Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

西沙礁序列的磁性地层学研究   总被引：3，自引：0，他引：3  

张明书  周墨清 《海洋地质与第四纪地质》1996,16(3):61-65

利用西沙群岛西琛一井３３０ｍ以上井段的１４９块岩心标本，用美国２－Ｇ型超导磁力仪进行了古地磁测定，获得了一个从更新世至中新世晚期包括布容，松山，高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱，为礁序列的划分对比，提供了新的依据。  相似文献   

Comparing the modelled and measured target-strength variability of walleye pollock, Theragra chalcogramma     

Hazen  Elliott L.; Horne  John K. 《ICES Journal of Marine Science》2004,61(3):363-377

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具有国际先进水平的CTD定标检测设备   总被引：1，自引：0，他引：1  

于厚隆  贾肇基 《海洋技术学报》2001,20(1):124-129

在国家863计划的支持下，海洋技术研究成功研制了一套CTD仪器的校准设备，本文介绍了设备的组成，性能，设计经过与SBE设备进行了比较，表明该设备达到了EOCECTD设备的校准要求，同时，该设备是海洋技术研究所开发自有的高度CTD剖面仪的有力工具。  相似文献   

南麂海域台风影响过程中的波浪特征     

黄树生 《海洋湖沼通报》1996,(1):1-8

根据１９８３－１９８９年南麂海洋站在台风影响过程中的实测风和浪资料，分析了该海域的波浪特征。结果表明，这个海域的台风波浪通常是混合浪，在台风影响过程中出现的最大值波高，既有较大波陡的风浪，也有波陡较小的清浪；各向波高的均值变化不大，各向最大波高却有较大幅度的差距；本区的台风浪以４级波高占优，风浪以ＮＮＥ向、涌浪以Ｅ向为常浪向；波高为４级的风浪和涌浪，其周期分别在４．０－４．９Ｓ和７．０－７．９Ｓ之  相似文献   

油田高含水期基于微观渗流机理的宏观岩心水驱油数值模拟研究     

谷建伟  翟世奎  李怀明  薛发玉 《中国海洋大学学报(自然科学版)》2006,36(4):540-544

建立地层孔隙网络模型描述微观孔喉特征,利用逾渗方法计算基于微观渗流机理的相对渗透率曲线,并将之应用到数值模拟计算中,给出考虑微观渗流特征的方程,并进行数值求解,得到地层中流体分布特征。实例分析表明该方法可以得到基于微观特征的剩余油分布数量和形式,为调剖堵水、化学驱提供技术参数,实现微观渗流机理与宏观计算的结合。  相似文献   

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences     

WANG Jun  ZHANG Wen  SU Yongquan  DING Shaoxiong 《海洋学报(英文版)》2004,23(4):709-716

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.  相似文献   

Estimation of birthdates and catch-at-age using length frequency analysis (LFA), with application for skipjack tuna (Katsuwonus pelamis) caught in the Southwest Atlantic     

Andrade  Humber A.; Kinas  Paul G. 《ICES Journal of Marine Science》2004,61(5):798-811

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两个同源地不同路径热带气旋的大尺度场对比分析     

舒锋敏  林良勋 《海洋预报》2007,24(4):102-108

本文利用NCEP/NCAR再分析格点资料,着重对8903号"BRENDA"和0601号"珍珠"两个同源地进入南海后不同路径的典型热带气旋的大尺度环境场进行对比分析。结果表明:西风槽的强弱和东移的快慢、西太平洋副高的形状变化和东退西进、冷空气的强弱和南下路径及热带气旋内部的不对称结构是造成二者移动路经差异的主要原因。另,印缅槽的变化也对其路径差异有一定的作用。  相似文献   

Main Controlling Factors on Hydrocarbon Accumulation and Distribution in Marine Sedimentary Sequences in South China     

蔡立国  周雁  白振瑞 《中国地质大学学报(英文版)》2008,19(6):645-655

Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays.  相似文献