Comparative analysis of four essential Gracilariaceae species in China based on whole transcriptomic sequencing     

XU Jiayue  SUN Jing  YIN Jinlong  WANG Liang  WANG Xumin  LIU Tao  CHI Shan  LIU Cui  REN Lufeng  WU Shuangxiu  YU Jun 《海洋学报(英文版)》2014,33(2):54-62

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Origin and evolution of alginate-c5-mannuronan-epimerase gene based on transcriptomic analysis of brown algae     

WANG Ren  WANG Xumin  ZHANG Yalan  YU Jun  LIU Tao  CHEN Shengping  CHI Shan 《海洋学报(英文版)》2014,33(2):73-85

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泥蚶(Tegillarca granosa)血细胞转录组序列中免疫相关基因的结构与mRNA表达量分析     

钟爱华  储张杰  王伟洪  牟毅 《海洋与湖沼》2020,51(2):354-363

贝类血细胞直接参与异物吞噬和包囊作用,还在伤口修复、炎症反应过程中发挥重要作用,是机体免疫防御的承担者。本研究采用RNA-seq测序技术研究了鳗弧菌感染前后泥蚶血细胞的转录组序列,通过序列组装,获得了α-葡萄糖苷酶(GAA)、α-甘露糖苷酶(LAMAN)、芳基硫酸酯酶B(ASB)、天冬酰胺氨基葡萄糖酶(AGA)、整合素β3亚基(ITB3)、热休克蛋白9(HSPA9)和Toll相互作用蛋白(TOLLIP)序列,利用Orffinder获得了其编码蛋白序列,并对蛋白序列进行了理化性质、二级结构、保守结构域和三级结构分析。结果表明:7种蛋白质二级结构均含有α螺旋、β折叠及无规则卷曲,泥蚶和人类ITB3和TOLLIP具有相同的保守结构域, Swiss-model同源建模获得的三级结构与模板同源性均超过35%(TOLLIP除外)。鳗弧菌刺激后,GAA、LAMAN、ASB、AGA和HSPA9的表达量显著增加,ITB3和TOLLIP表达量变化不明显。该研究补充和完善了对泥蚶免疫相关基因的认识,并为进一步开展泥蚶抗病机理研究提供了重要的理论依据。  相似文献   

Co-monitoring bacterial and dinoflagellates communities by denaturing gradient gel electrophoresis(DGGE) and SSU rRNA sequencing during a dinoflagellates bloom     

KAN Jinjun  CHEN Feng 《海洋学报(英文版)》2004,23(3):483-492

Dinoflagellates are unicellular eukaryotic protists that dominate in all coastal waters, and are also present in oceanic waters. Despite the central importance of dinoflagellates in global primary production, the relationship between dinoflagellates and bacteria are still poorly understood. In order to understand the ecological interaction between bacterial and dinoflageUates communities, denaturing gradient gel electrophoresis (DGGE) and SSU rRNA sequencing were applied to monitoring the population dynamics of bacteria and dinoflagellates from the onset to disappearance of a dinoflagellates bloom occurred in Baltimore Inner Harbor, from April 15 to 24, 2002. Although Prorocentrum minimum was the major bloom forming species under the light microscopy, DGGE method with dinoflagellate specific primers demonstrated that Prorocentrum micans, Gymnodinium galatheanum and Gyrodinium uncatenum were also present during the bloom. Population shifts among the minor dinoflagellate groups were observed. DGGE of PCR-amplified 16S rRNA gene fragments indicated that cyanobacteria, α, β, γ-proteobacteria, FlavobacteriumBacteroides-Cytophaga (FBC), and Planctomcetes were the major components of bacterial assemblages during the bloom. DGGE analysis showed that Cytophagales and α-proteobacteria played important roles at different stages of dinoflagellates bloom. DGGE can be used as a rapid tool to simultaneously monitor population dynamics of both bacterial and dinoflagellates communities in aquatic environments, which is demonstrated here.  相似文献   

中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究   总被引：3，自引：1，他引：3  

孔晓瑜  喻子牛  刘亚军  高天翔  武云飞 《中国海洋大学学报(自然科学版)》2001,31(6):861-866

以相应引物 PCR扩增了黄河口中华绒螯蟹线粒体细胞色素氧化酶 I亚基基因 (COI)片段 ,PCR产物经 T载体连接之后进行克隆、测序 ,得到 70 9bp的碱基序列 ,其 A,T,G,C含量分别为 34.4 1% ,2 7.93% ,2 0 .0 3%和 17.6 3%。并比较它与珠江流域中华绒螯蟹 COI序列和日本绒螯蟹 COI序列的差异 ,发现黄河口中华绒螯蟹与珠江流域中华绒螯蟹 COI序列完全相同 ,而与日本绒螯蟹差异非常明显 ,70 9或 6 5 8(不计引物 )位点中核苷酸差异数为 32 ,核苷酸差异率为 4 .5 1%或 4 .86 % (不计引物 ) ,其中 2 5个位点为转换 ,7个位点为颠换。作者倾向于支持存在中华绒螯蟹和日本绒螯蟹 ,或它们为同一种的两个地理亚种的观点  相似文献   

Heuristic approaches for mine planning and production scheduling   总被引：4，自引：0，他引：4  

Mark Gershon 《Geotechnical and Geological Engineering》1987,5(1):1-13

Summary Traditional methods and opimization methods for mine planning and production scheduling have failed to provide easy to use tools that yield improved results. Two new approaches, each combining the best of the traditional and the optimization, are described.  相似文献   

高等学校毕业生分配的模糊数学方法     

胡继才 《武汉大学学报(信息科学版)》1989,(2)

本文用模糊数学原理建立了高等学校毕业生分配工作的数学模型,其中各因素的权重排序由层次分析法确立。通过梯形分布建立模糊分布,排除了各方面人为因素的干扰,并用实例说明了这种方法的可行性和可靠性。  相似文献   

Plant responses to global change: next generation biogeography     

Jeremy S. Johnson  Keith D. Gaddis  David M. Cairns  Charles W. Lafon  Konstantin V. Krutovsky 《自然地理学》2016,37(2):93-119

Global change has become a major ecological concern that has the potential to dramatically alter plant distributions and assemblages worldwide. The specific response of plants to global change will depend on their ability to move, evolve, or adjust through phenotypic plasticity. Advances in biogeographic research are on the verge of a giant leap forward if we can take advantage of emerging tools in genetics and genomics. In this review, we appraise how new, post-Sanger, high-throughput sequencing and associated technologies can be used by next generation biogeographers to assess plant responses to global change. First, we briefly review the recent advances in genome sequencing and available approaches of genomic analysis. Secondly, we discuss the three main plant responses to global change: migration, adaptation, and phenotypic plasticity. This review is intended to spark the interest of biogeographers who do not traditionally use genetics but could benefit from including genetic and genomic tools into their research. Our goal is to illustrate that their use can contribute to new perspective and add an additional level of resolution into future biogeographic research.  相似文献   

Transcriptomic Analysis and Comparison of the Gene Expression Profiles in Fast- and Slow-Growing Pearl Oysters Pinctada fucata martensii     

XU Hanzhi  LIU Huiru  ZHANG Hua  HE Maoxian 《中国海洋大学学报(英文版)》2022,21(1):186-194

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青藏高原北麓河地区荒漠草原土壤细菌对热融滑塌的响应   总被引：2，自引：1，他引：1  

张晓兰  刘桂民  李新星  吴小丽  徐海燕  纪庚好  李莉莎  吴晓东 《冰川冻土》2019,41(4):977-985

多年冻土区地下冰融化造成的热融滑塌会对土壤理化性质产生一系列影响,进而影响微生物群落结构,但目前热融滑塌对荒漠草原区土壤微生物的影响还不清楚。利用Illumina测序方法,以青藏高原北麓河多年冻土区发生热融滑塌的荒漠草原为研究对象,对3种微地貌（对照区、滑塌区、沉降区）下的土壤细菌展开研究,分析了细菌群落结构及其与环境因子间的关系。结果显示滑塌区和沉降区有机碳含量显著低于对照区,滑塌区土壤含水量最高;放线菌门、变形菌门、绿弯菌门和酸杆菌门是3种微地貌下的优势菌群,对照区细菌群落丰富度显著低于滑塌区。Mantel检验表明,细菌群落结构与土壤理化性质间无显著相关性;非度量多维尺度分析（NMDS）表明,微生物群落结构在3种微地貌下显著不同。研究结果表明,在荒漠草原区,热融滑塌会改变土壤理化性质,影响细菌特定门的微生物相对丰度,但对门水平的整体群落结构影响不显著。  相似文献