Effects of Basic Fibroblast Growth Factor and Insulin-like Growth Factor on Cultured Cartilage Cells from Skate Raja porasa   总被引：1，自引：0，他引：1  

樊廷俊  晋凌云  汪小锋 《中国海洋湖沼学报》2003,21(4):305-311

Effects of basic fibroblast growth factor (bFGF) and insulin-like growth factor Ⅱ(IGF-Ⅱ) on cartilage cells from proboscis of skate, Raja porasa Glinther, were investigated in this study. The cartilage cells were cultured in 20% FBS-supplemented MEM medium at 24℃. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF-Ⅱ at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF-Ⅱ was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF-Ⅱ together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF-Ⅱ together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7.  相似文献   

Upper-bound general circulation of coupled ocean–atmosphere: Part 1. Atmosphere     

《Dynamics of Atmospheres and Oceans》2013

We consider the general atmospheric circulation within the deductive framework of our climate theory. The preceding three parts of this theory have reduced the troposphere to the tropical and polar air masses and determined their temperature and the surface latitude of their dividing boundary, which provide the prior thermal constraint for the present dynamical derivation. Drawing upon its similar material conservation as the thermal property, the (columnar) potential vorticity (PV) is assumed homogenized as well in air masses, which moreover has a zero tropical value owing to the hemispheric symmetry. Inverting this PV field produces an upper-bound zonal wind that resembles the prevailing wind, suggesting that the latter may be explained as the maximum macroscopic motion extractable by random eddies – within the confine of the thermal differentiation.With the polar front determined in conjunction with the zonal wind, the approximate leveling of the isobars at the surface and high aloft specifies the tropopause, which is colder and higher in the tropics than in the polar region. The zonal wind drives the meridional circulation via the Ekman dynamics, and the preeminence of the Hadley cell stems from the singular Ekman convergence at the equator that allows it to supply the upward mass flux in the ITCZ demanded by the global energy balance.  相似文献   

基于机载Ka波段云雷达和粒子测量系统同步观测的积层混合云对流泡特征     

张佃国  王烁  郭学良  王洪  樊明月 《大气科学》2020,44(5):1023-1038

利用机载Ka波段云雷达（Airborne Ka-Band Precipitation Cloud Radar, KPR）和粒子测量系统（Droplet Measurement Technologies, DMT）,分析了2018年4月22日黄淮气旋背景系统下积层混合云中对流泡的动力和微物理特征。首先,对Ka波段云雷达观测的山东地区春季36个对流泡样本按照回波强度、水平尺度、回波顶高三个参量进行统计,结果表明平均回波强度为20～30 dBZ的对流泡占69%。对流泡水平尺度为15～30 km,占61%。对流泡最大回波顶高集中在6～8 km,比周边层云高2～4 km。之后,对4月22日积层混合云中的对流泡个例微物理参数进行统计,结果表明对流泡内部以上升气流为主,最大上升气流速度达到1.35 m s?1,平均上升气流速度为0.22 m s?1;对流泡内过冷水含量比较高,最大含水量为0.34 g m?3,平均含水量为0.15 g m?3。对流泡内冰晶数浓度是泡外的5.5倍,平均直径是泡外的1.7倍。结合云粒子图像探头,发现对流泡前沿和尾部冰粒子以柱状和辐枝状为主,而对流泡核心区域冰粒子以聚合体形式存在。冰粒子通过凇附过程和碰并过程增长,过冷水含量不足时冰粒子的凇附增长形成柱状粒子,含量充足时可迅速凇附成霰粒子。对流泡内降水形成的微物理机制不完全相同,主要依赖过冷水含量。当云中有充足的过冷水分布时,高层冰晶通过凇附增长形成霰粒子,通过融化层后形成降水;当云中缺少过冷水时,降水的形成主要通过水汽凝华过程形成冰雪晶,然后雪晶通过聚合过程实现增长。  相似文献   

长石莼（缘管浒苔）（Ulva linza）原生质体再生与分化发育初步研究          下载免费PDF全文

陈群芳  汤文仲  冯子慧  霍元子  何培民 《海洋与湖沼》2011,42(3):397-403

采用细胞酶解技术获得长石莼（缘管浒苔）原生质体,再通过细胞培养观察原生质体再生以及各种分化发育途径。结果表明,长石莼（缘管浒苔）主要存在3种分化发育途径：1）部分原生质体可直接分裂形成有假根或无假根得单细胞苗。2）部分原生质体分裂成规则细胞团或不规则细胞团,最后形成苗簇。3）部分原生质体可发育为孢子囊／配子囊,其中部分...  相似文献   

Establishment of a spleen cell line from large yellow croaker Pseudosciaena crocea and its primitive application in foreign gene transfection     

Ai Sun  Tingjun Fan  Xiuxia Yang  Bin Xu  Xiaohui Xu  Xueyang Guo 《中国海洋大学学报(英文版)》2011,10(4):397-401

A large yellow croaker,Pseudosciaena crocea,spleen(LYCS)cell line was established and the feasibility of using it for foreign gene transfection was evaluaed in this study.Primary culture of LYCS cells was initiated from spleen tissue pieces,which were cultured at 25℃ in Dulbecco’s modiced Eagle medium/F12 medium(DMEM/F12,1:1)(pH7.2),supplemented with 20% fetal bovine serum,carboxymethyl chitosan,chondroitin sulfate,basic fibroblast growth factor(bFGF)and insulin-like growth factor-I(IGF-I).The cultured LYCS cells,in fibroblast shape,proliferated to 100% confluency 20 days later.Chromosome analyses indicated that the LYCS cells exhibited chromosomal aneuploidy with a modal chromosome number of 48 which displayed the normal diploid karyotype of P.crocea(6m+6sm+36t,NF=60).A LYCS cell line,with a population doubling time of 48.7 h at passage 60,has been established and subcultured to passage 70.Transgenic feasibility test demonstrated that positive green fluorescence protein(GFP)expression was observed in LYCS cells after pcDNA3.1-GFP plasmid transfection.In conclusion,a continuous foreign gene trans-fection feasible LYCS cell line has been established successfully.The cell line might serve as a valuable tool for studies of transgenic breeding and has potential applications for different kinds of cytotechnological studies.  相似文献   

湛江地区光裸星虫的生殖细胞发育和生殖周期   总被引：2，自引：0，他引：2  

王庆恒  杜晓东  黄洪艳  秦红贵 《广东海洋大学学报》2005,25(1):5-9

采用活体观察和常规石蜡切片技术对湛江地区光裸星虫生殖周期、生殖细胞的发育及形态结构进行研究。结果表明:星虫雌雄异体,无肉眼可见生殖腺结构,原生殖细胞在体腔液中发育;雌性生殖细胞根据其卵膜的形成和卵黄积累情况可以分为4个发育阶段,小生长期、大生长期、成熟前期和成熟期;雄性生殖细胞也分为增殖期、生长期、成熟期和变态期4个阶段;成熟的生殖细胞经肾管排出体外。生殖季节为5~9月,其中7~8月为高峰期。  相似文献   

基于径向基函数神经网络的混合像元分解   总被引：10，自引：1，他引：10  

张彦  邵美珍 《遥感学报》2002,6(4):285-288

遥感图像中普通存在着混合像元。对这部分像元进行分类（即混合像元分解）是遥感图像处理中的难点。基于主分量分析的混合像元分解 法是一种较为成熟的算法，但它存在着计算量大，适应性差等缺点。在深入研究混合像元分解原理的基础上，提出了用径向基函数神经网络拟合分解结果超平面，以实现混合像元分解的算法，实验结果证明：该算法的结果与基于主分量分析的混合像元分解算法结果相近（相关系数达到0．00），而计算量大大减少，具有较强的适应性。  相似文献   

The Perspective of Deep-sea Science Research and the Establishment of Modern Hydrothermal Laboratories in China     

I-Ming CHOU 《地质学报》2013,87(Z1):3-4

  相似文献   

http://www.sciencedirect.com/science/article/pii/S1674987112001028     

Tatsuyuki Yamamoto  Hideo Akiyoshi  Keisuke Yoshikiyo  Tetsuya Takahashi  Yukiko Tanabe  Sakae Kudoh  Satoshi Imura  Naoyuki Yamamoto 《地学前缘(英文版)》2013,4(6):647-653

A study on the effect of the solar ultra-violet radiation on the human skin fibroblast cells revealed that the production of matrix metalloproteinase-2 was inhibited by the radiation.A CO2 incubator connected by optical fibers to a reflector telescope for collecting the solar light was built at Syowa station by the 49th Japanese Antarctica Research Expedition.The direction of the telescope was continuously controlled by a sun-tracker to follow the movement of the Sun automatically.The intensity of the collected light was monitored by a portable spectrophotometer housed inside.The human skin fibroblast cells were incubated in the CO2 chamber to investigate the effect of the solar radiation at Syowa station and were compared with those reference experiments at a laboratory in Japan.The results showed cell damage by strong UV radiation.The production of matrix metalloproteinase-2 was prompted by the moderate UV-B,but was inhibited by the strong UV-B radiation,as studied under laboratory conditions in Japan.The effect of strong solar radiation at Syowa station involving the radiation of UV-B region was estimated to be of the same extent of the radiation caused by an artificial UV-B light with the intensity more than 50 mJ/cm2.  相似文献   

Biosorption of Procion Red MX 5B by Bacillus subtilis and Its Extracellular Polysaccharide: Effect of Immobilization     

Arthur Raj Binupriya  Muthuswamy Sathishkumar  Chang Sub Ku  Sool Il Yun 《洁净——土壤、空气、水》2010,38(8):775-780

Bacillus subtilis and its extracellular polysaccharide (EPS) were used in free form as well as immobilized form as biosorbent for the removal of an anionic dye Procion Red MX 5B. Low pH was favourable for biosorption. Immobilization resulted in reduced biosorption of the dye. The presence of functional groups responsible for the high adsorption capacity in free cells (FC) and EPS was confirmed by FTIR analysis. High Q_max and b values were noted in the case of FC and free EPS in contrast to immobilized cells and EPS. The kinetics data showed that the adsorption system followed pseudo‐first‐order reaction at low dye concentration. Desorption of the dye was found to be 100% in 1 N NaOH. In the case of immobilized biomass and EPS the alginate was found to be unstable under high alkaline conditions of NaOH.  相似文献