盐田中嗜盐菌的初步研究   总被引：4，自引：0，他引：4  

吕爱军  刘缠民  华栋  屈艾  朱必才 《海洋科学》2003,27(12):5-6

在连云港台南盐场盐田中取卤水和底泥样品，以盐(NaCl)为限制性生长因子，主要采用12％NaCl平板，对样品中的嗜盐菌进行初步鉴定和培养计数研究。结果表明，盐田中的嗜盐菌类群在菌落、个体特征以及数量分布上有所不同，并且底泥中的菌量比卤水中高1～2个数量级。  相似文献   

秦山核电站邻近水域石油降解细菌的生态学研究     

陈忠元  史君贤 《海洋学研究》1991,9(2):48-53

本文分析了1989年4月(春),7月(夏),10月(秋)和1990年1月(冬)秦山邻近水域表层水中石油降解细菌的丰度和分布规律,同时比较了温度、含油量、营养盐、溶解氧、盐度等环境因素对其数量变动的影响。分析结果发现,测区表层水中石油降解菌数量最高值达1.1×10~5个/dm~3,最低值为400个/dm~3,季节变化波动于97～4.9×10~4个/dm~3之间,菌量变化与水温,含油量呈正相关,与盐度呈负相关趋势,认为测区高营养盐、高溶解氧状况可能不成为石油降解细菌生长繁殖的限制因子,并指出测区低丰度的石油降解菌数量与该海区低水平的油污是一致的。  相似文献   

红色无硫细菌处理高浓度鱼品废水的研究   总被引：1，自引：0，他引：1  

姜秀萍  陈世阳 《中国海洋大学学报(自然科学版)》1989,(Z2)

本文从城市生活污水的沉降污泥中分离出三株红色无硫细菌,经鉴定R1为胶质红假单胞菌(Rhodopscudomonas gelatinosa),R2为沼泽红假单胞菌(R palustris)、RS-1为红螺属(Rhodosprillum)的一种。R1与R2均为兼性需氧和兼性光能利用菌,RS-1为专性厌氧光合菌。作者提出了它们的主要生理、生化特性和最适生长条件,最后确定R1、R2是较有利用前途的优势种类。作者利用上述分离的菌株对高浓度的鱼品废水进行了净化处理试验,得出R1对CODcr的去除率约为65％,R2的去除率为74％以上。二者混合使用可提高至87％。作者还发现废水中的非光合性杂生菌有助于提高光合细菌的净化力。  相似文献   

两株琼胶酶高产细菌的筛选和鉴定   总被引：4，自引：1，他引：4  

杜宗军  王鹏  李筠  P.A.W.Robertson  B.Austin 《海洋科学》2002,26(3):1-4

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。  相似文献   

Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay     

Akira Shibata  Kouichi Ohwada  Masakazu Tsuchiya  Kazuhiro Kogure 《Journal of Oceanography》2006,62(1):91-97

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.  相似文献   

添加维生素B_（12）生产菌高密度培养褶皱臂尾轮虫的效果     

于建平  日野明德 《中国海洋大学学报(自然科学版)》1994,(2)

阐述把维生素Ｂ１２生产菌添加到培养水中培养褶皱臂尾轮虫ＢｒａｃｈｉｏｎｕｓＰｌｉｃａｔｉｌｉｓ的买验。共１８株细菌分离于轮虫培养池，其中，有一株产维生素Ｂ１２的假单胞杆菌ＴＰ４对轮虫的生长繁殖有明显的促进作用。把ＴＰ４菌株培养后，加入到２Ｌ的烧杯和５００Ｌ的水槽中培养泰国Ｓ型轮虫时．在９ｄ（天）和６ｄ（天）中，轮虫密度从１２４～１３９和２４２～２８８个体／ｍｌ增殖到４，４１７～５，５４０和１，０１７～１，２５４个体／ｍｌ，分别比对照组增加了４～６及２～３倍。  相似文献   

Effects of an Artificial Breakwater on the Distributions of Planktonic Microbial Communities     

Young-Ok Kim  Eun Jin Yang  Jung-Hoon Kang  Kyoungsoon Shin  Man Chang  Cheol Soo Myung 《Ocean Science Journal》2007,42(1):9-17

The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L¹) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed into more than 10⁶ cells L¹ at the surface layer of the inner area, while its abundance was much lower (10³-10⁴ cells L¹) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p < 0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates.  相似文献   

胶州湾异养细菌及大肠菌群的分布及对陆源污染的指示     

赵三军  肖天  李洪波  徐剑虹 《海洋与湖沼》2005,36(6):541-547

2002年2月—2004年11月对胶州湾异养细菌和大肠菌群进行长期调查发现,胶州湾异养细菌丰度在105—106cells/ml之间,其中河口区域和近岸区域数量较高,其它区域相对较低。从季节上来看,夏季、秋季细菌丰度较高(分别为7.4×105、7.2×105cells/ml),冬季和春季丰度较低(分别为4.1×105cells/ml、5.9×105cells/ml),且在一年内呈现出一定的规律性波动。从季节尺度上看异养细菌丰度与温度呈现很好的正相关性(R=0.54)。对大肠菌群的研究表明,河口区域的大肠菌群数量显著高于其它区域,并且河口区域大肠菌群常年处于严重超标状态(最高为1.1×106cells/L,超过国家前三类水质标准的100倍)。根据大肠菌群的数量状况可以将胶州湾分为三个区域,即严重污染区、中度污染区和轻度污染区。该区域划分与通过营养盐对胶州湾区域的划分(富营养区、中度营养区、贫营养区)结果一致。  相似文献   

Microbial Biomass Dynamics Along a Trophic Gradient at the Atlantic Barrier Reef off Belize (Central America)   总被引：2，自引：0，他引：2  

Gerhard J.  Herndl 《Marine Ecology》1991,12(1):41-51

Abstract. Recent findings indicate that heterotrophic bacteria and not phytoplankton are the most numerous biomass components even in the euphotic zone of oligotrophic, open oceans. In this study it was hypothesized that the microbial biomass components change within a few hundred meters as oligotrophic water flows across the reef and becomes enriched with nutrients. Along a trophic gradient, four stations at the Atlantic Barrier Reef off Belize (Central America) were sampled for microbial biomass components. Phytoplankton biomass (measured as chlorophyll a) ranged from the most oligotrophic station (St. 1) to the most eutrophic station (St. 4) from 6.9–415.5 μg CI"' (assuming a C:chl a ratio of 30): heterotrophic bacterial biomass increased 4-fold (from 10.1–46.4μg C 1^-1), heterotrophic nanoflagellate (HNAN) biomass increased from 4.6-19ug C 1^-1, and cyanobacteria from 0.9-4.5 μg C-1^-1. Production estimates derived from seawater cultures revealed a 5-fold increase in bacterial production from the oligotrophic station (3.7 ug C 1^-1 d^-1) to the eutrophic St. 4 (17.8ug C-1-d1^-1)- Cyanobacterial production rose from 1.1–3.5ug C-1–d^-1 and HNAN production from 0.65-1.13 μg C-1^-1 -d^-1. While cyanobacteria contributed between 13 and 20% to the autotrophic plankton component in the oligotrophic waters, their contribution dropped to about 1 % at the eutrophic stations.  相似文献   

中国对虾丝状细菌的扫描电镜研究     

杨季芳  吴友吕 《海洋学研究》1992,10(4):56-62

本文应用扫描电镜研究了中国对虾丝状细菌的结构,附着方式及其对对虾的危害和与环境的关系。  相似文献