合肥盆地安参1井同位素年代地层学研究   总被引：4，自引：0，他引：4  

方小东  朱光  徐春华  刘国生 《安徽地质》2005,15(4):246-250

安参1井是胜利油田取得合肥盆地油气勘探权之后钻探的第一口区域探井,但因化石极少,更没有发现具有划时代意义的化石,因而选择K-Ar法、40^Ar／^39Ar法、砂岩中石英电子自旋共振（ESB）及大理岩的铅一铅等时线法测年等同位素年代学法进行安参1井的地层划分,从而准确地确定了安参1井的地层时代及划分界限,为合肥盆地基础地质的深入研究奠定了坚实的基础。  相似文献   

Genotoxicity in two bivalve species from a coastal lagoon in the south of Portugal     

《Marine environmental research》2013

DNA damage was evaluated by comet assay in the haemolymph of two bivalve species Ruditapes decussatus and Mytilus galloprovincialis from the Ria Formosa lagoon (south Coast of Portugal). Clams and mussels were sampled from sites close to each other to determine interspecific responses to similar environmental conditions, considering also seasonal and gender differences. Coupled with genotoxic effect, another damage biomarker (lipid peroxidation) was analysed to verify if the conditions that instigate DNA damage can be related with injury to cell membranes. For both species, DNA damage was low, reflecting the low levels of genotoxic contaminants in the lagoon, and no interspecific differences were found. However, seasonal differences were established for both bivalve species, reflecting higher environmental stress in summer. Regarding gender susceptibility, only clams showed differences in percentage of Tail DNA, with females more sensitive to DNA damage than males. Additionally, results for clams point out that factors responsible for LPO may not be the same as those causing genotoxicity. This study demonstrated that DNA damage is a sensitive biomarker to discriminate spatial, temporal and gender differences, being an appropriate biomarker for genotoxicity evaluation even in places of low contamination, such as the Ria Formosa lagoon.  相似文献   

热带北太平洋西部固氮速率及固氮微生物组成     

张润  张东声  陈敏  江志兵  王春生  郑敏芳  邱雨生  黄杰 《海洋学报(英文版)》2019,38(12):26-34

In the present study, we report N_2 fixation rate(~(15)N isotope tracer assay) and the diazotroph community structure(using the molecular method) in the western tropical North Pacific Ocean(WTNP)(13°–20°N, 120°–160°E). Our independent evidence on the basis of both in situ N_2 fixation activity and diazotroph community structure showed the dominance of unicellular N_2 fixation over majority of the WTNP surface waters during the sampling periods.Moreover, a shift in the diazotrophic composition from unicellular cyanobacteria group B-dominated to Trichodesmium spp.-dominated toward the western boundary current(Kuroshio) was also observed in 2013. We hypothesize that nutrient availability may have played a major role in regulating the biogeography of N_2 fixation.In surface waters, volumetric N_2 fixation rate(calculated by nitrogen) ranged between 0.6 and 2.6 nmol/(L·d) and averaged(1.2±0.5) nmol/(L·d), with 10 μm size fraction contributed predominantly(88%±6%) to the total rate between 135°E and 160°E. Depth-integrated N_2 fixation rate over the upper 200 m ranged between 150 μmol/(m~2·d)and 480 μmol/(m~2·d)(average(225±105) μmol/(m~2·d). N_2 fixation can account for 6.2%±3.7% of the depthintegrated primary production, suggesting that N_2 fixation is a significant N source sustaining new and export production in the WTNP. The role of N_2 fixation in biogeochemical cycling in this climate change-vulnerable region calls for further investigations.  相似文献   

地质样品中贵金属元素的预处理方法研究进展     

王烨  于亚辉  王琳  张明炜  黄杰  吴林海 《岩矿测试》2020,39(1):15-29

本文评述了近年来地质样品中贵金属元素分析预处理技术的研究现状和应用进展,对样品分解过程中常用的火试金法、碱熔融法、酸分解法以及样品分离富集过程中的吸附法、碲共沉淀法、离子交换法、溶剂萃取法、蒸馏法、生物吸附法等手段进行了归纳总结,分析了各方法的特点与不足,展望了技术方法未来发展方向。分解方法中的火试金法经分析工作者不断探索及改进,已成为分解贵金属的最佳手段,但其仍存在试剂消耗量大、成本高、流程长等缺点;碱熔融法虽可分解几乎所有地质样品,但其处理后的溶液存在大量钠盐,需经进一步的纯化;酸分解法主要以高压密闭和卡洛斯管的消解方式为主,但受到样品性质的制约。而不同分离富集的手段都具有较强的针对性,如:吸附法多用于Au、Pt、Pd的富集,蒸馏法仅适用于Os、Ru的分析。由于贵金属元素具有颗粒效应强、赋存形式复杂多样以及超痕量等特殊性,需要针对样品的类型特点选择相适应的预处理方法。本文提出,应当在现有的贵金属分解方法基础上,结合当前新的实验设备及实验条件,寻求更加高效、快捷的分解技术,严格控制流程的本底及各个环节的污染问题,实现多技术、多方法联用,满足贵金属分析的要求。  相似文献   

Occurrence of gonadtropins like substance in the thoracic ganglion mass of the mud crab, Scylla paramamosain(Crustacea: Decapoda: Brachyura)   总被引：1，自引：0，他引：1  

YEHaihui  HUANGHuiyang  WANGGuizhong  LIShaojing 《海洋学报(英文版)》2009,28(5):76-80

The identification and localization of vertebrate follicle stimulating hormone (FSH) and luteinizing hormone (LH) in crustaceans may further elucidate the regulation mechanisms in arthropod repro-duction.Using immunocytochemical staining techniques,this study has localized vertebrate FSH-like and LH-like substances in neurons in the subesophageal and thoracic ganglia from the thoracic ganglion mass (TGM) of Scylla paramamosain (Crustacea:Decapoda:Brachyura).Enzyme-linked immunosorbent assay (ELISA) has shown that the concentrations of both FSH-and LH-like sub-stances increased markedly in the TGM during the vitellogenic stage compared with that in the previtellogenic stage.These results indicate that substances resembling the vertebrate FSH and LH are present in S.paramamosain,and they may be involved in the development of the ovary as well as in ovulation.  相似文献   

海尔-波普彗星的球状喷出物     

钱伯辰  陶隽  顾敏峰  汤源 《中国天文和天体物理学报》1999,(1)

１９９６年９月观测到海尔－波普彗星的一次喷发,这次喷发持续了几天,在９月１０日和１１日分别观测到彗星的二个球状喷出物,并测得其投影喷出速度约为１００ｍ／ｓ,其后喷出物演变为巨大的喷流．  相似文献   

西藏拉果错卤虫──2.营养成分     

刘俊英  罗健  郑绵平 《湖泊科学》1999,11(3):283-288

卤虫包括成体、无节幼体和卤虫卵,蛋白质、氨基酸、脂肪酸等营养成分的含量是评价卤虫质量的主要标准,因为摄食动物饵料效果取决于氨基酸、脂肪酸的组成与含量.西藏拉果错卤虫的营养成分含量较高,1996、1997年盐湖研究中心连续取样测定,其蛋白质含量成体接近20%,无节幼体约 55%,脱壳卵近 50%;粗脂肪成体 3%,无节幼体和脱壳卵均为 15%左右;氨基酸总量成体 18%,无节幼体近 50%,脱壳卵 44%左右在对 1997年样品进行脂肪酸测定后发现,成体不饱和脂肪酸达83%,无节幼体91%以上,脱壳卵超过87%;三种高级不饱和脂肪酸(20∶2,20∶4,20∶5)HUFA含量:成体近8%,幼体和脱壳卵均超过20%;三种必需脂肪酸(18∶2,18∶3,20∶5)EFA含量:成体为16%,幼体21%,脱壳卵26%;无节幼体和脱壳卵的二十碳五烯酸(20∶5)EPA含量均在 18%以上,较我国新疆、青海、内蒙古、 山西、河北地区的盐湖卤虫和天津、海南盐田卤虫的(20:5)EPA含量高,脱壳卵的不饱和脂肪酸总量比已知的国内外其它盐湖卤虫的相应指数高  相似文献   

用双特异探针技术定性定量分析微型原甲藻   总被引：5，自引：0，他引：5  

蔡青松  李荣秀  甄毓  米铁柱  于志刚 《海洋学报》2006,28(6):127-133

针对微型原甲藻核糖体大亚基和小亚基RNA分别设计了大亚基探针(LSU probe)和小亚基探针(SSU probe),发展了对微型原甲藻进行定性和定量分析的双特异探针技术.分析数据表明针对微型原甲藻核糖体大亚基RNA的LSU probe能够特异地将微型原甲藻和其他7种微藻分开,而且检测信号的强弱在一定的范围内与细胞数呈线性关系;由于针对微型原甲藻核糖体小亚基的SSU probe探针由于与具齿原甲藻存在核酸序列一致性,该探针与具齿原甲藻有严重的交叉反应,但未发现与海洋原甲藻和其他藻的交叉反应.另外,研究还优化了破碎微型原甲藻细胞所需的超声时间以及获得探针的特异性所需的S1酶反应温度.本研究为实现对微型原甲藻海水样品的快速准确监测奠定了基础.  相似文献   

鼠尾藻中褐藻多酚化合物的抑菌活性研究   总被引：10，自引：0，他引：10  

林超  于曙光  郭道森  魏玉西  艾桂花 《海洋科学》2006,30(3):94-97

采用平板生长抑制法对鼠尾藻(SargassumthunbergiiKuntze)中多酚化合物的抑菌活性进行研究。结果表明,在一定浓度范围内试样对除大肠杆菌外的受试菌均有较明显的抑菌活性,抑菌活性的大小与多酚浓度和分子质量密切相关。其中,透析内液对溶藻胶弧菌(Vibrioalginolyticus)和鳗弧菌(V.anguillarum)、透析外液对鳗弧菌和副溶血弧菌(V.parahaemolyticus)的抑制效果尤其明显。  相似文献   

瓦氏黄颡鱼(Pelteobagrus vachelli)卵黄蛋白原的纯化、性质鉴定及ELISA检测方法的建立   总被引：1，自引：0，他引：1  

李育培  刁晓明  盛晓洒  权恒  翟旭亮  李云 《海洋与湖沼》2010,41(1):91-98

腹腔注射17β-雌二醇(E2),使瓦氏黄颡鱼雄鱼在7天内产生卵黄蛋白原(Vtg)。采用凝胶过滤和离子交换两种层析技术,从E2诱导的雄性瓦氏黄颡鱼血浆中分离、纯化出Vtg,采用糖、磷、脂蛋白染色技术证明分离、纯化的蛋白为Vtg,该Vtg在非变性条件下分子量约为240kDa,在SDS变性条件下分子量约为143kDa。纯化的瓦氏黄颡鱼Vtg经检测显示可能含有类胡萝卜素,但没有二硫键,对热相对稳定。利用纯化的瓦氏黄颡鱼Vtg,制备了兔抗瓦氏黄颡鱼Vtg多克隆抗血清。用双向免疫扩散法测得抗血清的纯度较高,效价为1︰32;Western blotting检测显示抗血清的特异性较好。以瓦氏黄颡鱼Vtg多克隆抗血清为抗体,以纯化的瓦氏黄颡鱼Vtg为抗原,建立了间接酶联免疫吸附反应(ELISA)方法检测瓦氏黄颡鱼体内Vtg的含量,标准曲线线性部分的线性方程为y=0.099x+0.4529(R2=0.9327),该方法检测的灵敏度为15.6ng/ml,工作范围为31.2—4000ng/ml,在此范围内,标准曲线具有良好的线性。  相似文献