褐点石斑鱼不同组织4种同工酶的研究   总被引：5，自引：0，他引：5  

尹绍武  廖经球  黄海  雷从改  陈国华  张本 《海洋通报》2007,26(1):41-44

运用聚丙烯酰胺垂直梯度凝胶电泳法对褐点石斑鱼7种组织(眼睛、肌肉、心脏、肝脏、肾脏、尾鳍、脾脏)的4种同工酶(EST、SOD、LDH、MDH)进行了初步研究。结果表明:褐点石斑鱼的4种同工酶系统具有不同程度的组织特异性。EST由4个基因位点编码,具有多态性现象;肌肉SOD与其它组织SOD有明显差异;眼睛LDH酶谱酶带最多;MDH具有线粒体型(m-MDH)和上清液型(s-MDH)两种类型。  相似文献   

ALLOZYME VARIATION IN SCAPHARCA SUBCRENATA FROM QINGDAO COASTAL WATERS     

喻子牛  孔晓瑜  杨锐  王如才  刘必谦 《中国海洋大学学报(自然科学版)》1997,(4)

采用淀粉凝胶电泳技术研究了青岛近海毛蚶自然群体的等位基因酶遗传变异。在十二种等位基因酶中共检测到了二十七个基因座位，多态位点比例为５１．８５％，位点有效等位基因数分布从１．０３０…２．５７７（平均值１．３８１±０．３４５），位点杂合度观察值分布为０．０２９…０．６１２（平均杂合度０．１３３±０．０３６），在六个基因座位上存在相当明显的杂合子缺失现象。文中讨论了杂合子缺失的原因。  相似文献   

Comparison of growth rate among different protein genotypes in Atlantic cod, Gadus morhua, under farmed conditions     

Jorstad  K.E.; Karlsen  O.; Svasand  T.; Ottera  H. 《ICES Journal of Marine Science》2006,63(2):235-245

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GENETIC CONSEQUENCES OF INTRODUCED Gouramy Helostoma Temminckii (Pisces: Helostomatidae) IN BRUNEI‘S WETLAND     

H.S.Zohrah　 P.H.C.Fauzani　 C. S. Jen　O. Norsuzieyanti 《湿地科学》2003,1(1):51-56

Kissing gouramy ( Helostoma temminckii) was introduced into Tasek Merimbun in the 1980 s. We studied their genetic profile using allozyme electrophoresis and found that all loci were monomorphic. The loss of het-erozygotes may suggest that the gouramy are inbreds. They must have reproduced by repeated sib-mating over the years. The severe drought in Tasek Merimbun may have aggravated the genetic erosion further. The genetic variability of kissing gouramy in terms of % polymorphic loci, mean number of alleles per loci and observed heterozygosity level were lower than Cyprinids in the same lake and kissing gouramy from the wild. We also note that kissing gouramy is a fast grower and may threaten the livelihood of indigenous fishes in the lake.  相似文献   

Comparative Binding of Endocrine Disrupting Compounds and Pharmaceuticals with Polydopamine‐ and Polypyrrole‐coated Magnetic Nanoparticles          下载免费PDF全文

Musharraf Miah  Zafar Iqbal  Edward P.C. Lai 《洁净——土壤、空气、水》2015,43(2):173-181

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Peroxisomal proteomics: Biomonitoring in mussels after the Prestige’s oil spill     

Itxaso Apraiz  Miren P. Cajaraville  Susana Cristobal 《Marine pollution bulletin》2009,(12):1815-1826

Peroxisomal proteomics was applied to assess possible biological effects after the Prestige’s oil spill. Mussels were sampled in July 2004 and 2005 in four stations in the NW (closest to the spill) and NE coasts of the Iberian Peninsula. Principal components analysis (PCA) suggested differences in protein expression among stations and sampling years. Several proteins were putatively identified by mass spectrometry and immunolocalization. PC1 separated the NW stations in 2004 from the rest of the stations and sampling years mainly due to up-regulation of peroxisomal β-oxidation proteins and PMP70. PC3 separated the NE stations, based on up-regulation of the antioxidant enzyme catalase in 2004 compared to 2005. PC4 separated the stations in the NE and the NW. This work shows that environmental proteomics, together with multivariate data analysis, could provide information to interpret the effects of oil spills at cellular level in mussels.  相似文献   

污水人工快速渗滤系统中氨氧化菌16S rDNA的DGGE分析     

马鸣超  姜昕  刘菲  李俊 《高校地质学报》2007,13(4):688-693

为了研究污水人工快速渗滤系统(CRI)中氨氧化菌的空间分布规律以及NH4-N在CRI中的迁移转化机理,从深圳运行中的CRI系统砂层填料中以5~10cm间隔垂直取12个样品,对其氨氧化菌的16SrDNA进行DGGE分析。结果表明,CRI系统中氨氧化菌菌群随着深度的增加先增多后减少,在表层至10cm处氨氧化菌约有4~5种组成,主要由某些以有机氮为降解底物的微生物和进水中被砂粒介质截流吸附的微生物组成;在20~90cm范围内氨氧化菌增加至9种左右,多样性最为丰富,是亚硝化作用发生的主要区域;而在100cm以下的范围,由于NH4-N浓度的降低或系统复氧不足,氨氧化菌迅速减少至5~6种。该研究结果证明了氨氧化菌在快渗池内分布范围的变化对NH4-N去除率的影响,即氨氧化菌在快渗池内分布的越接近中上层,对NH4-N的去除效果越好。关于CRI系统中氨氧化菌的空间分布规律及其与NH4-N去除率关系的认识,为工艺改进和效果提高提供了理论基础。  相似文献   

Biochemical, chromatographic and spectroscopic analyses of Suwannee River fulvic acid-Evidence for the presence of a DNA-like material     

Xiaodong MA Nawab ALI 《中国地球化学学报》2006,25(B08):267-267

Naturally occurring organic compounds are complicated materials that are hard to fractionate into individual components. Organic materials from lakes, rivers and oceans often contain biological molecules related to proteins and DNA, and have global environmental and ecological importance. In this study, we used a combination of biochemical, chromatographic and spectroscopic methods to fractionate and characterize Suwannee River fulvic acid （SRFA） to determine if it contains proteins and DNA or DNA-like materials. A stock solution of SRFA （50 mg/mL） was prepared in double distilled deionized water. An initial biochemical analysis indicated that SRFA contained approximately 2% protein by weight, as determined by Bradford method using bovine serum albumin as standard. In order to determine the presence of DNA-like materials, a portion of the SRFA sample was extracted for DNA using chloroform/phenol extraction and ethanol precipitation method. When ethanol precipitated fraction was separated by agarose gel electrophoresis along with known DNA markers, a diffused ehthidium bromide staining was observed throughout the lane containing SRFA sample with a strong staining observed at the dye front, suggesting the presence of DNA or ＂DNA-like＂ molecules of various sizes in SRFA. To further fractionate, the DNA-like material on the gel was cut into seven fractions, each of which was extracted into aqueous phase. These fractions, along with the fractions obtained similarly from the original SRFA and an interface material during chloroform/ phenol extraction, were analyzed using UV-vis and fluorescence spectrometers, and LC/UV/MS. Comparisons of the absorption, fluorescence and mass spectra of various fractions revealed significant differences in their spectral properties. The DNA extracted materials showed stronger absorption in the DNA range of -260 nm than the original SRFA and interface material.  相似文献   

虾夷扇贝性腺和闭壳肌蛋白质双向电泳分析     

沈妍  刘伟  湛垚垚  黄显雅  穆琳琳  徐蕾  丁君  常亚青 《海洋科学》2014,38(6):10-16

本研究建立了虾夷扇贝性腺、闭壳肌蛋白质双向电泳体系,比较分析了不同等电聚焦时间对虾夷扇贝性腺、闭壳肌蛋白质双向电泳结果的影响,进一步利用该双向电泳系统分离了性腺和闭壳肌可溶性全蛋白,并利用计算机图像分析软件对虾夷扇贝性腺和闭壳肌蛋白质双向电泳图谱进行了检测分析。实验结果表明,采用细胞超声破碎-丙酮沉淀除盐–50 mmol/L Tris-HCl(pH6.8)复溶,1000V,3 h等电聚焦,12%SDS-PAGE和高灵敏度银氨染色,可获得分辨率较高、重复性良好的双向电泳图谱。通过PDQuest8.0图像分析软件检测分析,发现pH5~8条件下,虾夷扇贝性腺和闭壳肌组织的蛋白质主要分布于pH5~7,相对分子质量97~20.1 ku范围,性腺组织双向电泳图谱检出的蛋白质斑点数为265个,闭壳肌组织检出161个蛋白质斑点。结果显示,虾夷扇贝性腺、闭壳肌可溶性蛋白质表达谱各具特点,性腺组织可溶性蛋白种类较闭壳肌组织丰富。本研究实验结果可为进一步研究扇贝蛋白质组学以及功能基因组学奠定一定的基础。  相似文献   

Role of asymmetry effect on the specific conductivity of water—A case study     

Parameswara D.  Prasad K.S.S. 《中国地球化学学报》2012,31(3):276-281

Forty-one water samples in each season(both pre-and post-monsoon) were collected from different for-mations of the Kadiri schist belt located in the Anantapur district,Andhra Pradesh.The specific electrical conductiv-ity(EC) of these water samples was measured at 25℃ by using specific conductivity bridge.The specific conductiv-ity values of the water samples were also calculated by multiplying ionic concentrations with conductivity factor.It is found that the observed EC values of all water samples collected in both the seasons are different from the esti-mated EC values because of the electro-chemical properties of ions in water.  相似文献