溶藻弧菌dtd基因的克隆及原核表达条件优化     

陈立明  ;庞欢瑛  ;简纪常  ;吴灶和 《广东海洋大学学报》2014,(3):52-57

根据NCBI数据库中已发表的溶藻弧菌(Vibrio alginolyticus)全基因组序列合成一对特异性引物,应用聚合酶链式反应(PCR技术)扩增溶藻弧菌HY9001菌株dtd基因,将其定向克隆到原核表达载体pET-32a构建重组表达质粒pET-DTD,并对其进行诱导温度、诱导时间、诱导IPTG浓度等条件的优化,最后探究其纯化时最佳咪唑洗脱浓度。结果表明:DTD蛋白成功表达,且其以包涵体的形式存在,在诱导温度37℃、IPTG浓度0.1 mmol/L条件下诱导5 h表达量最高,纯化最佳咪唑洗脱浓度为150 mmol/L。  相似文献   

陕西省每年将安排10亿元作为地质找矿基金     

程伟 《西部资源》2014,(3)

<正>近日,陕西省委、省政府决定,今后每年将拿出近10亿元左右资金,作为地质找矿基金,并主要用于整装勘查区。整装勘查,是在具有一定地质工作程度、成矿条件较好、资源潜力较大的地区,整合资金、技术、人才等生产要素,集中力量开展勘查工作,快速实现找矿重大突破。目前,陕西省主要矿产资源中大多数探明储量在全国排位  相似文献   

在MicrostationJ环境下编制规划设计条件附图的程序设计     

王苏春  张桂莲 《西部资源》2014,(4):174-176

本文介绍了基于Micro Station环境开发的编制规划设计条件附图的量算面积的软件,该软件可以保证量算面积的准确,大大减少工作量,提高内业工作效率。  相似文献   

国家能源局下发通知规范煤制油(气)产业发展     

杨静  王欣婷 《西部资源》2014,(4):57-57

<正>禁建年产20亿立方米及以下规模的煤制气项目和年产100万吨及以下规模的煤制油项目。7月22日,国家能源局下发《关于规范煤制天然气产业科学有序发展的通知》(以下简称《通知》),严格能源转化效率、水耗、排放等产业准入要求,禁止建设年产20亿立方米及以下规模的煤制天然气项目和年产100万吨及以下规模的煤制油项目;并提出,没有列入国家示范的项目,严禁地方擅自违规立项建设。《通知》透露,国家发改委、国  相似文献   

The application and comparison of several flux profile relations in CoLM     

ZhenChao Li  ZhiGang Wei  ZhiYuan Zheng  Hong Wei  Hui Liu 《寒旱区科学》2014,6(1):0073-0080

This paper discusses the important role that flux profile relations play in momentum flux, sensible heat flux, and latent heat flux simulations in CoLM （Common Land Model） and compares the application of three flux profile relation schemes in CoLM by means of the Loess Plateau Land-Atmosphere Interaction Pilot Experiment （LOPEX） of 2005. It reveals that the results simulated by the model barely changed in the original flux profile schemes of the models a~er eliminating the very stable condition and the very unstable condition, and there were only tiny changes in numerical values. This indicates that the corrected terms added to fm（ξm）,fh（ξh） were very tiny and can be ignored under very stable and very unstable circumstances. According to a comparison of the three flux profile relations, the simulation results were basically coherent by using any CoLM： the correlation coefficient of the simulation value and the observed value was 0.89, and this bears on the coherence with the numerical procedures for the flux pro- file relations under unstable circumstances. The simulation results were improved considerably by utilizing the Lobocki flux pro- file schemes, which numerical procedures under unstable circumstances differed significantly fi＇om other three flux profile schemes; in this case the correlation coefficient of the value of simulation and the observed value became 0.95. In the next itera- tion of this study, it will be of great importance for the development of the land surface process model to continue experimenting with the application of some novel flux profile schemes in the land surface process models in typical regions.  相似文献   

基于CNOP方法的台风目标观测中三种敏感区 确定方案的比较研究   总被引：1，自引：0，他引：1  

周菲凡  张贺 《大气科学》2014,38(2):261-272

在目标观测中,敏感区的确定是个关键性的问题。本文详细研究了如何用条件非线性最优扰动（CNOP）方法确定敏感区。提出了三种确定敏感区的方案:水平投影方案、单点能量投影方案以及垂直积分能量方案。比较了三种方案确定的敏感区的差异,分析了它们所阐释的物理意义,讨论了它们的优缺点,并通过理想回报试验考查了不同方案确定的敏感区的有效性。对六个台风个例的应用结果显示,单点能量投影方案与垂直积分能量方案下识别的敏感区较为相似,二者与水平投影方案确定的敏感区则有较大的区别。两种能量方案确定的敏感区更多地反映了环境场对台风的影响,而水平投影方案则反映了台风自身对流不对称性结构对台风发展变化的影响。理想回报试验结果表明,由两种能量方案确定的敏感区对预报误差能量的减小程度以及路径预报的改善程度都要大于水平投影方案确定的敏感区的效果,且垂直积分能量方案确定的敏感区的有效性最高。而在强度预报方面,三种方案对预报效果的改善程度相当。因此,总的说在台风目标观测研究中,利用CNOP方法确定敏感区时,垂直积分能量方案是较佳的方案。  相似文献   

渤海地区平台结构设计环境条件选取分析     

于春洁  王丽勤  吴非  沈晓鹏 《海洋地质译丛》2014,(2):106-110

随着平台的使用年限增长,环境条件随着海洋环境的变化而变得日益恶劣,对于渤海地区,提出了是否采用100年一遇环境条件替换50年一遇环境条件进行结构设计.经过对渤海地区的部分中心平台和井口平台50年/100年环境条件对比分析,提出了对于渤海地区海上平台结构采用100年一遇环境条件替换50年一遇环境条件.  相似文献   

Effect of salinity on growth and first sexual maturity of Exopalaemon carinicauda (Holthuis, 1950)   总被引：1，自引：0，他引：1  

张成松  李富花  相建海 《中国海洋湖沼学报》2014,32(1):65-70

To determine the optimal salinity for growth and first sexual maturity of ExopaIaemon carinicauda, the effects of salinity on growth and reproductive performance of early juvenile prawns of E. carinicauda were evaluated under laboratory conditions. Postlarvae from the same female broodstock were reared at six salinity levels （5, 10, 15, 20, 25, and 30） for 12 weeks. The specific growth rate （SGR） and survival rate （SR） under different salinity levels in the first 6 weeks were calculated and compared. SGR was significantly influenced by salinity. Prawns reared in salinity of 10 grew significantly faster （P〈0.05） than those reared in salinities of 5, 20, 25, and 30. However, ANOVA confirmed that there was no significant effect among the six salinity levels on SR. For the next 6 weeks, the body length （BL）, body weight （BW） at the first sexual maturity, and the age at median sexual maturity （As0） of females were measured and compared. Female prawns reared at salinity of 10 presented significantly shorter A50, but no significant differences among the six salinity levels for BL and BW at the first sexual maturity were observed. Based on the above information, the optimal salinity for growth and first sexual maturity of juvenile E. carinicauda is approximately 10.  相似文献   

Marine bacterium strain screening and pyrethroid insecticide-degrading efficiency analysis     

孙爱丽  刘菁华  史西志  李德祥  陈炯  唐道军 《中国海洋湖沼学报》2014,32(5):1029-1035

A pyrethroid insecticide-degrading bacterium, strain HS-24, was isolated from an offshore seawater environment. The strain, which can degrade cypermethrin(CYP) and deltamethrin(DEL), was identified as Methylophaga sp. The optimal culture and degradation conditions for CYP and DEL by strain HS-24 is pH 7 at 28°C. Under optimum culture conditions, strain HS-24 exhibited a broad degradation concentration range of 100, 200, 400, 600, and 800 mg/L for CYP and DEL. The metabolic intermediates were analyzed by NMR, which provided strong evidence that CYP and DEL removal occurred mainly because of a biological process. The toxicity of the degradation products of strain HS-24 was studied simultaneously by measuring the light output of the luminescence bacterium. This demonstrated that the biodegradation ability of strain HS-24 significantly decreased the toxicity of CYP- and DEL-contaminated aquaculture seawater. Finally, the findings of this paper indicate that strain HS-24 is thus revealed as a biological agent for the remediation of marine aquatic environments.  相似文献   

对新疆鄯善县恰特卡尔塔格铅锌矿地质条件及成矿条件的分析     

赵松峰 《华北国土资源》2014,(2)

通过对区域地层、岩浆岩、构造、区域地球化学特征和矿区地层、岩浆岩、构造、矿区物理化学特征、围岩蚀变的研究论述,证实有该矿的成矿条件,对该区寻找同类型的矿床有较好的指导意义。  相似文献