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Field observations on the variability of crude oil impact on indigenous hydrocarbon-degrading bacteria from sub-Antarctic intertidal sediments 总被引:3,自引:0,他引:3
Oil pollution of the oceans has been a problem ever since man began to use fossil fuels. Biodegradation by naturally occurring populations of micro-organisms is a major mechanism for the removal of petroleum from the environment. To examine the effects of crude oil pollution on intertidal bacteria, we repeated the same contamination experiments on nine different sub-Antarctic intertidal beaches using specifically built enclosures (PVC pipe, 15 cm in inner diameter and 30 cm in height). Despite the pristine environmental conditions, significant numbers of indigenous hydrocarbon-degrading bacteria were observed in all the studied beaches. Introduction of oil into these previously oil-free environments resulted in several orders of magnitude of increase in hydrocarbon-degrading micro-organisms within a few days in some of the studied sites but has no obvious effects on two others. The physical environment of the bacterial assemblage seems to play a major role in the biodegradation capacities. After 3 months of contamination, both remaining oil concentrations and biodegradation indexes differ strongly between the different stations. Thus, chemical and biological parameters reveal a strong heterogeneity of biodegradation capacities between the different sites. 相似文献
957.
I. Corsi S. Bonacci G. Santovito M. Chiantore L. Castagnolo S. Focardi 《Marine environmental research》2004,58(2-5):401
Biochemical characterization of cholinesterase activity (ChE) was carried out on the Antarctic scallop Adamussium colbecki collected in winter 2000 from Campo Icaro (Ross Sea, Antarctica) in order to increase its suitability as a sentinel organism for monitoring the Antarctic environment. The digestive gland, gills and adductor muscle were investigated for substrate specificity and inhibitors sensitivity using acetylthiocholine iodide (ASCh) and butyrylthiocholine iodide (BSCh) as substrates and tetra (monoisopropyl)pyrophosphor-tetramide (Iso-OMPA), 1,5-bis(4-allyldimethylammoniumphenyl)-penthan-3-one dibromide (BW284c51) and the insecticide chlorpyrifos as inhibitors. Effect of in vivo exposure to ZnCl2 was also investigated. All the tissues expressed ChE activity (gill > adductor muscle > digestive gland) and low substrates specificity throughout the hydrolysis of both ASCh and BSCh substrates. Partial (25–29%) and total inhibition (100%) of ChE activity in gills was demonstrated following in vitro incubation with Iso-OMPA and BW284c51 (3 mM), respectively. Concentration-dependent inhibition was also evident with chlorpyrifos in the range 10−4–10−10 M (IC50 10−6) while in vivo exposure to ZnCl2 did not seem to affect ChE activity in the scallop. The potential use of ChE in the A. colbecki as biomarker for monitoring water contamination in the marine Antarctic environment is discussed. 相似文献
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Shigeru Aoki Kazuo Shibuya Akihiro Masuyama Taku Ozawa Koichiro Doi 《Journal of Oceanography》2002,58(3):519-523
Global Positioning System (GPS) measurements of the vertical displacement of fast ice near Syowa Station, Antarctica, were
conducted between April and December 1998 to evaluate measurements of sea level variation derived with a conventional bottom
pressure gauge (BPG). The BPG-derived sea level revealed a seasonal variation of about 0.13 m, with a high in April–June and
a low in November–December. The GPS-derived sea level, combined with observed sea ice thickness, supported the BPG result,
with an RMS error of 0.007 m. Our result also demonstrates that GPS is a powerful technique for monitoring sea level variations.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Observations of the short-term changes of seawater bacterioplankton were carried out during the summer of 1988 in Antarctic coastal surface waters. The studies were designed to compare the variations of bacterioplankton in freemoving and enclosed seawater. Even when a diurnal cycle is present the data collected from the enclosed seawater experiments remain much more constant than the corresponding data in free-moving seawater. The wide range of parameters used in the two experiments reveals common properties which illustrate the major role of displacements of water masses at a fixed sampling station in the temporal changes observed in free-moving Antarctic coastal seawater. 相似文献
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