对虾白斑综合症病毒极早期基因启动子筛选文库的构建     

林梵宇  徐丽美  杨丰 《台湾海峡》2010,29(2):184-188

对虾白斑综合症病毒（white spot syndrome virus,WSSV）是危害对虾养殖业的主要病原,而WSSV极早期基因对病毒功能基因的转录翻译和调控起着至关重要的作用.本研究将WSSV基因组以超声法随机断裂至400～900bp DNA片段,并插入启动子缺失且多克隆位点下游具有报告基因的载体来构建文库,转染昆虫细胞Hi5.倒置荧光显微镜观察发现部分转染后的细胞有增强型绿色荧光蛋白（EGFP）的表达,表明这部分细胞中的DNA片段可能具有极早期启动子活性.流式细胞仪分析显示,这部分荧光细胞占总细胞的比例为0.35%.由于转染效率为50%,可认为占比0.7%的WSSV基因组随机片段能在Hi5细胞中激发下游报告基因的转录翻译,可能具有极早期基因启动子活性.这对于进一步筛选和鉴定WSSV极早期基因及开展极早期启动子研究具有重要的意义.  相似文献   

调频加网中的随机问题   总被引：2，自引：0，他引：2  

苏永宪 《测绘学院学报》2001,18(3):226-228

调频加网是印刷中最新发展的一项极其重要的技术，它对于提高印刷品质量有着非常重要的作用。随机数产生的速度和质量决定着调频网点的质量，文中主要讨论了调频加网算法中随机数的产生以及随机数发生器的设计，重要讨论了乘同余随机数发生器。  相似文献   

Seismic vulnerability assessment of earthquake-prone mega-city Shillong,India using geophysical mapping and remote sensing     

Saurabh Baruah  Goutam Kashyap Boruah  Sangeeta Sharma  Washim Akram Hoque  Timangshu Chetia  Chandan Dey 《Georisk: Assessment and Management of Risk for Engineered Systems and Geohazards》2020,14(2):112-127

ABSTRACT

The concept of seismic vulnerability is a yard-stick of damage estimation from a probable earthquake considering physical cum social dimension and enables a basis for decision-makers to develop preparedness and mitigation strategies. We aim at vulnerability assessment of the typical urban system of capital city Shillong situated on hilly terrain. High-resolution satellite imagery of Shillong facilitates analysis of building footprints, communication network, and open ground. Different building typologies are identified taking into account the building’s structural configuration assessed through a rapid visual survey of more than 15% of total residential households. Slope map demarcates the landslide-prone area through discrete elevation modelling. A methodology incorporating several parameters e.g. building typology, slope angle, shear wave velocity characteristics, geomorphology, and the number of occupants in correlation with a physical measurement of vulnerability is presented and is applied to estimate the dimension of vulnerability. Additionally, MASW survey indicates lithology up to 30?m deep along with the existence of stiff soil and rocks at different depths whereas resonant frequency is identified to be in the range of 6–8?Hz through H/V ratio. Integrating all, it is observed that more than 60% of Shillong city falls under moderate to higher vulnerability and the rest is less vulnerable.  相似文献   

Acclimatization and screening of a chloramphenicol-degrading strain     

Xuanwen LU Xueqin TAO Zhi DANG 《中国地球化学学报》2006,25(B08):115-116

Chloramphenicol （CAP） is an antibiotic commonly used in aquaculture as a prophylactic or disinfectant to prevent diseases, or as a chemotherapeutic agent to control diseases. This antibiotic is either spread directly in the aquatic environment or administered through medicated feeds. It may be directly exposed to or enter the environment by leaching from uneaten feeds or from the aquatic animals＇ excrements. The antibiotic also directly enters the environment through pharmaceutical wastewater. Toxicity of CAP to human bone marrow has been linked to fatal aplastic anemia. Antimicrobial residues enter the environment, which may also establish a selective pressure in favour of antimicrobial-resistant bacteria. The residues of antibiotics may pose potential risk to public health or increase the occurrence of antibiotic-resistant bacteria in the aquatic environment. Due to these perceived risk, treatment of CAP in farm animals intended for human consumption has been restricted. However, residue of CAP in the environment is still a concern. There have been some reports on the residual effects of CAP on aquaculture and on microbial resistance to CAP in certain environments. The biotransformation of CAP in aquaculture pond sediments has been reported, which indicated that salinity is one of the many environmental factors that affect the degradation of CAP. However, acclimatization and screening of dominant bacteria for biodegrading CAP have been rarely studied. This paper studied the microbial degradation of CAP. In order to screen the dominant bacteria for degrading, the source of bacteria was sampled from the aquaculture pond sediments of Baiyun Borough, Guangzhou, China. 60 mg/L CAP was used as a sole carbon source to screen CAP-degrading bacteria and the dominant bacteria were acclimated after 2 months.  相似文献   

甘肃武山地区地下流体化学与构造应力关系初探     

王非 《内陆地震》1996,10(3):263-268

结合水氧、应力观测资料对甘肃武山地区地下流体化学特征（水化学、气体化学）与构造应力关系进行了定量计算并给出了二者之间的数学关系。  相似文献   

DRAINMOD-S模型参数的全局灵敏度分析   总被引：1，自引：0，他引：1       下载免费PDF全文

俞双恩  王宁  于智恒  王君 《水科学进展》2015,26(2):271-278

为有效进行DRAINMOD-S模型参数的优选,更好地理解参数变化对模拟结果的影响,开展了模型参数灵敏度分析。以南通市九龙垦区暗管排水脱盐试验为例,采用Morris全局定性分析方法检测了DRAINMOD-S模型模拟土壤剖面含盐量时侧向饱和导水率K_sat、水动力弥散系数D、地表最大蓄水深度S_m、相对不透水层深度I_m、排水系数D_r及地下水初始埋深W 6个参数的灵敏度。结果表明:K_sat对模拟结果影响最为显著,D、S_m和D_r次之,而W与I_m影响最小;各个参数间的非线性作用存在差异,以K_sat最为显著。为保证模型模拟质量,对敏感性参数应提高现场测试精度,在模型运行时,对灵敏度大的参数应进行重点调整,同时也不可忽视非线性作用较强的参数,从而有效地指导模型的参数率定,提高模型的适用性。  相似文献   

一株高效褐藻酸降解菌的筛选、鉴定及其发酵条件的优化   总被引：1，自引：0，他引：1  

侯士昌  温少红  唐志红  崔玉琳  秦松 《海洋科学》2014,38(7):20-26

以褐藻酸钠为唯一碳源,从腐烂海带中筛选得到褐藻酸钠降解能力较强的菌株H4,经生理生化和16S rDNA序列分析鉴定该菌株属于交替单胞菌属(Alteromonas)。对菌株H4的发酵条件优化研究表明,H4的较优培养基组成为(w/v):褐藻酸钠0.6%、酵母粉0.5%、蛋白胨0.25%、NaCl 3%;较优培养条件为:培养温度28℃,初始pH7.5,接种量1.5%(v/v),培养时间48 h。Fe3+对交替单胞菌H4的产酶有明显的促进作用,这在其他褐藻酸裂解酶生产菌株中未见报道,而Cu2+对褐藻酸裂解酶的抑制作用高达45.78%。在优化后的培养条件下,粗酶液酶活达到146.45 U/mL,较优化前提高了39.4%。  相似文献   

双排弹性桩隔振屏障对平面SH波的多重散射     

夏唐代  孙苗苗  华伟南 《世界地震工程》2011,27(1)

提出了一种新的求解任意排列、任意半径的弹性桩对平面SH波的多重散射的理论方法,以解决以往假设单重散射的计算方法中不考虑桩列作为整体屏障从而忽略桩间相互干涉关系的不足,并且可用于分析多排弹性桩对平面SH波的散射性状。在数值计算分析中讨论了散射重数,排间距,桩土剪切模量比,桩数等对双排弹性桩屏障隔离效果的影响,从而对实际工程中利用排桩进行振动污染的治理和屏障隔振设计提出了重要的理论依据。  相似文献   

Optimization of the OCO-2 Cloud Screening Algorithm and Evaluation against MODIS and TCCON Measurements over Land Surfaces in Europe and Japan     

Sijie CHEN  Shuaibo WANG  Lin SU  Changzhe DONG  Ju KE  Zhuofan ZHENG  Chonghui CHENG  Bowen TONG  Dong LIU 《大气科学进展》2020,37(4):387-398

A method to tighten the cloud screening thresholds based on local conditions is used to provide more stringent schemes for Orbiting Carbon Observatory-2(OCO-2)cloud screening algorithms.Cloud screening strategies are essential to remove scenes with significant cloud and/or aerosol contamination from OCO-2 observations,which helps to save on the data processing cost and ensure high quality retrievals of the column-averaged CO2 dry air mole fraction(XCO2).Based on the radiance measurements in the 0.76μm O2A band,1.61μm(weak),and 2.06μm(strong)CO2 bands,the current combination of the A-Band Preprocessor(ABP)algorithm and Iterative Maximum A Posteriori(IMAP)Differential Optical Absorption Spectroscopy(DOAS)Preprocessor(IDP)algorithm passes around 20%-25%of all soundings,which means that some contaminated scenes also pass the screening process.In this work,three independent pairs of threshold parameters used in the ABP and IDP algorithms are sufficiently tuned until the overall pass rate is close to the monthly clear-sky fraction from the MODIS cloud mask.The tightened thresholds are applied to observations over land surfaces in Europe and Japan in 2016.The results show improvement of agreement and positive predictive value compared to the collocated MODIS cloud mask,especially in summer and fall.In addition,analysis indicates that XCO2 retrievals with more stringent thresholds are in closer agreement with measurements from collocated Total Carbon Column Observing Network(TCCON)sites.  相似文献   

强电荷屏蔽下的电子俘获率     

罗志全  刘门全  林理彬  彭秋和 《天文学报》2005,46(3):253-257

分析了电荷屏蔽对超新星前身星环境下的电子俘获反应的影响，分析中强电荷屏蔽势能采用了最近人们利用线性响应理论给出的结果，对核素^56Co，^56Fe，^56Mn电子俘获率的分析计算表明，在低温高密情形下，电荷屏蔽对电子俘获反应的影响较先前的分析的影响程度略小，但电荷屏蔽对电子俘获反应的影响仍然显著。  相似文献