Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water     

Aridgides LJ  Doblin MA  Berke T  Dobbs FC  Matson DO  Drake LA 《Marine pollution bulletin》2004,48(11-12):1096-1101

There is enormous potential for global transfer of microorganisms, including pathogens, in ships' ballast water. We contend that a major advancement in the study of ballast-water microorganisms in particular, and of aquatic pathogens in general, will be expedited sample analysis, such as provided by the elegant technology of DNA microarrays. In order to use DNA microarrays, however, one must establish the appropriate conditions to bind target sequences in samples to multiple probes on the microarrays. We conducted proof-of-concept experiments to optimize simultaneous detection of multiple microorganisms using polymerase chain reaction (PCR) and Southern hybridization. We chose three target organisms, all potentially found in ballast water: a calicivirus, the bacterium Vibrio cholerae, and the photosynthetic protist Aureococcus anophagefferens. Here, we show simultaneous detection of multiple pathogens is possible, a result supporting the promising future use of microarrays for simultaneous detection of pathogens in ballast water.  相似文献   

Distribution of Bacteria Injected in Body of Giant Black Shrimp, Penaeus Monodon     

郭志勋  Karin van de Braak  Magriet Botterbloom 《中国海洋湖沼学报》2004,22(2)

1　INTRODUCTIONThepenaeidshrimpcultureindustriesintheworlddevelopedfromtheirexperimentalbeginningslessthanthirtyyearsagointoamajoraquaculturein dustrytodaythatprovideshundredsofthousandsofjobs,billionsofU .S.dollarsinrevenue,andaug mentstheglobalfoodsupplywithahighvaluecrop(LighterandRedmam ,1 998) .Upto 1 995,farmedshrimpatvolumeof71 2 0 0 0MT ,almostallconsistingofPenaeidspecies,accountedfor 2 7%ofworldshrimpproduction.Inthatyear,asoneofthemostimportantculturedshrimp,theproductionofP…  相似文献   

副溶血弧菌菌株1211U的活的非可培养状态   总被引：3，自引：2，他引：1  

姚斐  寇运同  陈刚  纪伟尚  徐怀恕 《海洋科学》2000,24(6):52-54

将副溶血弧菌（Ｖｉｂｒｉｏ ｐａｒａｈａｅｍｏｌｙｔｉｃｕｓ）菌株１２１１培养于低温贫养条件下,以平板菌落计数法（ＰＣ）和最大近似数法（ＭＰＮ）检测可培养细菌数,５０￣６０ｄ后表明可培养数下降为零。吖啶橙荧光显微镜直接计数法（ＡＯＤＣ）检测细菌总数,表明细菌总数始终变化不大,而活菌直接计数法（ＤＶＣ）检测到的活菌数保持在１０＾５个／ｍｌ。本实验证明了副溶血弧菌１２１１Ｕ株在一定的条件下或进入活的非  相似文献   

First isolation and characterization of a new species of Vibrio from diseased flounder (Paralichthys olivaceus Temminck et Schlegel)     

ZHANG Xiaojun  FANG Hai  CHEN Cuizhen  ZHAN Wenbin  JIN Xiaomin  WANG Xiuyun 《海洋学报(英文版)》2005,24(3):131-140

Appropriate pathogenic bacteria were isolated from diseased (or dead) flounder (Paralichthys olivaceus L) occurring in a mariculture farms of Qinhuangdao, which all diseased fishes expressed bacterial septicaemia. The phenotypic information of the 12 pure cultures was studied extensively, including morphological characteristics, colony characteristics in different media, physiological and biochemical characteristics, and the mole fraction G C ratio of the DNA for representative strain. The results show that the isolates belong to a new species of Vibrio, and are designated as Vibrio qinhuangdaora sp.nov, by the area from which the diseased flounders are collected (Qinhuangdao) based on its biological properties following Rules of International Code of Nomenclature of Bacteria. At the same time,studies on the serum homology ofisolates, and pathogenicity of isolates were conducted by experimental infection. The results show that all the isolates are of serologic similarity, and the isolates have strong pathogenicity to flounder. The studies can extensively reflect the main biological properties of this new species of Vibrio.  相似文献   

鳗弧菌对魁蚶血细胞形态及免疫功能的影响   总被引：3，自引：1，他引：2  

周丽青  杨爱国  王清印  吕振明  刘志鸿  田吉腾  吴彪 《海洋与湖沼》2014,45(3):536-541

本研究采用快速瑞氏-吉姆萨染色法观察鳗弧菌(Vibrio anguillarum)刺激后魁蚶(Scapharca broughtonii)几种血细胞的形态变化,研究不同种类血细胞的免疫功能。魁蚶血淋巴液中红细胞、白细胞和血栓细胞经免疫刺激后均发生形态改变,属于免疫防御的应激反应。经鳗弧菌免疫刺激后,红细胞无丝分裂现象增多,并参与病原菌的吞噬作用;白细胞变形运动、吞噬和融合现象普遍,细胞表面具有粘性,参与血栓的形成;血栓细胞主要表现出粘连和凝血的特性,偶有吞噬病原菌的现象;研究中检测了2种常规抗凝剂的抗凝效果,结果表明,2种抗凝剂分别改变了血栓细胞和白细胞的表面粘连特性,从而阻止它们参与凝血,因而都具有抗凝效果。本研究结果表明,魁蚶血细胞分工明确,能共同防御病原菌的入侵,细胞免疫在魁蚶血淋巴液抗菌免疫中起极其重要的作用,血细胞的形态变化和免疫功能分析可为后期研究魁蚶抗菌机制和筛选抗凝剂奠定基础。  相似文献   

海南地区溶藻弧菌遗传多样性的RAPD分析     

欧阳吉隆  周永灿  吴学贵  戴小连  王世锋  谢珍玉 《海洋科学》2011,35(7):30-36

以来源于泰国的菌株TG06003作对照,对采自海南地区的27株溶藻孤菌（Vibrio alginolyticus）环境菌株进行了随机扩增多态性DNA标记（RAPD）分析,其中6株为毒力菌株,另外22株为非毒力菌株。实验共得到70个多态性条带,多态性比率（PP13）为98．6％。基因多样度的算术平均数和加权平均数分别为0...  相似文献   

Influence of Dietary Probiotic Bacillus TC22 and Prebiotic Fructooligosaccharide on Growth,Immune Responses and Disease Resistance against Vibrio splendidus Infection in Sea Cucumber Apostichopus japonicus     

ZHAO Yancui  MAI Kangsen  XU Wei  ZHANG Wenbing  AI Qinghui  ZHANG Yanjiao  WANG Xiaojie  LIUFU Zhiguo 《青岛海洋大学学报(英文版)》2011,(3):293-300

  相似文献   

A VIBRIO ANGUILLARUM STRAIN ASSOCIATED WITH SKIN ULCER ON CULTURED FLOUNDER, PARALICHTHYS OLIVACEUS     

莫照兰  谭训刚  徐永立  张培军 《中国海洋湖沼学报》2001,(4)

ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｃｒｅａｓｉｎｇａｔｔｅｎｔｉｏｎｗａｓｆｏｃｕｓｅｄｏｎｔｈｅｎｅｅｄｔｏｓｔｕｄｙｔｈｅｂａｃｔｅｒｉｏｌｏｇｙｏｆｍａｒｉｎｅｆｉｓｈｉｎＣｈｉｎａ ,ｂｅｃａｕｓｅｏｆｂａｃｔｅｒｉａａｓｓｏｃｉａｔｉｏｎｗｉｔｈｈｉｇｈｍｏｒｔａｌｉｔｙｉｎｍａｒｉｎｅｆｉｓｈｃｕｌｔｕｒｅａｎｄｃｏｎｓｅｑｕｅｎｔｅｃｏｎｏｍｉｃｌｏｓｓｔｏｆｉｓｈｆａｒｍｓ (Ｄｅｎｇｅｔａｌ.,1 996;Ｌｉｎｅｔａｌ.,1 999) .Ｖｉｂｒｉｏｇｅｎｕｓｂａｃｔｅｒｉａｈａｖｅｅｍｅｒｇｅｄａｓｔ…  相似文献   

A VIBRIO ANGUILIARUM STRAIN ASSOCIATED WITH SKIN ULCER ON CULTURED FLOUNDER, PARALICHTHYS OLIVACEUS   总被引：2，自引：2，他引：0  

Mo Zhao-lan  Tan Xun-gang  Xu Yong-li  Zhang Pei-jun 《中国海洋湖沼学报》2001,19(4):319-326

The characteristics of a bacterium strain M3, isolated from cultured flounderParalichthys olivaceus with remarkable external sign of skin ulcer during an epizootic outbreak, indicated that the bacterium belonged to the speciesVibrio anguillarum. Challenge by I.M. (intramuscular injection), bath, and oral administration with M3 showed that it was highly pathogenic forParalichthys olivacues. The LD₅₀ dose was 5.144×10³ CFU/ per fish infection by I.M. injection. Recovered inoculated bacteria from the surviving fish revealed that the asymptomatic carriers could be a latent contagious source. Study of the effect of bacterial culture CFS (cell-free-supernatant) showed that the exotoxins produced by M3 play an important role in its pathogenicity for flounder. The resistance of M3 to 36 out of 41 antibiotics indicated that the bacterial disease outbreak was mainly attributable to the frequent and excessive use of antimicrobial agents; and that vaccination would be an effective precaution against bacterial disease. Contribution No. 4107 from Institute of Oceanology, Chinese Academy of Science. Contribution No. 270 from Experimental marine Biology Laboratory, CAS. Funded by projects under the Major State Basic Research Development Program, Grant G1999012003.  相似文献   

Interaction of Haematopoietic Tissue Cultures of the Dublin Bay Prawn,Nephrops Norvegicus(L.),with the Causal Agent of Luminous Vibriosis Vibrio Harveyi     

Mulford A.L  ZHANG X.H  XU H.S  Austin B 《中国海洋大学学报(英文版)》2002,1(1)

Vibrio harveyi cells (dose = ＞ 103 cells mL 1) and extracellular products (ECP; ＞25μg m L-1 of total protein concentration) destroyed haematopoietic cultures of Nephrops norvegicus within 24 h of exposure. Cytopathic effects (CPE)started after 4 h of exposure to the bacterial cells, with some granularity in the cytoplasm, mostly in cells in the outer periphery of the explant growth. At the end of the infection, a considerable number of nuclei remained attached to the substrate,apparently unaffected. Following exposure to ECP, initial deterioration was observed at 2 h with the presence of granularity in the cytoplasm of＜ 20% cells, and few cells displayed small vacuoles around the nuclei. Parallel results were obtained using whole animal experiments, with V. harveyi cells being lethal to nephrops within 24 h.  相似文献