对虾白斑综合症病毒极早期基因启动子筛选文库的构建     

林梵宇  徐丽美  杨丰 《台湾海峡》2010,29(2):184-188

对虾白斑综合症病毒（white spot syndrome virus,WSSV）是危害对虾养殖业的主要病原,而WSSV极早期基因对病毒功能基因的转录翻译和调控起着至关重要的作用.本研究将WSSV基因组以超声法随机断裂至400～900bp DNA片段,并插入启动子缺失且多克隆位点下游具有报告基因的载体来构建文库,转染昆虫细胞Hi5.倒置荧光显微镜观察发现部分转染后的细胞有增强型绿色荧光蛋白（EGFP）的表达,表明这部分细胞中的DNA片段可能具有极早期启动子活性.流式细胞仪分析显示,这部分荧光细胞占总细胞的比例为0.35%.由于转染效率为50%,可认为占比0.7%的WSSV基因组随机片段能在Hi5细胞中激发下游报告基因的转录翻译,可能具有极早期基因启动子活性.这对于进一步筛选和鉴定WSSV极早期基因及开展极早期启动子研究具有重要的意义.  相似文献   

Differential gene expression in the brain of Sebastiscus marmoratus in response to exposure to polychlorinated biphenyls (PCBs)     

Li B  Wang C  Ye K  Yu A  Chen Y  Zuo Z 《Marine environmental research》2008,66(5):548-552

Effects of exposure to polychlorinated biphenyls (PCBs) on Sebastiscus marmoratus were investigated using a suppression subtractive hybridization method. A total of 108 gene sequences were identified as having the potential for being differentially expressed, and 45 could be identified with homologous database sequences. Functions with which they were associated included long-term potentiation and neurotransmitter release, neuroendocrine, mitosis and cell proliferation, energy-related metabolism, general metabolism, signal protein, hemopoiesis system, immune system, and structure. The expression of 17 of these genes was analyzed in the brain using real time fluorescent quantitative PCR. The present study provided a basis for studying the response of fish to PCB exposure and allowed the characterization of new potential neurotoxicol biomarkers of PCB contamination in seawater.  相似文献   

ROBUST: The ROle of BUffering capacities in STabilising coastal lagoon ecosystems     

Rutger de Wit  Lucas J. Stal  Bente Aa. Lomstein  Rodney A. Herbert  Hans van Gemerden  Pierluigi Viaroli  Victor-Ugo Cecherelli  Francisco Rodríguez-Valera  Marco Bartoli  Gianmarco Giordani  Roberta Azzoni  Bart Schaub  David T. Welsh  Andrew Donnelly  Ana Cifuentes  Josefa Antn  Kai Finster  Lise B. Nielsen  Anne-Grethe Underlien Pedersen  Anne Turi Neubauer  Marina A. Colangelo  Sander K. Heijs 《Continental Shelf Research》2001,21(18-19)

“Buffer capacities” has been defined in ecology as a holistic concept (e.g., Integration of Ecosystem Theories: A Pattern, second ed. Kluwer, Dordrecht, 1997, 388pp), but we show that it can also be worked out in mechanistic studies. Our mechanistic approach highlights that “buffering capacities” can be depleted progressively, and, therefore, we make a distinction between current and potential “buffering capacities”. We have applied this concept to understand the limited “local stability” in seagrass ecosystems and their vulnerability towards structural changes into macro-algal dominated communities. We explored the following processes and studied how they confer buffering capacities to the seagrass ecosystem: (i) net autotrophy is persistent in Zostera noltii meadows where plant assimilation acts as a sink for nutrients, this contrasted with the Ulva system that shifted back and forth between net autotrophy and net heterotrophy; (ii) the Z. noltii ecosystem possesses a certain albeit rather limited capacity to modify the balance between nitrogen fixation and denitrification, i.e., it was found that in situ nitrogen fixation always exceeded denitrification; (iii) the nitrogen demand of organoheterotrophic bacteria in the sediment results in nitrogen retention of N in the sediment and hence a buffer against release of nitrogen compounds from sediments, (iv) habitat diversification in seagrass meadows provides shelter for meiofauna and hence buffering against adverse conditions, (v) sedimentary iron provides a buffer against noxious sulfide (note: bacterial sulfide production is enhanced in anoxic sediment niches by increased organic matter loading). On the other hand, in the coastal system we studied, sedimentary iron appears less important as a redox-coupled buffer system against phosphate loading. This is because most inorganic phosphate is bound to calcium rather than to iron. In addition, our studies have highlighted the importance of plant–microbe interactions in the seagrass meadows.  相似文献   

激光SLAM精度测试场设计与实现     

杨啸天  李广云  向奉卓  王力  罗豪龙 《测绘通报》2020,(5):64-68

同时定位与地图构建（SLAM）系统在机器人、自动驾驶和移动测量等领域有广泛的应用，但目前系统精度的测试方法尚不完善。针对SLAM系统精度测试的实际需求，本文提出了一种SLAM高精度测试场的建立方法。首先建立三维控制网，然后利用三维激光扫描技术对环境信息进行采集，最终构建出用于测试SLAM系统精度的高精度三维点云特征库。试验结果表明，建立的三维点云特征库包含丰富的特征信息，具有真实地理坐标，精度达到毫米级，可以较好地满足SLAM精度测试的需求。  相似文献   

Cloning and Characterization of an Abalone （Haliotis discus hannai ） Actin Gene   总被引：2，自引：0，他引：2  

MAHongming XUWei MAIKangsen LIUFUZhiguo CHENHong 《中国海洋大学学报(英文版)》2004,3(2):145-149

1　Introduction　Actinisanimportantcontractileproteinineukary oticcells .Itisoneofthetwomajorcomponentsin volvedinthecontractionofmusclecells .Innon mus clecells ,itisthemajorpartofcytoskeletoninvolvedinmanyprocessessuchascellmotility ,endocytosis ,exocyt…  相似文献   

基于GDI+的高精度地图符号库的设计与实现   总被引：8，自引：1，他引：8  

吴小芳  徐智勇  蔡忠亮  杜清运 《武汉大学学报(信息科学版)》2004,29(10):928-932

通过对地图符号的数据结构进行分析，设计出满足地图需求的高精度地图符号，方便地图符号库的管理与维护。此外，考虑到线型符号绘制中的速度慢的特点，提出了高效率的绘制方法，设计并实现了一个较好的地图符号库系统。  相似文献   

插件技术在天气雷达数据处理软件中的应用   总被引：4，自引：2，他引：4  

沃伟峰  吴蕾  顾松山 《南京气象学院学报》2004,27(6):806-813

介绍了在多普勒天气雷达二次产品软件的编写中使用动态链接库(Dynam—ic—link Library)技术设计的一个插件系统(Plug—in System)。该系统中所有的算法都设计成插件，将产品算法部分和系统本身分离，便于工程的管理和进一步开发，使研究人员能够集中精力致力于算法的实现。该系统本身也可以作为一个新算法、新产品的开发平台。  相似文献   

Molecular cloning and mRNA expression of cytochrome P4501A1 and 1A2 in the liver of common minke whales (Balaenoptera acutorostrata)     

Satoko Niimi  Michio X. Watanabe  Eun-Young Kim  Hisato Iwata  Genta Yasunaga  Yoshihiro Fujise  Shinsuke Tanabe 《Marine pollution bulletin》2005,51(8-12):784

This study presents full-length cDNA sequences of CYP1A1 and 1A2, in common minke whale (Balaenoptera acutorostrata) from the North Pacific. Both CYP1A1 and CYP1A2 cDNAs had an open reading frame of 516 amino acid residues, and predicted molecular masses were 58.3 kDa and 58.1 kDa, respectively. The deduced full-length amino acid sequence of CYP1A1 revealed higher identities with those of sheep (86%) and pig (87%), and that of CYP1A2 was most closely related to human (82%) and monkey CYP1A2 (82%) among species from which CYP1A2 has been isolated so far. Differences in certain conserved and functional amino acid residues of CYP1A1 and 1A2 between common minke whale and other mammalian species indicate the possibility of their specific metabolic function. Concentrations of organochlorine compounds (OCs) including PCBs and DDTs analyzed in common minke whale liver showed no significant correlation with hepatic mRNA expression levels of CYP1A1 and CYP1A2, indicating no induction of these enzymes by such OCs.  相似文献   

基于ArcGIS Engine的地图符号制作工具的设计实现   总被引：3，自引：1，他引：3  

张俊岭  熊卫东  夏斌 《测绘信息与工程》2006,31(5):43-44

分析了ArcGIS Engine符号体系特点,设计了一套基于ArcGIS Engine的符号制作工具,采用C#语言开发实现。  相似文献   

浅谈中小型专业图书馆期刊文献资源开发利用     

李文光  王莹 《化工矿产地质》1998,20(3):254-258,264

为搞好对地质专业图书馆科技期刊收藏和更充分地开发利用科技期刊文献资源,图书馆工作人员需努力提高业务素质;努力做到合理配置,深入课题为读者服务,同时开展多样化服务,拓宽科技期刊文献资源利用领域。  相似文献