Development of EST-SSR primers and their practicability test for Laminaria     

WANG Guoliang  TAN Xiaolong  SHEN Junling  LI Jian  ZHANG Lei  SUN Jianwei  WANG Bin  WENG Manli  LIU Tao 《海洋学报(英文版)》2011,30(3):112-117

Laminaria (Saccharina) is one of the economic important seaweeds.Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software,two hundred and fifty-four SSRs in 206 ESTs were found.From the 254 SSRs,sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most (more than 80%) of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines,which were widely used in breeding and economic cultivation in China,was performed based on the obtained SSR data.  相似文献   

Development of EST-SSR primers and their practicability test for Larninaria     

WANGGuoliang  TANXiaolong  SHENJunling  LIJian  ZHANGLei  SUNJianwei  WANGBin  WENGManli  LIUTao 《海洋学报(英文版)》2011,30(3)

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黄口荔枝螺转录组数据的微卫星标记开发与分析     

李威  赵姗  焦海峰  林志华  包永波 《海洋科学》2015,39(11):61-67

以黄口荔枝螺转录组测序所得到的拼接序列为基础,利用MISA软件进行微卫星分析,利用Primer5.0设计引物160对。结果显示,83对引物能够成功扩增,引物在黄口荔枝螺渔山列岛群体多态性检测中发现,37个SSR位点能够表现出多态性,一共获得了129个等位基因,各位点等位基因数为2~5个,平均每个位点等位基因数为3.49个,观测杂合度H_o,期望杂合度H_e,多态信息含量PIC范围分别介于0.000~0.800、0.146~0.645、0.139~0.573之间,有9个表现为高度多态,20个表现为中度多态,8个表现为低度多态。实验结果进行Hardy-Weinberg平衡检测后,利用Bonferroni correction法进行校正,有29个位点显著偏离,结果表明渔山列岛黄口荔枝螺群体总体遗传多样性水平较低。  相似文献   

虾夷扇贝选育群体与野生群体基因组DNA甲基化的MSAP分析   总被引：1，自引：0，他引：1  

吴彪  杨爱国  董迎辉  郑利兵  刘志鸿  周丽青  孙秀俊  郑言鑫 《海洋与湖沼》2015,46(4):976-982

基因组DNA甲基化作为表观遗传学研究的重要手段之一,在水产科学领域得到越来越广泛的应用。为从表观遗传学角度探讨虾夷扇贝选育群体-"玉贝"和普通虾夷扇贝群体的DNA甲基化差异,本文运用甲基化敏感扩增多态性(Methylation-sensitive amplification polymorphism,MSAP)技术分析了两群体的DNA序列中CCGG位点的甲基化情况。结果表明,筛选得到11对引物组合能够在两个群体中得到稳定扩增、清晰且多态性丰富的片段;11对引物在"玉贝"和对照群体中分别共检测到1432和1442个位点,其中发生甲基化的位点分别为306和341个,总甲基化率分别为21.5%和23.65%,"玉贝"总甲基化率下降2.15%;全甲基化位点分别为152和236个,全甲基化率为10.68%和16.37%,"玉贝"全甲基化率下降5.69%;半甲基化位点分别为154和105个,半甲基化率为10.82%和7.28%,"玉贝"半甲基化率上升3.54%;"玉贝"群体的甲基化多态性条带所占比例高于对照群体,分别为31%和29.167%,尤其是B类型条带链,"玉贝"群体明显高于对照群体。这说明,通过对虾夷扇贝生长和耐热性状的累代选育导致了DNA甲基化水平和模式的改变,本研究为进行虾夷扇贝抗逆基因的筛查提供了参考数据,丰富了表观遗传学在扇贝中的研究资料。  相似文献   

Microsatellite DNA polymorphisms and the relation with body weight in sea cucumber <Emphasis Type="Italic">Apostichopus japonicus</Emphasis>     

王秀利  单雪  仇雪梅  孟祥盈  常亚青 《中国海洋湖沼学报》2009,27(2):331-336

The relationship between microsatellite polymorphism and body weight of captive bred Chinese sea cucumber Apostichopus japonicus was investigated in two local populations in Dalian. Among ten loci discovered, nine show changes except for AJ07 loci. Seven loci were found highly polymorphic in both populations. For each locus in two populations, the average number of alleles is 6.428 6 and 6.285 7, the average observed heterozygosity at 0.225 7 and 0.245 9, the expected heterozygosity at 0.776 8 and 0.748 8, the polymorphism information content (PIC) at 0.709 2 and 0.674 6, respectively. Further analysis show significant correlation between A. japonicus body weight and occurrence markers AJ02 and AJ04. The findings of the relation may be helpful for molecular breeding, as well as the marker-assisted selection of sea cucumbers. Supported by the National High Technology Research and Development Program of China (863 Program, No. 2006AA10A411), the Natural Science Foundation of Liaoning Province (No.20072139), the Grant of Dalian Fisheries University, the Key Laboratory Foundation of the Educational Department of Liaoning Province (No.2009S024)  相似文献   

Isoenzymes and soluble protein in Arthrospirafrom alkaline lakes in Erdos Plateau, China and in exotic species   总被引：1，自引：0，他引：1  

杨茜栗淑媛  扈瑞平刘燕 乔辰 《中国海洋湖沼学报》2006,24(2):134-141

1 INTRODUCTION Since isoenzyme was first named by Markert and M?ller in 1957 (Hu and Wan, 1985), the separa- tion of isoenzyme has been rapidly developed. Isoenzymes is an important tool for studying life in molecule level. Isoenzymes are transcribed by g…  相似文献   

面向对象技术在基于模板的冷冲模CAD系统中的应用     

王军  肖冬荣  水泱 《南京气象学院学报》2003,26(5):702-706

面向对象技术是当今的主流软件开发方法，它具有封装性、继承性和多态性。讨论了面向对象技术的封装性和多态性在基于模板的冷冲模CAD系统中的应用，分析它们在提高系统可扩充性、降低系统复杂度方面的作用，并举例分别说明。  相似文献   

Molar refraction of quartz family minerals     

S. K. Nataraju  D. Krishnamurti 《Journal of Earth System Science》1984,93(3):309-317

Different polymorphs of silica and the opals exhibit significant variations in densities. The paper discusses and correlates the variations in the molar refraction with density. It emerges that in different polymorphs of silica, the increase in density involves a corresponding increase in the characteristic dispersion frequencies leading to a decrease in the molar refraction. The variation of the molar refraction with density can be represented in terms of an elementary formula. The variation of the molar refraction of opals with the water content in them is also accounted for.  相似文献   

蟹类线粒体DNA的研究与应用   总被引：7，自引：1，他引：7  

徐敬明 《中国海洋大学学报(自然科学版)》2006,36(6):879-884

线粒体DNA作为理想的分子遗传标记已被广泛用于蟹类种群遗传学和进化遗传学的研究,并取得了许多有意义的结果。本文阐述了蟹类线粒体DNA分子生物学的研究进展,重点介绍蟹类线粒体DNA序列的研究概况及其多态性在蟹类系统学、种群识别、起源和进化、地理分化等研究中的应用情况。  相似文献   

Genetic studies of redfish (Sebastes spp.) from Icelandic and Greenland waters     

Nedreaas  Kjell; Johansen  Torild; Naevdal  Gunnar 《ICES Journal of Marine Science》1994,51(4):461-467

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