中国南海一株固氮类芽孢杆菌的筛选和分离鉴定   总被引：1，自引：0，他引：1  

张丽红  刘艳芳  胡青平 《海洋科学》2015,39(3):100-105

为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。  相似文献   

盐田嗜盐单胞杆菌的分离鉴定及其PHB 积累的研究     

隋丽英  张娇娇  许高超  邓元告 《海洋科学》2015,39(5):16-20

微生物聚合物聚-?-羟基丁酸酯(PHB)具有良好的生物降解性、生物相容性和热塑性,在环保、医药和农业领域具有广阔的应用前景。高PHB积累菌株的获得及其发酵条件的研究具有很好的实用价值。作者从渤海湾日晒盐场盐田卤水中分离出一株杆状细菌,经16S r DNA序列测序比对,确定为盐杆菌属(Halomonas),命名为Halomonas 100-16-2,其最适生长盐度为50~100,最适生长p H为7~8,属中度嗜盐菌。在酸水解酪蛋白(7.5 g/L)的基础培养基中分别添加0、10、20和30 g/L葡萄糖,研究盐度100条件下不同葡萄糖添加量对Halomonas 100-16-2生长和PHB含量的影响。结果表明,随着葡萄糖浓度的增加,该菌株的生长和PHB积累显著提高。葡萄糖质量浓度为30 g/L时,细胞干质量达8.60 g/L,PHB含量达到5.57 g/L(即达到细胞干质量的65%)。本研究为渤海湾盐田微生物资源的开发和利用提供了有价值的基础数据。  相似文献   

辽宁西部早白垩世朝阳翼龙科 (Chaoyangopteridae)化石一新种     

姬书安  张立军  路芳 《地质学报》2023,97(6):1723-1740

中国辽宁西部早白垩世翼龙类化石非常丰富,其上部的九佛堂组翼龙类组合以含有丰富的无齿的古神翼龙科、朝阳翼龙科等进步类型而与下部的义县组翼龙类组合相区别。本文描述了辽宁西部建昌盆地九佛堂组一新的大型翼龙类化石,其以上下颌无齿、吻端指数为3.7、前颌骨背支细长且封闭鼻眶前孔背缘、很大的鼻眶前孔后端超过上下颌关节位置、轭骨的上颌骨突基部宽大而应被归入朝阳翼龙科(Chaoyangopteridae)神州翼龙属(Shenzhoupterus)。其以较大的体形(翼展2.05 m)、平直的上下颌咬合面、轭骨的眶后骨突较泪骨突长且基部略宽、第4～7颈椎长度依次减小等特征,区别于朝阳神州翼龙(Shenzhoupterus chaoyangensis),而被命名为三亚神州翼龙(新种)(Shenzhoupterus sanyainus sp. nov.)。这是辽宁西部早白垩世翼展最大且不具牙齿的翼龙类属种,它的发现丰富了九佛堂组翼龙类组合内容,对认识朝阳翼龙科的骨骼形态与生态习性亦具有积极意义。  相似文献   

Survey of historical New Zealand shellfish samples for accumulation of gymnodimine     

David J. Stirling 《新西兰海洋与淡水研究杂志》2013,47(4):851-857

In 1994 a major biotoxin event occurred along the east coast of the South Island, New Zealand. Gymnodimine, a unique bioactive spiroimine, was isolated and characterised from Foveaux Strait dredge oysters (Tiostrea chilensis = Ostrea chilensis) collected during this outbreak. This study reports the results of liquid chromatographic‐mass spectrometric analysis for gymnodimine in 217 samples from eight species of shellfish over the years 1993–99. Of these samples 155, covering six species of shellfish, contained detectable gymnodimine with a range in concentration from 14.8 to 23 400 μg/kg. Gymnodimine‐containing shellfish occurred at 37 of the 63 sites sampled from around New Zealand. This study demonstrates that accumulation of gymnodimine is not limited to T. chilensis and can occur in other shellfish species over much of New Zealand. Gymnodimine is a possible cause of the numerous historical biotoxin screen‐positive results.  相似文献   

Population dynamics and chemical ecology of New Zealand Demospongiae Latrunculia sp. nov. and Polymastia croceus (Poecilosclerida: Latrunculiidae: Polymastiidae)     

Alan R. Duckworth  Christopher N. Battershill 《新西兰海洋与淡水研究杂志》2013,47(5):935-949

For 3 years aspects of the population dynamics, growth, and bioactivity (measure of biologically active metabolite biosynthesis) of the Demospongiae Latrunculia sp. nov. and Polymastia croceus (Kelly‐Borges & Bergquist) were examined on a subtidal reef on the Wellington south coast, New Zealand. For both species, survival of adult sponges was high in all seasons, whereas juvenile sponges had poor survival. Recruitment of Latrunculia sp. nov. occurred in all seasons indicating that this species is reproductively active throughout the year. P. croceus recruited mostly in autumn, supporting previous work that found the sponge to be reproductively active in summer and early autumn only. For both sponge species, growth rates varied greatly between individuals and were unaffected by initial sponge size within the range examined. Sponges generally grew during winter and spring as the water temperature rose, and shrank during summer and autumn as the water temperature fell. This growth pattern may relate to seasonal variation in food abundance, and for P. croceus it may result also from seasonal differences in reproductive investment. After 2 years, Latrunculia sp. nov. and P. croceus had on average, halved and doubled in size, respectively. Latrunculia sp. nov. showed a seasonal pattern of bioactivity, being most active in spring possibly to prevent the surface overgrowth of fouling organisms. P. croceus had no seasonal pattern of bioactivity, but individuals were either very active or inactive. The bioactive metabolites in both species possibly aid in competitive interactions and prevent predation and biofouling.  相似文献   

Diel patterns of mysid drift (Crustacea: Mysidacea) in the Taieri River estuary,New Zealand     

Darin L. Sutherland  Gerard P. Closs 《新西兰海洋与淡水研究杂志》2013,47(1):197-200

Drifting mysids were sampled to determine diel activity, and whether mysid numbers vary with respect to inflowing and outflowing tides. Samples of drifting mysids were collected at the mouth of the Taieri River, New Zealand, over one 24‐h cycle using five replicate conical drift nets in January 1999. One‐night and 1‐day ebb and flood tide were each sampled. Four species of mysid Tenagomysis macropsis, T. novae‐zealandiae, T. robusta, and an apparently undescribed species of Gastrosaccus sp., were collected over the sampling period. One species, T. robusta, has not previously been collected from estuarine or riverine systems. The undescribed Gastrosaccus sp. was the most abundant of the four species. All four species were more abundant at night, with T. robusta and Gastrosaccus sp. being considerably more abundant in the drift on outflowing tides. In contrast, roughly equal numbers of T. macropsis and T. novae‐zealandiae were observed drifting in and out of the estuary at night.  相似文献   

Iron and phosphorus effects on the growth of Cryptomonas sp. (Cryptophyceae) and their availability in sediments from the Pearl River Estuary, China   总被引：2，自引：0，他引：2  

Huan-Xin Weng  Ya-Chao Qin  Xiang-Wei Sun  Hailiang Dong  Xiang-Hua Chen 《Estuarine, Coastal and Shelf Science》2007,73(3-4):501-509

Based on lab-culture experiments analyzing limitation and combination of iron and phosphorus on the growth of Cryptomonas sp. (Cryptophyceae), and the study of accumulation and release of Fe-bound P in sediment cores collected from the marine region of the Pearl River Estuary, China, reasons for the high frequency of phytoplankton bloom therein are discussed. Results show that the combined effect of Fe and P can obviously accelerate algal development, and the optimum culture conditions maintaining maximum growth rate are 0.05 μM Fe and 50 μM P. Cellular contents of Fe and P is consistent and the P:Fe molar ratio is 159:1. The optimum range of the P:Fe molar ratio in culture experiments for cell incubation is 500–1400. The vertical trends of total Fe and total P variations in sediments are parallel. Fe-bound P is the main species of inorganic sedimentary P. Through continuous leaching with agitation, 34–80% of exchangeable P and 4–23% of exchangeable Fe are concurrently released from the surficial sediments. This is a possible way by which nutrients are made available to phytoplankton. These factors might be responsible for a high frequency of harmful algal blooms in the Pearl River Estuary.  相似文献   

Effects of Pseudoalteromonas sp. BC228 on digestive enzyme activity and immune response of juvenile sea cucumber (Apostichopus japonicus)     

Yuexin Ma  Feixue Sun  Congyao Zhang  Pengyun Bao  Shuqing Cao  Meiyan Zhang 《中国海洋大学学报(英文版)》2014,13(6):1061-1066

A marine bacterium, Pseudoalteromonas sp. BC228 was supplemented to feed in a feeding experiment aiming to determine its ability of enhancing the digestive enzyme activity and immune response of juvenile Apostichopus japonicus. Sea cucumber individuals were fed with the diets containing 0(control), 105, 107 and 109 CFU g-1 diet of BC228 for 45 days. Results showed that intestinal trypsin and lipase activities were significantly enhanced by 107 and 109 CFU g-1 diet of BC228 in comparison with control(P 0.01). The phagocytic activity in the coelomocytes of sea cucumber fed the diet supplemented with 107 CFU g-1 diet of BC228 was significantly higher than that of those fed control diet(P 0.05). In addition, 105 and 107 CFU g-1 diet of BC228 significantly enhanced lysozyme and phenoloxidase activities in the coelomic fluid of sea cucumber, respectively, in comparison with other diets(P 0.01). Sea cucumbers, 10 each diet, were challenged with Vibrio splendidus NB13 after 45 days of feeding. It was found that the cumulative incidence and mortality of sea cucumber fed with BC228 containing diets were lower than those of animals fed control diet. Our findings evidenced that BC228 supplemented in diets improved the digestive enzyme activity of juvenile sea cucumber, stimulated its immune response and enhanced its resistance to the infection of V. splendidus.  相似文献   

Marine bacterium strain screening and pyrethroid insecticide-degrading efficiency analysis     

孙爱丽  刘菁华  史西志  李德祥  陈炯  唐道军 《中国海洋湖沼学报》2014,32(5):1029-1035

A pyrethroid insecticide-degrading bacterium, strain HS-24, was isolated from an offshore seawater environment. The strain, which can degrade cypermethrin(CYP) and deltamethrin(DEL), was identified as Methylophaga sp. The optimal culture and degradation conditions for CYP and DEL by strain HS-24 is pH 7 at 28°C. Under optimum culture conditions, strain HS-24 exhibited a broad degradation concentration range of 100, 200, 400, 600, and 800 mg/L for CYP and DEL. The metabolic intermediates were analyzed by NMR, which provided strong evidence that CYP and DEL removal occurred mainly because of a biological process. The toxicity of the degradation products of strain HS-24 was studied simultaneously by measuring the light output of the luminescence bacterium. This demonstrated that the biodegradation ability of strain HS-24 significantly decreased the toxicity of CYP- and DEL-contaminated aquaculture seawater. Finally, the findings of this paper indicate that strain HS-24 is thus revealed as a biological agent for the remediation of marine aquatic environments.  相似文献   

温度和光径对微绿球藻生长及营养成分含量的影响     

杜晓凤  邹宁  孙东红  常林  赵萍 《海洋科学》2014,38(4):50-54

研究了温度和光生物反应器的光径对微绿球藻(Nannochloropsis oculata Hibberd)生长及细胞内几种营养成分含量的影响。结果表明,温度对微绿球藻的生长有显著影响(P0.05),对几种营养成分的含量有极显著影响(P0.01),在28℃的培养条件下,细胞生长速度最快,此时蛋白质含量最高,为5.66%,多糖含量最低,仅为1.47%,但最大生物产量却是在30℃的条件下获得,总脂与叶绿素a的含量随温度的升高而降低,类胡萝卜素含量随温度升高而增大。光生物反应器的光径对微绿球藻的生长和总脂、可溶性蛋白、色素的含量有极显著影响(P0.01),对多糖含量有显著影响(P0.05),比生长速率、最高细胞密度和单位体积产量均随光径的增大而减小,单位面积产量随光径的增大而增大;总脂、可溶性蛋白和多糖的含量随光径的增大而降低,叶绿素和类胡萝卜素含量随光径增大而增大,类胡萝卜素含量的增大趋势比叶绿素更明显。  相似文献