Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis     

H. Jonsson  K. Viken Sandnes  D. Schiedek  R. Schneider  B. E. Grsvik  A. Goksyr 《Marine environmental research》2004,58(2-5):655

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.  相似文献   

A note on estimation of the Jacobian elliptic parameter in cnoidal wave theory     

Y. -S. Cho   《Ocean Engineering》2003,30(15):1915-1922

A new and simple calculating technique for the Jacobian elliptic parameter is presented in this study. The technique is very useful in generating a train of cnoidal waves in both laboratory and numerical wave tanks. Upon specification of water depth, the wave height and either the wave period or the wavelength, the proposed technique uses the Newton–Raphson method to estimate the Jacobian elliptic parameter directly, without trial and error procedures or look-up in tables. It is shown that the technique provides equally accurate results as the ad hoc methods previously used.  相似文献   

基于EOF分析对南海西北海域水体光谱特性的研究   总被引：1，自引：0，他引：1  

付东洋  黄宇业  刘大召  廖珊  余果  张小龙 《海洋学报(英文版)》2020,39(7):107-114

This study presents an analysis of the spectral characteristics of remote sensing reflectance(R_(rs)) in northwestern South China Sea based on the in situ optical and water quality data for August 2018.R_(rs)was initially divided into four classes,classes A to D,using the max-classification algorithm,and the spectral properties of whole R_(rs) were characterized using the empirical orthogonal function(EOF) analysis.Subsequently,the dominant factors in each EOF mode were determined.The results indicated that more than 95% of the variances of R_(rs) are partly driven by the back-scattering characteristics of the suspended matter.The initial two EOF modes were well correlated with the total suspended matter and back-scattering coefficient.Furthermore,the first EOF modes of the four classes of R_(rs)(A-D R_(rs)-EOF_1) significantly contributed to the total variances of each R_(rs) class.In addition,the correlation coefficients between the amplitude factors of class A-D R_(rs)-EOF_1 and the variances of the relevant water quality and optical parameters were better than those of the unclassified ones.The spectral shape of class AR_(rs)-EOF_1 was governed by the absorption characteristic of chlorophyll a and colored dissolved organic matter(CDOM).The spectral shape of class B R_(rs)-EOF_1 was governed by the absorption characteristic of CDOM since it exhibited a high correlation with the absorption coefficient of CDOM(a_g(λ)),whereas the spectral shape of class C R_(rs)-EOF_1 was governed by the back-scattering characteristics but not affected by the suspended matter.The spectral shape of class D R_(rs)-EOF_1 exhibited a relatively good correlation with all the water quality parameters,which played a significant role in deciding its spectral shape.  相似文献   

The Bering Sea—A dynamic food web perspective     

Kerim Aydin  Franz Mueter   《Deep Sea Research Part II: Topical Studies in Oceanography》2007,54(23-26):2501

The Bering Sea is a high-latitude, semi-enclosed sea that supports extensive fish, seabird, marine mammal, and invertebrate populations and some of the world's most productive fisheries. The region consists of several distinct biomes that have undergone wide-scale population variation, in part due to fisheries, but also in part due to the effects of interannual and decadal-scale climatic variation. While recent decades of ocean observation have highlighted possible links between climate and species fluctuations, mechanisms linking climate and population fluctuations are only beginning to be understood. Here, we examine the food webs of Bering Sea ecosystems with particular reference to some key shifts in widely distributed, abundant fish populations and their links with climate variation. Both climate variability and fisheries have substantially altered the Bering Sea ecosystem in the past, but their relative importance in shaping the current ecosystem state remains uncertain.  相似文献   

真鲷的营养需要及配合饲料的研究初报   总被引：8，自引：0，他引：8  

刘竹伞  周海涛 《海洋湖沼通报》1994,(4):48-55

本文综述了真鲷的营养需要，研究了不同植物蛋白源对真鲷生长的影响，并在此基础上设计了三种饲料配方，试验结果表明Ｉ号饲料配方的增长率最高。  相似文献   

白沙河地下水资源管理模型     

刘贯群  邱汉学  焦超颖 《中国海洋大学学报(自然科学版)》1994,(Z3)

针对白沙河地下水含水层的内部结构和本区开采特点以及已出现的环境问题，建立了地下水资源管理模型，确定了优化开采方案。该管理模型针对本区每年２个管理时段的长度及其开采量均不同的特点，导出了新的响应矩阵的公式。管理模型的建立有助于对该区地下水的合理开采。  相似文献   

吉尼系数的计算及正态分布的吉尼系数     

陈峥  刘宝生  孙祥林 《中国海洋大学学报(自然科学版)》2002,32(4):663-666

本文给出了吉尼系数的一般计算公式 ,推导出正态分布的吉尼系数渐进无偏估计 ,并以实例对比了传统的吉尼系数的算法——万分法与本文中提出的估计方法。  相似文献   

建立高程异常内插计算综合模型的研究     

刘麦喜  隋立芬  吴延锋 《海洋测绘》2007,27(5):8-10

大地测量中数值逼近模型可分为两类:函数模型与统计模型,两种类型各有优、缺点。函数模型逼近一般求定逼近场的系统性或某种规律性趋势。统计模型逼近的主要特点是计算灵活,尤其对稳态随机过程的逼近效果较好。试图将二者有机地结合起来,以便充分利用函数模型逼近的规律性和统计模型逼近的灵活性,从而提高待求量的精度和可靠性。并通过实际算例证明将两者结合起来可有效改善拟合效果。  相似文献   

Measurements of higher harmonic wave forces on a vertical truncated circular cylinder     

S.Y. Boo   《Ocean Engineering》2006,33(2):219-233

Wave forces on a vertical truncated circular cylinder in Stokes waves with the wave slopes ranging from 0.06 to 0.24, are measured in a wave tank. The higher harmonic wave forces are compared with the available values from theories of the FNV (Faltisen–Newman–Vinje) model and Varyani solution. The first harmonic horizontal forces measured are much larger than the theoretical values from the FNV model, while the first harmonic vertical forces are well predicted by the Varyani theory. It was also found that the FNV model significantly overpredicts the second harmonic horizontal forces in high frequency waves, but under predicts the third harmonic forces. The differences between the actual measurement and the theory, in the second and third harmonic horizontal forces, become smaller at low wave frequencies as the wave slope increases. In addition, the transverse instabilities in the incoming waves with high wave slope were observed, which is due to the nonlinear modulation. Measurements were, thus, carried out before the instability occurred.  相似文献   

关于拟共形映照Mori常数的注记     

姚增善 《中国海洋大学学报(自然科学版)》1996,(4)

讨论拟共形映照的偏差问题，加强关于拟共形映照Ｈｏｌｄｅｒ连续性的Ｍｏｒｉ偏差定理  相似文献