凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析   总被引：16，自引：0，他引：16  

罗鹏  胡超群  谢珍玉  张吕平  任春华  许尤厚 《热带海洋学报》2006,25(2):49-53

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。  相似文献   

Colonization, succession, and nutrition of macrobenthic assemblages in a restored wetland at Tijuana Estuary, California     

Serena M. Moseman  Lisa A. Levin  Carolyn Currin  Charlotte Forder   《Estuarine, Coastal and Shelf Science》2004,60(4):755-770

Modes of colonization, the successional trajectory, and trophic recovery of a macrofaunal community were analyzed over 19 months in the Friendship marsh, a 20-acre restored wetland in Tijuana Estuary, California. Traditional techniques for quantifying macrofaunal communities were combined with emerging stable isotopic approaches for evaluation of trophic recovery, making comparisons with a nearby natural Spartina foliosa habitat. Life history-based predictions successfully identified major colonization modes, although most taxa employed a variety of tactics for colonizing the restored marsh. The presence of S. foliosa did not seem to affect macrofaunal colonization or succession at the scale of this study. However, soil organic matter content in the restored marsh was positively correlated with insect densities, and high initial salinities may have limited the success of early colonists. Total macrofaunal densities recovered to natural marsh levels after 14 months and diversity, measured as species richness and the Shannon index (H′), was comparable to the natural marsh by 19 months. Some compositional disparities between the natural and created communities persisted after 19 months, including lower percentages of surface-feeding polychaetes (Polydora spp.) and higher percentages of dipteran insects and turbellarians in the Friendship marsh. As surficial structural similarity of infaunal communities between the Friendship and natural habitat was achieved, isotopic analyses revealed a simultaneous trajectory towards recovery of trophic structure. Enriched δ¹³C signatures of benthic microalgae and infauna, observed in the restored marsh shortly after establishment compared to natural Spartina habitat, recovered after 19 months. However, the depletion in δ¹⁵N signatures of macrofauna in the Friendship marsh indicated consumption of microalgae, particularly nitrogen-fixing cyanobacteria, while macroalgae and Spartina made a larger contribution to macrofaunal diets in the natural habitat. Future successional studies must continue to develop and employ novel combinations of techniques for evaluating structural and functional recovery of disturbed and created habitats.  相似文献   

Modelling phytoplankton succession on the Bering Sea shelf: role of climate influences and trophic interactions in generating Emiliania huxleyi blooms 1997–2000     

Agostino Merico  Toby Tyrrell  Evelyn J. Lessard  Temel Oguz  Phyllis J. Stabeno  Stephan I. Zeeman  Terry E. Whitledge 《Deep Sea Research Part I: Oceanographic Research Papers》2004,51(12):1929

Several years of continuous physical and biological anomalies have been affecting the Bering Sea shelf ecosystem starting from 1997. Such anomalies reached their peak in a striking visual phenomenon: the first appearance in the area of bright waters caused by massive blooms of the coccolithophore Emiliania huxleyi (E. huxleyi). This study is intended to provide an insight into the mechanisms of phytoplankton succession in the south-eastern part of the shelf during such years and addresses the causes of E. huxleyi success by means of a 2-layer ecosystem model, field data and satellite-derived information. A number of potential hypotheses are delineated based on observations conducted in the area and on previous knowledge of E. huxleyi general ecology. Some of these hypotheses are then considered as causative factors and explored with the model. The unusual climatic conditions of 1997 resulted most notably in a particularly shallow mixed layer depth and high sea surface temperature (about 4 °C above climatological mean). Despite the fact that the model could not reproduce for E. huxleyi a clear non-bloom to bloom transition (pre- vs. post-1997), several tests suggest that this species was favoured by the shallow mixed layer depth in conjunction with a lack of photoinhibition. A top-down control by microzooplankton selectively grazing phytoplankton other than E. huxleyi appears to be responsible for the long persistence of the blooms. Interestingly, observations reveal that the high N:P ratio hypothesis, regarded as crucial in the formation of blooms of this species in previous studies, does not hold on the Bering Sea shelf.  相似文献   

Organic enrichment and structure of the macrozoobenthic community in the northern Adriatic Sea in an area facing Adige and Po mouths   总被引：1，自引：0，他引：1  

Simonini  R.; Ansaloni  I.; Bonvicini Pagliai  A.M.; Prevedelli  D. 《ICES Journal of Marine Science》2004,61(6):871-881

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Variation in microbial biomass and community structure in sediments of peter the great bay (sea of japan/east sea), as estimated from fatty acid biomarkers     

Natalia V. Zhukova 《Ocean Science Journal》2005,40(3):34-42

Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria -were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.  相似文献   

Vertical distribution of the macrobenthic community and its relationships to trace metals and natural sediment characteristics in the lower Douro estuary, Portugal   总被引：2，自引：0，他引：2  

Ana P. Mucha  M. Teresa S. D. Vasconcelos  Adriano A. Bordalo   《Estuarine, Coastal and Shelf Science》2004,59(4):663

Vertical distribution (0–15 cm) of the macrobenthic community and its relationships to natural sediment characteristics and trace metal contents and bioavailability were studied at five locations in the lower Douro estuary, Portugal. An analysis of vertical metal distribution, for the interpretation of anthropogenic impact on the estuarine sediments, was also investigated. Sediment characterisation included organic matter, grain size, metals (Al, Fe, Cu, Pb, Cr, Ni, Cd, Zn and Mn), acid volatile sulphide (AVS) and simultaneously extracted metals (SEM). The macrobenthic community had low diversity (14 species), was dominated by small size opportunists and seemed to be controlled mainly by natural factors such as grain size distribution, Al and Fe contents and sediment depth. The vertically heterogeneous distribution of macrobenthic community appears to affect redox status of the sediments and consequently metal bioavailability. Despite anthropogenic contamination in terms of Zn, Cu, Pb, Cr and Ni having already been detected in the north bank, the analysis of vertical distribution was essential for the identification of current anthropogenic contamination in terms of Zn, Pb and Cd in the south bank.  相似文献   

Mussel Periostracum from Deep-Sea Redox Communities as a Microbial Habitat: 3. Secondary Inhabitants     

James E.  Hook Stjepko  Golubic 《Marine Ecology》1992,13(2):119-131

Abstract. Vacated microborings in periostracum of live mussels of the Florida Escarpment redox community (depth of 3266 m) become a habitat for a prolific secondary microbiota consisting of a variety of prokaryotic and a few eukaryotic organisms. Periostracum surface is also colonized by diverse microorganisms, dominated by presumed chemolithotrophic bacteria with stacks of intracellular lamellae. Unlike sheltered microflora within borings, the surface community is heavily grazed upon by numerous archaeogastropods and ciliates.  相似文献   

Effects of a Short-term Environmental Change on a Brackish-water Polychaete Community     

ADRIANA GIANGRANDE  SIMONEITA FRASCHETTI 《Marine Ecology》1996,17(1-3):321-332

Abstract. Aquatina lake is a brackish basin, connected with the Adriatic Sea along the mast of Apulia (Italy), with a mean salinity of 26%. The abundance and biomass of fifteen polychaete species were recorded by monthly samples in a pilot area of the lake from February 1989 to February 1990. Naineris laevigata was dominant, both in number of individuals and biomass. During 1990, obstruction of freshwater inflow to the lake caused an increase in salinity up to 4O%, followed by some changes in the polychaete community. Noromasrus hiericeus became dominant, and the abundances of the other species decreased except for Naineris laevipru . Salinity assumed normal values after three months, but community responded slowly becauie the massive presence of N. latericeus inhibited the recovery of other species. An abiotic disturbance, followed by a biotic disturbance, altered the structure of the polychaete community.  相似文献   

Community Structure and Dynamics of Phytoplankton in the Western Subarctic Pacific Ocean: A Synthesis   总被引：1，自引：1，他引：1  

Hongbin Liu  Koji Suzuki  Hiroaki Saito 《Journal of Oceanography》2004,60(1):119-137

The phytoplankton community in the western subarctic Pacific (WSP) is composed mostly of pico- and nanophytoplankton. Chlorophyll a (Chl a) in the <2 μm size fraction accounted for more than half of the total Chl a in all seasons, with higher contributions of up to 75% of the total Chl a in summer and fall. The exception is the western boundary along the Kamchatka Peninsula and Kuril Islands and the Oyashio region where diatoms make up the majority of total Chl a during the spring bloom. Among the picophytoplankton, picoeukaryotes and Synechococcus are approximately equally abundant, but the former is more important in term of carbon biomass. Despite the lack of a clear seasonal variation in Chl a concentration, primary productivity showed a large seasonal variation, and was lowest in winter and highest in spring. Seasonal succession in the phytoplankton community is also evident with the abundance of diatoms peaking in May, followed by picoeukaryotes and Synechococcus in summer. The growth of phytoplankton (especially >10 μm cell size) in the western subarctic Pacific is often limited by iron bioavailability, and microzooplankton grazing keeps the standing stock of pico- and nano-phytoplankton low. Compared to the other HNLC regions (the eastern equatorial Pacific, the Southern Ocean, and the eastern subarctic Pacific), iron limitation in the Western Subarctic Gyre (WSG) may be less severe probably due to higher iron concentrations. The Oyashio region has similar physical condition, macronutrient supply and phytoplankton species compositions to the WSG, but much higher phytoplankton biomass and primary productivity. The difference between the Oyashio region and the WSG is also believed to be the results of difference in iron bioavailability in both regions. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Evaluating fishery impacts using metrics of community structure   总被引：8，自引：14，他引：8  

Rice  Jake C. 《ICES Journal of Marine Science》2000,57(3):682-688

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