Effects of blue light on gametophyte development of Laminaria japonica (Laminariales, Phaeophyta)     

Shi Cuijuan  Kataoka Hironao  Duan Delin 《中国海洋湖沼学报》2005,23(3):323-329

Laminaria gametophyte was greatly influenced by light in its growth and development. Using light-emitting diodes (LED) as blue and red light sources, we analyzed the light effect on gametophytes development ofLaminaria japonica Aresch. The gametophytes were obtained from zoospores collected in April, May, July, 2003 and September, 2004. We found that the growth of gametophytes was stimulated by increasing intensity of blue light (BL) and red light (RL) illumination, of which BL was obviously stronger than that of RL. The fertilization of gametophytes depended largely on BL, and only sufficient BL illumination could take the reproductive effect. In addition, we noticed that there was a significant difference in light responses for gametophytes developed from zoospore collected in different times. For zoospores released in April, under BL₁ (73.90 μmol photons/m·s), the unicellular female gametophytes and multi-cellular male gametophytes produced eggs and sperms respectively, and further developed towards sporophytes. However, for gametophytes developed in May, July or September, they became multi-cellular and never formed oogonia or antheridia. It is believed that theLaminaria sporangium maturation stage could affect the gametophytes reaction to BL under laboratory culture conditions. Therefore, cryptochrome- or phototropin-like BL photoreceptors is probably involved in BL-induced development ofLaminaria gametophytes. This research was supported by the NSFC (No.40376049) and Japan Society for the Promotion of Science (The Invitation Fellowship for Research in Japan 2002).  相似文献   

海带病烂发生过程中的生理生化变化研究——I．糖、蛋白、总抗氧化能力、SOD的变化     

王丽丽  唐学玺王蒙张培玉  刘传国 《海洋科学进展》2003,21(3):331-335

为了研究海带自然病烂过程中生理、生化变化，在实验生态条件下，人工促使海带病烂，每隔3d取样，对幼苗可溶性糖含量、可溶性蛋白含量、总抗氧化能力、超氧化物歧化酶(superoxide dismutase，SOD)活性的变化进行连续检测。结果表明，在海带幼苗由健康到病烂的过程中，可溶性糖含量表现为逐渐下降的趋势；可溶性蛋白含量先升高后逐渐下降；总抗氧化能力和超氧化物歧化(SOD)的活性呈现出先升高后降低的变化规律。  相似文献   

海带和马尾藻中褐藻胶的糖醛酸组成与序列结构的研究   总被引：1，自引：0，他引：1  

郑乃余  张燕霞  范晓 《海洋与湖沼》1992,23(4):445-453

从中国产海带和3种马尾藻中提取褐藻胶,用IR谱分析其褐藻酸的结构特征,用高分辨~1H-NMR(400MHz)测定无损褐藻酸钠的单体组成和序列结构。IR谱表明了这4种褐藻酸单体组成的差异性。NMR谱的结果表明:海带、海蒿子、海黍子和鼠尾藻中褐藻胶的G组分摩尔分数F_G依次为0.35,0.40,0.45和0.44,而相应的GG二聚重复单元摩尔分数F_(GG)分别为0.21,0.37,0.36和0.34,即海带的F_(GG)明显小于三种马尾藻。文中引入一嵌段分布参数η=F_(MG)/(F_M·F_G),相应的η值依次为0.62,0.13,0.36和0.41,这表明了序列分布的差异性。  相似文献   

褐藻酸降解菌在海带绿烂病发生中的作用   总被引：1，自引：1，他引：1  

王丽丽  唐学玺  王蒙  张培玉 《中国海洋大学学报(自然科学版)》2003,33(2):245-248

从海带幼苗上病烂明显处分离得到 2 3株褐藻酸降解菌 ,并对其菌落形态、细胞形态及革兰氏染色反应等进行了观察。通过对褐藻酸钠的降解实验 ,筛选出 4株具较高降解能力的菌株。感染实验表明 ,分离到的褐藻酸降解菌为海带幼苗病烂的主要病原菌。  相似文献   

Photosynthesis, Respiration and other Factors Influencing the Growth of Laminar/a ochroleuca PYL below 50 Metres in the Straits of Messina     

Edward A.  Drew  John F.  Ireland  Colin  Muir  William A. A.  Robertson John D.  Robinson 《Marine Ecology》1982,3(4):335-355

Abstract. The population of Laminaria ochroleuca in the current swept Straits of Messina was investigated regarding its age structure and photosynthesis. Age structure appears to be determined by sporadic recruitment and the limitation of growth due to peak currents decapitating older plants, which is corroborated by drag force resistance calculations. The calculation of a carbon budget from measurements of photosynthesis and dark respiration both in the laboratory and in situ shows that L. ochroleuca cannot satisfy its carbon demands for growth and respiration at 50 m depth and no satisfactory explanation can be given for its rapid growth between 50 and 100 m.  相似文献   

海带废渣中提取岩藻聚糖硫酸酯的工艺研究     

刘旭  曲桂燕  周裔彬  袁毅  韩丽君 《海洋科学》2012,36(12):25-31

采用响应曲面分析法研究了氯化钙水提法从海带废渣中提取岩藻聚糖硫酸酯的制备技术.在单因素试验的基础上,根据Box-Behnken试验设计原理,通过建立数学模型对提取温度、提取时间、料液比等因素进行显著性和交互性分析研究,获得了最佳提取制备参数.结果表明,提取温度68℃,提取时间6h,料液比1:80时,可以获得3.95％的岩藻聚糖硫酸酯.其中岩藻糖含量为17.47％,硫酸根含量为37.14％.  相似文献   

三十烷醇对海带叶绿体超微结构的效应   总被引：6，自引：0，他引：6  

刘东艳  刘东旭 《海洋湖沼通报》1999,(4):27-30

本文将经不同浓度三十烷醇处理的海带进行电镜切片观察,结果表明经三十烷醇处理后的海带,其叶绿体形态发生显著改变。与对照组相比,叶绿体中的类囊体片层增多,基质浓厚,其外形更为饱满,光合作用的强弱与类囊体片层的多少关系密切。文中从细胞学的水平上分析了三十烷醇促进海带增产的内在原因。  相似文献   

Laminaria gametophyte clone culture and its application in sporeling cultivation   总被引：1，自引：0，他引：1  

Zhou Zhigang  Li Dapeng  Wu Chaoyuan  Liu Haihang 《海洋学报(英文版)》2000,19(2):89-95

IN~UCrIOXIn China, the total annual production of cultivated ham maria japonica reached half milliontons in dry weight these few years. All the sporelings used for artificial cultivation were culturedin greenhouses with controlled temperature. This technique was developed in the mid 1950s(Tseng et al., 1955). The key point of this method is to collect ~spores in summer instead Ofautumn. The garnetophytes and the resulting young sporophytes are cultured at 6 -- 10t generally with sufficient…  相似文献   

不同温度对极北海带幼苗生长及光合特性的影响     

梁洲瑞  刘福利  袁艳敏  杜欣欣  汪文俊  孙修涛  高亚平  王飞久 《海洋科学》2018,42(4):71-78

为研究极北海带幼苗在温度条件下的生理、生态适应性,作者开展极北海带幼苗(1~15 cm)在不同温度下的相对生长速率以及光系统II最大荧光产量(Fv/Fm)、光合作用和呼吸作用等生长和生理响应研究,探讨其最大表观光合速率、饱和光强(Isat)和光补偿点(Ic)等参数。得出以下结论:(1)极北海带幼苗生长的适宜温度范围为9~15℃,温度上限为21℃;(2)极北海带幼苗在21℃胁迫24h以上,可导致其光合系统II产生不可逆损伤;(3)叶片长度对其幼苗的Fv/Fm有显著影响,较大的幼苗相对耐受高温;(4)在5~17℃范围内,极北海带幼苗Ic为4.6~15.4μmol/(m2·s), Isat为52.4~100.3μmol/(m2·s);(5)低温条件下,极北海带幼苗可适应更弱的光照,但相对不耐强光;较大的极北海带幼苗则相对耐强光,其适应更弱光照。本研究结果可为极北海带的人工育苗及养殖提供参考。  相似文献   

植物激素对海带配子体克隆附着的影响   总被引：3，自引：0，他引：3  

韩宝芹  崔竞进  刘涛  王霖 《中国海洋大学学报(自然科学版)》2000,30(4)

实验研究 2 ,4 - D、KT、IAA、NAA四种植物激素对海带配子体克隆附着的影响。结果表明 :2 ,4 - D对附着有较明显的促附着作用 ,最有效浓度为 2 .5mg/L 和 5mg/L,其 4 h的附着率分别是对照的 2 .79倍和 2 .95倍 ;KT的作用较复杂 ,在浓度为 1mg/L 时 ,对附着有明显的促进作用 ,其 4 h的附着率是对照的 2 .3倍 ;IAA对配子体附着没有明显的促进或抑制作用 ;NAA对附着有抑制作用 ,浓度越高 ,抑制作用越强。  相似文献