Effects of UV-B irradiation on isoforms of antioxidant enzymes and their activities in red alga Grateloupiafilicina （Rhodophyta）   总被引：1，自引：0，他引：1  

赵吉强  李丽霞 《中国海洋湖沼学报》2014,32(6):1364-1372

Macroalgae in a littoral zone are inevitably exposed to UV-B irradiance.We analyzed the effects of UV-B on isoenzyme patterns and activities of superoxide dismutase(SOD),peroxidase(POX),catalase(CAT),and ascorbate peroxidase(APX) of red algae Grateloupia filicina(Lamour.) C.Agardh.The activities of SOD,CAT,and APX changed in response to UV-B in a time- and dose-dependent manner.POX activity increased significantly under all three UV-B treatments.The enzymatic assay showed three distinct bands of SODⅠ(Mn-SOD),SODⅡ(Fe-SOD),and SODⅢ(CuZn-SOD) under a low(Luv) and medium(Muv) dose of UV-B irradiation,while SODⅠ and SODⅢ activities decreased significantly when exposed to a high dose of UV-B irradiation(Huv).The activity of POX isoenzymes increased significantly after exposure to UV-B,which is consistent with the total activity.In addition,a clear decrease in activity of CATIV was detected in response to all the three doses of UV treatments.Some bands of APX isoenzyme were also clearly influenced by UV-B irradiation.Correspondingly,the daily growth rate declined under all the three exposure doses,and was especially significant under Muv and Huv treatments.These data suggest that,although the protection mechanisms of antioxidant defense system are partly inducible by UV-B to prevent the damage,G.filicina has incomplete tolerance to higher UV-B irradiation stress.  相似文献   

新疆沙冬青叶片蛋白质的双向电泳技术研究   总被引：1，自引：1，他引：0  

鲁春芳  尹林克 《中国沙漠》2011,31(1):96-100

提取新疆沙冬青叶片中蛋白质受到脂类、酚类等次生代谢产物的干扰,针对这种现象,进行了组分提取优化方法和双向电泳技术的改进。实验分析表明,用乙醚淋洗完整叶片,50 mmol·L-1 Tris-HCl缓冲液,上样量240 μg（7 cm胶条）,考马斯亮蓝染色等方法可很大程度增加提取可溶性蛋白含量,结合丙酮-三氯乙酸的沉降,可较完整地提取蛋白质组。  相似文献   

中肋骨条藻蛋白质双向电泳样品制备方法的比较     

王秀秀  陈纪新  黄邦钦 《海洋学研究》2012,30(4):72-77

比较了裂解液法和直接三氯乙酸(TCA)-丙酮沉淀法对中肋骨条藻Skeletonema costatum 蛋白双向电泳的提取效果并优化了提取条件,结果表明:裂解液-TCA-丙酮沉淀法在去除胞内干扰物质方面,较裂解液-超滤管法和裂解液-试剂盒法的效果都好。 裂解液-TCA-丙酮沉淀法和直接TCA-丙酮沉淀法都能取得背景干净、蛋白点清晰的双向电泳图谱,但后者的双向电泳图谱聚焦更完全,在进一步优化条件后(即蛋白沉淀12 h并增加超声波洗涤过程),可作为提取中肋骨条藻蛋白的最适方法。  相似文献   

First record of manta rays off New Zealand     

H. H. Gilbert  L. J. Paul 《新西兰海洋与淡水研究杂志》2013,47(2):339-342

Three manta rays were seen and photographed in the western Bay of Plenty, New Zealand, in March 1968. Although the species or genus could not be identified, this sighting represents the first substantiated record of the manta ray family (Mobulidae) in New Zealand and confirms the statements of fishermen that, in summer, mantas occasionally appear in offshore game‐fish waters in north‐eastern New Zealand.  相似文献   

Hauraki Gulf snapper fishery, 1972 and 1973: Some evidence for a declining catch‐rate     

L. J. Paul 《新西兰海洋与淡水研究杂志》2013,47(4):569-587

The commercial fishery for snapper Chrysophrys auratus (Bloch and Schneider) in the Hauraki Gulf is one of New Zealand's principal marine fisheries. The snapper catch from this area declined by 22% in 1972, and by a further 4% in 1973, following 4 y of high catches by Danish seiners.

Some of the decline can be attributed to economic factors within the industry, and adverse winter weather. However, the 1972 decline in Danish seine catches is an acceleration of an existing trend. It probably results from heavy exploitation of an accumulated stock, and from movement of a few strong year classes past the age at which they have contributed most to the catch. The slight recovery in the 1973 Danish seine catch resulted only from increased effort; catch per day at sea continued to fall. An improvement in catch and catch per day is unlikely for some years at least.

Snapper catches by other methods (trawl, long line, and set net) also declined during 1972, but the available data on fishing effort do not allow a detailed analysis. The trawl catch in the gulf fell further in 1973 as fishing effort was diverted to other grounds, but the catch by lines and nets improved slightly.  相似文献   

黄酮类化合物分析方法概述   总被引：9，自引：0，他引：9       下载免费PDF全文

赵晓莉  岳红 《盐湖研究》2005,13(2):34-39

主要综述了黄酮类化合物的分析方法。重点介绍了分光光度法、平面色谱法、高效液相色谱法、超临界流体色谱法、高效毛细管电泳法、极谱法、色谱—质谱联用技术。  相似文献   

三种鲀的毒性及其与种的相关性研究     

钟建兴朱金勇  许玉德 《台湾海峡》2003,22(2):168-172

用小鼠生物检测法及薄层等电聚焦电泳法测定3种河鲀的毒性及其蛋白质组分的种属差异性．结果表明，河鲀的毒性强弱与其种属有密切关系，不同种河鲀的毒性差异很大；同种河鲀的不同个体以及同一个体的不同器官组织也有较大差异．这些结果可为河鲀资源的开发利用提供实验依据。  相似文献   

利用毛细管电泳检测适冷蛋白酶自溶的动态过程     

孙彩云  陈秀兰  张玉忠  高培基 《海洋科学》2002,26(11):71-73

蛋白酶由于具有催化蛋白质水解的能力,因此在蛋白酶溶液中 ,蛋白酶分子可以相互催化,造成蛋白酶降解 ,酶活降低,这种现象称为蛋白酶自溶（ａｕｔｏｌｙｓｉｓ）。许多蛋白酶都具有自溶的特点。蛋白酶的自溶对蛋白酶的研究和保存带来很多困难。因此,弄清蛋白酶自溶的特点和机制非常重要 ,这是探讨防止蛋白酶降解方法的基础。Ｋｕｍｕｒａ等［４］对荧光假单胞菌产生的蛋白酶的自溶现象进行了研究,他们发现该蛋白酶在５０℃下 ,保温２ｍｉｎ就丧失９０%的活性。利用ＨＰＬＣ分离自溶片段进行Ｎ端测序表明 ,该酶的自溶酶切位点是不确定的 ,但靠…  相似文献   

荧光标记微卫星分析人工饲养中华绒螯蟹的遗传多样性   总被引：5，自引：1，他引：5  

朱泽远  王亚菊  施用晖  乐国伟 《中国海洋大学学报(自然科学版)》2007,37(4):591-596

分析中华绒螯蟹的遗传多样性，采集来自无锡、苏州和上海人工养殖样品102个，运用毛细管电泳检测荧光标记（FAM或HEX）的10个中华绒螯蟹微卫星DNA位点。在所有的3个群体中，单一位点等位基因数从15～42个，平均值为22．9；多态信息含量（PIC）和有效等位基因数（E）分别高达0．826和7．699。结果表明10个位点均为高度多态位点，中华绒螯蟹人工养殖群体具有较高的遗传多样性。欧氏遗传距离、遗传相似性和群体各亚群体内固定系数（Fst）平均值分别为0．439，0．825和0．099，结果显示不同的人工养殖群体之间也存在较大的遗传多样性。除上海群体在同步突变模式（SMM）下，近期不会有瓶颈（P=0．35）；所有群体在SMM和无限等位基因模式（IAM）下均有显著或极显著的瓶颈（P〈0．01或0．05），结果说明科学的品种保护计划的迫切性。本研究首次选择遗传标记微卫星DNA评估中华绒螯蟹的遗传多样性，并采用高精度的毛细管电泳检测技术，将对中华绒螯蟹的品种保护和合理利国提倡科学依据。  相似文献   

口虾蛄超氧化物歧化酶同工酶的组织特异性研究   总被引：7，自引：1，他引：7  

母昌考  王春琳  孙伟 《海洋湖沼通报》2003,(3):50-53

以口虾蛄为材料进行研究，用聚丙烯酰胺凝胶电泳法，对其肌肉、心脏、腹鳃、肝胰腺及眼球5种组织器官进行超氧化物歧化酶(SOD)同工酶的研究分析。结果表明：不同组织中超氧化物歧化酶(SOD)的同工酶谱带存在差异，具有明显的组织特异性。眼球、腹鳃、肝胰腺和心脏中有2条酶带，肌肉中几乎不表达，心脏中SOD2和肝胰腺中SODl谱带表达最浓，而腹鳃中的酶带表达最弱。  相似文献