Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome     

闫秀英  孙修勤 《中国海洋湖沼学报》2009,27(2)

An open reading frame (lcn61) of iymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector.Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.  相似文献   

Identification of AOSC-binding proteins in neurons     

刘明  聂琴  辛现良  耿美玉 《中国海洋湖沼学报》2008,26(4):394-399

Acidic oligosaccharide sugar chain （AOSC）, a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer＇s Disease （AD） drug candidate. The identification of AOSC-binding protein（s） in neurons is very important for understanding its action mechanism. To determine the binding protein（s） of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry （LC-MS/MS） analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 （AP-2） and amyloid precursor protein （APP）. These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities.  相似文献   

病原分离鉴定及致病性研究     

胡娇  张飞  徐晓津  苏永全  覃映雪  马英  张艺  韩坤煌  鄢庆枇 《海洋与湖沼》2014,45(2):409-417

于2013年4月从宁德患内脏白点病大黄鱼(Pseudosciaena crocea)中分离得到两株优势菌NZBD9和NZBD11, 这两株菌在16—19°C条件下回归感染能引起大黄鱼内脏白点病, 而在7—10°C和24—27°C条件下同样的人工感染不能致病, 从而证实这两株菌为大黄鱼内脏白点病的病原菌。经16S rDNA基因的测序和时间飞行质谱微生物鉴定仪分析, NZBD9 和NZBD11同为变形假单胞菌。药敏性实验结果显示NZBD9对庆大霉素、诺氟沙星和四环素等7种药物高度敏感。组织病理学观察结果显示病鱼的肝脏、脾脏、头肾等组织中均出现明显病症, 如变性和坏死。  相似文献   

海水网箱养殖鲈鱼皮肤溃疡病的防治药物   总被引：21，自引：1，他引：21  

金珊  王国良  赵青松  陈惠群 《台湾海峡》2000,19(2):233-236

本文研究了海水养殖鲈鱼皮肤溃疡病病原菌－哈氏弧菌（Ｖｉｂｒｉｏ ｈａｒｕｅｙｉ）对２４种化学疗剂及１５种中草药的敏感性。结果表明：环丙沙星、氯霉素、庆大霉素、头孢盂多、磺胺＋ＴＭＰ、磺胺甲基异恶唑等６种化学疗剂及地榆、石榴皮、五味子等３种中草药抑菌作用较强,在此基础上进行了药物联用效果试验。  相似文献   

养殖海参主要疾病及防治技术   总被引：39，自引：3，他引：39  

王印庚  荣小军  张春云  孙素凤 《海洋科学》2005,29(3):1-7

首次对中国养殖刺参(Apostichopus japonicus)疾病进行了全面、系统的流行病学调查。结果表明,育苗期的主要疾病有烂边病、烂胃病和化板症,而越冬保苗期和养成期则以腐皮综合症的危害最为严重。详细描述了以上主要病症的流行病学特征,总结出当前海参疾病的病原以细菌为主,兼有霉菌、寄生虫和敌害生物。此外,针对主要病症提出了相应的防治技术和综合预防措施,以指导生产实践。  相似文献   

西沙群岛永兴岛礁坡石珊瑚覆盖率的空间变化   总被引：5，自引：0，他引：5  

施祺  严宏强  张会领  赵美霞 《热带海洋学报》2011,30(2)

连续影像样方调查方法较常用的截线样条法具有空间分辨率高的特点.采用该方法于2008年7月在西沙群岛永兴岛礁坡开展了5条垂直岸线断面和2条平行岸线断面的石珊瑚覆盖率调查.调查发现,永兴岛珊瑚覆盖率存在显著的空间差异,北、西北和东部断面珊瑚分布相对较多,覆盖率较高;西和西南部断面珊瑚分布稀少,覆盖率低.其中北、西北和东部断面沿礁坡方向珊瑚分布明显减少,礁坡中上部30-50m以上、水深5-6m以浅的区域,珊瑚覆盖率高;而礁坡中下部50m以下、水深约6m以深区域,珊瑚分布少,覆盖率低.此外,北和西北部断面还发现由棘冠海星Acanthaster planci和黑皮海绵Terpios hoshinota分别造成的珊瑚白化和珊瑚黑死现象,一定程度上降低了珊瑚覆盖率.永兴岛珊瑚覆盖率的下降以及空间变化格局的形成主要与30年以来日益增加的人类活动和珊瑚礁区过度开发有关.  相似文献   

褐点石斑鱼脱鳞病病原菌的分离与鉴定   总被引：3，自引：0，他引：3  

徐先栋  谢珍玉  欧阳吉隆  王世锋  周永灿 《海洋科学》2012,36(2):67-74

从海南陵水新村港网箱养殖脱鳞病濒死褐点石斑鱼(Epinephelus fuscoguttatus)体内分离到3 株优势菌株XC08061、XC08062 和XC08063, 经回归感染试验确定为致病菌。这3 株细菌经生化鉴定以及16S rDNA 测序分析, 确定均为哈氏弧菌(Vibrio harveyi)。其中, XC08061 对体长为12～14 cm 褐点石斑鱼的半致死剂量为5.8×10² CFU/g 鱼体质量。药敏试验表明, 该菌具有较强的耐药性, 在所检测的25种抗菌药物中, 仅对新生霉素、头孢噻肟、先锋必/舒巴坦、诺氟沙星、依诺沙星和庆大霉素6 种抗菌药物敏感。  相似文献   

大骨节病低氟说的诠释   总被引：1，自引：0，他引：1  

李端生 《吉林地质》2007,26(4):100-104

提出环境低氟是大骨节病原始病因,探索氟元素如何滋养和损伤骨骼系统,寻求有效防治措施。指出地质环境和饮水低氟促成大骨节病发生;在无其他氟源情况下,饮水氟质量浓度小于0.2 m g/L患轻度大骨节病,小于0.1 m g/L患重度大骨节病。人体发生以低氟为中心代谢紊乱,骨基质和骨盐合成不足,阻碍骨生长发育;提出防治大骨节病向人体补氟的具体方法。(6000)  相似文献   

面向高质量发展的中国城市体检方法体系探讨   总被引：9，自引：7，他引：2  

张文忠  何炬  谌丽 《地理科学》2021,41(1):1-12

改革开放40a来,中国的城市规划、建设和管理水平发生了质的改变,但快速城镇化所引发的城市问题日益突出.城市体检概念应运而生,并逐渐从国家战略落实到城市高质量发展的具体实践中.在梳理和解析城市体检背景及其作用的基础上,详细阐述城市体检指标设计的逻辑框架,梳理城市体检各指标间的关系,辨析不同尺度间城市体检的异同,提出中国城...  相似文献   

Fluorine and chlorine behaviour during progressive dehydration melting: Consequences for granite geochemistry and metallogeny          下载免费PDF全文

E.G. Finch  A.G. Tomkins 《Journal of Metamorphic Geology》2017,35(7):739-757

Dehydration melting of biotite is the main control on crustal differentiation in the mid to lower continental crust because this reaction produces the most voluminous and most mobile granitic melts. Biotite breaks down over a broad temperature interval, so the partitioning behaviour of elements between biotite and melt is likely to vary. It has been hypothesized that fluorine may stabilize biotite to higher melting temperatures because biotite is typically F‐rich in ultra‐high temperature (UHT) metamorphic rocks. If true, F would be an important influence on crustal differentiation because not only would it broaden the temperature range of melting but also elevated F concentration decreases melt viscosity. Furthermore, ligand partitioning between biotite and melt may be an important influence on the metallogeny of magmas. This study used electron microprobe analysis of biotite in rocks from the Ballachulish and Rogaland metamorphic aureoles to investigate the concentration of F and Cl in biotite heated to 600–1,000°C. Results show a broad increase in biotite F content (up to 5.04% F) with temperature until 850–920°C, beyond which F content decreases (<2.5% F). Chlorine concentrations in biotite are consistently lower (<1% Cl), and show a progressive decrease after the onset of partial melting. It was found that Mg content, and the processes that control Mg distribution, are most strongly correlated with F and Cl concentration in biotite. Calculations based on these results indicate that F‐enriched biotite could be a significant source of F for continental crust‐derived melts. Generation of a hot, F‐rich melt at UHT conditions could be important for transporting lower crustal metals to the upper crust.  相似文献