Triploidy induction with heat shocks toPenaeus chinensis and their effects on gonad development     

Li Fu-hua  Zhou Ling-hua  Xiang Jian-hai  Liu Xu-dong  Zhu Jin-zhao 《中国海洋湖沼学报》1999,17(1):57-61

Heat shocks effectively produced triploids inPenaeus chinensis. Fertilized eggs heat shocked (28–32°C) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy induction rates of 39%–75%. Several triploid populations were cultured to 10 cm. In a triploid population, two kinds of ovaries were observed. Histological examination showed apparent differences between these two kinds of ovaries, whereas among male shrimp, there were no such differences. Contribution No. 3465 from the Institute of Oceanology. Chinese Academy of Sciences. The study was supported by the International Foundation for Science, Asian Fisheries Society and Chinese Climbing Project PDB 6–2, and Chinese National 863 Project (No. 863-819-01-05).  相似文献   

鱼塭产斑节对虾(Penaeus monodon)催熟繁殖技术研究     

杨小立  黄爱贞  黄德 《广东海洋大学学报》1999,(2)

以综合催熟措施和移植精荚手术相结合的技术方法,解决了鱼塭产斑节对虾催熟效应期长,产卵率低,易蜕壳失去精荚等难题。使用 ２７８ 尾雌虾,催熟率达９０％ 以上,在４０ ｄ 内共产卵６０８ 尾次,卵量１８ ５３０ 万粒,孵出无节幼体１１ ９９８ 万尾,平均孵化率 ６４．７％ ,平均每尾雌虾孵出无节幼体 ４３．１ 万尾,育成商品虾苗 １ ０２８．５ 万尾,育苗平均成活率 １３．６％ ,试养成活率５７．１％  相似文献   

A STUDY ON PATHOGENS OF CHINESE PRAWN (PENAEUS CHINENSIS) VIRUS DISEASES     

孙修勤  张进兴 《中国海洋湖沼学报》1995,(3)

This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis. O'sbeck)is due to three kinds of viruses: epithelium envelope baculovirus of Penaeus chinensis (EEBV-PC, de-tected by the authors in 1993), infections hypodermal and hematopoietic necrosis virus, andhepatopancreatic parvo-like virus, and that the first two viruses seem to be the main pathogens of theepidemic in the northern regions in 1993.  相似文献   

Studies on immunodiagnosis of hepatopancreatic parvo-like virus diseases ofPenaeus orientalis     

Sun Xiuqin  Wang Wenxin  Shao Jijun  Zhang Jinxin  Lu Ying  Xue Qinggang  Song Qinyun 《中国海洋湖沼学报》1993,11(3):245-248

This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results. This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp. The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms.  相似文献   

STUDIES ON IMMUNODIAGNOSIS OF HEPATOPANCREATICPARVO-LIKE VIRUS DISEASES OF PENAEUS ORIENTALISII. RAPID EARLY DIAGNOSIS     

孙修勤  王文兴  邵济钧  张进兴  卢颖  薛清刚  宋庆云 《中国海洋湖沼学报》1993,(3)

This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results.This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp.The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms.  相似文献   

蜕壳促长素对斑节对虾、刀额新对虾蜕壳和生长的作用     

吴琴瑟  杨睿  刘翠红 《广东海洋大学学报》1996,(2)

在对虾人工配合饲料中添加蜕（脱）壳促长素，喂养斑节对虾（ＰｅｎａｅｕｓｍｏｎｏｄｏｎＦａｂｒｉｃｉｕｓ）和刀额新对虾（ＭｅｔａｐｅｎａｅｕｓｅｎｓｉｓＤｅＨａａｎ），观察其促进蜕壳、生长的作用。试验证明：饲料中添加不同浓度（０．５‰。、１．０‰、１．５‰）的蜕壳促长素，均不同程度地促进对虾的蜕壳和生长。添加浓度为１．５％。效果最为明显。值得在生产中应用。  相似文献   

“海参-对虾”混养海水池塘的食物网结构及营养层级研究     

郭凯  赵文  王珊  刘保占  张鹏 《海洋学报(英文版)》2016,35(4):58-62

The food sources of aquacultured Apostichopus japonicus and the trophic levels of organisms in a sea cucumber(A. japonicus) and prawn(Penaeus japonica) polyculture system in a saltwater pond in Zhuanghe, Liaoning Province were examined using carbon and nitrogen stable isotopes. Across organisms, δ13C ranged from(–25.47±0.20)‰ to(–16.48±0.17)‰(mean±SD), and δ15N ranged from(4.23±0.49)‰ to(12.44±0.09)‰. The δ13C and δ15N contents of A. japonicus, P. japonica and Fenneropenaeus chinensis were comparatively higher than those of other organisms. Values of δ13C and δ15N revealed that P. japonica, Hemigrapsus sanguineus and Neomysis japonica comprised the largest component of the diet of A. japonicus. The mean trophic level of the organisms in this saltwater pond polyculture system was(2.75±0.08). P. japonica, A. japonicus, F. chinensis,Synechogobius hasta and Neomysis japonica were in the 3rd trophic level(2–3); jellyfish, H. sanguineus and zooplankton were in the 2nd trophic level(1–2); and Enteromorpha prolifera, benthic microalgae, periphyton and suspended matter primarily consisting of phytoplankton, bacteria and humus were in the primary trophic level(0–1).  相似文献   

碱性成纤维细胞生长因子与胰岛素样生长因子Ⅱ对中国对虾淋巴细胞培养物的协同诱导作用   总被引：2，自引：0，他引：2  

樊廷俊  汪小峰  史振平  晋凌云  汪岷  朗刚华  童裳亮 《海洋学报》2001,23(5):141-145

对虾的传染性疾病是限制对虾养殖业发展的主要因素.目前至少发现了17种对虾病毒病并鉴定出了30多种对虾病毒[1～5].虽然已在其宿主动物---虾中对对虾病毒病进行了多方面的研究,但为了分离、纯化和鉴定这些病毒,必须建立一个适宜于对虾组织的体外培养系统.繁殖并弄清病毒的分子结构及其理化特性是研究病毒病、检测和治疗病毒病的重要手段,而发展对虾细胞的体外培养和建系技术是解决这些问题的关键之一.  相似文献   

Sea water environment copper requirement of egg hatching and naupliar metamorphosis of Peaneus chinensis     

Yuan Youxian  Qu Keming  Liu Libo  Gao Chengnian  Zhang Duxi 《海洋学报(英文版)》1995,14(3):421-428

ＳｅａｗａｔｅｒｅｎｖｉｒｏｎｍｅｎｔｃｏｐｐｅｒｒｅｑｕｉｒｅｍｅｎｔｏｆｅｇｇｈａｔｃｈｉｎｇａｎｄｎａｕｐｌｉａｒｍｅｔａｍｏｒｐｈｏｓｉｓｏｆＰｅａｎｅｕｓｃｈｉｎｅｎｓｉｓ￥ＹｕａｎＹｏｕｘｉａｎ；ＱｕＫｅｍｉｎｇ；ＬｉｕＬｉｂｏ；ＧａｏＣ...  相似文献   

朱氏扁藻作为中国对虾幼体饵料的效果试验   总被引：1，自引：0，他引：1       下载免费PDF全文

张成武  胡鸿钧 《海洋科学》1993,17(5):38-41

本文报道了从我国海州湾沿岸海水中分离的一种易培养朱氏扁藻(Tetraselmis chui),此藻投喂中国对虾(Penaeus chinensis)幼苗,成活率可达64.30％(蚤状Ⅰ期到仔虾Ⅰ龄),与对照组同。  相似文献