基于CORS网络的单频GPS实时精密单点定位新方法   总被引：3，自引：0，他引：3       下载免费PDF全文

姜卫平  邹璇  唐卫明 《地球物理学报》2012,55(5):1549-1556

利用单频接收机实现大范围、高精度快速实时定位是卫星大地测量研究的热点和难点问题之一.本文提出了一套基于连续运行参考站系统(CORS)的误差实时建模数据处理方法,设计并实现了一种新的GPS单频接收机实时精密单点定位服务方法.然后,利用山西省CORS网内一组实测动态数据,以及从湖北省CORS网和武汉市CORS网选取的一组实测静态数据进行测试,验证分析了所提出方法用于实时定位服务的可行性.结果表明:对于站间平均距离小于71 km的参考网,网内单频用户按照实时处理模式,在经过数秒钟的初始化后即可获得厘米级的动态精密单点定位结果.这为GPS单频接收机用户实现快速实时精密单点定位提供了一种可行的新方法.  相似文献   

Construction of cDNA Library from Intestine, Mesentery and Coelomocyte of Apostichopusjaponicus Selenka Infected with ~brio sp. and a Preliminary Analysis of Immunity-Related Genes     

LIU Hongzhan  ZHENG Fengrongt  SUN Xiuqin  CAI Yimei 《青岛海洋大学学报(英文版)》2012,(2):187-196

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Development of a real-time PCR method (Taqman) for rapid identification and quantification of Prorocentrum donghaiense     

Jian Yuan  Tiezhu Mi  Yu Zhen  Zhigang Yu 《中国海洋大学学报(英文版)》2012,11(3):366-374

Prorocentrum donghaiense is a dinoflagellate that is widely distributed in the East China Sea and has become increasingly involved in Harmful Algal Blooms (HABs). Therefore, it is necessary to study this dinoflagellate to monitor HABs. In this study, 13 pairs of primers specific to P. donghaiense (within its internal transcribed spacer (ITS) regions) were designed for SYBR Green I real-time PCR. As the SYBR Green I real-time PCR could not identify P. donghaiense in a specific manner, a Taqman real-time PCR method was developed by designing a set of specific primers and a Taqman probe. A 10-fold serial dilution of recombinant plasmid containing ITS regions of P. donghaiense was prepared as standard samples and the standard curve was established. Additionally, we quantified the genomic DNA in P. donghaiense cells and utilized this DNA to prepare another 10-fold serial dilution of standard sample and accordingly set up the standard curve. The mathematic correlation between the cell number and its corresponding plasmid copy number was also established. In order to test the efficiency of the real-time PCR method, laboratory samples and P. donghaiense HAB field samples were employed for identification and quantitative analysis. As to laboratory samples, as few as 102 cells of P. donghaiense could be quantified precisely utilizing both centrifugation and filtration techniques. The quantification results from field samples by real-time PCR were highly similar to those by light microscopy. In conclusion, the real-time PCR could be applied to identify and quantify P. donghaiense in HABs.  相似文献   

The Southern Ontario All-sky Meteor Camera Network     

R. J. Weryk  P. G. Brown  A. Domokos  W. N. Edwards  Z. Krzeminski  S. H. Nudds  D. L. Welch 《Earth, Moon, and Planets》2008,102(1-4):241-246

We have developed an automated network of all-sky CCD video systems to detect medium–large meteoroids ablating over Southern Ontario, Canada. The system currently consists of five stations with the largest baseline being 180 km. Each site runs a video rate recorder with sufficient resolution to determine meteoroid trajectories with a typical precision of about 300 m but no worse than 1 km. The sensitivity of the camera is close to a stellar visual magnitude of +1 which allows for astrometric calibrations using field stars. Photometric procedures have also been developed and tested. The system has a limiting magnitude for meteors of about −2 with the current detection algorithm.  相似文献   

泥鳅(Misgurnus anguillicaudatus)病原霍乱弧菌(Vibrio cholerae)PCR 与LAMP检测方法的比较研究     

张晓君  白雪松  毕可然  许加涛  秦蕾  阎斌伦 《海洋与湖沼》2013,44(1):209-214

采用分子生物学方法,以霍乱弧菌lolB为靶基因设计特异性引物,进行了霍乱弧菌的PCR和环介导等温核酸扩增(Loop-mediated isothermal amplification,LAMP)检测技术研究,并对它们的特异性、灵敏性和实际应用进行了比较.结果表明,所建立的PCR检测霍乱弧菌的方法最低检测限为4.0× 103 CFU/ml; LAMP检测方法在65℃下恒温扩增60min,检测限为4.0×101 CFU/ml,反应产物加入荧光染料SYBR Green Ⅰ后反应液呈现明显的绿色;以温和气单胞菌、副溶血弧菌、鳗弧菌及美人鱼弧菌为对照菌株,检测结果均为阴性;霍乱弧菌人工染菌的8种水产品进行PCR及LAMP检测,结果均为阳性,而未染菌组均为阴性;PCR及LAMP检测霍乱弧菌的方法均具有灵敏度较高、特异性强等优点,且LAMP检测霍乱弧菌的方法灵敏度是PCR方法的100倍,更适合于养殖现场检测的推广使用.  相似文献   

大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因的分子克隆和表达特征分析     

韦秀梅  杨顶珑  杨建敏  杨嘉龙  徐洁  刘相全  张艳敏 《海洋与湖沼》2013,44(3):584-589

本研究克隆得到了一个大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因(SgTCTP)的cDNA全长,其序列全长为1055bp,5′和3′端的非编码区(UTR)分别为54和461bp,开放阅读框(ORF)540bp,编码179个氨基酸,理论等电点为4.50,预测分子量大小19.96kDa。通过荧光定量PCR法检测了SgTCTP在健康大竹蛏各组织中和病原相关分子模式(PAMPs)刺激后的表达规律,结果表明:SgTCTP在检测组织外套膜、鳃、性腺、血细胞、肌肉和肝胰腺中都有表达,其中在肝胰腺中的表达量最高。脂多糖(LPS)、肽聚糖(PGN)和葡聚糖(β-1,3-glucan)刺激都能诱导SgTCTP的表达量上调,SgTCTP的表达量分别在LPS和PGN刺激后6和3h达到最高,为空白对照的3.64和3.36倍;β-1,3-glucan刺激后SgTCTP的表达量上升幅度最大,在12h达到最高,为空白对照的11.76倍。SgTCTP可能作为急性时相蛋白参与大竹蛏的免疫应答。  相似文献   

Non-destructive sampling of juvenile abalone using epipodial tentacles and mucus: method and application     

《African Journal of Marine Science》2013,35(3-4):719-721

Good-quality biological material is needed to obtain intact deoxyribonucleic acid (DNA) for use in molecular techniques such as the polymerase chain reaction (PCR). Non-destructive sampling protocols of juvenile abalone Haliotis midae (7–15 months old) were tested in order to collect material for DNA extraction. DNA was successfully extracted from epipodial tentacles and mucus samples. PCR results confirmed the good quality of the DNA and the reliability of the method.  相似文献   

Experimental test of asymmetrical cable-stayed bridges using MR-damper for vibration control     

《Soil Dynamics and Earthquake Engineering》2014

In this paper, a semi-active control by MR-damper is researched; its purpose is to effectively control vibration of asymmetrical cable-stayed bridges when earthquake is loaded on the type of bridge. For an experimental study, a model of 10.2 m high and 28 m long asymmetrical cable-stayed bridge structure was built being similar to a real one in size and function. A MR damper was also designed in proper size suitable for the control of the model. The experiment was performed in the way in which three piers were fixed on three shaking tables with 30% of El-centro earthquake wave, and a control device was placed on the lower part of its upper deck for horizontal control. As for control algorithms, Lyapunov and Clipped-optimal control algorithms were applied. The effectiveness of the semi-active control with MR damper for the asymmetrical cable-stayed bridge was measured under five control conditions: Un-control, Passive-off, Passive-on, Lyapunov Control, Clipped-optimal Control. The experiment showed that the semi-active control applying Lyapunov and Clipped-optimal algorithms effectively increased controllability almost in double, and decreased displacement 75% compared with the condition of passive-off. Therefore the semi-active method suggested in this paper is proven effective in controlling asymmetrical cable-stayed bridges.  相似文献   

X射线极光强度与亚暴指数AE的相关关系          下载免费PDF全文

赵亮  涂传诒 《地球物理学报》2005,48(4):739-743

研究了Polar卫星的极区电离层X射线成像仪（PIXIE）得到的极光X射线成像强度AI（Auroral Intensity）与磁层亚暴指数的相关关系.本文发现,在所选取的1997年至2001年的部分数据中,从完整的X射线图像得到的极光X射线总强度和AE指数有很好的线性相关关系,在全部83组数据中有566％的数据的线性相关系数都在060以上（相关系数最大为097）.所以本文认为极光X射线总强度可以作为新的磁层亚暴卫星实时监测指数.  相似文献   

迟缓型爱德华氏菌(Edwardsiella tarda)诱导牙鲆(Paralichthys olivaceus)TLR1及TLR2基因的表达分析     

李庆亚  周密  张洁  郑津辉  耿绪云  潘宝平  孙金生  高虹 《海洋与湖沼》2017,48(4):848-856

Toll样受体是一类重要的蛋白质分子,参与固有免疫系统,在哺乳动物在受到细菌感染的时候,TLR1和TLR2基因可以形成异源二聚体,进而启动宿主的固有免疫。本文应用实时荧光定量PCR的技术,检测了TLR1和TLR2基因在牙鲆健康组织以及牙鲆腹腔注射迟缓型爱德华氏菌(Edwardsiella tarda)后各组织中的表达变化,并探讨了它们与牙鲆(Paralichthys olivaceus)固有免疫反应的关系。结果表明,TLR1和TLR2基因广泛表达于健康牙鲆的各种组织中,其中,TLR1在脾脏组织中表达量最高,其次是心脏、肌肉;TLR2在小肠组织中表达量最高,其次是肝脏、心脏。免疫刺激实验表明,多数组织在感染病原6h后TLR1基因表达达到峰值,其中脾脏中基因的表达量最大,是0时间点的290倍(P0.01)。TLR2基因在感染病原1h后在脾脏中表达量最高,为0时间点的17.8倍(P0.01),在感染病原1d后心脏组织中基因的表达量为对照组的14倍(P0.01),其余时间点表达变化不明显。结果表明TLR1和TLR2参与了牙鲆对迟缓型爱德华氏菌的免疫应答反应。实验结果还显示,在牙鲆感染迟缓型爱德华氏菌后,MyD88、TNF-α和IL-1基因的表达也都同步上调,暗示迟缓型爱德华氏菌有可能通过TLR1通路上调MyD88的表达,并最终导致炎症因子TNF-α和IL-1的基因表达上调,以应答病原菌的感染。  相似文献