基于全基因组重测序技术的浙江近岸海域耐盐金黄色葡萄球菌(Staphylococcus aureus)耐药机制解析<     

张旨轩  王子言  王泽  刘岩  刘松怡  钱鹏宇  叶欢  韩姣姣  周君  苏秀榕 《海洋与湖沼》2022,53(2):394-404

金黄色葡萄球菌(Staphylococcus aureus)是近岸海域海水中的主要病原菌,严重威胁接触者的安全。抗生素处理是治疗金黄色葡萄球菌感染的重要手段,其耐药性的发生受到了高度重视。采用全基因组重测序与KEGG富集分析结合的方法,对红霉素(erythromycin)、氯霉素(chloramphenicol)和万古霉素(vancomycin)处理后的耐盐金黄色葡萄球菌(S.aureus ZS01)和不耐盐金黄色葡萄球菌(S.aureus 502A)进行耐药机制研究。结果表明,S.aureus 502A经抗生素处理后发生突变的程度大于S.aureus ZS01,二者在经过氯霉素处理发生了更大程度的突变。红霉素、氯霉素和万古霉素处理主要影响了金黄色葡萄球菌的致病能力;红霉素和氯霉素可能通过影响金黄色葡萄球菌脂类的代谢引起其耐药性的变化。除此之外,三种抗生素处理均出现了较多TIGR01741家族蛋白和假设蛋白基因的突变,推测与菌株的耐药性和致病性相关。耐盐金黄色葡萄球菌可通过外排系统作用产生红霉素耐药性,不耐盐菌株因细胞壁成分相关基因的突变提高了对万古霉素的耐受性。研究结果可为耐盐和不耐盐金黄色葡萄球菌的耐药机制及抗生素对金黄色葡萄球菌致病性影响的研究提供基础数据。  相似文献   

陕西柞水地区金盆梁金矿区水系沉积物地球化学特征与找矿方向     

张嘉升  潘爱芳  樊会民  崔丽男 《地球科学与环境学报》2014,(4)

对陕西柞水地区金盆梁金矿区1∶50 000水系沉积物样品中微量元素含量,元素分布、分配、共生组合特征以及主要元素地球化学异常特征进行综合研究,进一步确定该区找矿方向。结果表明:Au是金盆梁金矿区内主要的成矿元素,Ag为主要伴生元素,As、Sb为远程指示元素;泥盆系上统桐峪寺组第五岩性段上亚段是金矿富集的有利层位;石炭系下统二峪河组下亚组下段、泥盆系上统第四岩性段上亚段、泥盆系上统桐峪寺组第五岩性段上亚段为金成矿有利岩性段;矿区经历了至少两期热液活动,金矿体的形成受低级区域变质作用的影响,与岩浆热液活动有关;以Au为主成矿元素圈定的4个综合异常区与地层展布方向一致。位于玄檀沟附近的综合异常区处在金矿富集有利层位,次级构造发育,指示元素异常套合较好,浓集中心明显,Au异常强度高且规模大,并在异常内发现金矿体,因此,位于玄檀沟附近的综合异常区具有很好的找矿前景。  相似文献   

Geothermal occurrence analysis of Dongying Formation in Tanggu area of Tianjin     

LIU Jiulong JIN Baozhen LI Yuanyuan WANG Jige YUE Liyan WANG Xi 《世界地质(英文版)》2014,17(3):163-169

Based on introducing geological structure of Tanggu area, the authors analyzed the sedimentary environment of Paleogene Dongying Formation, and further analyzed its geothermal occurrence. The studied area spans two grade IV tectonic units which are Beitang depression and Banqiao depression. The studied area is mainly located in the Tanggu nose-like structure which is the secondary structure of Beitang depression. Its existence affects the sedimentary distribution of the part strata of Paleogene. The depositions of Dongying Ⅲ （ SQEd3 ） and Dongying Ⅱ （ SQEd2 ） are mainly delta front facies and lake sand bars which are propitious to the geothermal fluid enrichment. The favorable enrichment region of geothermal fluid is located in south Hetou- Tanggu. Most of Dongying Ⅰ （ SQEd1 ） changes into swamp plain deposition, only the northeast part of this area is the distributary riverway facies with developed sand layers. There is the favorable enrichment region of geothermal fluid. The hydraulic connection is weak among the geothermal reservoirs of Dongying Formation and its overlying Guantao and Minghuazhen formations. The underlying Shahejie Formation geothermal reservoir pro- vides a steady stream of fluid supply and ground pressure protection for Dongying Formation geothermal reservoir.  相似文献   

长白山区暗棕壤成土作用地球化学特征     

田密  王志华  白荣杰  孙淑梅  王大勇 《世界地质(英文版)》2014,(3)

以代表性暗棕壤垂直剖面土壤地球化学数据为依据,研究了长白山区特定的森林景观条件下成土过程中元素的分异与富集特征。研究结果表明,在岩石风化过程中主要以CaO、 Na2 O、 Sr淋溶为主,有机酸淋溶过程中SiO2、 Al2 O3、 K2 O部分被淋溶,部分游离铁下渗到土体中下部氧化成铁锰胶膜;微量元素在风化过程中被次生黏土矿物吸附,不同程度地富集,在腐殖质积累过程中Cd、 Zn、Pb、 Mo、 Hg、 Co、 Mn、 Cr等亲生物元素进一步富集于表层土壤, Ce、 Ni、 Sb、 Ti、 V、 Y、 Zr在表生环境中地球化学性质稳定,残留富集于土壤表层。母质层继承和保留了基岩元素地球化学分布的基本特征。  相似文献   

山东苍峄铁矿带沟西-西官庄矿区地下水微量元素Fe~(3+)富集赋存规律          下载免费PDF全文

朱昶  甘延景  宋帅良 《山东国土资源》2014,30(6)

2010年以来,对研究区内水文地质背景及不同层位水质化学测试样品数据分析研究,重点阐述了微量元素Fe3+的富集与含水介质岩性、地下水的酸碱度、地层含水性和氧化还原环境的关系,认为地下水中Fe3+富集赋存的最有利的环境是变质岩类地层;碳酸盐岩和碎屑岩中水化学类型HCO3-Ca型水分布最广,碳酸盐岩的地下水中Fe3+平均含量最低;变质岩中硫酸型(包括复合型)的地下水中Fe3+的平均含量最高,对Fe3+的行为呈正相关关系。地下水中Fe3+含量赋存具以副井及其外围地区为中心高浓度分带性。  相似文献   

Combined effects of light intensity and NH^＋4-enrichment on growth,pigmentation, and photosynthetic performance of Ulva prolifera （Chlorophyta）     

徐智广  吴海一  詹冬梅  孙福新  孙建璋  王广策 《中国海洋湖沼学报》2014,32(5):1016-1023

The aim of this study was to investigate the effects of light intensity and enhanced nitrogen supply on the growth and photosynthesis of the green-tide macroalga, Ulva prolifera. Thalli of U. prolifera were grown in natural or NH 4 +-enriched seawater under two different light intensities for 7 days, and then the growth rate, pigmentation, and photosynthetic performance of the thalli were evaluated. The results show that the relative growth rate(RGR) was markedly higher under the high light level than under the low light level. Enrichment with NH 4 + enhanced the RGR under high light intensity, but did not affect RGR under low light intensity. In low light conditions, NH 4 +-enrichment resulted in a marked decrease in the maximal photosynthetic rate( P m) and the maximum carbon fixation rate( V max), but it did not affect the half saturation constant for carbon( K 0.5) or the ratio of V max to K 0.5, which reflects the carbon acquisition efficiency. In high light conditions, P m, K 0.5, and the dark respiration rate( R d) increased under NH 4 + enrichment, but V max and the V max / K 0.5 ratio decreased. Regardless of the light intensity, NH 4 +-enrichment did not affect the apparent photosynthetic efficiency( α), which refl ects the ability of the alga to use light energy at low light levels. Under both low and high light intensities, the chlorophyll a(Chl a), chlorophyll b(Chl b), and carotenoids(Car) contents in thalli were higher in NH 4 +-enriched than in natural seawater, except that there was a decrease in the Chl b content of thalli in NH 4 +-enriched seawater under low light intensity. Therefore, NH 4 + enrichment improved the growth and photosynthetic performance of U. prolifera under high light intensity, but not under low light intensity. We discuss the possible mechanisms underlying these physiological responses.  相似文献   

Are assemblages of the fireworm Hermodice carunculata enhanced in sediments beneath offshore fish cages?     

Rodrigo Rier  Oscar P&#;rez  Myriam Rodr&#;guez  Eva Ramos  &#;scar Monterroso 《海洋学报(英文版)》2014,33(4):108-111

Abundances of the fireworm Hermodice carunculata were counted through a monitoring assessment study of fish cages in Barranco Hondo（NE Tenerife）. Seven campaigns were conducted from November 2007 to June 2010 and temporal variations were found, as well as differences among sampling stations. The polychaete H. carunculata obtained its highest abundance in sediments beneath fish cages throughout the study period. Thus, the assemblages of this omnivorous species were favoured by the presence of fish cages.  相似文献   

Distribution and sources of heavy metals in the sediment of Xiangshan Bay   总被引：2，自引：0，他引：2  

SUN Weiping  YU Jianjun  XU Xiaoqun  ZHANG Weiyan  LIU Ruijuan  PAN Jianming 《海洋学报(英文版)》2014,33(4):101-107

Heavy metals in the surface sediments and sediment core from the Xiangshan Bay, a mariculture base on the coast of the East China Sea, were determined by inductively coupled plasma mass spectrometry(ICP–MS) in order to evaluate their levels and sources. The results showed that the levels of Cu, Pb, Zn, and Cr in the sediments of the bay have been generally influenced by anthropogenic inputs since the founding of the People's Republic of China. In particular, Cu and Zn were polluted to some extent, as evidenced by high enrichment factors. Organic matter, grain size, wastewater discharge, and low energy hydrodynamic environment played dominant roles in the heavy metal enrichment in the sediments. The ratio of terrigenous source to marine biogenic deposit of trace metals in the sediments was calculated, revealing that terrigenous inputs were the main source of Cu, Pb, and Zn, while biological pellets contributed much more to the enrichment of Cr and Cd. Considering the influence of biological sources on the enrichment of Cd and Cr, and the fact that the sediment has been polluted by Cu and Zn, the development of mariculture and discharge of wastewater into the Bay should be restricted.  相似文献   

中国雨季的一种客观定量划分   总被引：5，自引：0，他引：5  

黄琰  张人禾  龚志强  冯爱霞 《气象学报》2014,72(6):1186-1204

从客观分析角度出发,利用有序样本最优分割法对中国610个台站的气候平均（1961—2010年）候降水序列进行有序分割,给出中国不同区域的雨季定量划分。根据中国13个区域候降水量的气候平均值分布特征,并基于有序样本最优分割法的划分结果需同时满足分割段内波动小、段间差异大的要求,确定了各区域的合理分割数,通过制定3种雨季划分方案,对中国区域雨季进行了细致的定量划分。第1种方案将全年降水划分为雨季和旱季,结果表明,雨、旱两季差异明显的地区出现在华南西部沿海和新疆邻近区域;第2种方案将全年降水划分为雨季相对干期、雨季相对湿期和旱季3个降水阶段,这种特征出现的区域为华南大部分地区、江南地区、长江中下游地区、西南地区东部和南部,以及西北地区中东部;第3种方案将全年降水划分为春雨季、主雨季、秋雨季和旱季,出现这种特征的区域为长三角及淮河流域、黄淮和华北地区、东北地区、西北地区中部、内蒙古地区西部、青藏高原中东部及其以东地区。与已有的中国不同区域降水特征研究结果的比较表明,有序样本最优分割法不仅对中国雨季的划分客观有效,且其划分结果合理并具有明确的气象意义。  相似文献   

张家口市坝下山区岩溶水的富集规律研究     

郝文霞 《地下水》2014,(4):35-37

张家口市坝下地区位于河北省西北部,水文地质情况较复杂,区内碳酸盐岩地层分布较广,地下裂隙岩溶水资源较丰富。根据相关水文地质资料,分析总结了可溶岩地层的厚度、岩性及岩性组合、分布面积、地质构造及地形条件等因素对岩溶水富集规律的影响和控制,阐明了区内岩溶水的供水意义。  相似文献