Analysis of bacterial diversity and metals in produced water, seawater and sediments from an offshore oil and gas production platform     

Yeung CW  Law BA  Milligan TG  Lee K  Whyte LG  Greer CW 《Marine pollution bulletin》2011,62(10):2095-2105

Produced water is one of the largest waste products routinely discharged into the ocean from offshore oil and gas platforms. This study analyzed bacterial communities and metals in the produced water, surrounding seawater, and sediment around the Thebaud platform. The bacterial community within the produced water was different from the seawater (SAB = 13.3), but the discharge had no detectable effect on the bacterial communities in the seawater (SAB > 97). In contrast, genomic analysis of sediments revealed that the bacterial community from 250 m was different (SAB = 70) from other locations further from the discharge, suggesting that the produced water had a detectable effect on the bacterial community in the sediment closest to the discharge. These near-field sediments contained elevated concentrations of manganese and iron that are associated with the produced water effluent. The results suggested that the discharge of produced water has influenced the bacterial community structure of sediments adjacent to the platform.  相似文献   

Effects of contamination of single and combined cadmium and mercury on the soil microbial community structural diversity and functional diversity     

Xiaomei Xie  Min Liao  Aili Ma  Haijun Zhang 《中国地球化学学报》2011,30(3):366-374

To assess the effects of single and combined pollution of cadmium (Cd) and mercury (Hg) on soil micro-bial community structural and functional diversities, an incubation experiment was conducted, by employing two soils, namely, the marine sediment silty loam soil and the yellowish-red soil, in which five levels of Cd, Hg and Cd and Hg in combination were added. After being incubated for 56 days, the phospholipid fatty acids (PLFAs) profile and sole carbon source utilization pattern (BIOLOG) of the samples were tested. The results showed that the compo-sition of the microbial communities changed significantly at different levels of metals application. The principal component analyses (PCA) of PLFAs indicated that the structure of the microbial community was also significantly altered with increasing levels of metals, with increasing PLFAs biomarkers for fungi and actinomycetes, and in-creasing ratio of Gram-positive to Gram-negative bacteria. Sole carbon source utilization pattern analysis revealed that single and combined application of Cd and Hg inhibited significantly the functional activity of soil microorgan-isms, the functional diversity indices [Richness (S), Shannon-Wiener indices (H) and Evenness (EH)] were signifi-cantly lower in polluted soils than those in non-polluted soils, which also significantly altered with increasing levels of metals. PCA for the sole carbon source utilization pattern also indicated that the metal contamination could result in a variable soil microbial community. The results revealed that the combination of Cd and Hg had higher toxicity to soil microbial community structural and functional diversities than the individual application of Cd or Hg.  相似文献   

日本蟳(Charybdis japonica)微卫星位点的分离及遗传多样性分析          下载免费PDF全文

叶茂  申望  王日昕  郑兆祥  石戈 《海洋与湖沼》2011,42(1):86-93

采用磁珠富集法,以生物素标记的(cA)12寡核苷酸为探针,构建了日本蟳基因组微卫星富集文库.通过PCR法从文库中共筛选出89个微卫星位点,其中完全型(perfect)共75个,占84.27%;不完全型(imperfect)12个,占13.48%;复合型(compound)2个,占2.25%.根据微卫星位点的侧翼序列,设...  相似文献   

2003年夏季西北冰洋浮游动物群落结构和地理分布研究   总被引：1，自引：1，他引：0       下载免费PDF全文

张光涛  孙松 《海洋学报》2011,33(2):146-156

根据西北冰洋43个调查站位的浮游动物种类组成和数量资料,分析了浮游动物的群落结构和地理分布特征,探讨了浮游动物群落与环境因子的关系.结果表明,在调查区域存在三种不同的浮游动物群落类型:楚科奇海台和加拿大海盆地区的高纬度深海群落;楚科奇海中部的陆架群落;阿拉斯加沿岸和楚科奇海北部的沿岸过渡群落.深海群落浮游动物数量较少,...  相似文献   

闽粤群和岱衢群养殖大黄鱼（Pseudosciaenacrocea）及其杂交子代遗传差异的SSR分析   总被引：1，自引：0，他引：1  

黄振远  苏永全  张建设  吴常文 《海洋与湖沼》2011,42(4):592-596

采用SSR方法进行了闽粤群（MY）和岱衢群（DQ）及其正交和反交大黄鱼的遗传差异的研究。结果表明,Shannon’S多样性指数为DQ群高于MY群,分别为2．14、2．04;反交群高于正交群分别为2．13、2．03：杂交群体中为2．24与其亲体群的相似（2．22）。亲体群间基因分化系数只t值为0．020,低于杂交后代的0...  相似文献   

半滑舌鳎（Cynoglossus semilaevis）野生与养殖群体遗传多样性的比较研究          下载免费PDF全文

黎中宝  雷光高  常建波  赵斌丽  陈锦  张桂玲  王展林 《海洋与湖沼》2011,42(3):414-418

采用扩增片段长度多态性技术（AFLP技术）对2个野生和2个养殖的半滑舌鳎群体进行了遗传多样性的比较研究。实验采用8对AFLP引物组合,在四个群体的120个个体中共扩增出498个位点。结果表明,半滑舌鳎养殖群体的遗传多样性降低：河北唐山野生群体和江苏连云港野生群体的多态位点百分数分别为45．18％和39．96％,Nei遗...  相似文献   

长蛸（Octopus variabilis）不同地理群体遗传多样性的ISSR分析     

郭宝英  周超  吕振明  李继姬  吴常文 《海洋与湖沼》2011,42(6):868-873

利用ISSR分子标记技术获得长蛸的ISSR-PCR最优反应体系,并对大连、烟台、青岛、连云港、舟山、温州、厦门7个群体168个个体进行遗传多样性分析。结果表明,7个引物在7个群体中共扩增出118条重复性好且带型清晰的ISSR扩增带,其中多态条带为55条,多态性比例达46.61%。在物种水平上的Nei’s基因多样性指数和...  相似文献   

Benthic habitat mapping: Concerns using a combined approach (acoustic, sediment and biological data)     

Rosa Freitas  Fernando Ricardo  Fbio Pereira  Leandro Sampaio  Susana Carvalho  Miguel Gaspar  Victor Quintino  Ana Maria Rodrigues 《Estuarine, Coastal and Shelf Science》2011,92(4):72

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Coastal Mediterranean plankton stimulation dynamics through a dust storm event: An experimental simulation     

Estela Romero  Francesc Peters  Cèlia Marrasé  Òscar Guadayol  Josep M. Gasol  Markus G. Weinbauer 《Estuarine, Coastal and Shelf Science》2011

An enhancement of aeolian inputs to the ocean due to a future increase in aridity in certain parts of the world is predicted from global change. We conducted an experimental simulation to assess the biological response of NW Mediterranean coastal surface waters to an episodic dust addition. On the assumption that planktonic growth was limited by phosphorus, dust effects were compared to those induced by equivalent enrichments of phosphate. The experiment analyzed the dynamics of several parameters during one week: inorganic nutrients, total and fractioned chlorophyll a, bacterial abundance, phytoplankton species composition, abundance of autotrophic and heterotrophic flagellates, particulate organic carbon and particulate organic nitrogen. The maximum addition of dust (0.5 g dust L⁻¹) initiated an increase in bacterial abundance. After 48 h, bacterial numbers decreased due to a peak in heterotrophic flagellates and a significant growth of autotrophic organisms, mainly nanoflagellates but also diatoms, was observed. Conversely, lower inputs of dust (0.05 g dust L⁻¹) and phosphate enrichments (0.5 μmol PO₄³⁻ L⁻¹) only produced increases in phototrophic nanoflagellates. In our experiment, dust triggered bacterial growth, changed phytoplankton dynamics and affected the ratio of autotrophic to heterotrophic biomass, adding to the variability in the sources that affect system dynamics, energy and carbon budgets and ultimately higher trophic levels of the coastal marine food web.  相似文献   

Seagrass colonization: Knock-on effects on zoobenthic community,populations and individual health     

V. Tu Do  Xavier de MontaudouinNicolas Lavesque  Hugues BlanchetHervé Guyard 《Estuarine, Coastal and Shelf Science》2011

This study provided evidence that Zostera noltii presence affects macrofauna community structure independently from median sediment grain-size and that the notion of ecosystem health is rather subjective: in the present case, we recorded “good health” in terms of seagrass development, “no impact” in terms of macrobenthic biotic indices and “negative effect” for a given key-population. The occurrence and development of a Z. noltii seagrass bed was surveyed at Banc d’Arguin, Arcachon Bay (France), to estimate the modification of the macrozoobenthic community and of the dynamics of a key-population for the local ecosystem, – the cockle Cerastoderma edule. Even though median grain-size of the sediment decreased only at the very end of the survey, i.e. when seagrass totally invaded the area, most of the macrofauna community characteristics (such as abundance and biomass) increased as soon as Z. noltii patches appeared. The structure of the macrofauna community also immediately diverged between sand and seagrass habitats, without however modifying the tested biotic indices (BENTIX, BOPA, AMBI). The health of the cockle population (growth, abundance, recruitment) was impacted by seagrass development. Related parasite communities slowly diverged between habitats, with more parasites in the cockles from seagrass areas. However, the number of parasites per cockle was always insufficient to alter cockle fitness.  相似文献