中国大陆科学钻探（CCSD）微生物研究——地下3900米一株细菌的分离与鉴定   总被引：2，自引：0，他引：2  

王远亮李光玉  东秀珠董海良杨克迁  董志扬 《岩石学报》2005,21(2):540-544

在中国大陆科学钻探科钻一井的3911.28米处利用厌氧培养基分离到一株细菌CCSDFL3900,该菌的最适生长温度为65℃,兼性厌氧,革兰氏染色为阴性,端生芽孢,不运动,电镜观察无鞭毛。利用细菌16SrDNA通用引物对其16SrRNA进行PCR扩增,16SrRNA序列及其进化树分析结果显示,该菌为芽孢杆菌或地杆菌。生化试验检测显示,该菌株能够利用多种糖类发酵产酸,最终鉴定为热坚芽孢杆菌。  相似文献   

A New Family of Sauropod Dinosaur from the Upper Cretaceous of Tianzhen, Shanxi Province, China   总被引：1，自引：0，他引：1  

PANG QiqingDepartment of Resources Environmental Protection  Engineering  Shijiazhuang University of Economics  Shijiazhuang  Hebeiand CHENG Zhengwu Institute of Geology  Chinese Academy of Geological Sciences  Beijing 《《地质学报》英文版》2000,74(2):117-125

A new gigantic sauropod, Huabeisaurus allocates gen. et sp. nov., about 20 m in length and 5 m in height, was discovered in the Upper Cretaceous Huiquanpu Formation, Tianzhen County, Shanxi Province. It is notably different from Diplodocidae, Titanosauridae and Nemegtosauridae in the following aspects: the teeth are strong, peg-like with a length ratio of the tooth crown to tooth root at about 3 to 1; the cervical vertebrae are long with forked spines; the spines in dorsal vertebrae are relatively high, unbifiarcated; the caudal vertebrae are amphicoelous, with anterior neural spines and unbifurcated spines and chevrons; the femur is straight and long, narrow and flat and the tibia and fibula are long and flat. These characters show that the described genus should represent a new family, Huabeisauridae fam. nov. The discovery enriches the sauropod dinosaur record in China, and is quite significant to the study of the taxonomy, evolution, migration, extinction and palaeobiogeographic provincialism of the  相似文献   

细胞生长时期对两种海洋微藻总脂含量和脂肪酸组成的影响   总被引：14，自引：2，他引：12  

魏东  张学成  邹立红  徐年军 《中国海洋大学学报(自然科学版)》2000,30(3):503-509

报道海洋微藻后棘藻 (Ellipsoidion sp.7- 14)和眼点拟微球藻 (N annochloropsis oculata)细胞生长时期对细胞内总脂含量和脂肪酸组成的影响。结果表明 ,两种微藻的总脂均在稳定期含量最高 ,分别占干重的 54.5%和 4 3.3% ,而 EPA、ω- 3多不饱和脂肪酸和总 PUFA的最高比例均出现在对数早期 ,EPA占总脂肪酸的比例可分别高达 2 7.3%和 2 7.7% ,同时总脂的含量却最低 ,分别占干重的 2 2 .9%和 2 2 .0 %。在生长对数期中 EPA是脂肪酸的主要成分 ,而在稳定期中16∶ 0、16∶ 1ω9和 18∶ 1ω9是脂肪酸的主要成分。  相似文献   

First isolation and characterization of a new species of Vibrio from diseased flounder (Paralichthys olivaceus Temminck et Schlegel)     

ZHANG Xiaojun  FANG Hai  CHEN Cuizhen  ZHAN Wenbin  JIN Xiaomin  WANG Xiuyun 《海洋学报(英文版)》2005,24(3):131-140

Appropriate pathogenic bacteria were isolated from diseased (or dead) flounder (Paralichthys olivaceus L) occurring in a mariculture farms of Qinhuangdao, which all diseased fishes expressed bacterial septicaemia. The phenotypic information of the 12 pure cultures was studied extensively, including morphological characteristics, colony characteristics in different media, physiological and biochemical characteristics, and the mole fraction G C ratio of the DNA for representative strain. The results show that the isolates belong to a new species of Vibrio, and are designated as Vibrio qinhuangdaora sp.nov, by the area from which the diseased flounders are collected (Qinhuangdao) based on its biological properties following Rules of International Code of Nomenclature of Bacteria. At the same time,studies on the serum homology ofisolates, and pathogenicity of isolates were conducted by experimental infection. The results show that all the isolates are of serologic similarity, and the isolates have strong pathogenicity to flounder. The studies can extensively reflect the main biological properties of this new species of Vibrio.  相似文献   

一株分离微拟球藻LAMB0002的鉴定     

梁鑫  朱葆华  杨官品  潘克厚 《中国海洋大学学报(自然科学版)》2014,44(7):39-45

微藻的分类鉴定是进一步应用的前提。微拟球藻属的6个种都是单细胞微藻,形态简单,难以用形态学方法进行准确鉴定。本研究分离了一株微拟球藻,结合光学显微镜和透射显微镜观察、脂肪酸和色素组成分析、18SrRNA基因和rbcL基因序列分析,将该藻株鉴定为Nannochloropsis oceanica LAMB0002。本研究进行了微藻分类系统的初步探索,为今后形成系统的微藻分类体系奠定了基础。  相似文献   

一株产琼胶酶海洋细菌的分离与鉴定     

梅建凤  李莎  茅鹤婷  王鸿  应国清 《海洋科学》2014,38(2):71-75

为获得琼胶酶活性高产菌株,对采集的海水样品进行了产酶微生物的分离、筛选和鉴定。经过平板培养预筛和两次摇瓶培养筛选,从浙江省舟山市普陀区近海海水样品中分离到一株琼胶酶产生菌G-5,该菌株液体培养产酶活力可达到413.8 U/mL。G-5菌株的菌落呈乳白色、半透明、表面湿润;菌体革兰氏染色阴性,大小0.58~0.69μm×1.50~2.26μm;光学显微镜下呈短杆状,略有弧形。G-5菌株的16S rDNA序列与NCBI数据库中15个弧菌属(Vibrio)菌株的16S rDNA有98%的同源性,可以确定G-5菌株是一株弧菌(Vibrio sp.)。  相似文献   

南极衣藻（Chlamydomonas sp.ICE-L）谷胱甘肽还原酶基因的原核表达及其条件优化     

刘莹  丁燏  简纪常  吴灶和  缪锦来 《海洋与湖沼》2011,42(6):817-821

采用RT-PCR技术克隆南极衣藻GR基因ORF全长cDNA,然后经酶切、连接等步骤构建其原核表达载体;并对其表达的诱导时间、IPTG浓度、温度进行了优化,以期获得较大量的酶蛋白。结果表明,将构建的原核表达载体pET-GR导入大肠杆菌BL21,可以高效表达融合蛋白;且表达的蛋白均以包涵体的形式存在;经SDS-PAGE电泳...  相似文献   

Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3     

����Ӣ  ����ǰ  �ֽ���  ����  Ф�� 《中国海洋湖沼学报》2011,29(5):917-921

Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.  相似文献   

四种单胞藻和海洋红酵母对刺参浮游幼虫生长与变态成活的影响     

张萍萍  李琪  孔令锋  于瑞海 《中国海洋大学学报(自然科学版)》2011,41(10):30-34

本实验研究4种单胞藻与海洋红酵母(Rhodomonas sp.)搭配以及单胞藻混合投喂对刺参浮游幼虫体长生长及变态存活率的影响。采用三角褐指藻(Phaeodactylum tricornutum)、小新月菱形藻(Nitzschia closteriumf.minutissima)、牟氏角毛藻(Chaetoceros muelleri)和等鞭金藻(Isochrysis galbana)与海洋红酵母分别以1∶0、0.8∶0.2、0.6∶0.4、0.4∶0.6、0.2∶0.8和0∶1的比例搭配投喂刺参浮游幼虫,观测浮游幼虫每天的体长变化以及变态成活率。结果表明,单胞藻与海洋红酵母以适当比例混合投喂能提高刺参幼虫的体长日增长率,单独投喂海洋红酵母的刺参变态成活率也显著高于单独投喂单胞藻组。采用2种单胞藻以0.5∶0.5的比例混合投喂浮游幼虫,结果表明牟氏角毛藻与小新月菱形藻混合投喂组刺参浮游幼虫的变态成活率高于牟氏角毛藻与等鞭金藻的混合组。  相似文献   

晚二叠世种籽化石—新属种的发掘与建立     

陈其奭  朱德寿 《浙江国土资源》1994,(2)

本文根据桐庐县瑶琳镇和平村上二叠统雾林山组（相当于龙潭组）剖面第⑨层中发现的一种奇待的种子化石，经标本描述鉴定，确认其为种子化石的新属种，而建立的奇丽桐庐籽（新属、新种）Tonglucarpusspectabilisgen.etsp.nov,归于晚二叠世。  相似文献