大连湾牡蛎的染色体数目   总被引：1，自引：0，他引：1  

董宝贤  党志超  林泰禧 《中国海洋大学学报(自然科学版)》1992,(3)

以大连湾牡蛎的早期胚胎为材料。经染色体数目分析,首次提出大连湾牡蛎的染色体数目为2n=20。为牡蛎遗传育种工作提供依据。  相似文献   

杆状病毒感染越冬亲虾(Penaeus chenesis)的研究——越冬亲虾感染及其垂直传播的可能性   总被引：2，自引：0，他引：2  

包振民  胡景杰  姜明  刘晓云  孔杰  石拓  徐怀恕 《中国海洋大学学报(自然科学版)》1997,(3)

在中国对虾越冬亲虾中检出杆状病毒。经对病虾鳃、前中肠、卵巢组织进行电镜观察，在这些组织中发现大量有囊膜的杆状病毒。病毒粒子大小约为３６７ｎｍ×１２８ｎｍ，核衣壳为３２４ｎｍ×９２ｎｍ。病毒在细胞内不形成包涵体，在核内或在细胞质的发生基质中装配。从病毒的形态特征、感染组织以及典型的发病症状和病理变化，初步认为亲虾感染的病毒与近年广泛报道的造成养殖对虾大规模流行病的主要原因——中国对虾杆状病毒为同一种。卵巢组织也是病毒的主要靶器官之一，亲虾卵巢组织的被感染暗示着该病毒有可能通过生殖细胞而垂直传播  相似文献   

Application of Microbial BOD Sensors in Marine Monitoring     

张悦  王建龙  李花子  施汉昌  竺建荣 《海洋通报(英文版)》2001,3(1)

A strain of yeast, which can endure high osmotic pressure, is employed for the sensitive material of the microbial BOD sensor. Two immobilization methods are used, I.e. Calcium alginate gel be ads and PV A gel beads. The results show that the PVA gel beads is better. The influences of osmosis and heavy metal ions on the yeast entrapped in the PVA gel beads are also studied in the experiment.  相似文献   

Construction techniques for the Taklamakan Desert Highway: research on the construction materials and the results of field tests   总被引：1，自引：0，他引：1  

Changning Jin  Zhibao Dong  Zhinong Li 《Environmental Geology》2006,49(6):915-922

After conducting many laboratory and field experiments, several key technical issues related to the construction of China’s Taklamakan Desert Highway have been satisfactorily resolved. In particular, considerable progress has been made on the dry compaction of a sand sub-base, road design parameters, the creation of a structure that combines a sub-grade and asphalt pavement, analysis of the stability of a sand sub-base strengthened with geotextiles, and on the development of a complete set of construction techniques. The achievements of this research were successfully applied for the first time in the Taklamakan Desert, where the environmental conditions are extremely harsh. The results suggest that the construction of this highway was economical and that the simple construction methods produced a reliable highway. The resulting highway is believed to be the world’s first long-distance graded highway running through a huge desert with migrating dunes.  相似文献   

Sedimentary facies during early stages of flooding in an extensional basin: the Brèche Rouge de Carboneras (Late Miocene, Almería/SE Spain)     

Uwe M. R. Krautworst  Thomas C. Brachert 《International Journal of Earth Sciences》2003,92(4):610-623

The intramontane basins of the Betic Cordilleras (SE Spain) formed subsequent to the main phase of orogenic deformation during the middle Miocene in a close genetic relation to the Trans-Alboran Shear zone. Left lateral movements along a local branch (Carboneras fault zone, CF; strike NE–SW) of this zone played a major role in controlling the formation and dynamics of the Nijar-Carboneras Basin. To the south of the fault, a major phase of strike-slip faulting is recorded during the late Tortonian. The expression of this event is the Brèche Rouge de Carboneras (BRC), which seals a deep denudational surface on top of dislocated fault blocks formed by volcanics of the Cabo de Gata complex and early Tortonian shallow marine calcarenite. The sedimentary facies of this widely distributed unit in the Carboneras-Subbasin mirror the submarine topography and the distribution of the fault zones. Along strike-slip fault zones, autoclastic breccias and neptunian dikes preferentially oriented NW–SE and NE–SW occur, which are interpreted to represent the near-surface expression of the faults. Red limestone forms the groundmass of the autoclastic breccia and infills of neptunian dikes, which exhibit multiple phases of opening of fissures, gravitational sedimentary infill, lithification, and renewed creation of cracks. Steep relief, probably along fault scarps, was mantled by epiclastic volcanic conglomerate with a red carbonate matrix. Well-lithified coarse skeletal limestone rich in planktonic foraminifera formed pavements along sediment starved rocky surfaces in deep water. Laterally, within topographic depressions, the pavement limestone grades into thick accumulations of skeletal rudstone composed of fragmented azooxanthellate corals and stylasterid hydrozoans, which were concentrated by powerful bottom currents and gravitiy flows. Within the shallow water zone of dip slope ramps, cross-bedded calcarenite and calcirudite formed. Based on textures, fabrics and biota, rocks of the BRC were grouped into nine genetic lithofacies which document cryptic, deep-aphotic and shallow-photic environments typical of a sediment starved extensional basin.  相似文献   

星载SAR对雨团催生海面风场的观测研究   总被引：2，自引：1，他引：1  

叶小敏  林明森  袁新哲  丁静  解学通  张毅  徐莹 《海洋学报(英文版)》2016,35(9):80-85

雨团或对流雨是热带与亚热带地区的主要降雨形式,较易被高分辨率星载合成孔径雷达（SAR）探测到。SAR图像上的雨团足印是由大气中雨滴的散射与吸收、下沉气流等共同导致形成的。本文以RADARSAT-2卫星100 m分辨率的SAR图像上雨团引起的海面风场及其结构反演与解译作为实例进行分析。使用CMOD4地球物理模式函数,分别以NCEP再分析数据、欧洲MetOp-A卫星先进散射计（ASCAT）和中国HY-2卫星微波散射计的风向为外部风向,进行了SAR图像的海面风场反演。反演的海面风速相对于NCEP、ASCAT和HY-2的均方根误差（RMSE）分别为1.48 m/s,1.64 m/s和2.14 m/s。SAR图像上一侧明亮另一侧昏暗的圆形信号图斑被解译为雨团携带的下沉气流对海面风场（海面粗糙度）的改变所致。平行于海面背景风场其通过雨团圆形足印中心的剖面上的风速变化可拟合为正弦或余弦曲线,其拟合线性相关系数均不低于0.80。背景风场的风速大小、雨团引起的风速大小以及雨团足印的直径可利用拟合曲线获得,雨团足印的直径大小一般为数千米或数十千米,本文的8例个例解译与分析均验证了该结论。  相似文献   

姬松茸提取物组分体外抗病毒感染活性的研究     

樊廷俊  钱冠兰  杜玉堂  袁文鹏  吕雅萌 《中国海洋大学学报(自然科学版)》2008,38(5)

为了探明姬松茸提取物的体外抗病毒感染活性及其作用方式,进而为水产养殖鱼类高效抗淋巴囊肿病毒(lymphocystis disease virus,LCDV)感染活性物质的开发和鲆蝶类淋巴囊肿病的防治奠定基础,利用热水浸提和酒精沉淀法得到了5种姬松茸提取物组分(E1～5),并利用MTT、细胞病变程度观察等方法研究了5种组分对LCDV感染体外培养大菱鲆鳍细胞(turbot fin cells,TF细胞)的影响作用.细胞毒性实验结果显示,5种组分对体外培养TF细胞均无毒性.细胞病变程度结果表明,本文所得5种姬松茸提取物组分尤其是E5组分具有显著的抗LCDV感染TF细胞的活性.不同方式感染的实验结果进一步显示,5种姬松茸提取物组分抗LCDV感染TF细胞的作用可能主要是通过直接灭活病毒和/或阻断病毒吸附细胞来实现的.  相似文献   

扇贝多肽在体外对免疫细胞活性的影响及其抗紫外线的氧化损伤作用   总被引：9，自引：2，他引：9  

刘晓萍  王玉贞  韩彦弢  于业军  王跃军  王春波 《海洋与湖沼》2001,32(4):414-419

采用噻唑盐（MTT）比色方法研究在紫外线辐射损伤条件下扇贝多肽对免疫细胞的保护作用，并探讨扇贝多肽对胸腺细胞和脾细胞活性的影响，结果表明，扇贝多肽具有抗紫外线氧化损伤的作用，可减轻或抑制紫外线对胸腺细胞和脾细胞的氧化损伤，并且呈剂量依赖性，扇贝多肽在0.5%-10.0%的浓度范围内，其抗氧化能力随浓度的增高而增强，在正常条件下可显著增强免疫细胞的活性，并且可拮抗雌激素对免疫细胞的抑制作用。提示扇贝多肽不仅具有抗氧化损伤作用，而且具有免疫增强使用。  相似文献   

黑鱾血液的生理生化指标研究   总被引：2，自引：0，他引：2  

孙敏  徐善良  唐道军 《台湾海峡》2009,28(4):482-487

本文采用F-820半自动血细胞分析仪和全自动生化分析仪,对黑纪的血液常规指标及血清中的17项生化指标进行了分析测定,并用Wright’s染色法制作血涂片对白细胞进行分类计数．结果表明：（1）黑纪的血红细胞数量为（2．49±0．47）×10^12个／dm^3;白细胞数量为（1．08±0．21）×10^11个／dm^3;在白细胞中以嗜中性粒细胞数量最多,占52．42％±4．67％,其次为淋巴细胞,占25．72％±2．88％;单核细胞体积最大,占总数的3．27％±0．62％．（2）黑纪红细胞脆性大,抗性差,在0．65％～0．70％的NaCl溶液中开始溶血,在0．50％～0．55％的NaCl溶液中完全溶血．（3）黑纪的血红蛋白（HGB）为109．11±15．72g／dm^3,高于一般鱼类,与其较强的耐低氧生理能力相适应．（4）血清中血糖浓度为4．72±1．35mmol／dm^3,明显低于凶猛肉食性鱼类,与其岛礁性生态习性和杂食性相吻合．（5）雌雄鱼的乳酸脱氢酶分别为332．67±188．48U／dm^3和905．66±257．85U／dm^3,雌鱼显著高于雄鱼,可能与繁殖活动相关．  相似文献   

日本七鳃鳗外周血细胞显微结构及类淋巴细胞体外培养   总被引：1，自引：0，他引：1  

逄越  李庆伟 《海洋科学》2012,36(1):23-29

以日本七鳃鳗(Lampetra japonica)为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞;未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15+20%FBS(5%七鳃鳗血清)+0.46%NaCl,在18℃,pH 6.8-7.0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。  相似文献