Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

3种蛏类线粒体16SrRNA和COI基因片段的序列比较及其系统学初步研究   总被引：15，自引：0，他引：15  

陈丽梅  孔晓瑜  喻子牛  于珊珊  徐晖 《海洋科学》2005,29(8):27-32

对3种蛏类大竹蛏（Solen grandis），长竹蛏（Solen strictus）和小刀蛏（Cultellus attenuatus）的线粒体16SrRNA和COI基因片段序列进行了比较并对其系统学进行了初步研究。得到的序列总长度分别为472-481bp（16S）和658bp（COI）。3种蛏序列的碱基组成均显示出较高的A＋T比例（16SrRNA基因62．1％；COI基因62．8％）。对位排序比较表明，16SrRNA片段种内个体间变异较小，3种类间存在128个碱基变异位点（其中包括127个简约信息位点）和5个插入／缺失位点，总共12个碱基长；COI片段有200个碱基存在变异，其中包括191个简约信息位点，不存在任何插入／缺失位点。数据分析结果表明16SrRNA和COI基因片段大竹蛏与长竹蛏两片段的遗传距离分别为0．0856和0．1712，两竹蛏类与小刀蛏的遗传距离分别为0．3054，0．2798和0．2662，0．2933。作者认为小刀蛏与竹蛏之间的遗传距离已达到科之间的水平，结果支持将其提升为刀蛏科的分类观点。3种蛏类线粒体16SrRNA和COI基因在种间明显的多态性，证实了16SrRNA和COI基因序列均普遍适用于蛏类种及以上阶元的系统学分析。  相似文献   

Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay     

Taban IC  Bechmann RK  Torgrimsen S  Baussant T  Sanni S 《Marine environmental research》2004,58(2-5):701-705

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.  相似文献   

Molecular identification of ascaridoid nematodes from the deep-sea onion-eye grenadier (Macrourus berglax) from the East Greenland Sea   总被引：2，自引：0，他引：2  

Esra Kellermanns  Sven Klimpel  Harry Wilhelm Palm 《Deep Sea Research Part I: Oceanographic Research Papers》2007,54(12):2194-2202

Macrourus berglax from the East Greenland Sea was studied for the presence of ascaridoid nematodes in 2001, 2002 and 2003. The fishes were collected between 278 and 413 m water depth using a benthopelagic net. Based on the amplification of the internal transcribed spacer ITS-1, 5.8S, ITS-2 and flanking sequences (=ITS+), three ascaridoid nematode species were identified. The prevalence of infestation during the 3 years ranged from 42.9% to 62.9% and 22.9% to 40.0% for the anisakids Anisakis simplex (s.s.) and Pseudoterranova decipiens (s.s.), respectively, and from 28.6% to 60.0% for the raphidascarid Hysterothylacium aduncum. A total of 18 specimens, two of each species and examination year, revealed no sibling species, suggesting a limited distribution of other ascaridoid siblings into the deep sea. The ITS-1, 5.8S and ITS-2 sequences of A. simplex (s.s.) from the East Greenland Sea did not differ from previously published sequence data (GenBank) from other regions in the Atlantic and Pacific oceans. The sequences of P. decipiens (s.s.) corresponded most closely to those of specimens from Richardson Bay, western Pacific, and differed in four positions (0.5%). They corresponded least to those of specimens from Japan (1.5%). The sequence data for H. aduncum differed in two positions in the ITS-1 (0.2%) and three positions in the ITS-2 (0.3%) from sequences from Japan. A high genetic similarity between the regions can be explained by (a) extensive final host migration in the case of A. simplex (s.s.), (b) an overlapping distribution of final host populations along the continental shelves for P. decipiens (s.s.) and (c) a low host specificity and large population size in the intermediate and final hosts for H. aduncum. The occurrence of the identified species in the macrourid fish underlines the potential of cosmopolitan ascaridoid nematodes to distribute not only horizontally but also vertically in the deep sea.  相似文献   

Rapport ARN/ADN et evaluation de l'etat nutritionnel et de la croissance des larves de poissons marins: un essai de mise au point experimentale chez la sole (Solea solea L.)     

Bergeron  Jean-Pierre; Boulhic  Michel 《ICES Journal of Marine Science》1994,51(2):181-190

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UNB多波束数据格式解析     

王崇明  王泽民  李建虎 《测绘信息与工程》2008,33(3):13-14

采用VB进行程序设计,实现了对数据文件的解析,提取出了各种不同类型的数据,介绍了数据的处理与应用.  相似文献   

汶川地震山地灾害遥感快速提取及其分布特点分析   总被引：12，自引：0，他引：12  

苏凤环  刘洪江  韩用顺 《遥感学报》2008,12(6)

针对泥石流滑坡灾害体特有的物质组成与活动特点,通过比较分析其在不同遥感影像的光谱特征差异,选择ETM+影像作为主要数据源,提取湿度指数与绿度指数,利用ETM+的穗帽变换、影像差值增强、密度分割和掩膜技术建立了泥石流滑坡山地灾害快速提取模型,并用于汶川地震.通过灾害体的提取,分析了本次地震山地次生灾害的分布规律,利用空间叠加进行了成因的分析.本次地震山地灾害具有如下特点:(1)沿主要地表破裂带分布;(2)山地灾害主要出现在8度-9度地震烈度区,随着烈度的降低,山地灾害的总面积也相应的减少;(3)山地灾害主要发生在海拔高度1000-2500m的地带;(4)主要发生在坡度20°-50°之间的边坡上;(5)地震及余震期间以崩塌滑坡滚石为主,后期以泥石流滑坡为主;(6)具有河流左右两岸呈不对称分布等特点.结果表明,利用ETM+影像建立基于湿度指数与绿度指数的快速提取模型,对于大规模泥石流滑坡提取效果较好,进行大区域山地灾害的遥感快速提取是可行的.  相似文献   

遥感影像特征提取与选择及在影像分类中的应用     

秦永  宋伟东 《测绘科学》2008,33(1):176-178,199

利用模式识别中特征提取和特征选择的相关理论对遥感影像的纹理特征进行遴选和变换处理,得到描述纹理的二次特征。实验证明这些新特征能够提高影像分类精度和分类效率。  相似文献   

遥感图像线性影纹理解专家系统设计与实现     

李百寿  秦其明  许军强  张万峰  姚云军 《测绘科学》2008,33(2):167-169,109

线性影纹信息是遥感图像中的一类重要信息,线性影纹信息自动提取是遥感图像智能解译的重要研究领域。笔者采用基于特征对象的专家系统技术来完成线性影纹信息的简单类别提取,设计并实现了线性影纹理解专家系统;阐明了系统结构,解译规则获取、表达,基于消息的不确定性推理机及解释机的设计;最后利用系统做了线性影纹类别提取实验,结果表明基于特征对象的专家系统用于线性影纹类别自动提取是切实可行的,并具有较高的提取精度。  相似文献   

Populating a building Multi Representation Data Base with photogrammetric tools: Recent progress     

Benoit Frdricque  Sylvie Daniel  Yvan Bdard  Nicolas Paparoditis 《ISPRS Journal of Photogrammetry and Remote Sensing》2008,63(4):441-460

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