胶州湾沉积物中石油降解菌的降解能力及多样性研究     

高伟  高祥兴  米铁柱  韩彬  张毅然  李馨子  尹晓斐  孙承君  李倩  崔志松  栾晓  于志刚  郑立 《海洋学报(英文版)》2019,38(6):54-64

2013年青岛输油管道爆炸，大量石油污染了附近海岸。课题组采集了污染的沉积物样品，以原油为唯一碳源和能源，富集了四个石油降解菌群。生物多样性和群落分析表明，Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌，都属于变形菌门。从石油降解菌群中分离筛选，获得了9株具有不同16S rRNA基因序列的降解菌，分别属于8个属。重量法测定降解菌的石油降解率，其中5株的石油降解率大于30%。GC-MS分析结果表明，石油降解菌多倾向于降解烷烃，对多环芳烃的降解能力较差，其中5株细菌的烷烃降解率较大，仅1株菌D2对多环芳烃的降解率较大，其降解率在34.9%到77.5%。通过对高通量数据的分析，表明食烷菌属是菌群A和菌群E的主要降解菌群，其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力，为油污染海滩生物修复提供了菌株资源。  相似文献   

淡化浓海水日晒盐田原核微生物多样性分析     

秦乐宁  黄鑫洁  朱大玲  侍亚敏  唐娜  王学魁 《海洋科学》2019,43(5):27-35

海滨日晒盐田具有稳定且独特的生态系统。淡化浓海水排入盐田制盐,对盐田生态系统的稳定及盐田的可持续发展具有一定的影响。本研究分析天津汉沽淡化浓海水日晒盐田不同季节不同盐度盐池原核微生物多样性现状及群落结构变化,以期为评估淡化浓海水对盐田原核微生物群落影响提供理论依据。采用温度梯度凝胶电泳和序列测定等方法,分析不同月份不同盐度盐池中细菌和古菌的多样性与群落结构变化。分别采用香农-威纳指数、加权丰富度指数和均匀度指数分析盐田微生物群落多样性、丰富度和均匀度。结果表明,淡化浓海水和溴后水中均未检测出细菌和古菌。不同月份不同盐度盐池样本中细菌多样性变化不大,优势菌主要为γ-变形菌门(γ-Proteobacteria)中的Spiribacter salinus和Pseudoalteromonassp.;古菌仅出现在盐度最高的Ⅶ号盐池中,优势古菌为广古菌门(Euryarchaeota)的Halogeometricum sp.。天津汉沽淡化浓海水日晒盐田中细菌的多样性和丰富度较低,春夏两季细菌群落结构较为稳定,秋冬季节细菌群落结构变化明显。盐度不同的盐池中细菌群落结构变化较大,随着盐度逐渐升高, Spiribacter salinus逐渐取代Pseudoalteromonas sp.成为占主要地位的优势菌种。  相似文献   

Response of Microbial Communities in Soil to Multi-level Warming in a Highland Barley System of the Lhasa River     

FU Gang  SUN Wei  LI Shaowei  ZHONG Zhiming 《资源与生态学报(英文版)》2019,10(4):373-378

No studies have examined the effect of experimental warming on the microbial biomass and community composition of soil in agricultural ecosystem on the Qinghai-Tibet Plateau. Thus it is unclear whether the influences of experimental warming on microbial communities in soil are related to warming magnitude in croplands on this Plateau. This study performed warming experiment (control, low- and high-level) in a highland barley system of the Lhasa River in May 2014 to examine the correlation between the response of microbial communities in soil to warming and warming magnitude. Topsoil samples (0-10 and 10-20 cm) were collected on September 14, 2014. Experimental warming at both low and high levels significantly increased soil temperature by 1.02 ℃ and 1.59 ℃, respectively at the depth of 15 cm. Phospho lipid fatty acid (PLFA) method was used to determine the microbial community in soil. The low-level experimental warming did not significantly affect the soil’s total PLFA, fungi, bacteria, arbuscular mycorrhizal fungi (AMF), actinomycetes, gram-positive bacteria (G⁺), gram-negative bacteria (G^-), protozoa, the ratio of fungi to bacteria (F/B ratio), and ratio of G⁺ to G^- (G⁺/G^- ratio) at the 0-10 and 10-20 cm depth. The low-level experimental warming also did not significantly alter the composition of microbial community in soil at the 0-10 and 10-20 cm depth. The high-level experimental warming significantly increased total PLFA by 74.4%, fungi by 78.0%, bacteria by 74.0%, AMF by 66.9%, actinomycetes by 81.4%, G⁺ by 67.0% and G^- by 74.4% at the 0-10 cm depth rather than at 10-20 cm depth. The high-level experimental warming significantly altered microbial community composition in soil at the 0-10 cm depth rather than at 10-20 cm depth. Our findings suggest that the response of microbial communities in soil to warming varied with warming magnitudes in the highland barley system of the Lhasa River.  相似文献   

External controls on the distribution,fabrics and mineralization of modern microbial mats in a coastal hypersaline lagoon,Cayo Coco (Cuba)   总被引：1，自引：0，他引：1       下载免费PDF全文

Anthony Bouton  Emmanuelle Vennin  Aurélie Pace  Raphaël Bourillot  Christophe Dupraz  Christophe Thomazo  Arnaud Brayard  Guy Désaubliaux  Pieter T. Visscher 《Sedimentology》2016,63(4):972-1016

Active, carbonate‐mineralizing microbial mats flourish in a tropical, highly evaporative, marine‐fed lagoonal network to the south of Cayo Coco Island (Cuba). Hypersaline conditions support the development of a complex sedimentary microbial ecosystem with diverse morphologies, a variable intensity of mineralization and a potential for preservation. In this study, the role of intrinsic (i.e. microbial) and extrinsic (i.e. physicochemical) controls on microbial mat development, mineralization and preservation was investigated. The network consists of lagoons, forming in the interdune depressions of a Pleistocene aeolian substratum; they developed due to a progressive increase in sea‐level since the Holocene. The hydrological budget in the Cayo Coco lagoonal network changes from west to east, increasing the salinity. This change progressively excludes grazers and increases the saturation index of carbonate minerals, favouring the development and mineralization of microbial mats in the easternmost lagoons. Detailed mapping of the easternmost lagoon shows four zones with different flooding regimes. The microbial activity in the mats was recorded using light–dark shifts in conjunction with microelectrode O₂ and HS^? profiles. High rates of O₂ production and consumption, in addition to substantial amounts of exopolymeric substances, are indicative of a potentially strong intrinsic control on mineralization. Seasonal, climate‐driven water fluctuations are key for mat development, mineralization, morphology and distribution. Microbial mats show no mineralization in the permanently submersed zone, and moderate mineralization in zones with alternating immersion and exposure. It is suggested that mineralization is also driven by water‐level fluctuations and evaporation. Mineralized mats are laminated and consist of alternating trapping and binding of grains and microbially induced magnesium calcite and dolomite precipitation. The macrofabrics of the mats evolve from early colonizing Flat mats to complex Cerebroid or Terrace structures. The macrofabrics are influenced by the hydrodynamic regime: wind‐driven waves inducing relief terraces in windward areas and flat morphologies on the leeward side of the lagoon. Other external drivers include: (i) storm events that either promote (for example, by bioclasts covering) or prevent (for example, by causing erosion) microbial mat preservation; and (ii) subsurface degassing, through mangrove roots and desiccation cracks covered by Flat mats (i.e. forming Hemispheroids and Cerebroidal structures). These findings provide in‐depth insights into understanding fossil microbialite morphologies that formed in lagoonal settings.  相似文献   

广西隆安下石炭统都结微生物岩微相特征与沉积环境研究     

苗卓伟  张永利  巩恩普  关长庆  阮氏梅香  黄文韬  王邓 《地质论评》2016,62(5):1134-1148

广西隆安县都结乡发育的微生物岩产于下石炭统都安组中—上部地层,分布广、厚度大,是中国南方早石炭世报道较少的碳酸盐岩建造类型,具有较高的研究价值。该微生物岩以颗粒灰岩和粘结岩为主,生物含量丰富,包括微生物、有孔虫、棘皮类、钙球、腕足等。通过岩相分析,可识别出以下8种微相类型:MF1含亮晶胶结物的泥晶包壳生屑灰岩、MF2含腕足似球粒灰岩、MF3无纹层似球粒颗粒灰岩、MF4集合颗粒灰岩、MF5细密纹层状粘结岩、MF6内碎屑鲕粒灰岩、MF7鲕粒灰岩、MF8白云质鲕粒灰岩。伴随海平面升降变化,都结微生物岩沉积环境先后经历了FZ1台地边缘浅滩相、FZ2开阔台地相与FZ3局限台地相这三种沉积相带之间往复变化的沉积相模式。结合前人基础地质资料,该沉积相模式表明该微生物岩发育于低纬度孤立碳酸盐岩台地浅水环境,并经历了初期、主体时期和消亡期3个发育阶段,海平面虽伴有小幅波动,但整体呈下降趋势。微生物岩发育于晚古生代冰期与谢尔普霍夫生物灭绝事件前夕,该时期南盘江盆地微生物岩的富集与后生动物礁的缺失相耦合,海洋生态系统中生物组成发生显著变化。在此基础上,本文从微生物岩的角度初步探讨了冈瓦纳大陆冰川作用对北半球低纬度地区的影响。  相似文献   

桂林毛村不同植被类型土壤微生物数量与碳酸酐酶活性比较     

黄芬  黄艳梅  杨利超  白冰  曹建华 《地质论评》2016,62(1):94-102

以非岩溶区林地为对比,分析了桂林毛村岩溶区4种不同植被类型土壤微生物数量及碳酸酐酶(CA)活性的季度动态变化规律,发现以下主要结果:1随着植被的正向演替,岩溶区弃耕地、草地、灌丛及林地微生物数量及CA活性逐渐升高,微生物总数从64.07×10~4cfu/g上升到178.23×10~4cfu/g,CA活性从0.77 U/g上升到1.82 U/g,岩溶区林地大于非岩溶区林地。2在岩溶区不同植被类型,微生物组成均表现为细菌最多(平均值95.14%),放线菌次之(平均值2.79%),真菌数量最少(平均值1.75%)。而在非岩溶区表现为细菌最多(平均值90.95%),真菌次之(平均值5.32%),放线菌最少(平均值3.73%)。3微生物数量季节动态整体表现为春季至夏季逐渐上升,至秋季达到最高,冬季下降,微生物总数的增长依赖于细菌的倍数增长,真菌和放线菌影响较小。CA活性整体表现为夏季和冬季低于春季和秋季,秋季达到最大值。4 CA活性与细菌及微生物总数呈极显著的正相关,表明土壤CA主要来源于细菌的分泌。  相似文献   

我国砂岩型铀矿分带特征研究现状及存在问题   总被引：1，自引：0，他引：1  

付勇  魏帅超  金若时  李建国  奥琮 《地质学报》2016,90(12):3519-3544

作为一种重要的国家战略资源,砂岩型铀矿床是当今世界上最重要的铀矿床类型之一。本文详细地介绍了砂岩型铀矿在国内外的分布特征及占比情况,并对外生地质作用矿床类型中表生流体作用形成的层间渗透砂岩型和潜水渗透砂岩型铀矿床进行了讨论,发现层间渗透砂岩型铀矿床在外表颜色、矿物组合以及地球化学等方面均具有明显的氧化-还原分带现象,此外,矿床内部还具有细菌分带现象。颜色分带在氧化带、氧化-还原过渡带以及还原带之间具有明显不同的特征;矿物组合在不同分带之间各不相同;地球化学分带表现为U、TOC含量以及Fe~(2+)/Fe~(3+)、Th/U比值在各分带之间差异较大。此外,硫酸盐还原菌、硫杆菌、铁细菌及硝化菌等细菌在不同分带之间的数量相差悬殊,而且硫酸盐还原菌数量与TOC呈明显正相关性。通过矿化带内的碳、硫同位素分析,发现硫酸盐还原菌参与了成矿过程,推测其可能是导致碳、硫同位素分馏的主要因素。总体来看,颜色分带、矿物分带、地球化学分带以及细菌分带均与氧化-还原分带呈耦合关系。本文通过总结层间渗透砂岩型和潜水渗透砂岩型铀矿床的成矿模式和当前分带研究中存在的问题,提出了由细菌、地球化学反应参与的砂岩型铀矿床成矿机理,以及未来亟需解决的若干关键科学问题。典型砂岩型铀矿床的分带现象在物、化、探、遥等领域的异常响应对寻找砂岩型铀矿床具有重要的指导意义。  相似文献   

A seasonal comparison of prokaryote numbers,biomass and heterotrophic productivity in waters of the KwaZulu-Natal Bight,South Africa     

D Muir  T Kunnen 《African Journal of Marine Science》2016,38(1):S123-S138

The KwaZulu-Natal Bight is a shallow indentation of the eastern seaboard of South Africa, characterised by a narrow (45 km wide) extension of the continental shelf, with a shelf break at about 100 m. It has a complex hydrography: the waters of the bight are derived from the fast-flowing, southward-trending Agulhas Current, which is fed mostly by the tropical and subtropical surface waters of the South-West Indian Ocean subgyre, which are generally oligotrophic in nature, notably depleted in reduced nitrogen and phosphate except at river mouths and during periodic upwelling of deeper nutrient-rich water. Despite this, the bight is believed to be relatively productive, and it is suggested that efficient nutrient recycling by prokaryotes may sustain primary productivity efficiently, even in the absence of new nutrient inputs. Here we have measured bacterial numbers, biomass and heterotrophic productivity during summer and winter in conjunction with phytoplankton standing stock and factors that influence it. Bacterial distribution closely matched phytoplankton distribution in surface waters, and was highest close to the coast. Bacterial standing stocks were similar to those of oligotrophic systems elsewhere (0.5–5.0 × 10⁵ cells ml^–1; 1 × 10^–8 to 1.25 × 10^–7 g C ml^–1) and increased in association with the development of phytoplankton blooms offshore and with inputs of allochthonous material by rivers at the coast. Heterotrophic productivity in summer was lowest in the far south and north of the bight (0.5 × 10^–10 g C ml^–1 h^–1) but higher close to the shore, over shallow banks, and in association with increased phytoplankton abundance over the midshelf (1.0–3.5 × 10^–9 g C ml^–1 h^–1). There were marked seasonal differences with lower bacterial standing stocks (5 × 10⁴ to 2 × 10⁵ cells ml^–1; 4–5 × 10^–9 to 1–2 × 10^–8 g C ml^–1) and very low bacterial productivity (4 × 10^–11 to 1 × 10^–10 g C ml^–1 h^–1) in winter, probably resulting from lowered rates of primary productivity and dissolved organic matter release as well as reduced riverine allochthonous inputs during the winter drought.  相似文献   

广西隆安都结剖面下石炭统都安组上部鲕粒类型及其地质意义          下载免费PDF全文

巩恩普  杨臻元  黄文韬  关长庆  张永利  苗卓伟  王立芙  李骁  王俊杰 《古地理学报》2021,23(1)

鲕粒是一类特殊的沉积颗粒,为古气候和古海洋环境的重要指示器。为了深入认识此类特殊颗粒的成因机制、形成环境及地质意义,对广西隆安地区都结剖面下石炭统都安组上部含鲕粒地层开展了古生物学、沉积学和岩相学研究。研究区共识别出5种主要的鲕粒类型:放射状纹层鲕粒(O1)、规则同心放射状纹层鲕粒(O2)、不规则同心放射状纹层鲕粒(O3)、泥晶鲕粒(O4-A和O4-B)和复合鲕粒(O5)。各类鲕粒的显微组构和沉积环境指示其具有不同的形成过程,其中水动力条件影响和控制着鲕粒的发育和分布情况。研究区含鲕粒地层形成于维宪期末-谢尔普霍夫期,恰好对应早石炭世晚期冰川作用的开始。受冰川作用影响,全球海平面频繁波动,研究区地处低纬度地区并以浅滩和潮坪沉积环境为主,为鲕粒的形成提供了适宜的水体条件,即温暖、动荡的浅水环境。此外,含鲕粒岩层内广泛发育钙质微生物和微生物席,说明微生物活动在研究区较为常见,可能与鲕粒的形成过程具有一定的关联。  相似文献   

川西北下二叠统栖霞组微生物丘的发现及地质意义^*     

全子婷  谭秀成  张本健  唐浩  罗冰  杨迅  张亚  肖笛  汤艳玲 《古地理学报》2021,23(6):1110-1124

通过野外露头与钻井剖面的室内外分析,确认在四川盆地西北部(川西北)下二叠统栖霞组中发育有微生物丘,它们主要由凝块灰(云)岩、叠层灰(云)岩和微生物粘结颗粒灰(云)岩等组成。这些微生物碳酸盐岩发育较为典型的凝块、叠层、窗格、粘结等组构。微生物丘大小不一,实测高度一般为几十厘米至几米,宽度通常变化于几米至几十米之间,具有底平顶凸的典型丘形外貌,以发育向上变浅的沉积序列为特征,一般由丘基、丘核、丘盖3个微相组成,也可与颗粒滩共同构成微生物丘滩复合体。基于区域古地理背景和微生物丘特征的剖析,认为川西北地区栖霞期沉积环境总体受限,推测为半局限—局限台地环境,水深较浅,能量普遍不高;海平面频繁的相对升降变化和微生物丘的侧向迁移叠置,导致发育于缓坡背景下的碳酸盐岩台地极易受限,引起早期沉积物发生与丘滩发育密切相关的准同生期白云石化作用。因此,微生物丘滩复合体是栖霞组白云岩储集层发育的物质基础,台缘坡折带、台内缓坡折带和高地是栖霞组白云岩储集层发育的有利区带,这对寻找规模性层位不稳定的带状白云岩储集层具有重要的指导作用,并将大大拓展栖霞组白云岩储集层的勘探领域。  相似文献