不同温度对极北海带幼苗生长及光合特性的影响     

梁洲瑞  刘福利  袁艳敏  杜欣欣  汪文俊  孙修涛  高亚平  王飞久 《海洋科学》2018,42(4):71-78

为研究极北海带幼苗在温度条件下的生理、生态适应性,作者开展极北海带幼苗(1~15 cm)在不同温度下的相对生长速率以及光系统II最大荧光产量(Fv/Fm)、光合作用和呼吸作用等生长和生理响应研究,探讨其最大表观光合速率、饱和光强(Isat)和光补偿点(Ic)等参数。得出以下结论:(1)极北海带幼苗生长的适宜温度范围为9~15℃,温度上限为21℃;(2)极北海带幼苗在21℃胁迫24h以上,可导致其光合系统II产生不可逆损伤;(3)叶片长度对其幼苗的Fv/Fm有显著影响,较大的幼苗相对耐受高温;(4)在5~17℃范围内,极北海带幼苗Ic为4.6~15.4μmol/(m2·s), Isat为52.4~100.3μmol/(m2·s);(5)低温条件下,极北海带幼苗可适应更弱的光照,但相对不耐强光;较大的极北海带幼苗则相对耐强光,其适应更弱光照。本研究结果可为极北海带的人工育苗及养殖提供参考。  相似文献   

褐藻酸降解菌的发酵培养及褐藻酸酶对褐藻细胞的解离作用   总被引：7，自引：5，他引：7  

韩宝芹  刘万顺  戴继勋  王海 《海洋科学》1997,21(2):39-43

褐藻酸降解菌埃氏交替单胞菌菌株Ａ１０２发酵培养时褐藻酸酶形成条件研究表明，其产酶的培养基最适褐藻酸钠含量为０．３￣０．６％，氮源为０．５％的蛋白胨，ｐＨ７．５，装量是在５００ｍｌ三角瓶中装培养基２００ｍｌ。  相似文献   

Effects of blue light on gametophyte development of Laminaria japonica （Laminariales, Phaeophyta）     

史翠娟  KATAOKA Hironao  段德麟 《中国海洋湖沼学报》2005,23(3):323-329

1 INTRODUCTION Light acts as an important ambient factor to regulate plant growth. Several critical points in life history for many marine algae are controlled by light. Photobiology of algae has been a discipline of particular interest for a long time (Dring, 1988). Many light effects on algae are photoperi- odic-regulated, such as sporangia formation in red algae Porphyra tenera (Rentschler, 1967) and As- paragopsis armata (Oza, 1976), and erect thalli de- velopment in brown algae Scy…  相似文献   

Immunosuppressive Activities of Fucoidan from Laminaria japonica     

张全斌  李智恩  周革非  牛锡珍  张虹 《中国海洋湖沼学报》2003,21(4):324-328

Effects of fucoidan from Laminariajaponica on 2,4-dinitrochlorobenzene induced de-layed-type hypersensitivity (DTH) reaction and the serum levels of IgG, IgM, complement C3 and C4 were investigated in the present study. Results showed that oral administration of fucoidan atdose of 1.50 and 300 mg/(kg*d) for 9 days before the hapten challenge significantly inhibited 2,4-dinitrochlorobenzene induced delayed-type hypersensitivity reaction; and also inhibited the humoral immunity. Serum C3 and C4 levels were markedly reduced by fucoidan at dose of 1.50 and 300 mg/kg; and serum IgG and IgM levels were reduced by fucoidan at dose of 300 mg/kg. The inhibitory effects of fucoidan on delayed-type hypersensitivity suggested that it may be potential medication for chronic inflammatory diseases in the future.  相似文献   

The extraction of pigments from fresh <Emphasis Type="Italic">Laminaria japonica</Emphasis>     

杨立群  李鹏程  樊守金 《中国海洋湖沼学报》2008,26(2):193-196

The pigments in Laminariajaponica was extracted with six organic solvents and analyzed in spectroscopy analysis. The extractions conditions were screened by an orthogonal test and the quantity of extracted pigments was determined spectroscopically. The results show that： （1） among the six organic solvents, acetone was the most effective one for the extraction; （2） the optimum extraction conditions were as follows： the ratio of S/M （solvent volume/ material weight） was 30 ml/g; fresh seaweed was extracted 2 times in 2 h; （3） the average total content of pigments was 1.85 mg/g （calculated with dry L. japonica）.  相似文献   

褐藻酸降解酶特性的初步研究   总被引：2，自引：0，他引：2  

冯蕾  唐学玺  王艳玲  杨震 《海洋科学》2006,30(2):30-33

对从海带(Laminaria japonica)病烂处分离出的2株别单胞菌属的褐藻酸降解菌(简称菌株A1,A2)进行了褐藻酸钠降解能力及胞外产物中褐藻酸酶特性的初步分析。结果表明,2株褐藻酸降解菌中,菌株A2表现出较强的对褐藻酸钠的降解能力;同时菌株A2所分泌的褐藻酸酶在pH 6.0~8.0相对稳定,30℃以下酶不易失活。在较低离子浓度时,Mn2 ,Ba2 对反应有较强的促进作用,Ag 和Pb2 则有明显的抑制作用。  相似文献   

海带愈伤组织的高效率诱导   总被引：4，自引：0，他引：4  

王希华  秦松  曾呈奎 《海洋与湖沼》1999,30(6):652-657

于１９９４年１月和１１月在青岛太平角和荣成海带育苗场分别采集海带成熟孢子体和幼孢子体,采用培养基诱导方法,以４种培养基诱导海带愈伤组织的效果,成熟海带孢子体的不同部位在ＭＳ固体增富２基中形成愈 组织的能力进行了比较;研究了添另不同成分的ＭＳ固体增富２基对海带愈伤组织的影响,并比较了４种除菌方法的效果。结果表明,ＰＥＳＩ固体２基和ＭＳ固体在是诱导海带愈伤组织的理想培养基,其诱导率分别为７５．５％和６  相似文献   

海带丝状雌配子体孤雌生殖的初步研究   总被引：5，自引：1，他引：4  

白逢伟  秦松  李永祺 《海洋科学》1998,22(6):32-35

比较了两种温度、两种光照强度、4种培养基对海带丝状雌配子体孤雌生殖的影响 ,实验结果表明 ,温度为 10 .0± 0 .5℃、光照强度为 30 0 0 lx、含铁氮磷营养海水培养基是适宜孤雌生殖的条件。在温度、光照强度和培养基 3种因素中 ,培养基组成是影响孤雌生殖速度和效率的最重要因素 ;通过比较找到了 3个孤雌生殖速度较快的丝状雌配子体品系。  相似文献   

海带磷酸烯醇式丙酮酸-羟激酶的研究     

陈敏资  侯和胜  姚南瑜  徐志明  李建之 《海洋与湖沼》1994,25(6):596-600

于1986－1990年，在大连凌水养殖四场采集海带，对磷酸烯醇式丙酮酸羧激酶在海带不同部位、不同发育时期及不同外界条件下活性的变化予以研究。结果表明，该酶是海带体内唯一重要的催化CO2暗固定的酶；此酶活性由海带基部、中部至顶端逐渐减弱；酶活性在用正常水温预处理藻体时最高；正常盐度时酶的活性高于非正常盐度的酶活性；增加NH4+浓度可提高酶的活性。  相似文献   

The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein     

LI Laihao  XUE Changhu  XUE Yong  LI Zhaojie  FU Xueyan 《海洋学报(英文版)》2006,25(4):124-130

1Introduction Thegeneration,reactionandconsequenceof freeradicalsinbiologicalsystemshavereceived muchattentionrecentlyinconnectionwithavariety ofpathologicalevents(BarryandSusanna,1993;FangandLi,1999).LDLoxidizedbyexposureto tracemetalionsCu2 andFe3 (Hein…  相似文献