Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

软土海岸地区测量控制点标志的设立     

苏衍坤  李新国  朱绪伟  苏衍国 《海洋测绘》2007,27(3):43-47

分析了软土、湿地、滩涂地区特殊地理环境对测量控制点的影响;主要探讨了此类地区控制点标志的设计、施工。借助于建筑桩基技术和道桥施工方法,首次成功实现了某三角洲湿地滩涂地区统一布网。几年来,结合滩涂湿地测量、海岸防护和滩涂开发工程对部分控制点进行了初步检测,给出了部分检测数据。应用和检测均表明:桩基标石可较好地解决软土、湿地、滩涂地区控制点布设难题,显著提高软土海岸地区测量控制点标志的稳固性,从而延长控制网的可使用期。  相似文献   

A brief catalogue of failures: Framing evaluation and learning in fisheries resource management     

Kre Nolde Nielsen  Petter Holm 《Marine Policy》2007,31(6):669-680

We propose a framework for enabling a systematic evaluation of a fisheries resource management system, which we define as a feed-back mechanism coupled to a fishery. The resource management system includes four basic functions: diagnostics, intervention, goal setting, and decision making. This model allows for the development of an evaluation framework for fisheries resource management by facilitating a typology of failures. We suggest that the potential for systemic and interdisciplinary learning will be significantly enhanced through the process of developing such a framework.  相似文献   

论宁波东南港湾地区水资源的开发利用途径          下载免费PDF全文

杨晓平 《海洋开发与管理》1995,12(2):62-66

本文在１９８９年对宁波市水资源进行调查的基础上，进一步分析了东南港湾地区水资源的特点及利用现状，并由此而提出了解决水源的对策。  相似文献   

近海污损生物的调查方法     

严文侠  董钰  王华接  严岩  严涛  梁冠和 《热带海洋学报》1994,(4)

近海海区污损生物调查有浮标挂板法和海上设施（浮标及其锚碇系统，Ｍａｒｅｘ水文浮标，移动式钻井平台和固定式生产平台）采样法。由于近海海区污损生物调查工作的特殊性和艰难性，笔者提出了利用已有的海上设施，尤其是固定式平台来积累近海污损生物资料，以便进行生态学研究。  相似文献   

Food Preferences of Dominant Salt Marsh Herbivores and Detritivores     

Carol S.  Rietsma  Ivan  Valiela Ana  Sylvester-Serianni 《Marine Ecology》1982,3(2):179-189

Abstract. Food preferences of six dominant salt marsh invertebrates were studied to identify detritivores and to assess differences in their diets. Animals fed on agar suspensions of dead and live foods in petri dishes with four compartments. Only two foods were included in each dish. Relative palatability was assessed by measuring the amount of suspension removed or by counting the number of feeding marks left on the surface of the suspensions.
Feeding marks reflected differences in mouth parts and feeding mechanisms of the six invertebrates. Melampus bidentatus, Orchestia grillus , and Philoscia vittata preferred dead litter over live tissues of marsh graminoids, blue-green algae, and sulfur bacteria. No preference for detritus from different graminoids was shown by M. bidentatus. Orchestia grillus fed preferentially on Spartina patens detritus. Philoscia vittata preferred detritus from S. alterniflora and S. patens. Blue-green algae and sulfur bacteria were preferred over detritus by Littorina saxatalis , but detritus was preferred over live graminoids. Littorina saxatalis fed preferentially on Juncus gerardi detritus. Blue-green algae, sulfur bacteria, and live graminoids were preferred over detritus by L. littorea. Talorchestia longicornis also preferred blue-green algae.
On the basis of their food preferences, Littorina saxatalis, Melampus bidentatus, Orchestia grillus , and Philoscia vittata were classified as detritivores. Feeding on detritus from different plant species could result in a partitioning of this food resource in the detritus-based food chains of the salt marsh ecosystem.  相似文献   

一种基于拍卖理论的移动Agent资源分配模型   总被引：1，自引：0，他引：1  

郭忠文  刘玉海  刘勇  张阿娜 《中国海洋大学学报(自然科学版)》2003,33(3):439-442

描述了 1种以区间值为价格的移动 Agent资源底价密封拍卖模型 ,并赋予资源所有者参与价格制定的权利  相似文献   

杰氏风暴潮预报方法在珠海及粤西海域的应用     

魏晓宇  窦金来  黄根华  周求赞  陈强  王欣睿 《台湾海峡》2007,26(2):197-203

本文通过杰氏风暴潮预报方法对历史上给珠海和粤西海域造成较大影响的台风增水事件进行后报试验,并通过统计分析方法对原有的预报公式进行订正,总结出适合珠海及粤西海域的风暴潮预报公式.利用5a的时间对珠海及粤西海域的台风增水进行试报,结果表明预报准确率分别达95.0%和87.6%,尤其对一些增水较大、影响较严重的台风增水预报相对更加准确,预报准确率均达90.0%以上.这说明订正后的公式对珠海及粤西海域的风暴潮预报能得到较好的预报效果.  相似文献   

On the instability of “folded” equilibria of a flexible nonstretchable thread attached to the satellite in a circular orbit     

Stanislav D. Furta 《Celestial Mechanics and Dynamical Astronomy》1992,53(3):255-266

The author considers a problem of Lyapunov's stability of relative equilibria of a flexible nonstretchable thread attached to the satellite moving in a circular Keplerian orbit in the first approximation. When it is in the position of relative equilibrium, the thread is known to be situated either along the radius vector of the orbit (the radial equilibrium) or along the circular orbit (the tangential equilibrium) and in each case the thread can be in a folded state. The author shows that folded radial equilibria of the thread are always unstable while tangential ones are unstable if the thread is sufficiently short in comparison with the radius of the orbit. The generalized Chetaev functional has been constructed to prove the instability.  相似文献   

煤矿区地质环境与地质灾害遥感集成监测系统的关键技术     

杜培军  ;陈云浩  ;曹金亮  ;王润福 《测绘软科学研究》2008,(5):14-20

面向“数字矿山”应用系统的需要,在分析煤矿区地质环境和地质灾害特点及其在遥感监测理论依据的基础上,提出了煤矿区地质环境与地质灾害遥感集成监测系统的技术思路和体系结构,强调各种监测技术手段、不同时空尺度、不同数据源和不同主题信息的集成,并针对这一集成系统实现与应用中的多目标遥感信息处理、环境传感器网络应用、遥感监测数据与专题分析模型的集成、数据质量控制等关键问题进行了探讨。  相似文献