基于18S和ITS-5.8S rDNA基因序列的白色霞水母(Cyanea nozakii)的分子鉴定与检测     

李玉龙  董婧  王彬  孙明  王爱勇  王文波 《海洋与湖沼》2016,47(1):158-165

采用通用引物PCR扩增法,测定了辽东湾海域的白色霞水母(Cyanea nozakii)螅状体、碟状体及水母体的18S以及ITS-5.8S r DNA序列,同时利用Gene Bank数据库中已有同源序列对其进行序列分析及系统分析。结果显示,白色霞水母3个个体的18S和ITS-5.8S r DNA序列完全一致。白色霞水母样品的ITS-5.8S r DNA序列与Gen Bank中未知真核生物的序列高度相似(≥99%),推测该物种可能是早期发育阶段(卵、浮浪幼虫或碟状体)的白色霞水母样品。霞水母属不同种间18S r DNA序列经比对后同源序列长度为1709bp,多态位点数33个;比对后ITS1同源序列长度为368bp,其中变异位点203个,简约信息位点数178个,单变异位点21个。基于18S r DNA基因序列的霞水母属种内和种间平均遗传距离分别为0、0.008,而基于ITS1序列的霞水母属种内和种间平均遗传距离分别为0.019、0.284。基于ITS1的种间遗传距离是种内遗传距离的15倍,适合于进行物种鉴定。NJ系统树的结果也表明同种的不同个体各自聚枝,其聚类结果大致与形态分类吻合。研究表明,ITS基因片段在霞水母不同种间变异较大,更适于大型水母种类鉴定、检测及属内种间水平的系统进化研究。  相似文献   

Apatite Composition of Representative Magnetite‐series and Ilmenite‐series Granitoids in Japan          下载免费PDF全文

Shunso Ishihara  Takeru Moriyama 《Resource Geology》2016,66(1):55-62

Apatites of representative magnetite‐series and ilmenite‐series granitoids were studied in the Japanese Islands. Concentrations of the volatile components F, Cl and SO₃ are differently distributed in apatites of these granitoid series. Apatites are always fluoroapatite. They have weakly higher F content in the ilmenite series than in the magnetite series. In contrast, Cl and SO₃, are significantly concentrated in apatites of the magnetite series compared to the ilmenite series. These characteristics reflect the original concentrations of these components in the host granitic magmas. A high fO₂ seems most important for the S‐concentration as sulfate in apatite of the magnetite series. REE and Y are only erratically high in the studied apatites.  相似文献   

The Legacy of Jedidiah Morse in Early American Geography Education: Forgotten and/or Forgettable Geographer?          下载免费PDF全文

Robert V. Rohli  Merrill L. Johnson 《Geographical review》2016,106(3):465-483

Despite numerous and significant writings by historians of geography and biographers from other disciplines, and his authorship of the first geography textbooks written in and for the new American republic, most geographers are largely unaware of the contributions of Jedidiah Morse in academic geography. Writings about Morse suggest that he had alienated himself from many of his contemporaries early in his career through his authoritarian brand of Calvinistic republicanism, a perceived contradiction of that style with his entrepreneurial ambitions, his role in the controversial Bavarian Illuminati, and a dispute with a noted New England historian. But subsequent, broader intellectual movements sealed Morse's fate as a forgotten geographer (to most), including the end of the Second Great Awakening, Transcendentalism, Darwinism, and the “new,” process‐based geographical thinking inspired by Carl Ritter, Alexander von Humboldt, and Arnold Guyot. Regardless of the reasons for Morse's lost legacy, his contributions to geographical education are important and should be remembered.  相似文献   

LA-ICP-MS测定锡石U-Pb同位素年龄时两种普通铅扣除方法的原理及适用性比较   总被引：1，自引：0，他引：1  

郝爽  李惠民  李国占  耿建珍  周红英  肖志斌  崔玉荣  涂家润 《地质通报》2016,35(4):622-632

根据近年来激光烧蚀电感耦合等离子体质谱仪锡石微区原位U-Pb同位素定年的实验结果,结合相关资料,对实验过程中普通铅扣除的2种主要方法,即谐和图法和等时线法的原理、效果、优点和局限性进行了深入的比较。研究成果表明,2种主要方法各有不同的优点及局限性。在实际工作中,要想获得比较准确的测试结果,需要根据具体矿物样品的年龄范围、总的U和Pb含量、普通铅相对含量、测年精度要求等因素,灵活地选择普通铅扣除的方法。  相似文献   

Defining the Potential of Nanoscale Re‐Os Isotope Systematics Using Atom Probe Microscopy          下载免费PDF全文

Luke Daly  Phil A. Bland  Svetlana Tessalina  David W. Saxey  Steven M. Reddy  Denis Fougerouse  William D.A. Rickard  Lucy V. Forman  Alexandre La Fontaine  Julie M. Cairney  Simon P. Ringer  Bruce F. Schaefer  Daniel Schwander 《Geostandards and Geoanalytical Research》2018,42(3):279-299

Atom probe microscopy (APM) is a relatively new in situ tool for measuring isotope fractions from nanoscale volumes (< 0.01 μm³). We calculate the theoretical detectable difference of an isotope ratio measurement result from APM using counting statistics of a hypothetical data set to be ± 4δ or 0.4% (2s). However, challenges associated with APM measurements (e.g., peak ranging, hydride formation and isobaric interferences), result in larger uncertainties if not properly accounted for. We evaluate these factors for Re‐Os isotope ratio measurements by comparing APM and negative thermal ionisation mass spectrometry (N‐TIMS) measurement results of pure Os, pure Re, and two synthetic Re‐Os‐bearing alloys from Schwander et al. (2015, Meteoritics and Planetary Science, 50, 893) [the original metal alloy (HSE) and alloys produced by heating HSE within silicate liquid (SYN)]. From this, we propose a current best practice for APM Re‐Os isotope ratio measurements. Using this refined approach, mean APM and N‐TIMS ¹⁸⁷Os/¹⁸⁹Os measurement results agree within 0.05% and 2s (pure Os), 0.6–2% and 2s (SYN) and 5–10% (HSE). The good agreement of N‐TIMS and APM ¹⁸⁷Os/¹⁸⁹Os measurements confirms that APM can extract robust isotope ratios. Therefore, this approach permits nanoscale isotope measurements of Os‐bearing alloys using the Re‐Os geochronometer that could not be measured by conventional measurement principles.  相似文献   

Using climate to relate water discharge and area in modern and ancient catchments     

《Sedimentology》2018,65(4):1378-1389

Models relating sediment supply to catchment properties are important in order to use the geological record to deduce landscape evolution and interplay between tectonics and climate. Water discharge (Q _w) is an important factor in the widely used ‘BQART ’ model, which relates sediment load to a set of measurable catchment parameters. Although many of the factors in this equation may be independently estimated with some degree of certainty in ancient systems, water discharge (Q _w) certainly cannot. An analysis of a world database of modern catchments with 1255 entries shows that the commonly applied equation relating catchment area (A ) to water discharge (Q _w = 0·075A^0·8) does not predict water discharge from catchment area well in many cases (R ² = 0·5 and an error spanning about three orders of magnitude). This is because the method does not incorporate the effect of arid and wet climate on river water discharge. The inclusion of climate data into such estimations is an opportunity to refine these estimates, because generalized estimates of palaeoclimate can often be deduced on the basis of sedimentological data such as palaeosol types, mineralogy and palaeohydraulics. This paper investigates how the relationship between catchment area and river discharge varies with four runoff categories (arid, semi‐arid, humid and wet), which are recognizable in the geological record, and modifies the coefficient and exponent of the above‐mentioned equation according to these classes. This modified model yields improved results in relating discharge to catchment area (R ² = 0·95 and error spanning one order of magnitude) when core, outcrop or regional palaeoclimate reconstruction data are available in non‐arid systems. Arid systems have an inherently variable water discharge, and catchment area is less important as a control due to downstream losses. The model here is sufficient for many geological applications and makes it possible to include variations in catchment humidity in mass‐flux estimates in ancient settings.  相似文献   

三峡库区四方碑滑坡稳定性与变形趋势预测   总被引：1，自引：0，他引：1  

肖婷  殷坤龙  杨背背 《中国地质灾害与防治学报》2018,29(1):10-14

三峡水库建成后,库水位周期性涨落和暴雨产生的渗流作用导致大量古滑坡的复活或新滑坡的发生。以库区近水平层状结构的四方碑滑坡为例,依据库水位实际调动,将水位从175 m至145 m不同降速与50年一遇暴雨进行工况组合,计算4种工况下滑坡的稳定性及破坏概率。然后采用Geo-studio软件的Sigma模块对滑坡进行变形模拟,运用R/S分析方法判断滑坡的变形持续性,并结合野外调查情况,综合评价分析四方碑滑坡的稳定性。结果表明:滑坡在各工况下整体均处于基本稳定状态,具有低危险性;变形模拟结果显示滑坡前缘位移最大,与野外调查情况一致;各监测点Hurst指数均介于0.5~1,表明时间序列具有正持续性,在研究的时间限度内滑坡的局部破坏增强,应在汛期加强对滑坡前缘的巡查和预警。  相似文献   

测井地质学前世、今生与未来——写在《测井地质学·第二版》出版之时     

赖锦  王贵文  庞小娇  韩宗晏  李栋  赵仪迪  王松  江程舟  李红斌  黎雨航 《地质论评》2021,67(5):67060005-67060005

测井地质学以地质学和测井学的方法理论为指导,综合运用各种测井信息,来解决基础地质和石油地质的地质问题。经过数十年发展,测井地质学在油气勘探开发各个环节得到广泛应用。非常规油气的兴起使得测井地质学正面临多重挑战和全新探索,亟需建立针对非常规油气的测井地质学综合方法理论体系。本文以《测井地质学·第二版》出版为契机,系统归纳了测井地质学的起源及发展历程、主要研究内容和研究方法流程。然后总结了测井资料与地震、地质信息的匹配性,并分析了不同探测特性测井方法纵向分辨率区间特征。在此基础上评述了测井地质学在井旁构造解析、沉积学特征研究、层序地层划分、地应力方向判别及大小计算、井壁裂缝识别与评价、烃源岩评价以及非常规油气“七性关系”综合评价当中的应用。但由于测井资料的负载能力有限性、测井与地质信息属性不对应性以及测井资料本身的多解性,使得测井地质学在测井—地质转换、非常规油气测井评价及其与人工智能融合方面还存在一定的问题。因此加强基础岩石物理研究,挖掘测井曲线中包含的地质信息,并与人工智能相结合,将拓展测井地质学研究的精度和广度,从而使其未来可更好地应用至非常规油气测井评价等实践工作中。  相似文献   

陕西八卦庙金矿床NE向黄铁矿-石英成矿期成矿物质来源和成矿机制探讨:原位硫同位素证据     

蔡光耀  安芳  袁轶  刘芳  刘炜  张京渤 《地质学报》2021,95(5):1561-1572

八卦庙金矿床位于秦岭造山带凤太铅锌多金属矿田北部,是陕西省规模较大的金矿床之一,已探明金储量约106 t.赋矿围岩是上泥盆统星红铺组浅变质泥质碎屑岩和碳酸盐岩,其成矿过程可划分为3期,分别是①顺层磁黄铁矿-石英成矿期;②NE向黄铁矿-石英成矿期;③裂隙硫化物-方解石成矿期.目前关于NE向黄铁矿-石英成矿期成矿物质来源和成矿机制仍存在争议.本文通过矿相学观察,将NE向黄铁矿-石英成矿期划分为自形磁黄铁矿-黄铁矿-粗粒石英阶段(Ⅰ)、他形黄铁矿-银金矿-细粒石英阶段(Ⅱ)、他形磁黄铁矿-自然金-方解石阶段(Ⅲ)和黑云母阶段(IV),并在此基础上采用激光剥蚀-多接收等离子体质谱(LA-MC-ICP-MS)分析方法对千枚岩围岩中以及不同阶段形成的硫化物进行了原位S同位素测试,结果显示围岩中原生磁黄铁矿的δ34 S值集中于11.6‰～13.0‰之间,介于前人报道的原生黄铁矿δ34 S值变化范围(3.3‰～16.0‰);阶段I形成的黄铁矿的δ34 S值为8.4‰～10.1‰,磁黄铁矿为7.6‰～8.0‰;阶段II黄铁矿的δ34 S值相对较高,为14.0‰～15.9‰;阶段Ⅲ磁黄铁矿的δ34 S值介于6.4‰～8.3‰之间.八卦庙金矿NE向黄铁矿-石英成矿期总体相对富集重硫同位素、离散程度较大,各阶段硫化物的硫同位素值介于矿集区花岗岩和围岩硫之间,显示其具有岩浆硫与地层硫混合的特征.结合阶段I到阶段Ⅲ矿物组合的变化(黄铁矿+磁黄铁矿→黄铁矿→磁黄铁矿),推断阶段IIδ34 S值增高是水岩反应引起的硫化作用所致,而阶段Ⅲ硫同位素值降低可能与岩浆水的加入密切相关.结合成矿物理化学条件,推断该成矿期成矿热液中金主要以Au(HS)2-的形式迁移,阶段I到阶段Ⅱ由水岩反应引起的硫化作用是导致他形黄铁矿-银金矿-细粒石英阶段(Ⅱ)金矿化的主要原因,而岩浆水的混入可能是导致他形磁黄铁矿-自然金-方解石阶段(Ⅲ)金沉淀的主要机理.  相似文献   

柴达木盆地那棱格勒河及其尾闾盐湖锂成矿物源：来自水化学和锶、硫同位素证据   总被引：2，自引：0，他引：2  

李庆宽  王建萍  吴蝉  樊启顺  秦占杰  陈亮  魏海成  都永生  袁秦  李建森  山发寿 《地质学报》2021,95(7):2169-2182

柴达木盆地那棱格勒河尾闾盐湖（一里坪干盐滩、东台吉乃尔盐湖、西台吉乃尔盐湖和察尔汗盐湖别勒滩区段）赋存了我国目前最大的卤水锂矿床。那棱格勒河及其尾闾盐湖锂的物源仍存在一定争议,主要有围岩风化、古湖残留、含盐系地层淋滤、油田水、深部水等,目前缺乏有力的地球化学证据。本文系统采集了那棱格勒河流域及其尾闾盐湖不同水体样品16件,分析了其主、微量元素含量及锶、硫同位素组成。结合前人的研究成果,对区域水体中锂的来源进行了探讨,得出结论如下：那棱格勒河水锂含量（0. 45~0. 79 mg/L）比楚拉克阿拉干河支流（0. 00~0. 05 mg/L）高出一个数量级,其高锂含量主要受洪水河支流的补给;洪水河高锂含量与其上游热泉水的补给有关,该热泉水锂、锶含量高、 87 Sr/ 86 Sr比值偏高, δ 34 S值偏低,与青藏高原典型热泉水地球化学特征（锂含量0. 4~34. 8 mg/L,锶含量0. 07~4. 24 mg/L, 87 Sr/ 86 Sr比值0. 71224~0. 71259, δ 34 S值- 10. 6‰~7. 6‰）较一致。那棱格勒河水（0. 71170）和尾闾盐湖卤水（0. 71143~0. 71156）相似的 87 Sr/ 86 Sr比值,以及研究区河、湖水硫同位素组成符合主要蒸发浓缩过程 δ 34 S值逐渐下降的变化趋势,均证明研究区尾闾盐湖卤水锂资源主要受那棱格勒河的补给;而古湖残留水、盆地西部含盐系地层淋滤水或油田水具有明显不同的水化学特征和锶、硫同位素组成,这些水体对研究区尾闾盐湖锂补给的贡献较小,可忽略不计。  相似文献