一种水产动物病毒现场检测免疫芯片的制备与应用   总被引：4，自引：2，他引：2  

徐晓丽  绳秀珍  战文斌 《海洋与湖沼》2010,41(5):698-706

采用原子力显微镜对氨基化玻片及6种不同方法修饰玻片进行表面特征分析,并比较了硝酸纤维素(NC)膜、PVDF膜以及以上不同修饰玻片对抗体的固定效率、固定效果,最终选择琼脂糖修饰玻片作为芯片载体。将纯化后的兔抗血清(捕获抗体)点样至琼脂糖修饰玻片上,制备免疫芯片,与待检样品(病毒感染的靶器官组织)匀浆液孵育形成复合物,该复合物被辣根过氧化物酶(HRP)标记的特异性单克隆抗体(单抗)识别,经底物显色,得到肉眼可见的检测结果。通过改变芯片制备及应用过程中的具体条件参数,对各条件进行了优化,并采用生物素-链亲和素(BAS)标记特异性单抗作为检测抗体以提高检测灵敏度。基于此夹心免疫分析原理制备的WSSV、LCDV现场检测免疫芯片,病毒的最低检出量分别为82.50ng/ml、0.88μg/ml,在一定的抗原浓度范围内,病毒浓度的对数值与信号强度呈线性关系;采用BAS放大检测信号后,病毒的最低检出量为12.38ng/ml、0.22μg/ml。该免疫芯片与酶联免疫吸附法(ELISA)及免疫荧光法(IFAT)对同种样品的检测结果高度一致。  相似文献   

虾蟹混养塘中WSSV对三疣梭子蟹(Portunus trituberculatus)致死效应的定量研究   总被引：1，自引：0，他引：1  

王忠发  王志铮  许文军  何伟贤  顾松叶 《海洋与湖沼》2008,39(2):184-189

采用实时荧光定量PCR法,在对白斑综合症多发的虾蟹混养塘内三疣梭子蟹体内病毒含量进行定量跟踪检测的基础上,结合电子显微镜及病理切片,对室内经口服、注射两种回感模式处理已感染白斑综合症病毒的濒死三疣梭子蟹体内病毒含量进行实验验证,并观察其体内主要器官组织的病理改变。结果表明,受白斑综合症病毒感染的三疣梭子蟹存活与死亡取决于蟹体内病毒含量,当病毒含量≥109拷贝/ml时均进入濒死或死亡阶段,其主要器官组织均有明显的病理改变。  相似文献   

Testing energy non-additivity in white dwarfs     

《Astroparticle Physics》2014

We consider a particular effect which can be expected in scenarios of deviations from special relativity induced by Planckian physics: the loss of additivity in the total energy of a system of particles. We argue about the necessity to introduce a length scale to control the effects of non-additivity for macroscopic objects and consider white dwarfs as an appropriate laboratory to test this kind of new physics. We study the sensitivity of the mass-radius relation of the Chandrasekhar model to these corrections by comparing the output of a simple phenomenological model to observational data of white dwarfs.  相似文献   

An upper limit to coronal X-rays from single,magnetic white dwarfs     

Rob Cavallo  Keith A. Arnaud  Virginia Trimble 《Journal of Astrophysics and Astronomy》1993,14(3-4):141-143

Pointed ROSAT PSPC exposures of 9277 and 6992 sec, directed toward the nearby, single, cool, magnetic white dwarfs GR 290 and EG 250 yielded no counts significantly above the expected background rate. The corresponding flux limits (for an assumed source temperature of l keV) are 1.0 and 1.7 × 10⁻¹⁴erg cm⁻² s⁻¹, within the 0.1–2.5 keV bandpass of the instrument (99% confidence limits). This is more than an order of magnitude below the tentative detection level (for GR 290) and limits (for four other similar stars) obtained from archival Einstein data in 1991. The corresponding limits on coronal electron density are comparable with those implied if cyclotron emission is not responsible for any of the features observed in the optical spectra of magnetic white dwarfs. X-ray data currently provide no evidence for the existence of coronae around these stars. A final long observation (25,000 sec of GD 356) is scheduled for later this year on ROSAT, along with coordinated EUVE observations.  相似文献   

1989年1月18日白光耀斑的研究     

《天文研究与技术》1996,(1)

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Using cryptotephras to extend regional tephrochronologies: An example from southeast Alaska and implications for hazard assessment     

Richard Payne  Jeffrey Blackford 《Quaternary Research》2008,69(1):42-55

Cryptotephrochronology, the use of hidden, diminutive volcanic ash layers to date sediments, has rarely been applied outside western Europe but has the potential to improve the tephrochronology of other regions of the world. Here we present the first comprehensive cryptotephra study in Alaska. Cores were extracted from five peatland sites, with cryptotephras located by ashing and microscopy and their glass geochemistry examined using electron probe microanalysis. Glass geochemical data from nine tephras were compared between sites and with data from previous Alaskan tephra studies. One tephra present in all the cores is believed to represent a previously unidentified eruption of Mt. Churchill and is named here as the ‘Lena tephra’. A mid-Holocene tephra in one site is very similar to Aniakchak tephra and most likely represents a previously unidentified Aniakchak eruption, ca. 5300-5030 cal yr BP. Other tephras are from the late Holocene White River eruption, a mid-Holocene Mt. Churchill eruption, and possibly eruptions of Redoubt and Augustine volcanoes. These results show the potential of cryptotephras to expand the geographic limits of tephrochronology and demonstrate that Mt. Churchill has been more active in the Holocene than previously appreciated. This finding may necessitate reassessment of volcanic hazards in the region.  相似文献   

地面γ能谱测量中连续测量的理论基础和实验结果     

贾文懿  葛君伟 《地球物理学报》1991,34(4):527-530

在物化探方法中,地面γ能谱测量过去主要用于大比例尺异常检查,采用的是定点测量方式。理论分析表明,如果将其应用于1:5万地质填图,这种测量方式取样代表性差,工效较低,不能满足地质工作的要求。为了解决这一问题,充分发挥地面γ能谱测量的作用,本文提出了步行连续测量方式。 步行连续测量就是将探测器提在手上或背在身上,沿测线以一定速度边走边测的工作方式。  相似文献   

连县小带铅锌铁锰矿床特征与喷流沉积机制浅析   总被引：1，自引：0，他引：1  

郑庆年 《矿产与地质》1992,6(2):111-117

连县小带是粤北近几年发现的又一喷流沉积铅锌矿床。简要介绍其成矿环境、地质特征,讨论喷流(喷气)沉积(成矿)作用及成矿分带的主要地球化学机制,如因脉动喷流(喷气)造成盆地内地球化学性质的周期变化,引起矿石组构的周期变化等。  相似文献   

对虾白斑综合症病毒病围栏封闭预防技术研究简报   总被引：6，自引：1，他引：6  

李德尚  董双林 《海洋科学》2002,26(4):67-68

病毒性流行病使世界的养虾业遭受到了严重挫折 ,其中又以白斑综合症病毒 (ＷＳＳＶ)病流行最广 ,危害最大。针对这种病的病原、病理和流行病学已进行了大量的研究 ,对于如何预防也做了很多探索 ,但迄今没有得到很好的解决。养虾界总结出高位池塘养殖、内地淡化养殖、远海岸卤水、井水或渗水养殖和虾池配养肉食性鱼类等办法 ,都有一定的效果。但这些办法大多要求一定的条件 ,缺乏普适性 ,而且往往有时效限制 (即养殖年久后也会发病 ) ;有的则只是减小了发病机率 ,并不是十分可靠 (如配养肉食性鱼类 )。因此 ,研制出一种具有普适性 (特别是能…  相似文献   

Properties of fluids attending variable recrystallization of quartzite during contact metamorphism in the White‐Inyo Range,California          下载免费PDF全文

P. I. Nabelek  S. K. Stephenson  S. S. Morgan  J. J. Student 《Journal of Metamorphic Geology》2017,35(3):357-369

Fluid inclusions in the metamorphic aureole of the Eureka Valley‐Joshua Flat‐Beer Creek (EJB) pluton in the White‐Inyo Range, California, reveal the compositions and origin of fluids that were present during variable recrystallization of quartzite with sedimentary grain shapes to metaquartzite with granoblastic texture. Metamorphosed sedimentary formations, including quartzites, marbles, calcsilicates and schists, became ductile and strongly attenuated in the aureole during growth of the magma chamber. The microstructures of quartzites have an unusual distribution in that within ~250 m from the pluton, where temperatures exceeded 650 °C, they exhibit relict sedimentary grain shapes, only small amount of grain boundary migration (GBM), and crystallographic preferred orientations (CPOs) dominated by <a> slip. At distances >250 m, quartzites were completely recrystallized by GBM and CPOs are indicative of prism [c] slip, characteristics that are typically associated with H₂O‐assisted, high‐T recrystallization. The lack of extensive GBM in the inner aureole can be attributed to rapid replacement of H₂O by CO₂ produced by reaction of quartz grains with calcite cement that also produced interstitial wollastonite. Fluid inclusions in the inner aureole generally occur in margins of quartz grains and are either wholly aqueous (Type 1) or also contain H₂S, CO₂ and CH₄ (Type 2). Type 2 inclusions occur only in some stratigraphic layers. In both inclusion types, NaCl and CaCl₂, in variable proportions, dominate the solutes in the aqueous phase, whereas FeCl₂ and KCl are less abundant solutes. The solutes indicate attainment of a degree of equilibrium with carbonates and schists that are interbedded with the quartzites. Some Types 1 and 2 inclusions in the inner aureole show evidence of decrepitation due to high amounts of strain and/or heating suffered by the host rocks, which suggests that they represent pore fluids that existed in the rocks prior to contact metamorphism. In addition to Type 1 inclusions, outer aureole quartzites also contain inclusions that contain CO₂ vapour bubbles in addition to aqueous phase (Type 3). These inclusions only occur in interiors of granoblastic quartz that was produced by large amounts of GBM. The aqueous phase has identical ranges of first melting and final ice melting temperatures as Type 1 inclusions, suggesting that they have the same solute compositions. These inclusions are thought to represent the interstitial pore H₂O that promoted recrystallization of quartz and reacted with graphite to produce CO₂. Absence of significant amounts of CH₄ in Type 3 inclusions is attributed to elevated fO₂ that was buffered by mineral assemblages in interbedded schists. As opposed to the large amount of CO₂ that was produced by the wollastonite‐forming reaction in the inner aureole to inhibit GBM, the amount of CO₂ produced in the outer aureole by reaction between H₂O and graphite was apparently insufficient to inhibit recrystallization of quartz.  相似文献