热液区管状蠕虫几丁质结合蛋白的克隆表达及功能鉴定     

闫鑫富  阮灵伟 《台湾海峡》2012,31(1):34-38

本文应用PCR技术对四种管状蠕虫几丁质结合蛋白进行了扩增,进而连接到pIZ-FLAG构建了重组表达载体,并在昆虫细胞中实现了几丁质结合蛋白的异源重组表达.我们对几丁质结合蛋白的功能进行了初步研究,亲和活性实验结果表明这四种几丁质结合蛋白对α-chitin具有亲和活性,并且可以辅助几丁质酶,对降解α-chitin和β-chitin均有不同程度的促进作用,且作用效果明显.结果表明,这四种几丁质结合蛋白均为α型,且可以促进几丁质酶的活性,对降解几丁质多糖具有巨大的应用潜力和应用价值.  相似文献   

CCD倾斜仪的差分式结构和电路细分方法研究     

杨江  吴林斌 《大地测量与地球动力学》2012,32(Z1):146-148

介绍通过差分式结构设计和脉冲延迟细分方法解决CCD水管倾斜仪的抗干扰能力较弱、分辨力不高等不足.  相似文献   

“双低”技术在主动脉CTA中的可行性研究     

付传明  陈少波  吴德红  王忠平  徐霖  陈伦刚  余光菊 《CT理论与应用研究》2015,24(3):437-444

目的:评价“双低”技术在主动脉CT血管造影(CTA)中的图像质量和辐射剂量,探讨其临床应用可行性。方法:前瞻性将90例临床似诊为主动脉疾病患者随机分为常规组和双低组(n=45)。常规组:120kVp、对比剂量1.5mL/kg(浓度350mgI/mL)。双低组:100kVp、对比剂量1.0mL/kg(浓度300mgI/mL)。两组的其他扫描参数相同,开启自动管电流调制(ATCM)和Smart prep技术。读取CTA剂量报告中的CT容积剂量指数(CTDIvol)、剂量长度乘积(DLP);记录每位患者对比剂用量并计算碘含量。由2位副主任医师对图像质量进行主观和客观评价,评价内容包括:靶血管和背景的CT值,图像的信噪比(SNR)及对比噪声比(CNR),主动脉及分支血管显示情况和血管锐利程度。采取两独立样本t检验来比较两组的临床资料、辐射剂量、碘含量、图像质量的主观及客观评价等指标。结果:两组在年龄、身高、身体质量指数(BMI)均无统计学差异(P>0.05)。双低组患者接受辐射剂量和碘含量均明显低于常规组,两组差异有明显统计学意义(P<0.05)。两组图像的主观评价差异无统计学意义(P>0.05)。客观评价:两组在主动脉主干及分支的CT值均>300HU,之间差异不具有统计学意义(P>0.05);两组SNR、CNR之间差异不具有统计学意义(P>0.05),双低组图像噪声略高于常规组,噪声在诊断范围内。结论:应用100kVp、对比剂量1.0mL/kg(浓度300mgI/mL),结合ATCM技术在主动脉CTA检查是可行的,优势是有效降低了辐射剂量和对比剂碘量,同时能满足临床诊断要求。   相似文献   

朝阳地震台水管倾斜仪异常核实的认识和讨论     

吴庆明  贾晓东  赵永志  战晓芳  白琳 《防灾减灾学报》2015,(3):105-107

通过朝阳地震台水管倾斜仪异常调查核实,分析其可能的干扰源,并利用滤波和周期分析的数据分析方法进行验证,提高了前兆异常核实的信度和科学含量。  相似文献   

Estimating the settling velocity of bioclastic sediment using common grain‐size analysis techniques          下载免费PDF全文

Michael V. W. Cuttler  Ryan J. Lowe  James L. Falter  Daniel Buscombe 《Sedimentology》2017,64(4):987-1004

Most techniques for estimating settling velocities of natural particles have been developed for siliciclastic sediments. Therefore, to understand how these techniques apply to bioclastic environments, measured settling velocities of bioclastic sedimentary deposits sampled from a nearshore fringing reef in Western Australia were compared with settling velocities calculated using results from several common grain‐size analysis techniques (sieve, laser diffraction and image analysis) and established models. The effects of sediment density and shape were also examined using a range of density values and three different models of settling velocity. Sediment density was found to have a significant effect on calculated settling velocity, causing a range in normalized root‐mean‐square error of up to 28%, depending upon settling velocity model and grain‐size method. Accounting for particle shape reduced errors in predicted settling velocity by 3% to 6% and removed any velocity‐dependent bias, which is particularly important for the fastest settling fractions. When shape was accounted for and measured density was used, normalized root‐mean‐square errors were 4%, 10% and 18% for laser diffraction, sieve and image analysis, respectively. The results of this study show that established models of settling velocity that account for particle shape can be used to estimate settling velocity of irregularly shaped, sand‐sized bioclastic sediments from sieve, laser diffraction, or image analysis‐derived measures of grain size with a limited amount of error. Collectively, these findings will allow for grain‐size data measured with different methods to be accurately converted to settling velocity for comparison. This will facilitate greater understanding of the hydraulic properties of bioclastic sediment which can help to increase our general knowledge of sediment dynamics in these environments.  相似文献   

气象因素对倾斜仪观测干扰特征分析     

狄樑  陆德明  丁建国  刘冬冬  钱文杰 《大地测量与地球动力学》2017,37(8):870-875

采用常熟台水管仪与垂直摆2009～2015年观测数据,研究了气压、降雨、气温、洞温等因素对地倾斜观测的影响特征。结果表明,降雨对地倾斜观测数据干扰最为显著,表现为NS分量发生南倾,EW分量发生西倾,量级上NS分量受干扰程度比EW分量大,垂直摆受干扰程度比水管仪大;气压变化影响表现为短周期扰动,并导致日值曲线出现短时间畸变;气温及洞温变化影响观测值出现明显的年变规律和年变幅变化,对日观测值影响不明显。  相似文献   

利用湖州台形变观测资料检测地震激发的地球球形自由振荡     

方燕勋  卞根发  惠若愚  孙宗强 《中国地震研究》2016,(4):584-593

By using the digital observations of the pendulum tiltmeter, water tube tiltmeter,extensometer and volumetric strainmeter at Huzhou station and with the power spectrum density estimation method,we acquired the_0S_5-_0S_50 fundamental sphere free oscillations caused by the Japan earthquake on March 11,2011,then compared it with the PREM model. The relative errors are mostly bigger than 1.2‰. The extensometer and volume strainmeter can clearly detect _0S_2,_0S_3and_0S_4,which are closely related to the deep structure and earth's interior.  相似文献   

小口径钢管桩与固结灌浆技术联合应用于岩溶地基处理          下载免费PDF全文

曾宪斌  林思波  黄鹏 《探矿工程》2009,36(7):60-62

以工程实践为例,简述复杂岩溶地基处理中小口径钢管桩与固结灌浆技术联合应用的设计、施工工艺及应用效果。  相似文献   

钢管混凝土拱桥海上应用实例     

石彩亭 《中国勘察设计》2009,(9):38-40

近年来钢管混凝土拱桥建设在全国迅速发展,型式多样、各有千秋。本文就大跨度全钢系杆拱在大连新港50万吨级进口原油码头引桥上的成功应用做以下介绍。  相似文献   

φ75 mm双管单动采煤管在薄煤层及粉煤钻探中的应用          下载免费PDF全文

李胜  尹亮先  章术  首照兵 《探矿工程》2012,39(6):50-53

在邻水孔家山井田钻探施工中,由于施工区域煤层厚度不稳定,并且煤层主要以粉煤为主,前期技术准备不充分,造成煤层打丢打薄。通过对其原因的分析及采煤工具的选取和采煤工艺的摸索,引入Ф75 mm双管单动采煤管,并确定了一套简单易行的采煤工艺方法。应用表明,该钻具及工艺能够极大地提高煤样的采取率,防止煤层打薄打丢情况的发生。  相似文献