应用黄金分割法优选小球藻的培养温度     

刘志媛  周俊如  崔玉兵  郑阵兵  王珺 《海洋科学》2013,37(4):37-40

利用黄金分割(0.618)优选法测定了两株分离自海南近岸海域的小球藻(Chlorella spp.)的最适生长温度。结果表明,黄金分割优选法可以较少的实验次数找到小球藻的最适培养温度。两株小球藻在15～40℃温度范围内均可生长。小球藻HNC11的最适生长温度为34～36.5℃;小球藻HNC2的最适生长温度为28～30.5℃左右。  相似文献   

Tidal flow near Mana Island,Cook Strait,New Zealand     

E. Bradford  R. A. Wooding 《新西兰海洋与淡水研究杂志》2013,47(1):31-42

Numerical calculations carried out for tidal flow on a small region of the New Zealand coast near Mana Island (41° 5 S, 174° 47 E) solved a finite difference form of the tidal equations, and assumed that the velocity boundary conditions could be approximated by a modified Kelvin wave formula. The results were exponentially smoothed in time at each step in the calculation to remove spurious modes.

The main results are shown as plots of tidal velocity vectors around Mana Island at equally spaced time intervals over a tidal cycle for one run commencing at low tide. The flow becomes very complex, with eddy shedding occurring in the wake of Mana Island, and a tidal rip developing across the shallow connection between Mana Island and the mainland.  相似文献   

Biological features of Pupu Springs     

Frances B. Mjchaelis 《新西兰海洋与淡水研究杂志》2013,47(2):357-373

The flora and fauna of Pupu Springs (40°51'S, 172° 46'E) and five other New Zealand cold springs are described. In Pupu Springs there are 16 species of algae, 10 species of bryophytes (including three species of liverworts), and 5 species of angiosperms. The fauna includes a phreatic form (an eyeless planarian, Dugesia sp.), a possible glacial relict (the caddis fly Rakiura vernale), and cold stenotherms (e.g., the caddis fly Psilochorema tautoru). The most abundant animals in the New Zealand springs are Mollusea, Crustacea, and’ larvae of Plecoptera, Diptera, and Trichoptera.

Pupu Springs consist of five biotopes and associated biocoenoses.  相似文献   

New records of distribution and co‐occurrence of freshwater calanoid copepods in New Zealand (note)     

Carolyn W. Burns 《新西兰海洋与淡水研究杂志》2013,47(1):149-150

Calamoecia lucasi was found with Boeckella propinqua in the Kaihoka Lakes, Nelson, and Boeckella hamata with Boeckella triarticulata in Lake Elterwater, Marlborough. These are the first records of C. lucasi in the South Island, New Zealand, and of B. hamata co‐occurring with B. triarticulata.  相似文献   

Algal populations and characteristics of oxygen exchange of effluent samples from a facultative oxidation pond   总被引：1，自引：1，他引：0  

D. E. Irving  F. I. Dromgoole 《新西兰海洋与淡水研究杂志》2013,47(1):9-16

Algal populations in a facultative oxidation pond were monitored over a 12 month period. The relationships between cell counts of individual species and characteristics of oxygen exchange, determined from changes in dissolved oxygen concentration in response to varying light flux density, were investigated by correlation analysis. These measurements were made in the laboratory at a constant temperature (15°C). Microalgae (Chlorella spp.) usually made up at least 80% of the total cell numbers, whereas Euglena acus, E. gracilis, and Ankistrodesmus falcatus var. acicularis separately made up less than 10% of the total counts. However, when cell volumes were considered, E. acus usually made the greatest contribution, and this species accounted for 86% (=r²) of the variation in total cell volume. The detectable pigment concentration in the water was closely related to the numbers of E. acus present (r=0.81, P <0.001) but not to the numbers of microalgae (r=0.00). Cell counts of E. acus were highly correlated with the light saturated rate of net oxygen production (P_{n max}) and to the rate under limiting light supply (6) because of the high detectable pigment contribution from this species. It was calculated that E. acus contributed, on average, about 44% of the total rate of net oxygen production, whereas the proportion attributed to the microalgae was 8%.  相似文献   

病原分离鉴定及致病性研究     

胡娇  张飞  徐晓津  苏永全  覃映雪  马英  张艺  韩坤煌  鄢庆枇 《海洋与湖沼》2014,45(2):409-417

于2013年4月从宁德患内脏白点病大黄鱼(Pseudosciaena crocea)中分离得到两株优势菌NZBD9和NZBD11, 这两株菌在16—19°C条件下回归感染能引起大黄鱼内脏白点病, 而在7—10°C和24—27°C条件下同样的人工感染不能致病, 从而证实这两株菌为大黄鱼内脏白点病的病原菌。经16S rDNA基因的测序和时间飞行质谱微生物鉴定仪分析, NZBD9 和NZBD11同为变形假单胞菌。药敏性实验结果显示NZBD9对庆大霉素、诺氟沙星和四环素等7种药物高度敏感。组织病理学观察结果显示病鱼的肝脏、脾脏、头肾等组织中均出现明显病症, 如变性和坏死。  相似文献   

Culture-dependent and independent analyses of subsurface microbial communities in oil-bearing strata of the Sagara oil reservoir   总被引：3，自引：0，他引：3  

Takuro  Nunoura  Hanako  Oida  Noriaki  Masui  Fumio  Inagaki  Ken  Takai  Satoshi  Hirano  Kenneth H.  Nealson  Koki  Horikoshi 《Island Arc》2006,15(3):328-337

Abstract   Culture-dependent and independent methods were used to evaluate the microbial communities in cores collected at depths up to 200 m in oil-bearing and oil-free strata near the Sagara oil reservoir near Shizuoka, Japan. Direct microbial counts revealed much higher numbers (2.2 × 10⁴−7.9 × 10⁶/g) of microbes in the oil-bearing strata than in the oil-free zones, where counts were uniform at approximately 1.0 × 10⁴/g. Molecular taxonomic analyses via 16S rRNA gene sequence comparisons showed that the oil-free strata were dominated by members of the γ-Proteobacteria including Pseudomonas , Stenotrophomonas and Sphingomonas , whereas the oil-bearing strata were dominated by a single species closely related to Pseudomonas stutzeri . All archaeal clones were phylogenetically affiliated with the uncultured soil group in Crenarchaeota with the exception of a single phylotype that belonged to the genus Thermococcus . Culture-dependent analysis was carried out by most-probable-number culturing as well as direct plating to determine viable cell counts, using both complex organic substrates or native oil and autotrophic media. Both culture-dependent and independent methods revealed the abundant cultivable member was the aerobic oil-degrading bacterium Pseudomonas stutzeri and neither autotrophs nor anaerobic heterotrophs could be detected in the oil-bearing strata.  相似文献   

The use of high temperature gas chromatography to study the biodegradation of high molecular weight hydrocarbons     

D. J. Heath  C. A. Lewis  S. J. Rowland 《Organic Geochemistry》1997,26(11-12)

A consequence of the biodegradation of petroleum is that lower molecular weight compounds are removed preferentially to higher molecular weight (HMW) compounds greater than triacontane (n-C₃₀). The extent to which the latter compounds are biodegraded has rarely been studied. Reasons for this include the technical difficulties associated with carrying out biodegradability tests with solid, water-insoluble substances and the limits of the analytical techniques, such as gas chromatography (GC).A quantitative high temperature GC (HTGC) method was developed to monitor the biodegradation of the aliphatic fraction of a waxy Indonesian oil by Pseudomonas fluorescens. Recoveries of over 90% were obtained for n-alkanes up to hexacontane (C₆₀) using liquid-liquid continuous extraction. After only 14 days, 80% of the aliphatic hydrocarbons had been degraded. At the end of the 136-day study, 14% of the original fraction remained. This comprised mainly C₄₀₊ compounds. No decrease in the concentrations of compounds above C₄₅ was observed. However, the use of a rapid screening biodegradation method provided tentative proof that Pseudomonas fluorescens was capable of utilising n-alkanes up to C₆₀ once the bacteria had acclimated to HMW alkanes.  相似文献   

An experimental study of the ecological impacts of hydraulic bivalve dredging on maerl     

Hauton  C; Hall-Spencer  J.M; Moore  P.G 《ICES Journal of Marine Science》2003,60(2):381-392

  相似文献   

A review of Pacific salmon hatchery programmes on Hokkaido Island, Japan     

Morita  Kentaro; Saito  Toshihiko; Miyakoshi  Yasuyuki; Fukuwaka  Masa-aki; Nagasawa  Toru; Kaeriyama  Masahide 《ICES Journal of Marine Science》2006,63(7):1353-1363

  相似文献