预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响     

姚敬元  刘瑀  李颖  王海霞 《海洋学报(英文版)》2016,35(6):1-6

This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae(Isochrysis zhanjiangenisis).To identify the effects of sample preparation on theδ~(13)C value and the fatty acid composition,we examined eight types of pretreatment methods including:(a) drying the sample followed by direct methyl esterification using HCl-CH_3OH;(b) drying the sample followed by direct methyl esterification using H_2SO_4-CH_3OH;(c) drying the sample by ultrasonic extraction and methylesterification using HCl-CH_3OH;(d) drying the sample by ultrasonic extraction and methyl-esterification using H_2SO_4-CH_3OH;(e) fresh sample followed by direct methyl-esterification using HCl-CH_3OH;(f) fresh sample followed by direct methyl-esterification using H_2SO_4-CH_3OH;(g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH_3OH,and(h) fresh sample with ultrasonic extraction followed by methylesterification using H_2SO_4-CH_3 OH.The results show that the δ~(13)C values from Groups a–e,g and h fluctuated within 0.3‰,and the δ~(13)C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.  相似文献   

风浪和海洋飞沫对海表面拖曳系数和风廓线的影响   总被引：2，自引：1，他引：1  

张婷  宋金宝  李爽  杨联贵 《海洋学报(英文版)》2016,35(11):79-85

基于埃克曼理论,本文将波致应力和飞沫应力引入到海-气边界层的界面应力中,来研究海表面风浪和海洋飞沫对海-气边界层动量交换的影响,并得到修改后的埃克曼模型的理论解。波致应力是由风浪谱和波增长函数估计,并得到在中低风速下,波致应力、飞沫应力与湍流应力相比,对海表面拖曳系数和风廓线的影响非常小。当风速高于25米/秒时,海洋飞沫通过飞沫应力对海-气界面应力的作用远高于波致应力,以至于波致应力可以忽略。海表面拖曳系数在高风速下,随着风速的增大而减小。通过采用风浪谱的不同波龄,得到海洋飞沫的产生会导致海-气边界层风速的增加。最后,理论解与现场的观察数据进行了对比。对比后的数据表明,在中高风速下,飞沫对海-气边界层的影响远大于表面风浪。  相似文献   

Monitoring the recovery of a previously exploited surf-zone fish community in the St Lucia Marine Reserve,South Africa,using a no-take sanctuary area as a benchmark     

BQ Mann  H Winker  JQ Maggs  SN Porter 《African Journal of Marine Science》2016,38(3):423-441

Limiting accessibility to a fishing area can reduce fishing effort effectively and may therefore promote local recovery of depleted stocks. In January 2002, beach driving was banned in South Africa, thereby reducing angler access to large areas of the coastline, particularly in less-developed areas. In November 2001 a project had been established in the St Lucia Marine Reserve, on the east coast of South Africa, to compare surf-zone fish populations inside a no-take sanctuary zone with those in an adjacent exploited area. Subsequent to the ban, the aim of the project was adapted such that surf-zone fish populations were monitored for potential recovery in what had been the exploited area, located to the north of Cape Vidal, which anglers could no longer access easily, because of the prohibition on beach driving. Standardised research fishing was conducted at two sites in the previously exploited area and two sites in the no-take sanctuary. Conventional stock-status indicators showed evidence of recovery in the four most common species caught in the previously exploited area, in terms of both abundance and biomass. Generalised additive mixed models were used to account for the influence of targeting specific species; however, subtle differences in habitat between the sampling sites, improved angling skill over time, variability in recruitment, and differential species-specific responses complicated interpretation of results. The implications of the findings are discussed in terms of the future monitoring and management of marine protected areas both within the St Lucia Marine Reserve and farther afield.  相似文献   

Structure and dynamics of demersal fish assemblages over three decades (1985–2012) of increasing fishing pressure in Guinea     

ML Camara  B Mérigot  F Leprieur  JA Tomasini  I Diallo  M Diallo 《African Journal of Marine Science》2016,38(2):189-206

In a context of growing fishing pressures and recommendations for an ecosystem approach to fisheries, there is a need to monitor changes in fish communities over time. In this study, we analysed data from scientific trawl surveys carried out on the continental shelf off Guinea between 1985 and 2012. We performed factorial analyses and calculated biodiversity indices to characterise the changes in the structure and composition of fish communities that occurred over the 28-year period in this area, particularly given intensive fishing activities. We show that, over the study period, fish communities on the Guinean shelf were structured primarily according to spatial factors, with temporal changes being less pronounced than expected. However, a temporal analysis of biodiversity indices and species dominance showed that the intensification of fishing had significant effects on the general ecological features of the fish assemblages under study. There was a decrease in fish density and in mean trophic level. In addition, there were changes in species dominance, whereby large, slow-growing species with high commercial value were gradually replaced by smaller, fast-growing species of lesser commercial interest. These results from field observations are in line with some conclusions from previous modelling studies in the same geographical area, and provide further support for the hypothesis of a progressive ‘ecosystem effect of fishing’ occurring in Guinean waters.  相似文献   

福建兴化湾中部表层海水及沉积物中重金属含量特征与生态风险评价          下载免费PDF全文

王伟力  王继纲  林彩  刘洋  林辉 《应用海洋学学报》2016,35(2):284-293

分析研究了兴化湾中部表层海水Cu、Pb、Zn、Cd、Cr、Hg、As的含量发现,表层海水中Cu、Pb、Zn、Cd、Cr、Hg、As含量均符合海水水质一类标准,对表层沉积物中Cu、Pb、Hg、Cr、Cd、As等6种重金属含量分析表明,6种重金属含量均符合海洋沉积物质量一类标准.表层海水中重金属与环境因子相关性分析表明,6种重金属与环境的相关性在不同季节中存在差异;而表层沉积物中重金属与环境因子的分析表明,重金属具有相近的来源,有机质对沉积物中重金属的含量分布具有重要影响.主成分分析结果表明,兴化湾中部表层沉积物7种重金属主要来源为有机质的降解、沉积环境本身及工业排污的影响,其贡献率分别为59.48%、15.30%、10.98%,3个主成分的贡献率合计达到85.76%.潜在生态危害指数法评价结果表明,Cu、Pb、Zn、Cd、Cr、Hg、As均属轻微生态危害.  相似文献   

扩散型海洋倾倒区容量估算方法初探——以嵊泗上川山疏浚物海洋倾倒区为例          下载免费PDF全文

何东海  龙绍桥  辛士河  江志法  沈继平  郭清荣 《应用海洋学学报》2016,35(2):294-301

以嵊泗上川山疏浚物海洋倾倒区为例,探讨扩散型海洋倾倒区容量.通过生态敏感目标水质保护要求来确定倾倒活动最大倾倒强度;以最大倾倒强度及倾倒区本身和周边海域港航功能正常发挥为前提,确定水深可淤积厚度,以此为基础,分析系列倾倒量下的区域淤积影响程度,最终确定可接受情况下的倾倒量,从而估算倾倒容量.倾倒活动产生的悬浮物影响对生态敏感目标影响分析,倾倒活动瞬时最大可接受倾倒强度至少为36 000 m3(基于实际工况);在7个月倾倒期内,设定1 500×104、4 000×104、6 000×104m3系列倾倒量,分析倾倒区本身及周边水深敏感目标的淤积程度,最终确定在1 500×104m3倾倒量下,淤积程度可接受,初步估算该倾倒区的容量为1 500×104~2 000×104m3.  相似文献   

1311号台风“尤特”水汽和螺旋度分析          下载免费PDF全文

覃昌柳  黎惠金  张丁丁 《应用海洋学学报》2016,35(4):459-468

通过NCEP再分析资料和Micaps实况资料对"尤特"发生发展以及登陆过程的水汽条件、螺旋度变化进行分析,发现:"尤特"在发生发展前期,西南急流和副高南侧偏东气流对"尤特"水汽输送作用相当,中后期"尤特"则主要以西南急流形成的南支水汽通道输送为主,水汽的源地主要来自南海.对于广西强降雨区,在"尤特"进入广西前,该区为一个水汽源,南边界水汽输出显著,而到了15~19日主要降雨发生时段,受南支水汽输送带的影响,广西强降雨区转为水汽汇,水汽输入以南边界为主.环境场水汽通量和水汽通量散度的变化对"尤特"强度的变化和移向有一定的指示作用,"尤特"在加强或减弱前,其中心水汽通量往往先加强或减弱,且在台风维持一定的强度时,"尤特"有趋向周边水汽大值中心移动的趋势."尤特"水汽辐合主要位于低层,其强降雨区与850~950h Pa水汽通量散度场、850h Pa螺旋度相对大值区内有一定的对应关系,未来6h强降雨区多出现在低层强度强、垂直伸展高度高的水汽辐合区和螺旋度相对大值区内.  相似文献   

两种海洋微藻的浓度和配比对太平洋纺锤水蚤生长的影响          下载免费PDF全文

付聪  吴荔生  郭东晖  王桂忠 《应用海洋学学报》2016,35(4):537-543

本研究以变态存活率、产卵量、成体头胸部长和体长为指标,研究了球等鞭金藻(Isochrysis galbana)和海洋原甲藻(Prorocentrum micans)不同浓度和配比对太平洋纺锤水蚤(Acartia pacifica)生长发育和产卵量的影响,以期探索培养太平洋纺锤水蚤最适合的饵料配方.实验结果表明:在饵料浓度(碳含量,下同)为1.70μg/cm3,配比为2∶1时,其变态存活率最高,可达到92.62%;在饵料浓度为4.25μg/cm3,配比为1∶2时,产卵量最高,雌体平均日产卵量可达47.1±2.7个;在饵料浓度为1.70μg/cm3,配比为2∶1时,成体头胸部长和体长均最长,分别为932.9、1 252.3μm.太平洋纺锤水蚤生长和发育的最优饵料组合为球等鞭金藻与海洋原甲藻浓度为1.70μg/cm3,配比为2∶1.  相似文献   

定量模拟分析船体与空气枪间的安全距离          下载免费PDF全文

王立明  胡毅  许江  刘保华 《应用海洋学学报》2016,35(4):575-578

海洋地震勘探过程中,水中放置的空气枪与船体之间须保持一定安全距离,否则引起船载设施和仪器剧烈抖动,影响船舶设施和仪器的安全运行,甚至更严重情况下,有可能造成船体的损坏.利用海水压力脉冲与船体受到的冲击压力关系,结合建立的空气枪震源子波模型模拟近场震源子波,可计算出船体与空气枪间的安全距离.研究结果表明,根据震源子波模型模拟的近场子波主峰值,总容量150in3G枪、300 in3相干G枪及760 in3相干G枪的安全距离分别为3.90、6.57 m和9.13 m.由此可知,空气枪与船体间距离只有大于安全距离,才能避免船体大的振动或设备免遭损害,保证海洋地震勘探有效、安全的进行.  相似文献   

超大海洋保护区建设利弊分析          下载免费PDF全文

章飞军  应岳  蒋红  叶慧明  叶属峰  邹德云 《海洋开发与管理》2016,33(3):77-82

近年来,海洋保护区被不断的"巨型化",这一方面显示了国际社会对海洋保护的重视;另一方面,这股建设超大海洋保护区的浪潮也受到了很多科学工作者的质疑和批判。文章通过分析整理国内外相关文献,结合目前全球几个著名的超大海洋保护区案例,评价建设超大海洋保护区的利弊,并总结出我国海洋保护区建设可借鉴的经验。  相似文献