Identification of AOSC-binding proteins in neurons     

刘明  聂琴  辛现良  耿美玉 《中国海洋湖沼学报》2008,26(4):394-399

Acidic oligosaccharide sugar chain （AOSC）, a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer＇s Disease （AD） drug candidate. The identification of AOSC-binding protein（s） in neurons is very important for understanding its action mechanism. To determine the binding protein（s） of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry （LC-MS/MS） analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 （AP-2） and amyloid precursor protein （APP）. These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities.  相似文献   

一种基于多项式外推的局部透射边界位移解(外行波为非平面波情形)   总被引：6，自引：3，他引：3  

张晓志  谢礼立  屈成忠 《地震工程与工程振动》2004,24(1):1-6

一般情况下,通过人工边界向外透射的波动常为非平面波。对于近场非平面波在人工边界上的透射问题,不能采用简单的平面波透射方法。本文针对近场非平面波在人工边界上的透射特点,分析了非平面波沿人工边界法向视波速的变化规律及其近场失稳机制,推导了适合近场非平面波的、人工边界点位移解的不等步距线性外推公式。  相似文献   

Using frontal affinity chromatography to study how silver binds with particulates     

Yurong Cai 《中国地球化学学报》2010,29(3):242-245

Frontal affinity chromatography was applied to characterizing the mechanism of binding of silver with sediment particulates collected from Lake Ontario, Canada. The results showed that there was one major binding site for Ag+ in the particulates. The binding capacity ranges from 6.06 to 1.01 μ·mol·g-1, and the binding constant (lgK) from 6.23 to 7.43 M-1 in 0.005 M ion strength at pH=3-7. The binding capacity and affinity constant were found to be pH-dependent. It is suggested that the particulate surface s...  相似文献   

Similar Adsorption Parameters for Trace Metals with Different Aquatic Particles   总被引：1，自引：0，他引：1  

Beat Müller  Anja Duffek 《Aquatic Geochemistry》2001,7(2):107-126

Conditional surface binding constants and complexation capacities for Zn, Pb, Cd, and Cu were determined from surface titration experiments of heterogeneous natural aquatic particulate matter of different origin and composition. Metals and particles were evaluated in naturally occurring concentration ranges in river water.The adsorption of trace metals can be adequately described with a single conditional binding constant over a wide range of metal : particle ratios. Binding constants for aquatic particles at pH 8.0 are remarkably independent from particle composition and are specific for each metal: log K^ads _Zn = 8.39, log K^ads _Pb = 9.67, log K^ads _Cd = 8.61, log ^ads _Cu = 9.84. From competition experiments with Ca and Pb we extracted a sorption coefficient for Ca of log K^ads _Ca = 2.5 (pH 8.0). Maximum surface binding capacities for all metal ions were found for particles containing high fractions of Mn-oxides which are associated with large specific surface areas. Generally, we found sorption capacities to decrease in the sequence Cu Pb, Zn > Cd.The experiments suggest that the conditional surface binding constants and complexation capacities are applicable to model trace metal adsorption in the concentration ranges of natural waters under conditions similar to the experiments. Results also imply that the chemical nature of particle surface sites is rather uniform in the intermediate concentration range or that the array of binding sites averages out differences in sorption strength over the prevailing concentration range of metal ions, respectively.  相似文献   

黄铁矿及毒砂中负氧化数金的发现及判定     

Oiu Zhaoming  Rang Mei  Qiu Longwei 《吉林大学学报(地球科学版)》1994,(2)

作者在以突破金的氧化态（价态）为先导的研究中，使用光电子能谱对９个金矿区的黄铁矿与毒砂进行了研究。发现它们之中广泛出现有结合能为８３（８２．８～８３．２）ｅＶ的峰，经分析对比后，指认它为Ａｕ峰（４ｆ＿（７／２）。但它的结合能小于Ａｕ￣０（约８４ｅＶ），更小于Ａｕ￣（１＋）及Ａｕ￣（３＋）．根据结合能与氧化态的关系，判定８３ｅＶ峰为负氧化数金（负价金）所引起。即黄铁矿与毒砂中存在有负氧化数金。  相似文献   

基于.NET和MapXtreme的公交查询系统的设计与实现   总被引：1，自引：0，他引：1  

蔡畅  葛文  张小朋 《测绘科学》2008,33(3):163-165

本文详细介绍了基于.NET框架、利用MapXtreme二次开发组件,设计和实现多种情况下的公交换乘查询算法的过程,提出了公交查询系统的解决方案。其中,在数据表的设计上建立了路段与站点的一种一一对应关系的拓扑关系,简化了站点和线路查询的实现过程。在最优换乘方案的设计上采用的是一种基于树形结构的遍历算法,具有较强的实用价值。  相似文献   

煤炭工业的可持续发展与不可持续增长     

田山岗 《中国煤田地质》2003,15(5):1-5,56

增长是数量的增加，是量变；发展，是质量的提升，是质变。囿于煤炭开发过程中的收益递减规律，煤炭资源有效供给不足和对煤烟型污染控制的法律约束导致煤炭需求受限，故而煤炭工业增长具有不可持续之特性。我国煤炭工业的持续增长已经付出了高昂的资源成本、环境成本和安全健康成本。煤炭产量的无序增长，开采技术低水平粗放式的外延扩张，将进一步破坏资源、环境。我国西部将会再现新的楼兰化景象，我们对此深感忧虑。煤炭工业的可持续发展之路为：以煤炭资源的充分合理利用为基础，以环境容量为前提，以废弃物资源化为指导思想，以适度规模的循环经济为目标模式。煤炭工业的可持续发展应以其不可持续增长——有限增长为约束条件。  相似文献   

Seismic performance of square,thin-walled steel tube/bamboo plywood composite hollow columns with binding bars     

《Soil Dynamics and Earthquake Engineering》2016

A thin-walled steel tube/bamboo plywood composite hollow column with binding bars (SBCCB) was developed using transverse binding bars to reinforce a thin-walled steel tube and bamboo plywood composite hollow column. Low reversed cyclic loading tests were performed on 9 SBCCB specimens to observe the damage process and morphology in the SBCCBs. The effects of the slenderness ratio, the net cross-sectional area of the bamboo plywood, the assembly patterns of the cross sections, and the binding bars on the stress and seismic performance were investigated. The results show that the main failure modes of the SBCCBs were cracking of different adhesion interfaces at the base of the column and fracturing of the bamboo plywood. The cross-sectional assembly pattern has a significant effect on the failure modes; increasing the cross-sectional area and the slenderness ratio of the composite column will dramatically improve the seismic performance of the SBCCBs. SBCCBs have excellent elastic deformation and seismic energy consumption characteristics. The binding bars ensure the integrity of the specimen and inhibit the failure of the adhesion interface at the base of the column. Finally, based on the measurement of the axial pressure and the stress-strain curve within elastic range of the composite columns, a simplified mechanical model and a calculation method for bearing capacity was formulated. The calculation values of bearing capacity agree well with the experimental ones, and calculation method can be used to provide guidance for engineering applications of a SBCCB.  相似文献   

嵌入式位图格式与专色印刷技术在“哈同高等级公路沿线详图”中的应用     

孙冠国 《东北测绘》2010,(5):209-210

普通的交通旅游地图册只能满足读者的行车指南要求。那么既能满足读者行车的要求又能满足有关部门维护与勘察要求的图册就不多见了。考虑到有关部门的特殊使用需求与用途,编辑设计了这本采用不规则折页方式的＂沿线详图＂。  相似文献   

关于编制城市旅游地图的几点认识     

傅晶莹 《现代测绘》2012,35(1):56-58

通过实践和思考,本文就有关城市旅游地图的性质、类型及内容要素的分类原则、表现形式等问题作一系统的探讨。  相似文献