NO3--N去除效率更高, 在海水工厂化循环水养殖尾水处理方面具有良好的应用前景。 相似文献
153.
潮滩生态系统中生源要素氮的生物地球化学过程研究综述 总被引:2,自引:1,他引:1
海岸带潮滩生源要素生物地球化学循环过程是国际地圈生物圈计划(IGBP)、海岸带陆海交互作用(LOICZ)研究的重要内容,也是全球变化区域响应研究中的重要组成部分。在过去的10~20年之间,潮滩生源要素氮的生物地球化学循环研究得到了长足的发展。基于此,较为全面、系统地总结和分析了有关潮滩氮营养盐的来源、潮滩氮素的物理、化学和生物迁移转化过程及氮素地球化学循环过程中底栖生物效应等一系列研究成果,并提出了今后潮滩生源要素氮的生物地球化学循环研究重点和发展趋向。 相似文献
154.
本文从细菌生态学角度结合理化因子阐述了中国对虾养成期虾池水体和底泥中异养菌、条件致病菌(几丁质降解菌和弧菌)的数量变动及菌属组成状况,并与未养虾池作了比较分析。结果表明:虾池内水体中异养菌和条件致病菌数量均比未养虾池水体中的高1~2个数量级,底泥中条件致病菌数量略高于未养虾池。虾池内、外水体中的细菌属组成基本一致,均以革兰氏阴性菌为主,而底泥中的革兰氏阳性菌稍占优势。 相似文献
155.
对虾病原菌抑菌药物的研究 总被引:1,自引:0,他引:1
应用药敏纸片法研究了48种化学疗剂对中国对虾红腿病病原菌(包括Vibrio alg-inolyticus,V.campbellii,V.parahaemolyticus,Proteus vulgaris及未确定的海洋弧菌)生长的抑制作用,其中氯霉素(30μg/片)、磺胶药+TMP(25μg/片)和呋喃妥因(200μg/片)抑菌力最强、抑菌谱最广;红霉素(15iu/片)、土霉素(30μg/片)无明显抑菌作用。对27种中草药水浸液以平板点种法试抑菌作用。结果表明乌梅、石榴皮、五味子的水浸液具有较强的抑菌活性,可用于配制防病药物饵料。5,7—二氯-8-羟基喹啉对多种病原菌有较强的抑菌作用,药物平板点种法最小抑菌浓度为25mg/L,药浴法有效抑菌浓度低于5mg/L。 相似文献
156.
157.
Perran L. M. Cook Bradley D. Eyre Rhys Leeming Edward C. V. Butler 《Estuarine, Coastal and Shelf Science》2004,59(4):675-685
Benthic fluxes of dissolved inorganic nitrogen (NO3− and NH4+), dissolved organic nitrogen (DON), N2 (denitrification), O2 and TCO2 were measured in the tidal reaches of the Bremer River, south east Queensland, Australia. Measurements were made at three sites during summer and winter. Fluxes of NO3− were generally directed into the sediments at rates of up to −225 μmol N m−2 h−1. NH4+ was mostly taken up by the sediments at rates of up to −52 μmol N m−2 h−1, its ultimate fate probably being denitrification. DON fluxes were not significant during winter. During summer, fluxes of DON were observed both into (−105 μmol m−2 h−1) and out of (39 μmol m−2 h−1) the sediments. Average N2 fluxes at all sampling sites were similar during summer (162 μmol N m−2 h−1) and winter (153 μmol N m−2 h−1). Denitrification was fed both by nitrification within the sediment and NO3− from the water column. Sediment respiration rates played an important role in the dynamics of nitrification and denitrification. NO3− fluxes were significantly related to TCO2 fluxes (p<0.01), with a release of NO3− from the sediment only occurring at respiration rates below 1000 μmol C m−2 h−1. Rates of denitrification increased with respiration up to TCO2 fluxes of 1000 μmol C m−2 h−1. At sediment respiration rates above 1000 μmol C m−2 h−1, denitrification rates increased less rapidly with respiration in winter and declined during summer. On a monthly basis denitrification removed about 9% of the total nitrogen and 16% of NO3− entering the Bremer River system from known point sources. This is a similar magnitude to that estimated in other tidal river systems and estuaries receiving similar nitrogen loads. During flood events the amount of NO3− denitrified dropped to about 6% of the total river NO3− load. 相似文献
158.
Ermanno Crisafi Renata Zaccone Lucrezia Genovese Rosabruna La Ferla Teresa Luciana Maugeri 《Marine Ecology》1989,10(4):365-375
Abstract. Seventy six samples of coastal sediments collected in the Straits of Messina were studied in order to evaluate the effects of an oil spill and the consequent "clean-up" operations on heterotrophic acrobic bacteria.
In addition, in vitro tests were carried out to estimate the effects of five dispersants on the growth and oil degrading capacity of marine strains isolated from the same sediments. 相似文献
In addition, in vitro tests were carried out to estimate the effects of five dispersants on the growth and oil degrading capacity of marine strains isolated from the same sediments. 相似文献
159.
In this study,a novel culture medium that simulates shrimp pond conditions was established to screen nitrite-degrading isolates.The medium was supplemented with nitrite as a nitrogen source and shrimp feed as the major carbon source,to achieve the high nitrogen and low carbon nutritional status found in shrimp farming ponds.Screening using this medium identified potent denitrifying Bacillus isolates,among which Bacillus subtilis M7-1 was considered best.M7-1 was able to completely degrade nitrite-N in 24 h without much consumption of dissolved oxygen.Efficient denitrification activity took place in liquid cultures within a set of non-stringent ranges of pH(5.0–9.0),salinity(0–30)and temperature(25–35℃).The denitrifying enzyme gene was amplified,sequenced and further identified as nirS type.In biosecurity assessments,M7-1 had no negative effects on shrimps at a dose of 106 cfu mL−1.M7-1 could therefore be used in aquaculture to reduce and control the nitrogen concentration,and to promote the development of sustainable and healthy culture systems. 相似文献
160.
《水文科学杂志》2013,58(2)
Abstract The Central Valley of Chile is a zone of intensive agricultural activity. Historically, in this zone, large amounts of fertilizers have been applied and low technological level irrigation methods have been used. Contrary to what might be expected, the existing aquifers in the Central Valley do not contain significant nitrate that could be associated with agricultural activity. This situation leads one to infer the existence of favourable conditions for the occurrence of natural attenuating processes such as denitrification. Favourable conditions may be related to the particular soil and climatic conditions, and/or the structure and dynamics of the existing aquifers. 相似文献