Gulf of Mexico hydrocarbon seep communities—IX. Sterol biosynthesis of seep mussels and its implications for host-symbiont association     

Jiasong Fang  Paul A. Comet  Terry L. Wade  James M. Brooks 《Organic Geochemistry》1992,18(6)

The sterol analysis of six hydrocarbon seep mussels (mytilid II and mytilid Ib) from the Alaminos Canyon in the Gulf of Mexico is reported. The sterol composition of the mussel-symbiotic bacteria complex reflects a preponderant synthesis of 4α-methyl sterols (seep mytilid II), and a predominant biosynthesis of 4-desmethyl sterols with some amounts of 4α-methyl sterols (seep mytilid Ib). This suggests a methane-based symbiotic relationship between the mussels and methanotrophic bacteria. It also suggests that the biosynthesis of sterols in the mussel-bacteria complex is completed to the level of cholest-5-en-3ß-ol (mytilid II) or 5α-cholestan-3ß-ol (mytilid Ib).  相似文献   

湘黔地区震旦纪菌藻硅质岩特征及形成机理探讨   总被引：7，自引：0，他引：7  

夏文杰  彭军 《矿物岩石》1993,13(3):21-28

湘黔地区震旦纪菌藻硅质岩化学成分纯净,硅质矿物含量95%以上,几乎都为泥晶—微晶石英,石英结晶度平均为6.35。岩石具菌藻生物结构,以纹层状构造为主。其中丰富的菌藻生物化石,按形态可划分为三种类型。岩石属原生沉积成因,二氧化硅的聚沉一方面通过菌藻有机体的硅化作用,另一方面是菌藻生物化学作用引起微环境变化而导致二氧化硅的聚沉。  相似文献   

Preliminary evidence for human fecal contamination in corals of the Florida Keys,USA   总被引：6，自引：0，他引：6  

Lipp EK  Jarrell JL  Griffin DW  Lukasik J  Jacukiewicz J  Rose JB 《Marine pollution bulletin》2002,44(7):666-670

Corals and reef environments are under increased stress from anthropogenic activities, particularly those in the vicinity of heavily populated areas such as the Florida Keys. The potential adverse impacts of wastewater can affect both the environment and human health; however, because of the high decay rate of bacterial indicators in coral reef waters it has been difficult to document the presence of microbial contaminants and to assign risks in these environments. Here we show initial evidence that microorganisms associated with human feces are concentrated along the surface of coral heads relative to the overlying water column in the Florida Keys. Bacterial indicators (fecal coliform bacteria, enterococci or Clostridium perfringens) were detected in 66.7% of the coral surface microlayer (CSM) samples at levels between five and 1000 CFU/100 ml, but were found infrequently and at low numbers in the overlying water column ( < or = 2.5 CFU/100 ml). Similarly, enterovirus nucleic acid sequences, an indicator of human-specific waste, were detected in 93.3% of the CSM samples and only once in the water column by cell culture. Results show that coral mucus may accumulate enteric microorganisms in reef environments, and may indicate a risk to public and environmental health despite low indicator levels in the surrounding water.  相似文献   

新疆伊犁盆地511砂岩型铀矿床成矿作用机理研究   总被引：5，自引：1，他引：5  

王正其  李子颖  管太阳  张国玉 《矿床地质》2006,25(3):302-311

为探讨511铀矿床的形成机理,通过野外岩心观察和系统样品采集,结合室内化学分析、扫描电镜、电子探针等方法,详细研究了赋存于Ⅴ旋回砂体的铀矿地质特征,对砂岩铀的成矿作用展开了初步讨论。认为Ⅴ旋回层间氧化带可划分为氧化带、氧化_还原过渡带(包括褪色亚带和铀矿石亚带)和还原带;铀矿赋存在过渡带砂岩中,空间展布严格受层间氧化带控制;铀主要以显微浸染状铀矿物(沥青铀矿 铀石)形式散布于炭屑木质细胞腔内壁和砂岩碎屑颗粒的填隙物中,部分以吸附形式赋存于粘土矿物和炭屑;层间氧化带发育是含氧层间承压水与砂岩之间水岩作用的结果。伴随砂岩中有机质、S2-和Fe2 被氧化,形成的有机酸类、SO2-4和Fe3 等组分部分迁移至过渡带沉淀,也是砂岩中铀元素活化迁移、沉淀富集,再活化、再富集的循环过程;层间氧化带及其砂岩铀成矿作用发生于表生环境,始于喜马拉雅早期(66Ma),具有长期性和滚动式向前发展的特点;铀成矿可能与微生物细菌活动及其还原作用密切相关。  相似文献   

Occurrence and Resistance of Pathogenic Bacteria Along the Tietê River Downstream of São Paulo in Brazil     

Wolf-Rainer Abraham  Alexandre José Macedo  Luiz Humberto Gomes  Flavio C. A. Tavares 《洁净——土壤、空气、水》2007,35(4):339-347

The load of pathogenic bacteria, their fate and their dangerousness in the Tietê River were assessed along 100 km starting from the city of São Paulo, Brazil. The data were compared with those from two German rivers. High loads of pathogens were found in the Tietê River near the city of São Paulo (Escherichia coli O157:H7, Shigella flexneri, and Shigella boydii), which were absent 30 km downstream of São Paulo. The antibiotic resistances observed in the Tietê river were rather low and decreased after the major input in São Paulo to significantly lower levels about 30 km downstream. While the Brazilian isolates were more susceptible for ampicillin than the German ones, the reverse was observed for gentamycin. For optimal control of infections in humans critical areas where these bacteria survived longer and their elimination mechanisms should be identified as well as the extent and the origin of antibiotic resistance should be determined.  相似文献   

发光细菌在渔业水域污染物急性毒性快速检测中的应用   总被引：1，自引：0，他引：1  

吴伟  胡庚东  火村英  奚新伟 《广东海洋大学学报》1998,(2)

以明亮发光杆菌为指示生物,对渔业水域中污染物的急性毒性进行了检测,同时研究了PH值、温度对试验结果的影响。研究表明,发光细菌毒性试验是一种快速、简便的毒性测试方法,在温度20～30℃,pH6.0～9.0条件下所进行的测试,其结果与鱼类毒性试验可以互相替代。  相似文献   

南极长城湾及邻近水域表水异养细菌的含量估计及相关因子分析(英)     

CHEN Hao-Wen  ZHU Ming-Yuan 《冰川冻土》1998,(4)

A survey was made of the number of the heterotrophic bacteria in surface seawater of Great Wall Bay and its adjacent waters, Antarctica during the period of austral summers from Dec.1993 to Feb. 1994. The result shows that the number of the heterotrophic bacteria in the surface waterof the surveyed area is about 798 CFU. cm-3, ranging from 53 to 4 250 CFU. cm-3. Comparison of thenumbers for three months shows a declining tendency month by month. Comparing with the previous results, this one shows that the heterotrophic bacteria number changes somewhat, but slightly. It is lower than that in nearshore waters but higher than that in the open area. This shows that there is a positive correlative relationship between CFU and NH4- N, which means that heterotrophic bacteria should be an important agent in NH4-N reproduction in the environment surveyed. There is an significant negative correlation both between CFU and NOZ-N, and between CFU and PO4-P. This means that the NOZ-N, PO.-P, which were decomposed and transformed by bacteria, were used rapidly by phytoplankton.  相似文献   

硅酸盐细菌GY92对伊利石的释钾作用   总被引：26，自引：2，他引：24  

连宾 《矿物学报》1998,18(2):234-238

1992年在贵阳郊区玉米地中分离出一株硅酸盐细菌GY92,为探求GY92对伊利石的释钾作用,对采用的有关材料和方法、相关实验所获的GY92个体形态及培养特征、部分生理生化特征及其对伊利石的释钾作用等结果作了较详细的介绍,同时就GY92对伊利石的释钾机理等问题进行了简单讨论。  相似文献   

Uncertainty in modelling of faecal coliform overland transport associated with manure application in Maryland     

A. K. Guber  Y. A. Pachepsky  A. M. Yakirevich  D. R. Shelton  A. M. Sadeghi  D. C. Goodrich  C. L. Unkrich 《水文研究》2011,25(15):2393-2404

Concerns for microbial safety of surface water facilitate development of predictive models that estimate concentrations and total numbers of pathogen and indicator organisms leaving manure‐fertilized fields in overland flow during runoff events. Spatial variability of bacterial concentrations in applied manure introduces high uncertainty in the model predictions. The objective of this work was to evaluate the uncertainty in model predictions of the manure‐borne bacteria overland transport caused by limited information on the spatial distribution of bacteria in surface‐applied manure. Experiments were carried out at the ARS Beltsville experimental watershed site (OPE3) in Maryland. Dairy bovine manure was applied at a 59·3 t/ha rate on the 3·55 hectare experimental field. Faecal coliform (FC) concentrations in manure measured in 2004, 2005, 2007, and 2009 varied by 4 orders of magnitude each year. Both runoff volume and FC concentrations in runoff water were monitored using a runoff flume equipped with a refrigerated pump sampler. Two runoff events occurred before the manure was incorporated into the soil. A bacteria transport add‐on module simulator of transport with infiltration and runoff (STWIR) was linked with the event‐based kinematic runoff and erosion model (KINEROS2) to simulate convective‐dispersive overland transport, bacteria release from manure, reversible attachment–detachment to soil, and surface straining of infiltrating bacteria. The model was successfully calibrated with the field experiment data. Monte Carlo simulations were carried out to account for the spatial variation in FC in applied manure and uncertainty in the FC distribution in manure caused by the small number of samples. A tenfold and twofold variation in FC concentrations in the runoff were obtained within the 90% probability interval when initial FC spatial distributions in the manure were represented by 5 and 29 samples, respectively. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   

Stormflow dynamics and loads of Escherichia coli in a large mixed land use catchment     

Lucy A. McKergow  Robert J. Davies‐Colley 《水文研究》2010,24(3):276-289

Storm events are major transporters of faecal microbial contaminants, but few studies have reported storm loads or concentration dynamics in relation to discharge or other pollutants, notably fine sediment. Episodically, high loads of faecal contamination during storm flows impact downstream uses of water bodies, particularly contact recreation and shellfish harvesting. We examined the storm dynamics of Escherichia coli, turbidity and discharge in the mixed land use Motueka catchment (2047 km²; 60% forest and 19% pasture) to gain insights into E. coli sources and transport. We also explored different approaches for calculating E. coli loads. Discharge and field turbidity were recorded continuously, and E. coli concentrations were sampled during events, over a 13‐month period near the mouth of the Motueka River. E. coli loads were estimated by interpolation, averaging estimators and by using linear regression with smearing correction of the log‐transformed variables: discharge, turbidity, and both turbidity and discharge. The annual E. coli load was dominated (～98%) by export during events. Comparison of monthly monitoring with the intensive storm monitoring campaign suggests that simple stratification of the sampling into storm and baseflow would greatly improve export estimates. E. coli peak concentrations always preceded discharge and turbidity peaks (which had similar timing). Turbidity can be a useful surrogate for faecal microbes in smaller catchments, but in the Motueka turbidity was no better for predicting E. coli concentration than discharge. Runoff from grazed pasture and direct deposition from livestock are probably the ultimate E. coli sources in the Motueka catchment. However, in‐channel stores seem to dominate E. coli dynamics during events and account for the typical feature of bacterial concentrations peaking ahead of discharge and turbidity. This study demonstrates the importance of storm events to faecal microbial loads and shows that E. coli concentration dynamics may contrast with those of turbidity. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献