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111.
112.
Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis) 总被引:3,自引:1,他引:2
A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus. 相似文献
113.
In this work, enhanced poly(3-hexylthiophene):[6,6]-phenyl-C61-butyric acid methyl ester (P3HT:PCBM) bulk-heterojunction photovoltaic devices are achieved via slow-solvent-vapour treatment. The correlations between the morphology of the active layer and the photovoltaic performance of polymer-based solar cell are investigated. The active layers are characterized by atomic force microscopy and optical absorption. The results show that slow-solvent-vapour treatment can induce P3HT self-organization into an ordered structure, leading to the enhanced absorption and efficient charge transport. 相似文献
114.
We describe the microfabrication of 85Rb vapour cells using a glass-silicon anodic bonding technique and in situ chemical reaction between rubidium chloride and barium azide to produce Rb. Under controlled conditions, the pure metallic Rb drops and buffer gases were obtained in the cells with a few mm3 internal volumes during the cell sealing process. At an ambient temperature of 90 ℃ the optical absorption resonance of 85Rb D1 transition with proper broadening and the corresponding coherent population trapping (CPT) resonance, with a signal contrast of 1.5% and linewidth of about 1.7 kHz, have been detected. The sealing quality and the stability of the cells have also been demonstrated experimentally by using the helium leaking detection and the after-9-month optoelectronics measurement which shows a similar CPT signal as its original status. In addition, the physics package of chip-scale atomic clock (CSAC) based on the cell was realized. The measured frequency stability of the physics package can reach to 2.1×10-10 at one second when the cell was heated to 100 ℃ which proved that the cell has the quality to be used in portable and battery-operated devices. 相似文献
115.
本文依据大量测试数据,对金堆城地区花岗岩类主要造岩矿物学特征同钼迁移富集成矿关系进行了探讨。研究结果表明:异常晶胞条纹长石和正长石以及富重稀土和具明显负铕异常的黑云母是含钼花岗岩类标型矿物,并且可作为花岗岩类岩石发育地区寻找隐伏钼矿体的指示性矿物。钾钠长石和斜长石的结构状态和成分不仅可以提供有关花岗岩类成因演化的信息,其矿物本身亦可成为钼的载体矿物。岩浆期后,强烈的热液蚀变作用可造成钾钠长石和斜长石分解,并且引起钼的释放,从而形成高品位钼矿石。 相似文献
116.
本文提出了自然金-银类质同象系列矿物的晶胞参数与金、银原子数百分比成二次函数关系,推导出了表示二者之间函数关系的方程式。利用该方程式,可以用金原子数百分比计算出晶胞参数,反之亦然。并且从金、银二者的电负性出发,对a值的变化作了解释。 相似文献
117.
麻粒岩区构造地质编图原则探讨 总被引:2,自引:0,他引:2
麻粒岩区的边界及亚区划分标志常为等变线、断层或剪切带。主要地质单元包括灰色片麻岩、花岗片麻岩、混合岩、副变质表壳岩、层状侵入体、高级变质绿岩等,基性岩墙群为划分不同地质单元、建立其相对时序的重要标志。以上单元普遍被构造叠置,常可识别出侵入关系。表壳岩系底部不整合及地层层序的研究对于认识大陆克拉通化过程及沉积环境演化具有重要意义。早期大规模水平构造先后被两类变形序列叠加,第1类与麻粒岩相变质发生于同 相似文献
118.
Dong Ok Lee 《The Professional geographer》1995,47(2):184-195
The locations of Korean firms in Los Angeles reflect spatial segmentation in markets and the provision of labor, in that the ethnic neighborhoods facilitate positive business environments for ethnic small firms. While Korean small businesses serve both community needs and broader local markets, firm locations are closely associated with the residential patterns of some ethnic populations. To address the specificity of firm locations, this paper examines Koreatown using historical sources and recent property development, identifies changes in the locational patterns of Korean firms between 1975 and 1986, and analyzes relationships between business density and density of ethnic populations for zip code areas. The findings of this study indicate that both the development of Koreatown and the presence of ethnic neighborhoods are important influences on the location of Korean businesses. 相似文献
119.
120.
林舸 《大地构造与成矿学》1990,14(4):305-313
本文综合分析了新疆北部重力场、磁场及地震等资料和邻区地质-地球物理资料,研究了新疆北部地质作用特征。依据岩石圈厚度,上地幔纵波速度和地壳结构等特征,将研究区分为阿尔泰、准噶尔、天山3个岩石圈块体,其间为深断裂带所分割;它们分别与不同发展期的地洼区(阿尔泰地洼区、准噶尔地洼区和天山地洼区)相对应。表明大地构造演化与深部地质作用过程密切相关。 相似文献