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鱼类生长激素的异源表达、应用及安全性评价 总被引:3,自引:0,他引:3
鱼类生长激素对鱼的生长发育起着重要的调节作用,能促进鱼体快速生长,提高饵料转化率,在水产养殖业中具有重大应用价值。转生长激素基因鱼具有快速生长效应,可以缩短养殖周期,降低养殖成本和风险。鱼类生长激素基因异源表达体系的建立和发展,使获得大量廉价的鱼类生长激素产品成为可能,为鱼类养殖业新型饵料添加剂的研制开辟了新的途径。本文综述了转生长激素基因鱼和鱼类生长激素基因的异源表达两方面的研究及其安全性评价。 相似文献
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本实验采用雄性褐菖鲉作为实验对象,腹腔注射0.5×10-6、1×10-6、5×10-6、10×10-6(m/m)苯并[a]芘,7d后苯并[a]芘处理组精巢睾酮水平和睾酮与17β-雌二醇的比值都有上升,但都未表现出显著性差异(P>0.05).腹腔注射50×10-6(m/m)苯并[a]芘,11d后取出精巢,常规石蜡切片,结果显示雄性褐菖鲉的精巢,精小叶腔和输出管中精子密集程度明显增加,次级精母细胞减少,精巢壁厚度变薄.结果提示,苯并[a]芘对褐菖鲉具有类雄激素效应. 相似文献
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Yubin JI Chenfeng JI Xiang ZOU Huixin LIU 《中国地球化学学报》2006,25(B08):186-186
To detect the excretion ratio of Toluene Diisocyanate (TDI) in mice feces by HPLC. The chromatogram column is C18-ODS, the mobile phase is Acetonitrile -H2O (45 : 55), the flow rate is 1mL/min. The detection wavelength is 254 nm, and the quantity is determined by outer mark method. The linear relationship of concentrations of TDI was shown well in the range of 1.2 mol/L-5.2 mol/L, and the recovery ratio was 96.48%, precision degree within the day was under 2.9% and in the day it was under 3.37%. This method was simple, convenient and viable. And the result showed that the excretive ratio of TDI in mice feces was distinctly increased with the dosage given by p.o, which proved that feces were the chief mode of archetypal excretion of TDI given by p.o. 相似文献
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p-nitrotoluene is an important organic intermediate widely used in pesticide, foamed plastics, dyestuff and medicine industries.
In this paper, Crusian carps (Carassius auratus) were exposed to dilute p-nitrotoluene solutions with different concentrations of 0, 20, 40, 80, 120, 160, 240 and 320 μg/L; the subsequent physiological
responses to the chemical were observed, and the variation characteristics of blood corpuscles, ovaries and oocytes were studied
by bio-microscopy. Results showed that even very low concentrations of p-nitrotoluene would lead to toxicosis and even the death of Carassius auratus. The bio-microscopic structures revealed that, compared with the compartment of Carassius auratus growing in the p-nitrotoluene-free water (the control group), the lower concentrations of p-nitrotoluene (for example no more than 80 μg/L) would result in the faster disintegration of erythrocytes and the significant
enlargement of oocytes, while the higher contents of p-nitrotoluene (no less than 240 μg/L in this case) would inhibit the division of cells and their further growth, even degenerate
blood corpuscles and ovaries, suggesting stronger estrogenic activities.
The study is financially supported by the Development Foundation of Science and Technology under Shandong Provincial Education
Department (No. J05D51). 相似文献
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本实验旨在探究维生素E对半滑舌鳎垂体组织中生长激素基因表达的影响。作者在每千克等氮等能的基础饲料中分别添加0、400和1 600mg的DL-α-生育酚乙酸酯(维生素E)投喂半滑舌鳎(Cynoglossus semilaevis)成鱼(464±2.6) g,进行为期8周的养殖实验;另外,在L-15培养基中添加0、18和54μmol/L的维生素E,对半滑舌鳎成鱼(464±2.6) g的垂体细胞进行为期3 d的体外原代培养实验。分别取垂体组织和原代细胞,通过荧光实时定量PCR分析其gh mRNA的相对表达量。实验结果表明:垂体组织中gh mRNA的相对表达量随着饲料中维生素E含量的增加而呈现先升高后下降的变化趋势,在400 mg/kg组时显著高于其他各组(P0.05);随着细胞培养液中维生素E浓度的升高, gh mRNA的相对表达量显著增加(P0.05)。由此可见,适宜浓度的维生素E能够促进半滑舌鳎垂体组织中生长激素基因的表达。 相似文献
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对产自河北围场下白垩统九佛堂组的65件鲎虫化石标本进行研究。根据其形态特征,认为这些鲎虫化石属于三角围场鲎虫(Weichangiops triangularisYang et Hong, 1980)。有观点认为,具有上肛板的鲎虫为冷水型鲎虫,而不具上肛板的鲎虫为暖水型鲎虫。河北围场采集的鲎虫化石有的具上肛板,有的不具上肛板,目前没有任何证据表明其为冷水型鲎虫与暖水型鲎虫混生的产物。通过研究,对三角围场鲎虫的特征进行了适当修正,认为围场地区的鲎虫化石具1对复眼,因此依据单个复眼而建立的新亚科——围场鲎虫亚科(Weichangiopsinae)不成立,应予以摒弃。三角围场鲎虫存在明显的性双形现象,雄性个体尾部具有上肛板,雌性个体不具有上肛板,这对于鲎虫化石分类研究具有重要的科学价值,可避免在生物分类时发生同物异名现象。 相似文献
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通过腹腔注射药物、尾静脉取血、放射免疫测定激素含量等方法研究L-多巴等几种药物对草鱼鱼种生长激素分泌的影响,发现腹腔注射L-多巴、阿朴吗啡可以显著提高草鱼鱼种血清生长激素水平,而腹腔注射多巴胺、去甲肾上腺素、肾上腺素和6-羟多巴则可明显降低草鱼鱼种血清生长激素水平。说明儿茶酚胺类参与了草鱼鱼种生长激素的分泌调节,L-多巴或其代谢物是通过中枢神经系统促进草鱼鱼种生长激素分泌的,而多巴胺、去甲肾上腺素和肾上腺素则是在脑垂体水平上抑制草鱼鱼种生长激素的分泌。 相似文献
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Feldspar phenocrysts of silicic volcanic rocks are commonly in Sr-isotopic disequilibrium with groundmass. In some cases the feldspar is more radiogenic, and in others it is less radiogenic. Several explanations have been published previously, but none of these is able to accommodate both senses of disequilibrium. We present a model by which either more- or less-radiogenic feldspar (or even both within a single eruptive unit) can originate. The model requires a magma body open to interaction with biotite- and feldspar-bearing wall rock. Magma is incrementally contaminated as wall rock melts incongruently. Biotite preferentially melts first, followed by feldspar. Such melting behavior, which is supported by both field and experimental studies, first contaminates magma with a relatively radiogenic addition, followed by a less-radiogenic addition. Feldspar phenocrysts lag behind melt (groundmass of volcanic rock) in incorporating the influx of contaminant, thus resulting in Sr-isotopic disequilibrium between the crystals and melt. The sense of disequilibrium recorded in a volcanic rock depends on when eruption quenches the contamination process. This model is testable by isotopic fingerprinting of individual feldspar crystals. For a given set of geologic boundary conditions, specific core-to-rim Sr-isotopic profiles are expectable. Moreover, phenocrysts that nucleate at different times during the contamination process should record different and predictable parts of the history. Initial results of Sr-isotopic fingerprinting of sanidine phenocrysts from the Taylor Creek Rhyolite are consistent with the model. More tests of the model are desirable. 相似文献