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91.
以云南省红河州元阳县哈尼梯田核心区域2个典型的旅游村寨(大鱼塘村和普高老寨)为例,对两村寨利益相关者从网络凝聚性、网络互惠性、网络核心边缘、网络传递性、网络经纪人等5个方面展开关系数据分析,结果显示:1)大鱼塘村压力集团内部、压力集团与社区、企业与政府之间的利益关联性和联系的紧密性不足;2)两村当地政府和压力集团与社区之间的互惠性低于整体平均互惠性;3)两村利益网络中边缘结构较核心结构更加明显,这导致了利益关系的不平衡;4)两村当地政府利益集团传递性相对较低,当地社区传递性最高;5)两村中利益集团间缺少部分协调人、守门人、代理人、联络人等角色。最后提出相应建议:1)提升哈尼梯田利益关系由弱利益关系转变为强利益关系,改变利益集团间核心边缘利益关系格局;2)进一步加强各利益集团内部及之间的关联性;3)加强以企业和政府机构为顶点的组间利益传递,让利益流动逐步打破哈尼梯田利益集团之间的核心边缘关系结构;4)重点加强社区在利益关系网络中的参与机会与权力,对社区进行增权;5)利益集团内部要重点培育协调人和顾问角色,利益集团之间要重点培育企业与政府、社区、压力集团之间的守门人、代理人和联络人角色,以及社区与政府和企业之间的守门人、代理人角色。 相似文献
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创伤弧菌是一种可感染人类的河口病原菌。建立快速,特异而敏感的检测方法,有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物(包括两对外部引物和两对内部引物),采用环介导等温扩增技术(LAMP)来检测创伤弧菌。结果显示,本方法的最适扩增温度是63℃,反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出,也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性,其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍,同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较,针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。 相似文献
94.
A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum. 相似文献
95.
Southern California's marine areas are heavily contaminated with dichloro-diphenyl-trichloroethane (DDT) and polychlorinated-biphenyls (PCBs), and fish consumption advisories (FCAs) have been issued throughout the region. Between 2002 and 2003, the Montrose Angler Survey, a large-scale survey of subsistence anglers, was developed and implemented on site in Orange and Los Angeles counties. This survey was intended to assist natural resource trustees in the development of restoration programs that will address injuries to natural resources and restore lost economic services for anglers, but the data were never fully analyzed. The trustees have shown a clear preference for ecological restoration programs that may take years to improve fishing services. In contrast, this analysis, which includes a random-parameter fishing site choice model, demonstrates that simple, inexpensive programs such as better signage to warn of FCAs and transportation to clean sites have the potential to yield substantial benefits quickly. This paper also focuses on how different ethnic minority groups are affected by FCAs, and determines how best to communicate risk information and change fishing behavior through outreach programs. 相似文献
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B. Peng J. M. Sun H. Y. Zhang T. Y. Piao J. Q. Li L. Lei T. Luo D. H. Li Y. J. Zheng R. NAN 《Experimental Astronomy》2004,17(1-3):423-430
Radio frequency interference (RFI) test observations were carried out at one of the candidate Square Kilometre Array (SKA) sites in Guizhou province, following the “RFI Measurement Protocol for Candidate SKA Sites” (hereafter RFI protocol). All data (raw and calibrated) are preserved in some suitable format, such as that set by the international RFI working group of the Site Evaluation and Selection Committee (SESC). An RFI test in December 2003 was performed according to Mode 1 of the RFI Protocol, in order to identify technical difficulties which might arise during a co-ordinated RFI measurement campaign over a period of 1 year. In this paper we describe the current equipment, observational technique and data presentation. The preliminary results demonstrate that the RFI situation at Dawodang depression in Guizhou province makes it quite a promising location for the proposed SKA. Furthermore, the first session of the RFI monitoring program, which was made in May 2004, showed that a complete RFI measurement including both modes 1 and 2 of the RFI Protocol would take about 2 weeks. The possible ways to minimize some limitations of the current equipment are also discussed, which will enable us to meet the RFI protocol. 相似文献
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威海市城市垃圾填埋场选址地质环境适宜性评价 总被引:1,自引:0,他引:1
威海是"最适宜人类居住的地方",但随着城市建设的快速发展,城市垃圾产生量不断增加,垃圾填埋场选址问题亟待解决。以威海市环翠区为研究对象,综合考虑地形地貌、土地利用规划、交通、水利规划、气象、地质、水文地质、工程地质、环境地质等多方面因素及经济效益和环境效益,从地质环境角度出发,选取5个层次共15个评价因子,采用层次—网格法进行了垃圾填埋场选址区划分析研究,划分出垃圾可填埋区、限制填埋区、禁止填埋区。并在限制填埋区和可填埋区,进一步优选出几个垃圾填埋场地的首选场址,可供规划、设计部门参考。 相似文献
100.
PS测井技术是地震勘探方法之一,也是一种简便、快速、准确的原位测试技术。该文介绍了单孔法PS测井的原理及其在铁路工程勘察中的应用,主要包括根据等效剪切波,进行建筑抗震场地类别划分,铁路工程抗震场地类别划分。根据岩土动力学参数,达到评价岩体质量和划分围岩类别目的,利用剪切波速法估算岩土的承载力基本值。通过青岛机场隧道场地的工程实例说明了PS测井技术在岩土工程勘察设计中的应用情况及应用效果。 相似文献