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151.
152.
Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis) 总被引:3,自引:1,他引:2
A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus. 相似文献
153.
This article presents a method for the nonlinear analysis of laterally loaded rigid piles in cohesive soil. The method considers the force and the moment equilibrium to derive the system equations for a rigid pile under a lateral eccentric load. The system equations are then solved using an iteration scheme to obtain the response of the pile. The method considers the nonlinear variation of the ultimate lateral soil resistance with depth and uses a new closed‐form expression proposed in this article to determine the lateral bearing factor. The method also considers the horizontal shear resistance at the pile base, and a bilinear relationship between the shear resistance and the displacement is used. For simplicity, the modulus of horizontal subgrade reaction is assumed to be constant with depth, which is applicable to piles in overconsolidated clay. The nonlinearity of the modulus of horizontal subgrade reaction with pile displacement at ground surface is also considered. The validity of the developed method is demonstrated by comparing its results with those of 3D finite element analysis. The applications of the developed method to analyze five field test piles also show good agreement between the predictions and the experimental results. The developed method offers an alternative approach for simple and effective analysis of laterally loaded rigid piles in cohesive soil. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
154.
Glacigenic sediments exposed in coastal cliffs cut through undulatory terrain fronting the Last Glacial Maximum laterofrontal moraine at Waterville on the Iveragh Peninsula, southwest Ireland, comprise three lithofacies. Lithofacies 1 and 2 consist of interdigitated, offlapping and superimposed ice‐proximal subaqueous outwash and stacked sequences of cohesionless and cohesive subaqueous debris flows, winnowed lag gravels and coarse‐grained suspension deposits. These are indicative of sedimentation in and around small grounding line fans that prograded from an oscillating glacier margin into a proglacial, interlobate lake. Lithofacies 3 comprises braided river deposits that have undergone significant syn‐sedimentary soft‐sediment deformation. Deposition was likely related to proglacial outwash activity and records the reduction of accommodation space for subaqueous sedimentation, either through the lowering of proglacial water levels or due to basin infilling. The stratigraphic architecture and sedimentology of the moraine at Waterville highlight the role of ice‐marginal depositional processes in the construction of morphostratigraphically significant ‘end moraine’ complexes in Great Britain and Ireland. Traditional ‘tills’ in these moraines are often crudely stratified diamictons and gravelly clinoforms deposited in ice‐proximal subaqueous and subaerial fans. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
155.
吕言 《武汉大学学报(信息科学版)》1988,(4)
本文提出一种既可用于从图象上提取具有某种用途的一维边缘,又可用于提取具有多种用途的二维边缘的序贯一维型边缘检测新算法。由于构成该算法重要组成部分的基础算子的作用,以及将二维问题化为一维问题求解而使其明显具有简单,快速、有效的特点。该算法还具有Abdou等所建议的边缘检测算子应具有的两个特性。 相似文献
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157.
Gordon Kaufman 《Natural Resources Research》1994,3(4):304-314
The relative efficiencies of alternative geometric patterns of both discrete borehole and continuous grid line search have been extensively discussed in the mathematical geology literature. However, an equally important problem has received virtually no attention: How to use a sample of properties of geologic anomalies detected by grid line search of a region to estimate systematically both the number and size distribution of geologic anomalies missed by the search. We show how estimation methods developed in the sample survey design literature can be adapted to this problem, and we apply these methods to data describing 94 anomalies identified by a seismic reconnaissance survey. 相似文献
158.
Long-period surface waves and mantle boundary undulations 总被引:2,自引:0,他引:2
159.
地下管线探测的基本原理及解释方法研究 总被引:2,自引:0,他引:2
本文较详细地讨论了电磁直接探测法探测地下管线的基本原理和解释方法;提出了一种新的求解管线位置和深度、并给出探测结果可信度的向量交绘法。现场提供高可信度的结果;对管线探测仪器设备及应用效果作了介绍。 相似文献
160.